Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT28.4% 715.6 / inf 619intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (221/193);  new base T (77/87);  total (328/291)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-20

Only 100 of 628 total aligned reads displayed.

AGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAC  >  NC_000913/4295910‑4296210
                                                                                                                                                      |                                                                                                                                                      
  gTCGGATCAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc                                                                                                                                                      <  7:1510266/151‑1 (MQ=14)
    ccgATAAGGCGGGTAGGCCGCATCCGACAGCACCGCCCGACCCCACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt                                                                                                                                                    <  1:1162034/149‑1 (MQ=11)
       cccaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAATTTTTACTCGTATTATCAGGCCTACGCCAGACAGAGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGTATAATGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                                                                                 <  8:1271638/148‑1 (MQ=11)
       aTAAGGGGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                                                                                                                                  <  2:183844/150‑1 (MQ=14)
       aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                                                                                 <  4:351269/151‑1 (MQ=14)
         aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                                                                               >  4:1137848/1‑151 (MQ=14)
         aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                                                                               >  7:982012/1‑151 (MQ=14)
         aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                                                                                <  7:514006/150‑1 (MQ=31)
         aaGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACTCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCTTATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                                                                               <  2:1161841/151‑1 (MQ=255)
          cggCGTTAATCCCGCATCCGACAGCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                                                                              <  5:1125321/150‑1 (MQ=9)
          attcGTTTACGCTGCATCCGACATCAACGCGTGATGCTTCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                                                                              <  6:1571284/148‑1 (MQ=11)
             cGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGAGTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTACGCCGCATCCGACATCAAAGCCTGATGCGACg                                                                                                                                           >  1:964786/1‑151 (MQ=9)
              gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCGGAGAGCACCCCCTGAAGCGACgg                                                                                                                                          >  5:401317/1‑150 (MQ=11)
              gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATACGACATAACCGCCtcctgctaccc                                                                                                                                          >  1:1603198/1‑141 (MQ=11)
              gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGc                                                                                                                                          >  4:809527/1‑151 (MQ=14)
               tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCt                                                                                                                                         >  7:1322404/1‑151 (MQ=14)
               tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCt                                                                                                                                         >  1:1754758/1‑151 (MQ=14)
                ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                                                                         <  3:1198862/150‑1 (MQ=31)
                   tGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGcc                                                                                                                                     >  1:595859/1‑151 (MQ=33)
                     ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc                                                                                                                                   >  3:1509738/1‑151 (MQ=21)
                     ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgc                                                                                                                                   <  6:205786/151‑1 (MQ=35)
                     ccGAATCCGACTTAATCGACTGATGCGTCGCTTTCCTCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc                                                                                                                                   <  3:643955/151‑1 (MQ=11)
                      cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGACCGATAAGGCGTTTACGACGCATCCCACATCAACGAATGATGCGACCCGTGCcggg                                                                                                                                  >  8:473590/1‑149 (MQ=11)
                       gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                                 >  1:669058/1‑151 (MQ=21)
                        cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                                                                                >  3:1739599/1‑151 (MQ=21)
                          tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                              >  8:697989/1‑151 (MQ=21)
                           ccGACATCAACTCCTGATGCGACGCTTCCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                                                                               <  1:504789/149‑1 (MQ=255)
                           ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                              <  4:861454/150‑1 (MQ=35)
                           ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAAACCTCATGAGACCCtagacgaggcta                                                                                                                              >  6:1084761/1‑139 (MQ=11)
                             gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                           >  8:1521937/1‑151 (MQ=21)
                             gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                           >  7:1098406/1‑151 (MQ=21)
                              aCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                          <  3:984463/151‑1 (MQ=35)
                                aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                                        >  5:1387115/1‑151 (MQ=35)
                                aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCAGCGTCTTATCAgg                                                                                                                        >  2:1417955/1‑151 (MQ=18)
                                  gaaCGCCTGATGCGACGCGTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                      <  5:262261/150‑1 (MQ=21)
                                   aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                                                     >  7:276054/1‑151 (MQ=21)
                                      gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc                                                                                                                  >  3:1419469/1‑149 (MQ=18)
                                        cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                                                                                >  8:1406523/1‑147 (MQ=14)
                                          gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta                                                                                                               <  5:1535328/150‑6 (MQ=9)
                                             gctaCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATTGGCTGATTTAGCGTGATTTTGTATGTCTTATAAGGCGTTTACGCCGCATAAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa                                                                                                           <  4:157595/148‑1 (MQ=11)
                                              cGACGCTTAACGCTTCTTATCAGGCCTACGCCAGACAGCTAAATAGCTTTATTTAGCGTGATTTTGTAGGTCGTATAAGGCTTTTACTCCGCATCCTACTTCAAAGCCTGATGAGACGCTTGCCGCTTCTTATCAGGCCTACGCCAGACAg                                                                                                          <  6:466171/151‑1 (MQ=11)
                                                     tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCATGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATACGAAATCAAAGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCGCAGACAGCGCAAt                                                                                                    >  6:632854/1‑150 (MQ=11)
                                                     tAACGCGTCTTATCAGGCCTAAGAAAGACATCGCAATAGAATGATTTAGAGTGATTTTGTAGGTAGGATAAGGCGTTTAAGCCGCATCCGACATCAAAGAATTATGATAAGCTTGCCGCGTCTTATCAGGCCTAGGGGAGACGGCGCatt                                                                                                    >  2:1320101/1‑148 (MQ=2)
                                                      accgcgTCTTATCAGGCCTACGCCAGACAGAGCAATAGCCTGATTTAGCGTGATTTAGTAGCGCGGCTACGGCGTTTACGCCGCATCAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAg                                                                                                  <  7:398891/149‑1 (MQ=11)
                                                      aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAg                                                                                                  <  3:1632333/151‑1 (MQ=35)
                                                          cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACGGCGCAAGAGCCTg                                                                                              >  3:1342520/1‑151 (MQ=25)
                                                            gcTTATCAGGCCTACGCCAGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATACGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt                                                                                            <  5:1017779/150‑1 (MQ=255)
                                                             gttATCAGCCGTACGCCAGACAGAGCAATAGACTGATTTAGCGTGATTTTGTAGGTCTGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAtt                                                                                           <  3:1052959/150‑1 (MQ=255)
                                                                 tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGc                                                                                       >  1:1032834/1‑151 (MQ=25)
                                                                  cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACATCAACGCCTGATTCGACGCTTGCCGCGTCTTATCATGACTAAGACAGAAAGCGCAATAGCCTGATTTAGCg                                                                                      >  6:863506/1‑151 (MQ=11)
                                                                      ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGAAAACGGAATATACTCCTCATCCGAAATCAACTCCAGATAAGACGATATCCGCGTCTTATCAGGAATAATAAAAAAAGCGCAATAGCCTGATTTAGCGTGAt                                                                                  >  4:607785/1‑151 (MQ=2)
                                                                       cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAAGAAAGACAGCGCAATAGAATGATTTAGCTTGAtt                                                                                 >  2:926335/1‑151 (MQ=11)
                                                                       cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTATTAACAGGCATACTCCAGACAGCGAAATAGCGTGATTTatctttatt                                                                                 >  2:573093/1‑143 (MQ=11)
                                                                           gCCAGACAGCGCAATAGCCTGATTTAGCTTGATTTTTTCTGTCGGATTAGGCGTTTACGCCGAATCCGACATCAACGCCTGATGCGACTCTTGGCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                             <  3:860713/151‑1 (MQ=11)
                                                                           gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAAGCGAGACCGCGCTATAGGATGATTTATCTTGATATTGt                                                                             >  1:1656697/1‑151 (MQ=11)
                                                                           ccccgatacgccAATATCCGGTTTTAGCGTTATTGTGACGTTCGGATAAGGGCTGTACTCCGGTTCCGTCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                             <  5:616464/140‑1 (MQ=11)
                                                                             cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAAGCCAGACAGCGCAATAGCCTGATTTATCGTGATTTTGt                                                                             >  5:1640049/1‑149 (MQ=25)
                                                                              cgACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCTGATACGGCGTTTTCGGAGCATAAGAGATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAgg                                                                          <  2:757647/150‑1 (MQ=11)
                                                                               gACAGCTCAATAGCCGTATTTAGCCTGATTATGTACCGATGCTACGGCCTATACGCCGCATAATACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt                                                                         <  7:818205/151‑1 (MQ=11)
                                                                                cccggccAATACCCTGCATTAGCCTGATTTTGTCGCACGGATAAGGCGTTTAGTCCGCATCCGACATCAAAGCCTGATCCGCCGCTTGCAGCGTCTTTTCCGGCCTACGCCAGACTGGGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                        <  3:1819372/145‑1 (MQ=11)
                                                                                   gcgcTATAGCCTGTTGTCTCGTGATGTTGGCGGTGCGATACGGAGCTTACGCCGGAGCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGa                                                                     <  5:189737/151‑1 (MQ=11)
                                                                                    atcAATAGACTTATTTAGCGTGATTTTGTAGCGCGTATATGGCTTTTACTCCGCTTAAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt                                                                    <  6:998518/149‑1 (MQ=11)
                                                                                        aaaGCCTGATTTATAGTGATTGTGTACCTCTGTTTCGGTCTTTACGCCGCTTCCGACATGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAgg                                                                <  8:1137507/149‑1 (MQ=11)
                                                                                        aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAgg                                                                <  1:304670/151‑1 (MQ=25)
                                                                                           tacggcttaaGCGCGATTTTTTACGTATGATCACGAGTTTATGCCTCATCGTACATCTTCGGATGTTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCg                                                              <  8:1715154/141‑1 (MQ=2)
                                                                                           gCCTGCTGTTGCGTGTTTTTGTAGGCCGTTGTCGGCCTTTACCCCGGATACTAGATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGt                                                             <  5:993626/151‑1 (MQ=11)
                                                                                            ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAACAAAGACAGCACAAAAGCCTGATTTAGAGTGATTTTCTAGGTCGTATAAGTCGtt                                                            >  8:824194/1‑151 (MQ=11)
                                                                                               cagttaggctGATTTTGTAGGTAGGATAAGGCTTTTACGCCGGATCCTAGATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTa                                                          <  5:1800116/141‑1 (MQ=11)
                                                                                                 tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGACTACGACAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGc                                                       >  4:47810/1‑151 (MQ=255)
                                                                                                   tAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCGAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg                                                     >  5:1301394/1‑151 (MQ=25)
                                                                                                     gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGGCAGACCGCGCATTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGc                                                    >  7:454791/1‑150 (MQ=255)
                                                                                                     gCGTGATTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCAGCAt                                                  >  6:142065/1‑151 (MQ=255)
                                                                                                         gagtttGTAGGTAGGATTCGGAGTTTATGTCGCTTCCGACATGAAGGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCg                                               <  4:1844254/148‑1 (MQ=11)
                                                                                                         gATTTTGTCGCTCGTATAAGGCGTTTACTCCGCCTCCTACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATcc                                                <  1:1494651/150‑1 (MQ=11)
                                                                                                             ttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACa                                            >  2:37127/1‑150 (MQ=25)
                                                                                                                 cggTCGGATACGGCGTTTACGCCGCATACGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc                                       <  3:509360/150‑1 (MQ=25)
                                                                                                                 cgcgcgtcgccggCGTTTACCCCGCATCAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc                                       <  5:903637/140‑1 (MQ=11)
                                                                                                                  ggTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCCTCATTTTGTAGGTCCGATAAGGCCTATATGCCGCATCCGACATCAACg                                      >  1:1372944/1‑151 (MQ=255)
                                                                                                                         tAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATg                               >  5:526525/1‑151 (MQ=35)
                                                                                                                         tAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGACAGACAGCGAAATAGCCTGATTTAGCGTGATTTTCTAGGTCCGATAAGGCGTTTATGCCGCATCCGACATCAAAGCAAGATg                               >  6:778488/1‑151 (MQ=11)
                                                                                                                           ccgCGTTTCCGCCGCCTCCGACATCAACCCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCg                             <  7:1799876/149‑1 (MQ=255)
                                                                                                                           aGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCg                             >  7:1656636/1‑151 (MQ=35)
                                                                                                                            ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCg                             >  2:1222360/1‑150 (MQ=35)
                                                                                                                              cgttccctcctcATCCGACATCATCGCTTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACg                          <  2:156348/140‑1 (MQ=11)
                                                                                                                               gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCATGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAAGCCTGATGCGACGc                         >  8:185631/1‑151 (MQ=255)
                                                                                                                                 ttACGCCGCCTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCt                        <  8:588155/150‑1 (MQ=25)
                                                                                                                                  tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAAGCCTGATGCGACGCtt                       >  2:1704471/1‑150 (MQ=25)
                                                                                                                                   aCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTa                      >  7:1768429/1‑150 (MQ=35)
                                                                                                                                    cgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTCAc                    >  4:1758587/1‑151 (MQ=21)
                                                                                                                                    cgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAc                    <  3:238943/151‑1 (MQ=34)
                                                                                                                                     gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAAGCAAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAAGCCTGATGCGACGCTTAAcg                   >  8:299662/1‑151 (MQ=16)
                                                                                                                                        gcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt                >  7:1189286/1‑151 (MQ=33)
                                                                                                                                         cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt                >  4:1344305/1‑150 (MQ=33)
                                                                                                                                         cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc               >  3:367746/1‑151 (MQ=33)
                                                                                                                                          aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCt              >  5:325980/1‑151 (MQ=33)
                                                                                                                                           gcagACATCAACGCCTGATGCGACGCTTCCCGCTGCTTATCCGGCATACGCCAGAGAGCGCAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCtt             <  7:698100/148‑1 (MQ=11)
                                                                                                                                                cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGTATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGTTTAACGCGTCTTATCAg        <  6:316751/151‑1 (MQ=18)
                                                                                                                                                  ccAACGCCTGTGGCGACGCGTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc      <  5:821941/150‑1 (MQ=12)
                                                                                                                                                   cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGcc     >  6:1241680/1‑151 (MQ=32)
                                                                                                                                                      cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAc  <  1:1217502/151‑1 (MQ=11)
                                                                                                                                                      |                                                                                                                                                      
AGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAC  >  NC_000913/4295910‑4296210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: