Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1450TC23.3% 386.5 / 129.2 245intergenic (‑10/+53)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (55/106);  new base C (11/38);  total (98/147)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-02

Only 100 of 271 total aligned reads displayed.

AGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTT  >  NC_000913/2817997‑2818291
                                                                                                                                                     |                                                                                                                                                      
aGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAat                                                                                                                                                        <  1:1172695/149‑1 (MQ=40)
 gTGCCTGCTGTTAATTCCTCTACCTCTTCCATCCCCTCATTGCTGCATATGACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAactta                                                                                                                                                     <  3:1058796/151‑1 (MQ=11)
  tGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatttat                                                                                                                                                    >  1:950746/1‑151 (MQ=255)
     cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTTCTGAAGATTTCGGAGAATATCGTGCATACGGCAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAtttt                                                                                                                                                 >  6:1746649/1‑151 (MQ=255)
       cctttttaTTCCTCTAGATCTTCGATACGTTCATTCCTGAAGATTTCGTAATATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTAc                                                                                                                                               <  4:1476205/144‑1 (MQ=14)
        gggTTAATTCCGCTATCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTAct                                                                                                                                              <  4:1153199/150‑1 (MQ=255)
        cGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCACCTTAGCTACAGAAGAAGAGTG‑AAATGGATTTTAct                                                                                                                                              >  7:1534632/1‑151 (MQ=14)
        cGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGCTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTAct                                                                                                                                              <  7:573844/151‑1 (MQ=9)
        agtttAATTCCCCGACATATTGTATACGTTAATTTCTGAATATTTAGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACttactttact                                                                                                                                              <  7:1147834/148‑1 (MQ=11)
          gTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgc                                                                                                                                            <  7:1353908/151‑1 (MQ=255)
             aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGTATGCATCGGG‑ATATTTATTTTActtctg                                                                                                                                          >  5:330195/1‑150 (MQ=255)
             aaTACCGCTATCTCTTCGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa                                                                                                                                         <  8:549225/151‑1 (MQ=9)
              gtgcgcttccgagctCGATACCTTCATTGCTGAAGCTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa                                                                                                                                         <  3:329738/136‑1 (MQ=11)
              aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑t                                                                                                                                      <  7:463090/151‑1 (MQ=255)
              aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAACTACGCAAGAGCGCTTCTTAGCCGAGTAGTCTCTCCAGATGAGCTACGTATGCCTCGGG‑ATATTTAATTAACTGCTGa‑‑t                                                                                                                                      >  5:1780364/1‑151 (MQ=14)
              aTTCCGCGACCTCCTCCAACCCTTCCTAGCTGAAGATCTCGGAGAATATTTTTCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑t                                                                                                                                      <  5:1269222/151‑1 (MQ=14)
                tcctggagctctTGCATCCCGTCATTCCGGAATATTTCGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑tt                                                                                                                                     <  3:1294621/142‑2 (MQ=1)
                tcctctagatctTCGTAACCTTCCTTGCTGAAGATTTCGGAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑tt                                                                                                                                     <  8:1214664/141‑2 (MQ=11)
                tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGa‑‑ta‑‑t                                                                                                                                  >  5:1490535/1‑151 (MQ=255)
                tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGa‑‑ta‑‑t                                                                                                                                  >  1:1211741/1‑151 (MQ=255)
                 ccGCTACCTCTTCGATACCTTCATTGCTGACGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑tt                                                                                                                                 <  1:424628/151‑1 (MQ=255)
                 ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑tt                                                                                                                                 <  3:565444/151‑1 (MQ=255)
                 ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑tt                                                                                                                                 <  4:415629/151‑1 (MQ=255)
                 actcTACCTCTTGTTTACGTTCATTTATGAAGATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tt‑‑tt                                                                                                                                 <  2:66908/148‑1 (MQ=11)
                   gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGa                                                                                                                               <  2:1240503/151‑1 (MQ=255)
                    gttcatcttgcttACCCTCATTGATGAAGATATCGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tt‑‑ttt                                                                                                                                <  1:1688806/137‑2 (MQ=11)
                    cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAGTTTACTGCAGA‑‑TA‑‑TTTAt                                                                                                                              >  2:1551636/1‑151 (MQ=255)
                    cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGAATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAATTTACTGCTGa‑‑ta‑‑t                                                                                                                                  >  8:1537872/1‑147 (MQ=255)
                      atctctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActagatcgtaagag                                                                                                                                  >  2:1431744/3‑137 (MQ=11)
                      aCCTCTTCGATCCCTTCATTGCTGAAGAGATAGTAGAATATGGTGCTTCCGGCAGGATTCGAACCTCCGACCGATCGGTTCGTAGCGGAGTACTCTATCCAGCTGAGCTACGGATTGATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGAt                                                                                                                              <  3:931744/149‑1 (MQ=14)
                      aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATAc                                                                                                                            >  7:298244/1‑151 (MQ=255)
                      aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACATATGAAGAGCG‑AAATGTATTTAAATACTGa‑‑taatcaaac                                                                                                                              >  8:1279070/1‑144 (MQ=11)
                       catctTCCTTACCTTCCTTTCTGAATATTTAGTATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tt‑‑ttttgata                                                                                                                           <  6:120768/149‑7 (MQ=11)
                       catattgtaTACGTTAATTTATGAATATTTAGTAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tt‑‑ttttgat                                                                                                                            <  6:314768/142‑6 (MQ=11)
                        ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑TA‑‑TTTATATCg                                                                                                                          >  5:1321281/1‑151 (MQ=255)
                         tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCAAAAAGAAATACCGAAGAATAGGG‑AAATATTTTTTAGTGATGA‑‑TA‑‑TTAATACCgg                                                                                                                         >  6:626270/1‑150 (MQ=14)
                             cggtACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCAGA‑‑tt‑‑ttttgatacctat                                                                                                                      >  2:1359923/4‑139 (MQ=11)
                             cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGctact                                                                                                                     <  1:1116721/151‑1 (MQ=255)
                              gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAGTTTACTGCTGA‑‑TA‑‑tttttaaagctaga                                                                                                                     >  1:60491/1‑138 (MQ=11)
                               aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑TATTTTGATACCGctac                                                                                                                      >  6:1823067/1‑150 (MQ=11)
                               aTACCTTCATTCCTGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTT‑‑TTGATACCGctact                                                                                                                     <  3:1819072/151‑1 (MQ=11)
                                 cccatCATTCCTCAAGATTTCGGAGAATATGCTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTCAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTaaa                                                                                                                  <  4:347910/146‑1 (MQ=14)
                                 aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTaaa                                                                                                                  <  7:539171/150‑1 (MQ=255)
                                 aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAg                                                                                                                 <  6:1631217/151‑1 (MQ=255)
                                 aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCAGA‑‑TA‑‑TTTTAGCGGCTACTaaac                                                                                                                 >  3:960536/1‑150 (MQ=14)
                                  ccTTGATTGCTTAAGATTTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGc                                                                                                                <  6:110016/151‑1 (MQ=255)
                                   catCATTGCTGAAGATTTCGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTT‑‑TTGATACCGCTACTAAAg                                                                                                                 <  7:1680663/149‑1 (MQ=11)
                                     tCATTGCTGAAGATTTCGGAGAATATGGTGCATGCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCa                                                                                                              <  8:879857/150‑1 (MQ=255)
                                     tCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TT‑‑TTGAGACCGCGCGTAAAGcgcg                                                                                                             >  3:449/1‑148 (MQ=14)
                                      aaTTTCTGAATATATAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTT‑‑TTGATACCGCTACTAAAGcc                                                                                                               <  2:792836/149‑1 (MQ=14)
                                      aaTTGCTGAAGTTTACGGAGAATATGGTGCATCCGGTAGTATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTTAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCata                                                                                                            <  2:1750167/150‑1 (MQ=255)
                                      aaTTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCata                                                                                                            <  1:771553/150‑1 (MQ=25)
                                          gCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCAGA‑‑TT‑‑TTTATATAGctaaaataaagaaataa                                                                                                         >  4:1758167/1‑136 (MQ=14)
                                           atGAATATTTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTT‑‑TTGATACCGCTACTAAAGCCGTATCa                                                                                                         <  8:1493660/150‑1 (MQ=14)
                                           atGAAGATTACGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTT‑‑TTGATACCGCTACTAAAGCCGTATCa                                                                                                         <  3:8300/150‑1 (MQ=14)
                                             gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAg                                                                                                       <  3:802016/149‑1 (MQ=255)
                                              aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTaa                                                                                                    <  8:780712/151‑1 (MQ=255)
                                                gATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAaga                                                                                                  <  5:1722905/151‑1 (MQ=255)
                                                gATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAaga                                                                                                  <  6:750183/151‑1 (MQ=255)
                                                  ttacGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatttattt                                                                                                                                                  >  5:674365/4‑105 (MQ=18)
                                                   tacGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActg                                                                                                                                             <  7:1036330/107‑1 (MQ=39)
                                                   tacGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActg                                                                                                                                             >  4:1040289/3‑109 (MQ=39)
                                                   tacGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGa                                                                                                                               <  5:260998/117‑1 (MQ=38)
                                                     cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAGAGATg                                                                                              <  5:1183093/150‑1 (MQ=40)
                                                      ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActg                                                                                                                                             >  3:1174907/1‑106 (MQ=40)
                                                                   cATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAtttt                                                                                                                                                 >  4:260278/1‑89 (MQ=37)
                                                                    aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActg                                                                                                                                             >  1:1364893/1‑92 (MQ=35)
                                                                    aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTATTTTACTGCAGA‑‑TA‑‑TTTATAAACCTAATAAAGACATATCAAGAATGAGATGTTGCGTCAGGGGGGAt                                                                              >  2:473340/1‑151 (MQ=11)
                                                                           aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa                                                                                                                                         <  2:881454/89‑1 (MQ=40)
                                                                            cgacgccacccgccGACCGCGCGGTTCGTATCCGACTACTCAATCCCGCTGAGGATCTTATGCAGCGGG‑ACTTGTATTTTCCTGTTGC‑‑TT‑‑CTGACTACGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCt                                                                      <  3:532153/139‑1 (MQ=9)
                                                                                  gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TA‑‑TTTATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCCTACGGGAGGAtaagacgcgccgagc                                                                >  1:1724868/1‑137 (MQ=11)
                                                                                  caaCCTCCGACCGCTCGGTTCGTAGCCGAGAACTCTATCCAGCTGAGCTACGTATTCATAGGG‑AAATTTATTTTACTGCTCA‑‑TA‑‑TTGATACCTCTTATATAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGAcc                                                                <  6:712446/150‑1 (MQ=17)
                                                                                     ccTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTAct                                                                                                                                              <  6:822595/74‑1 (MQ=38)
                                                                                      cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGacgcggcgagcgcgg                                                            >  3:273392/1‑137 (MQ=14)
                                                                                            ccGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                      >  1:1843566/1‑151 (MQ=255)
                                                                                             cgcgcggcgcGTAGCCGAGTTCTCCATCCAGATGAGCTACTGCTGCATCGGG‑AACTTTATATTCCTGCCCA‑‑TA‑‑TTGATACCGCGACTTCAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGt                                                     <  3:1603509/142‑1 (MQ=18)
                                                                                             cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTTATACCGCTACTAAAGCCATATCAAGAAATAGATAGTGAATCAGGGAGAATTAGACGCGCCGACCgaagcgatcgt                                                     >  6:950748/1‑141 (MQ=14)
                                                                                              gcgcggggcggaGCCGAGTACTCTATCCAGCTCCGCTACGGATGCATCGGC‑AAATTTTATTTCCTGCTGA‑‑TC‑‑TTGATACCGCTCCTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                    <  5:61875/140‑1 (MQ=17)
                                                                                              gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCCCTACTAAAGCCATATCAAGTAAGAGATCGTGCATAACGGAGGATTCTAACCACAGAcccacacgcacgtc                                                    >  8:418511/1‑139 (MQ=14)
                                                                                               cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TA‑‑TTTATACCGCTAGTAAAGCCATATCAAGTAAGAGATGGTGCAGCCGGGAGGATTATACGCGCCGAGCGATCGGTTcagac                                                   >  5:890327/1‑147 (MQ=11)
                                                                                                tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACCCTCCGACCggagcgatagtcgc                                                  >  5:569887/1‑138 (MQ=14)
                                                                                                tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAGAGATCGTGCATCAGGGAGGATTCGACGCTCCGAGCggagcgcgcgtcgc                                                  >  7:1451129/1‑138 (MQ=14)
                                                                                                 cGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatttatt                                                                                                                                                   <  8:1748510/57‑1 (MQ=39)
                                                                                                       gTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatttattt                                                                                                                                                  >  7:387048/1‑52 (MQ=40)
                                                                                                            cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGAt                                                                                                                              <  2:1297577/63‑1 (MQ=255)
                                                                                                            cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCGCCGAGCGCAGCGCTCGTCGCCGAGTagtata                                       >  7:616822/1‑145 (MQ=14)
                                                                                                            cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACGTCGTA‑‑TA‑‑TTGAt                                                                                                                              >  1:1292433/1‑63 (MQ=14)
                                                                                                               gTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATGTATTTTACTGCTGa‑‑t                                                                                                                                      >  4:98516/1‑54 (MQ=255)
                                                                                                                      aTCCAGCTGATCTTCTTACGAAGCGCG‑TAATTTATATTCCTGCTCC‑‑TC‑‑TTGCAACCGATACTAAATCAATATCTAGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                             <  6:359609/150‑1 (MQ=9)
                                                                                                                       tCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                           >  3:1448139/1‑151 (MQ=255)
                                                                                                                              gAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAAGCTCAGCCCCAACCGATCGTAGCCGAGTAGTATATCCAGCTGAGCTACCGATg                    >  1:291002/1‑151 (MQ=17)
                                                                                                                              gAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATAAAGTAAGAGATGGTGCATACGGGAGAATTCGAACCACGGAGGGATCGGAAAGTAGTGGAGTAATCTATAAAGATGAGGTAGGGagg                    >  4:838692/1‑149 (MQ=9)
                                                                                                                                   aCGGATGCATCGGG‑AAATTTATTTTACTTCTTA‑‑TA‑‑TTGATCCCGCTACTAAAGCCATATCAAGTCAGAGATGGTTCATCCGGGAGGATTCGATCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAt                 <  2:1853233/149‑1 (MQ=25)
                                                                                                                                   aCGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGTGAGGATTCGAACCTCCTATCTCTCTGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAt                 <  6:1452404/149‑1 (MQ=255)
                                                                                                                                        aggaTCGGGCAAACTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTATGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaa           <  4:538527/148‑1 (MQ=18)
                                                                                                                                         gCATCGGGAAAACTTATTCTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCCACCCTCAGACCCAACCGTTAGTAGCCGATTAATAAATCAAGATGACCTaacatcggaatagcgt           >  2:1116915/1‑135 (MQ=2)
                                                                                                                                         gCATCGGG‑AAATTTAATTTCCTGGTGA‑‑TA‑‑TTGCTACCGCTACTAACGCAATATCAAGTACGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGa            <  3:717875/148‑1 (MQ=18)
                                                                                                                                          cATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAAAAAAGGAACATCCGGGAGGATTCGAACCTACGACCGCTCGGTTAGTAGCAGAGTACTCTAACCAGCATATATAAGGATGCATCGGGAAcct        >  4:809822/1‑151 (MQ=21)
                                                                                                                                                 aaaaTTTATTTTACTGATGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAATAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttactt   <  4:1514078/149‑1 (MQ=255)
                                                                                                                                                  aaaTTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTAGGACCCCTCGCTTCGTAGCCGATTACTATATACAGCTGTCCTACCTCTGCATCGCGAAacttacata  >  1:1523050/1‑147 (MQ=18)
                                                                                                                                                     |                                                                                                                                                      
AGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TA‑‑TTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTT  >  NC_000913/2817997‑2818291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑