Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1580TA26.2% 341.0 / 116.4 217intergenic (‑23/+40)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (63/95);  new base A (19/37);  total (83/134)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

AATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTT  >  NC_000913/2818010‑2818305
                                                                                                                                                     |                                                                                                                                                     
aaTACCGCTATCTCTTCGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                                                                                                                                     <  8:549225/151‑1 (MQ=9)
 aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑t                                                                                                                                                  <  7:463090/151‑1 (MQ=255)
 aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAACTACGCAAGAGCGCTTCTTAGCCGAGTAGTCTCTCCAGATGAGCTACGTATGCCTCGGGATATTTAATTAACTGCTGa‑‑t                                                                                                                                                  >  5:1780364/1‑151 (MQ=14)
 aTTCCGCGACCTCCTCCAACCCTTCCTAGCTGAAGATCTCGGAGAATATTTTTCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑t                                                                                                                                                  <  5:1269222/151‑1 (MQ=14)
  ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGa‑‑tt                                                                                                                                                 >  1:1441959/1‑150 (MQ=255)
   tcctctagatctTCGTAACCTTCCTTGCTGAAGATTTCGGAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGa‑‑tt                                                                                                                                                 <  8:1214664/141‑2 (MQ=11)
   tCCGCTACCTCTTCGATACCTTCATGTCAGACGAGTTCAGACATGTTCGCTCCCCCGCTAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACAGCTGa‑‑ta                                                                                                                                                 <  3:106637/150‑1 (MQ=14)
    ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑tat                                                                                                                                                <  7:545425/150‑1 (MQ=255)
    ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                               <  4:415629/151‑1 (MQ=255)
    ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                               <  3:302888/151‑1 (MQ=255)
     cGCTATCTCTTCTATACCTTCATTTCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTg                                                                                                                                              <  2:231873/151‑1 (MQ=255)
      ggaacatattgcaTACCTTCATTCATGAATATTTAGTATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                                             <  2:1450288/139‑3 (MQ=11)
      gcttcatcttgcaTACCTTAATTTCTGAAGATTTAGGAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                                             <  8:1292630/139‑3 (MQ=11)
      gCTAGCTCTTCGATACGTTCATTGCTGAATATTTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGa                                                                                                                                             <  2:1240507/151‑1 (MQ=255)
      gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTAATTTAATCCTAa‑‑tat                                                                                                                                                >  6:1264574/1‑148 (MQ=255)
      gCTACCTCTTCGATACCTTCATACCTGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                                             <  4:182404/151‑3 (MQ=14)
       gttcatcttgcttACCCTCATTGATGAAGATATCGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑ttttt                                                                                                                                              <  1:1688806/137‑2 (MQ=11)
       cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAGTTTACTGCAGA‑‑TTTTTAt                                                                                                                                            >  2:1698751/1‑151 (MQ=255)
       cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGAATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAATTTACTGCTGa‑‑tat                                                                                                                                                >  8:1537872/1‑147 (MQ=255)
          ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTAAGACCGAAACGATACTAGCCGTTTACTATAAAAAGCTGACCTACCAATGCATATAGAAAATTAATTTACTGCAGagttttatagc                                                                                                                                           >  6:1710103/1‑141 (MQ=2)
          catattgtaTACGTTAATTTATGAATATTTAGTAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑ttttttgat                                                                                                                                          <  6:314768/142‑6 (MQ=11)
             ctTCGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGTGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCg                                                                                                                                        <  8:82801/149‑1 (MQ=255)
               tggATACCTTCCTTCCTCCATATCTCGTATAAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑ttttttgatac                                                                                                                                        <  5:43227/145‑8 (MQ=14)
               tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTATCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGc                                                                                                                                       <  1:1236899/148‑1 (MQ=255)
                cGATACGTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGctac                                                                                                                                    <  7:261181/150‑1 (MQ=255)
                cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGctact                                                                                                                                   <  1:1116721/151‑1 (MQ=255)
                cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCAGA‑‑ttttttgatcgcgct                                                                                                                                    >  7:1419462/1‑139 (MQ=14)
                 gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGA‑‑TTTTtatatagctcta                                                                                                                                   >  6:1039339/1‑142 (MQ=14)
                  atccgttCATTCCTGAAGATCTCGGAGCATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGctac                                                                                                                                    <  2:1331829/145‑1 (MQ=11)
                  atacgatCATTGCTGAATATATCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGct                                                                                                                                      <  7:846948/142‑1 (MQ=11)
                  aTACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGAt                                                                                                                                            <  3:1790172/140‑1 (MQ=25)
                  aTACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGAt                                                                                                                                            >  6:1794225/1‑140 (MQ=18)
                   tccggtcataaatGAATATTTCGTACTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGctact                                                                                                                                   <  3:1036661/138‑1 (MQ=14)
                     ccTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTAACTGCTGa                                                                                                                                                     >  3:1552993/1‑130 (MQ=255)
                      catCATTGCTGAAGATTTCGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAg                                                                                                                               <  7:1680663/149‑1 (MQ=11)
                      cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAATTTACTGCAGA‑‑TATTtataaaccgagaaacga                                                                                                                              >  5:1381452/1‑137 (MQ=11)
                        tCATTGCTGAAGATTTCGGAGAATATGGTGCATGCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCa                                                                                                                            <  8:879857/150‑1 (MQ=255)
                         aaTTTCTGCAGATCTAGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGcc                                                                                                                             <  2:1216610/149‑1 (MQ=14)
                         aaTTTCTGAATATATAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGcc                                                                                                                             <  2:792836/149‑1 (MQ=14)
                         aaTTGCTGAAGTTTACGGAGAATATGGTGCATCCGGTAGTATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTTAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCata                                                                                                                          <  2:1750167/150‑1 (MQ=255)
                         aaTTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCata                                                                                                                          <  1:771553/150‑1 (MQ=25)
                          atatatGAATATTTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAg                                                                                                                               <  3:1206824/142‑1 (MQ=14)
                            ttctgcataTTTAGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCgta                                                                                                                          <  4:89372/142‑1 (MQ=14)
                            tGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATc                                                                                                                        <  7:161808/150‑1 (MQ=255)
                             gCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCaa                                                                                                                      <  4:1021941/151‑1 (MQ=255)
                             gCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCaa                                                                                                                      <  5:63085/151‑1 (MQ=255)
                             gCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCAGA‑‑TTTTTATATAGctaaaataaagaaataa                                                                                                                       >  4:1758167/1‑136 (MQ=14)
                              atGAATATTTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCa                                                                                                                       <  8:1493660/150‑1 (MQ=14)
                              atGAAGATTACGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCa                                                                                                                       <  3:8300/150‑1 (MQ=14)
                                ccaGATCTCGGACAATATGGCGCAGCCGGGAGGATTCGAACCGCCGACCGCTGTGTTCGTAGCCGAGTACTCTATCCAGCTCATCTACGGATCCATCTGGAAATTTATTTTACTTCGGA‑‑TACTGATACCGCTACTAAAGCCATATCAAGTa                                                                                                                   <  3:800049/149‑1 (MQ=14)
                                   gATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAaga                                                                                                                <  6:750183/151‑1 (MQ=255)
                                   gATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAaga                                                                                                                <  5:1722905/151‑1 (MQ=255)
                                    gttctggAGAATTTGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAagag                                                                                                               <  5:151981/146‑1 (MQ=255)
                                    aTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAagag                                                                                                               <  7:1119220/151‑1 (MQ=255)
                                      tacGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGa                                                                                                                                             <  5:260998/117‑1 (MQ=38)
                                       gcGGAGAATATGGGGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACTGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATg                                                                                                            <  5:318076/150‑1 (MQ=255)
                                        cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATgg                                                                                                           <  8:359324/151‑1 (MQ=39)
                                        cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATg                                                                                                            <  5:1183093/150‑1 (MQ=40)
                                         gtataaTATGGTGCATCCGGGAGTATTCGAACCTCCGACCGCTCGGTTCGTAGCCTAGTACTCTATCCAGCTTAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGt                                                                                                          <  2:52597/147‑1 (MQ=255)
                                          gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTg                                                                                                         <  7:314952/151‑1 (MQ=39)
                                          gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGACGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTg                                                                                                         <  2:1008440/151‑1 (MQ=255)
                                             aaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCAt                                                                                                      <  5:783462/151‑1 (MQ=39)
                                                atGGTGCATCCGGGAGTATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCAAGCTGAGCTACGGATGCATCGGCAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                                    <  4:994453/150‑1 (MQ=255)
                                                          cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCTGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTTATACAGCTACTAAAGCCATATAAAGTAAGAGATGGTGAATACCCGAGGAtta                                                                                          >  1:528872/1‑149 (MQ=14)
                                                          cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTAATTTACTGCAGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATCGTGAATACGCGAGGATTct                                                                                         >  5:61733/1‑150 (MQ=11)
                                                              aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                                                                                                                                     >  1:877970/1‑89 (MQ=40)
                                                               cgacgccacccgccGACCGCGCGGTTCGTATCCGACTACTCAATCCCGCTGAGGATCTTATGCAGCGGGACTTGTATTTTCCTGTTGC‑‑TTCTGACTACGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCt                                                                                    <  3:532153/139‑1 (MQ=9)
                                                                gaTTCGAACCTCCGACCGCTCGGGTCGGAGCCGAGTACTCTATCCAGCTGCGCTACCGAGGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGTTACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTc                                                                                   <  1:120044/151‑1 (MQ=14)
                                                                   tCGAACCTGCTACCGCTCGGTTCTTAGTCTTGAACTCCAAGACGCTGATCTTCTGATGTATTGGGTTATTTATTTTACTGGAGA‑‑TATTGTTTCCCTTAATAAAGGCATATCTCGTACTAGATGGTGCATCCGGGAGGATTCGAACCTCCg                                                                                 <  6:1490806/150‑1 (MQ=255)
                                                                     gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTACTGCTGA‑‑TATTTATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCCTACGGGAGGAtaagacgcgccgagc                                                                              >  1:1724868/1‑137 (MQ=11)
                                                                     cacccTCCGCCCGCGCGGCTCGTATCCGTGGACTCAATCCAGCTCATCCCCGGTTCGAACGGGATATTTTTTTTAAGCCTTA‑‑TCGTGTTACCTCCACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGAcc                                                                              <  3:943095/148‑1 (MQ=12)
                                                                     caaCCTCCGACCGCTCGGTTCGTAGCCGAGAACTCTATCCAGCTGAGCTACGTATTCATAGGGAAATTTATTTTACTGCTCA‑‑TATTGATACCTCTTATATAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGAcc                                                                              <  6:712446/150‑1 (MQ=17)
                                                                      aaCCTCCGACCGCTCGGTTCGTAGCCGAGTTCTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTTTTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGAcc                                                                              <  3:56014/150‑1 (MQ=255)
                                                                        ccTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTATTTTACTGCTGA‑‑TATTTATACAGCTAATAAAGACATATAAATTAAGAGATGTTGAATCAGGGAGTAttaaactcgacgagccg                                                                            >  8:668671/1‑135 (MQ=11)
                                                                        ccTCAGACCGCTCGTTTCGTATCCGAGTACTCTATCCATCTCATCTTCTTATGTATCGGGTAATTTTTTTTACTGCTGA‑‑TATTGATAACGCTAATAAAGCAATATCAAGTAATATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                           <  8:1664866/151‑1 (MQ=17)
                                                                         cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGacgcggcgagcgcgg                                                                          >  3:273392/1‑137 (MQ=14)
                                                                           cgccccgcggggTGCGGAGCCGAGACCCCAAACCCGCTGCGCTAGGGCGCCAGCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                          <  1:251080/139‑1 (MQ=17)
                                                                           ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTTATACCGCTACTAAAGCCATATCAAGTAAGAGATCGTGCAACAGCGCGGAAGAGCACCTCTGACCTCTCgt                                                                        >  7:1256206/1‑150 (MQ=14)
                                                                               ccGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                                    >  1:1843566/1‑151 (MQ=255)
                                                                                cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTTATACCGCTACTAAAGCCATATCAAGAAATAGATAGTGAATCAGGGAGAATTAGACGCGCCGACCgaagcgatcgt                                                                   >  6:950748/1‑141 (MQ=14)
                                                                                            aGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAAAGCTAATAAAGCCATATCAAATAAGAGATGGTGAATCAGGGAGGATTAGACACTCCGACCGAATCGATAGTAGCCGAGTAca                                                        >  6:705102/1‑149 (MQ=14)
                                                                                              ccGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCAGCGCTCATCGCCGAGTagtaa                                                      >  4:1426211/1‑146 (MQ=17)
                                                                                               cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGAt                                                                                                                                            <  2:1297577/63‑1 (MQ=255)
                                                                                               cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACGTCGTA‑‑TATTGAt                                                                                                                                            >  1:1292433/1‑63 (MQ=14)
                                                                                                           acAGCTGTGCTACGGATGCAGCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGGCATATCAAGTACGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                                        <  3:88040/150‑1 (MQ=255)
                                                                                                             agatgcgCTACGGCTGCATCGGGAAATTTATTTTACTCCTGA‑‑TCTTGATACCGCTACTACAGCCATATCAAGTACGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                      <  3:374864/145‑1 (MQ=21)
                                                                                                               cTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCCACGCGCCGAGCGCAGCGATCGTAGCCGAGTCGTCTATCCCGCTGAGCTACggc                                    >  7:617093/1‑150 (MQ=17)
                                                                                                                agggCTACTTATGTATTGGCATATTTTTTTTACTGTATA‑‑TCTTGATACCCCTCTTTTCGGAATATCTATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGAt                                   <  6:1865902/148‑1 (MQ=2)
                                                                                                                 gAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAAGCTCAGCCCCAACCGATCGTAGCCGAGTAGTATATCCAGCTGAGCTACCGATg                                  >  1:291002/1‑151 (MQ=17)
                                                                                                                 gAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATAAAGTAAGAGATGGTGCATACGGGAGAATTCGAACCACGGAGGGATCGGAAAGTAGTGGAGTAATCTATAAAGATGAGGTAGGGagg                                  >  4:838692/1‑149 (MQ=9)
                                                                                                                           aggaTCGGGCAAACTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTATGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaa                         <  4:538527/148‑1 (MQ=18)
                                                                                                                             gtattgcgatctttattgTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAac                       <  3:1316016/140‑1 (MQ=255)
                                                                                                                             cATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAAAAAAGGAACATCCGGGAGGATTCGAACCTACGACCGCTCGGTTAGTAGCAGAGTACTCTAACCAGCATATATAAGGATGCATCGGGAAcct                      >  4:809822/1‑151 (MQ=21)
                                                                                                                                 gggATATTTATTTTCGTGGTGA‑‑TTTTGATTTCGCTACTTAAGCAATATAAATTAATATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttact                  <  6:568224/151‑1 (MQ=14)
                                                                                                                                  gtgttatttttttTACTCCAGA‑‑TTTTCCTTCCCCTACTATCTGCATATCTATTATGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttact                  <  6:1200096/146‑1 (MQ=14)
                                                                                                                                    aaaaTTTATTTTACTGATGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAATAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttactt                 <  4:1514078/149‑1 (MQ=255)
                                                                                                                                     aaaTTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCCTTCCGTTCGTCGCCGAGTAGTCTCTCAAGCTGAGCTACGGATGCAGCGGGAAtcttactta                >  3:701199/1‑149 (MQ=21)
                                                                                                                                               tACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCt                                                                                    >  4:1635729/1‑72 (MQ=25)
                                                                                                                                                  tgctgA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTACAACCTCAGACCCAACGCATAGTAGCCGAGTAATATAACCAGCTAAGCTACGGATGGATAGGGAAAAttacattaaacaataaatttt  >  6:406113/1‑138 (MQ=12)
                                                                                                                                                   tctgA‑‑TATTGCTACCGCTACTAAAGCAATATCAAGTAATAGTTGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAtttttt  <  6:1442889/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTT  >  NC_000913/2818010‑2818305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: