Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,9100GC23.8% 448.9 / 35.2 255A664A (GCG→GCCrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base G (130/46);  new base C (52/3);  total (193/62)
Fisher's exact test for biased strand distribution p-value = 5.70e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

CAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCC  >  NC_000913/1708761‑1709059
                                                                                                                                                      |                                                                                                                                                     
caggcagagccGCGCAACACCGCCGTCGAAGCAGCTATCGCCCGCGCCAAAGCTCGCAAGCTGGAACAGCAACAGGATACTGCCGACCCAGAACAACAGGGCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAgcgc                                                                                                                                                      <  7:922444/142‑1 (MQ=11)
       aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTAGATCCGCGCAAAGCAGCACGCGAGCCCGGGCTTGCGCGAGCCACTGCGCGCcaagg                                                                                                                                               >  2:505396/1‑149 (MQ=11)
         ccGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTGGATCCGCGCAAAGCAGCAGTCGAAGCGGCTATTGCCCGCGCACAAGCGCGCAAACggg                                                                                                                                             >  4:1556060/1‑151 (MQ=14)
           gcgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGTGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCACGAGCTAATGCAAGAAAACGGGa                                                                                                                                            >  5:1339335/1‑150 (MQ=14)
           cccccacgccgccgccgccGCCCCTATCCCCCGCGCCAAAGCCCGCAATCTGGAACAGCAACAGGCTAATGCCGACCCAGACCTCCCGGTCGATCCGCGCAAAGCGGCCGGCGAAGCCGCTATCGCCCGAGCCAAAGCGCGCAAACGGGaa                                                                                                                                           <  1:1461744/144‑1 (MQ=2)
              cAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGACGCGGCTATTGCCCGCGCGAAAGCCCCAAAACgggccc                                                                                                                                          >  2:741141/1‑146 (MQ=14)
                 aGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTTGATCCGCGCAAAGCGCGCTGCGAAGCGCCTAATGCACGAGGCAACGCACCCAACCTGCAACCGCa                                                                                                                                      >  1:660429/1‑150 (MQ=11)
                    ccccgTCGCAGCCCCTATTGCCCGTGCCAAAGCTCTCAATCTGGAACAGCAACAGGCTAATGCCGATCCATAACATCAGGTCGATCCGCGCAAAGGCGCCGTCGAAGACGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCg                                                                                                                                  <  2:1834279/149‑1 (MQ=14)
                       cgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCAGCGCAAAGCGGCAGTAGCAGCCGCTATTGCCCGCGCCAAAGCCCGAAAACGCGAACAGCACCAGGCt                                                                                                                               >  5:826369/1‑151 (MQ=14)
                        gTCGAAGCCGCTATTGCCCGTTCCAAAGCCCGCAAGCTGGAACAGCAACAGTCTAATGCCGATCCAGAACAACAGGGCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTa                                                                                                                              <  1:920306/151‑1 (MQ=14)
                        gTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCAGCCGTAGCCGCGGCTATTGCCCGAGACCACTAGCGCGACCGGGAGCCGAAACCGGcgc                                                                                                                              >  4:480149/1‑149 (MQ=11)
                         tagAAGCCGCTAGTGCACGTGCCAAAGCTCGCAAGCGGGAACAGCAACAGCCTAATGCCGACCCAGAACCAGATGGCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTa                                                                                                                              <  3:90504/148‑1 (MQ=14)
                            aaGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTGGACGCGGCTATTGCCCGCGCGCACGCGCGCAAAGCGGCACAGCAGCAGGGTAAtcc                                                                                                                          >  7:176411/1‑149 (MQ=14)
                               ccGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGACGATCCTCGCACAGCCGCCGTCGCAGCCGCTATTGCCCGAGCCAAAGAGCGCAAACGGGCTCAGCAACCGGc                                                                                                                                >  7:1523917/1‑142 (MQ=17)
                                cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCCGCCGTCGACGACGCGATTGCCCGCGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCgg                                                                                                                        >  3:690421/1‑149 (MQ=14)
                                 gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCACGAGCCAAAGCGCGCAAGCGGGAACAGCAACCgggggctggggcgc                                                                                                                     >  3:1303139/1‑139 (MQ=14)
                                 gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGCGCAAACGCCAAAAAACCCGACCAGCAAAACGCAAATGCGGAGc                                                                                                                     >  8:1278407/1‑151 (MQ=14)
                                   tATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTCGCAGCCGCTATTGCCCGCGCGAAAGCCAGCAAACGGGCACAGCAGCCGGATATAGAGGAGcc                                                                                                                    >  6:812723/1‑150 (MQ=14)
                                    aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAGTGAGGCGCCAGCACAGCAGGGCGCTCCGCGCAAAGCAGCCGTCGAGGCGGCTAGTGCCCGAGACAAAGCGCGCACGCGGGACCCGCACCCGGCtcgggcggcggc                                                                                                                    >  3:1688255/1‑138 (MQ=9)
                                       gcccccgccaccgCACCCCAGCTGGAACAGCAACAGGCTAATGCCGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaag                                                                                                               <  3:87386/139‑1 (MQ=14)
                                        cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCGGCTATTGCCCGCGCGCAAGCCAGCAAAGCAGATCAGCAGCCGGATAATGAggtaaaaga                                                                                                                 >  4:1490921/1‑141 (MQ=14)
                                        cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGCGCCAAAGCCAGCAAACGCGAACAGCAAAAGGATAAtcacgaaccaca                                                                                                                 >  6:825885/1‑137 (MQ=14)
                                            tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGCCCGCGGAAGAGCACCGGTCTCCGCTCCACGCAGCCCTCGATGGCGCTATTGCCCGCGCCAGCGGGCGCAAACGGGAACCGCCCCCGGGTAcggcgggggcggctgcggcg                                                                                                          >  5:735790/1‑137 (MQ=2)
                                                    cACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCGGCTATTGCCCGCGACACAGCACGCAAGAGGAAACAGAAACAGGGTAAAGATGTGAAAGAAGACCAGGTTGATcc                                                                                                  >  2:556669/1‑151 (MQ=2)
                                                     aCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGCGCTAAAGCCAACAAACCCGACCAGCAGCAGGATAAAGAGGAGCAAGAAGTACAGGTAGATCcg                                                                                                 >  4:447891/1‑151 (MQ=11)
                                                       gCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGAGCCCACGCGCGCAAACGGGAACAGCACCAGGCTAATGCGGAGCCAGAACAACAGGGTGATCcgcc                                                                                               >  3:359658/1‑150 (MQ=14)
                                                         aaGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGACGCCGCTATTGCCCGCGCGCAAGCGCGCAAACGCGCACAGCAGCAGGCTAATGCGGAGCCAGAAGAGCAGGtagctaagcga                                                                                              >  2:275910/1‑140 (MQ=14)
                                                         aaGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTCGACGCCGCTATTGCCCGAGCGAAAGCCCGCAAACGGGACCAGCACCCGGCTAAAGCCGAGCCAGAACACCACGTTGCTCCgcgc                                                                                              >  1:551489/1‑150 (MQ=14)
                                                         aaGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCAAAAGCCCAAAAACGCGAACAGCAACAGGATAAAGCGGAACCACAACAACATGTTGATCCgcgc                                                                                              >  6:484476/1‑150 (MQ=14)
                                                             tGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGCGAACAGCACCAGGCTCTTCCGGAGCCAGAAGAACAGGTTGCTCCCCGCACAGc                                                                                         >  1:1001528/1‑151 (MQ=14)
                                                             tGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAACGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGACCAGGTGGATCAGCGCAAAGc                                                                                         >  5:468971/1‑151 (MQ=14)
                                                             tGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTCGACGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGACCAGGTTCCTCCGCGCACAGc                                                                                         >  1:113990/1‑151 (MQ=14)
                                                              ggAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGGTCCGCGCAAAGCCGCAGGCCAAGCCGCTATTGCCCGAGCCAAATCCCGCAATCTGGAACAGAAACAGGCTAATGCGGGGGCAGAAGCGCAGGTGGGTCCgggccccggg                                                                                        >  1:1273604/1‑145 (MQ=11)
                                                                 aCAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGcc                                                                                     >  1:614944/1‑151 (MQ=255)
                                                                 aCAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCAAAAGCCCGCAAACGGGCACAGCAACAGGCTAATGCGGAGCCAGAAGACCAGGTCGCACCGCGCACAGCGGcc                                                                                     >  3:1177598/1‑151 (MQ=14)
                                                                 aCAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGCGCTAAAGCCAGAAAACCCGAACAGCAACAGGATAAAGACGAGCAAGAAAAACAGGTAGATAAACCAAAAGCTGcc                                                                                     >  2:498165/1‑151 (MQ=255)
                                                                      aaCAGGCTAATACCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGCAGCCGCTATTGCCCGAGCAAAGGCCAGCAACCCGGACCAGCACCAGGATAAAGAGGAGCAAGAAGAGGAGGTGCCACCGCGCAAAGCGGCCgatgc                                                                                >  2:1704507/1‑147 (MQ=2)
                                                                       aCAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCACCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGac                                                                               >  7:167649/1‑150 (MQ=255)
                                                                        cAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCAAAAGCCCGAAAACGCGAACAGCAGCAGGCTAATGCGGAGGCATAAGAACAGGTAGATCCGCGCAAAGCTGAGGTCGa                                                                                >  2:214667/1‑149 (MQ=14)
                                                                               aTGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTTGCAGCGGCTAt                                                                       >  4:207429/1‑151 (MQ=255)
                                                                               aTGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACGGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGATGATCCGCGCAAAGCTGCCGTAGACGCGGCTAt                                                                       >  6:271203/1‑151 (MQ=14)
                                                                                tGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGAACCGAGCAAAGCTGCCGTAGCAGCGGCTAtt                                                                      >  2:1297866/1‑151 (MQ=17)
                                                                                   cGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACACGTTGATCCGCGCAAAGCTGCAGTGGAAGCGGCTATTGcc                                                                   >  4:427735/1‑150 (MQ=255)
                                                                                    gAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAc                                                                  >  5:1786790/1‑151 (MQ=255)
                                                                                     aGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGTGCCAGAAGAACAGGGTGATCCGCGCAAAGCTGCAGTGGACGCGGCTATTGCAcg                                                                 >  5:573482/1‑151 (MQ=17)
                                                                                           cccaccaccgcgctCCGCGCAACCCCGCCGTCGAAGCCGCTATCGCCCGCGCCAAAGCGCGCAACCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCaa                                                           <  7:514661/138‑1 (MQ=17)
                                                                                            aaaccaGGTCTCTGCGCTCAAAGCCGGCGACGACGCGGCTATTGCCATATCCAAAGCCCGCAATCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCaaa                                                          <  7:253505/147‑1 (MQ=18)
                                                                                              aacccgaccaTCAGCGCACAGCCGCCGTCGCACCCGCGATCGACCGAGCCAAAGCGCGCAACCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGc                                                        <  3:1316707/142‑1 (MQ=21)
                                                                                              aacaGGTGGATCCTAGCAAAGCCGCCGTAGAAGCCGCTATTGCGAGTGTCTAAGGTTGTAATCTGGAACAGCAACAGGCTAATGCGGATCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGc                                                        <  6:993391/151‑1 (MQ=11)
                                                                                              aacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAACCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGCTCGGCGCAAAGCTGCAGTATAAGAGTCTATTGCACGCGCCaa                                                           >  3:1537326/1‑148 (MQ=17)
                                                                                              aacaGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCAAAAGCCCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAATCACATGATGATCCGCGCAAAGCTGCCGTAGCCGCGTATATTGCACGCGCGAAGGc                                                        >  8:79920/1‑151 (MQ=14)
                                                                                                 aGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTGGCCGCGGCTAATGCCCGCGCCAACGCaa                                                      >  2:699819/1‑149 (MQ=14)
                                                                                                  ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCg                                                                                                                         >  7:398666/1‑82 (MQ=37)
                                                                                                      gATCCGCGCCAACCCGCCGTGGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAATCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAAGAGGTGGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGc                                                <  5:856729/151‑1 (MQ=255)
                                                                                                       ccccccccAACGCCGCCGTCGAAGCCTTTATTTAACGTTCCACACGTCGAAATCGTGAACACCAACCTGCTACTGTGTATCCATAATATCAGCTCTATCGTTGTAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCt                                               <  8:861725/144‑1 (MQ=255)
                                                                                                       aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCt                                               >  3:235394/1‑151 (MQ=255)
                                                                                                          cgcgCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTggc                                            >  8:476459/1‑150 (MQ=255)
                                                                                                           gcgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCCACGCACGCAACCAGGac                                           >  7:773381/1‑150 (MQ=255)
                                                                                                            cgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAACGCGCGCAACCGGGAACAGCAACCGGCTAATGCGGAGCCAGCAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCACGCGCCAACGCCCGCaaccgcgacc                                          >  2:1114051/1‑143 (MQ=17)
                                                                                                             gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAAc                                          >  3:781781/1‑150 (MQ=255)
                                                                                                             gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGAAGCGGCTATTGCACGCGCGAAAGCaacaaaacacgacc                                          >  4:1144110/1‑137 (MQ=17)
                                                                                                              cAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGACGCGGCTATTGCACGCGCCAAAGCACGCAAGCGCGAAc                                          >  2:1779058/1‑149 (MQ=255)
                                                                                                               aaaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGc                                       >  6:1030887/1‑151 (MQ=255)
                                                                                                               aaaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGAAAGCTCGAACacc                                       >  8:1175881/1‑149 (MQ=255)
                                                                                                               aaaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCACGCGCGAACGCACGCAAGCTCGAACAGc                                       >  1:249784/1‑151 (MQ=255)
                                                                                                                aaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGACCAGc                                       >  5:1016415/1‑150 (MQ=255)
                                                                                                                 aGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGACCAGCa                                      >  3:929688/1‑150 (MQ=255)
                                                                                                                  acctgccgTGGAAGCAGCTATCGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATg                                                                                                                           <  7:675994/61‑1 (MQ=255)
                                                                                                                   gcccgcgTCGCAGCCGCTATTGCCCGAGCCAAAGCTCTCAAACTGTAACAGCAACCGGCTAATGCTGAGCCAGAAGAACAGGTTTATCCGCGCAAACCTGCCTTCGAAGCTGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAAc                                    <  6:1306850/146‑1 (MQ=14)
                                                                                                                     cgccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCACCGCACGCAAGCTGGGCCAGGAAccg                                  >  7:1495924/1‑149 (MQ=17)
                                                                                                                     cgccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCacc                                    >  5:1061152/1‑147 (MQ=255)
                                                                                                                     ccccctCGAAGCCGCTATTGCCCGAGCCAAAGCTCTCAAACGGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAg                                  <  5:1494922/146‑1 (MQ=17)
                                                                                                                       ccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCCCGCGCCAAAGCCCGCAAGCTGGAACAGCAACAGGc                                >  6:620719/1‑151 (MQ=255)
                                                                                                                         ggcGACGCCCCTATTGCCCGAGCCAAAGCGCGCAAACTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGACGATCCGCGCAAAGCTGCCGGCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTa                              <  7:1234208/149‑1 (MQ=14)
                                                                                                                            gAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATg                           >  5:597489/1‑151 (MQ=255)
                                                                                                                                ccGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCACGCGCGAACGCACGCAACCTGGAACAGCAACAGGCTCATGAGGt                       >  5:281767/1‑151 (MQ=25)
                                                                                                                                ccGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAg                                                                              <  7:443547/96‑1 (MQ=255)
                                                                                                                                ccGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCggg                       >  2:1521862/1‑150 (MQ=255)
                                                                                                                                ccGCTATTGCCCGAACCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCGAACGCCCGCAAGCTGGAACAGCACCAGGCTCATGCGGt                       >  3:1124578/1‑151 (MQ=25)
                                                                                                                                 cGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGCCCAGCACCAGGCTAATGCGGtc                      >  8:1112621/1‑150 (MQ=255)
                                                                                                                                  gCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTGGACGCGGCTATTCCACGCGCAAAAGCCCGCAAGCGGGACCAGCAACAGGCTAATCCggacc                     >  8:662869/1‑148 (MQ=21)
                                                                                                                                   cTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAACGCACGCAAGCAGGAACAGCAACAGGCTAATGCGGTAcc                    >  8:1540303/1‑151 (MQ=255)
                                                                                                                                    caTTGCCCGAGCCAAAGTGCGCTAACGGAAACACCTACATACTAATGAGGCTCCTGAACCCGACGTTGAGCCGGGCCATCATGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCa                   <  1:736064/150‑1 (MQ=9)
                                                                                                                                     attgccctttacAAACCACGCAAACGCGAACAGCAACCTGCTAATGCCGAGCCATAACAACAGCGTGATCCGCTCACAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCag                  <  2:343895/140‑1 (MQ=17)
                                                                                                                                      ttGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCCACAGGCTAATGCGGTccc                    >  6:1664181/1‑148 (MQ=255)
                                                                                                                                      ttGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCACGCGCCAAGGCCCGAAAGGAGGCACAGCAACAGGATAATGCCGtcgacg                  >  4:985122/1‑145 (MQ=21)
                                                                                                                                       tGCCCGTGCCAAAGCGCGCAATCTGGAACAGCAACCGGCTAATGCGGATCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGc                                                        <  6:1454519/111‑1 (MQ=255)
                                                                                                                                       tGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCAAGCAAGCTGGACCAGCAACAGGCTAATGCGGGACCaga                 >  7:649216/1‑150 (MQ=255)
                                                                                                                                         cccGAGCCAAAGCGCGCAATCTTGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCTTCCGTCGAAGCGTCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaaga              <  6:729296/151‑1 (MQ=14)
                                                                                                                                          ccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGTACCagaaga              >  3:544138/1‑150 (MQ=255)
                                                                                                                                           cGAGCCAAAGCTCGCAAACGGTAACAGCAACCGGCTAATGCGGAGCCAGAAGATCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAAc            <  3:725266/151‑1 (MQ=255)
                                                                                                                                           cGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGATGCAGTGGAAGCGGCTATTGCACGCGCAAACGCACGCAAGCTGGAACAGCATCAGGCTAATCCGGTACAagaagacc            >  7:1051770/1‑149 (MQ=25)
                                                                                                                                            gAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGATAATGCGGTACCAGAAGAAc            >  8:306751/1‑150 (MQ=255)
                                                                                                                                             aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTGGCAGCGGCTATTGCACGCGCCAAGGCCCGCAAGCGGGCCCAGCAGCAGGCTGTTGCGGTGGCagaagtcg            >  8:139108/1‑146 (MQ=18)
                                                                                                                                              gCCAACGCTTGCAATCGGCTACCGTAATCGGCTAATGCGGATCCAGAAGAACAGTTTTATCCGCGCAAAGCTGACGTCGAAGCTTATATTTCACGGGCCAAAGCACGCAAGCTTGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAgg         <  6:429263/151‑1 (MQ=14)
                                                                                                                                                 aaaGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAACGCACGCAAGCTGGAACAGCAACAGCCTAATGCGGTACCAGAAGAACAGGttc      >  1:1826645/1‑150 (MQ=255)
                                                                                                                                                 aaaGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAAAAGGCTAATGCGGTTCCAGAAGATCAGGTTg      >  2:78551/1‑151 (MQ=255)
                                                                                                                                                  aaGCTCGCAAACGTGAACAGCAACAGGCTAATGCCGAGACAGAAGAACAGGTTTATCCTACAATAGCTTCCTTCGATCTGGCTATTGCACGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGa     <  6:692304/151‑1 (MQ=17)
                                                                                                                                                   aGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTCCCAGAAGAACAGGTTGAt    >  3:63519/1‑151 (MQ=255)
                                                                                                                                                     cgcgcAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAACCTGGAACAGCAACAGGCTAATGCCGTACCAGAAGAACAGGTTGATcc  >  4:693465/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCC  >  NC_000913/1708761‑1709059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: