Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,392,4570GT25.3% 411.2 / 22.0 279intergenic (+22/‑15)glyV/glyXtRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (73/104);  new base T (0/60);  total (78/201)
Fisher's exact test for biased strand distribution p-value = 5.60e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

CCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAG  >  NC_000913/4392309‑4392606
                                                                                                                                                    |                                                                                                                                                     
     aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGa                                                                                                                                                 <  6:1234845/150‑1 (MQ=255)
       cgacgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGacca                                                                                                                                              <  2:742952/151‑1 (MQ=255)
        gacgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCAAAGacaac                                                                                                                                             >  2:618028/1‑151 (MQ=255)
            cAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCaa                                                                                                                                         <  1:1032280/151‑1 (MQ=255)
                 aTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCggg                                                                                                                                    <  3:1114669/151‑1 (MQ=255)
                 aTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCggg                                                                                                                                    <  2:913067/151‑1 (MQ=255)
                 aTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGAAAAGCCCAGACCAccccacggcg                                                                                                                                    >  3:338413/1‑144 (MQ=255)
                   gCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGaa                                                                                                                                  <  3:275325/151‑1 (MQ=255)
                   gCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTAGAGCACAGCTCTGACCAccccacccggc                                                                                                                                   >  7:1827925/1‑142 (MQ=18)
                        aaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTTGGTGCAATGCACCGACCACCCAAGCGGGaagcgct                                                                                                                             >  7:471966/1‑146 (MQ=17)
                         aTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAATTGATTAAAAGCACAAACCCCCCAAgagctaagaactc                                                                                                                            >  4:904760/1‑139 (MQ=18)
                           tACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCa                                                                                                                           <  6:722542/150‑1 (MQ=255)
                             cGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGtt                                                                                                                        <  7:780887/151‑1 (MQ=255)
                             cGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGATAAAAAAATAGAAAAGTGAAGCAAAACACAGACCAACAAAGAGTAAATATCTCAGtt                                                                                                                        >  6:1671991/1‑151 (MQ=17)
                                   cAAAATTAAGTAGTACGCGGGAATACCTCAGTTGGTAGCGGACGACCTTGCCTAGGTCGGGGTCGCGAGTTCGTGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACGACCCAAGCGGGAATAGCTCAGTTGGTaga                                                                                                                  <  8:359994/151‑1 (MQ=17)
                                    aaaaTTATGTAGTACGCGGGAATAGCTAAGTTGTTAGAGGACGAGCTTGCCAAGTTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTTCTTCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTagag                                                                                                                 <  2:1524236/151‑1 (MQ=17)
                                     aaaTTAAGTAGTACGCTGGAATAGCTCAGTTGGGAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTaga                                                                                                                  <  8:1065498/149‑1 (MQ=255)
                                      aaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGGCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCa                                                                                                               <  6:863067/151‑1 (MQ=255)
                                       aTTAAGTACTACGAGGGAATATCTCAGTTGTTTGAGCACGTCCTTGCGTAGGTAGGGGTCGCTAGTTCGAGTCTCGTTTACCGCTCCTAAATTTGAAAAGTTCTGAATACAACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCAc                                                                                                              <  8:1024845/151‑1 (MQ=14)
                                              agtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTg                                                                                                       <  5:1784313/151‑1 (MQ=255)
                                              agtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGGCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCtt                                                                                                        <  1:500541/150‑1 (MQ=255)
                                                 aCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCTAGTTCGAGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTGCTGCAAATCTCAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGc                                                                                                      <  6:1348662/149‑1 (MQ=255)
                                                  cgcgGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGTGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCaa                                                                                                   <  7:1251719/151‑1 (MQ=39)
                                                  cgcgGGAATAGCTCAGTTGGTAGAGCACGACCTTCCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCaa                                                                                                   <  5:1605593/151‑1 (MQ=39)
                                                   gcgGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGCCCAACCAAGCGGGAATAGCTCAGTTGGTAGAGAACGACCTTGCCa                                                                                                    >  3:379795/1‑149 (MQ=255)
                                                   cagggAATAGCTCAGTTTGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGATTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTTCTGCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                                                                  <  8:1672085/149‑1 (MQ=255)
                                                    agggAATAGATCAGTTTGTAGAGCACGACCTTTCCAAGGTCGTGGTCGCTATATCGAGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTTCTGCAAATAACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                                                                  <  6:327207/149‑1 (MQ=14)
                                                     gggTATAGCTCTGTTGTTAGGGCAGTTGCTTCCCAATGGCGGGGTCGGCACTTGGCCCGTCGTTTCCCGCTCAAAAATTTGAAAAGTCCGCCATATCACTGACGACGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGt                                                                                                <  7:1195003/151‑1 (MQ=9)
                                                       gAATAGCTCAGTGGGTAGAGCACGACCTTGCCAAGGGCGGGGTCGCGAGTTCGAGGCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCACACAACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                              <  7:1341181/151‑1 (MQ=14)
                                                       gAATACCTCAGTTTGTAGAGCACGACCTTGCCCAGGGCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTTCTTCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                              <  2:1727309/151‑1 (MQ=14)
                                                         atcgCTCAGTTGGTCGAGCACGAGCTTGCCTAGGGCGTGGTCGCGAGGTCGAGTCTCGTTTCCGGCGCCAAAATTTGAAAAGTGCTGCAACGCACAGACCACCCAAGCGGGAATAGCTGAGTTGGTAGAGCACGACCTTGCCACGGTCggg                                                                                            <  5:670876/148‑1 (MQ=11)
                                                           aGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGTGTCGCGAGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTTCAAACCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGt                                                                                          <  3:1786174/151‑1 (MQ=255)
                                                            gataaGTTGGTAGAGGTCGACCTTGGCTATGTCGTGGTCGCGAGTTCGAGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTTCTGCAAATCACAGACCAACCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                         <  6:1394271/147‑1 (MQ=14)
                                                            gCTCAGTTTTTAGAGCCCGACCTTTCCAAGTTATGGGTCGCTATTTCGAGTCTCGTTTCCGTCTCCAAAATTTGAAAAGTCCTGCAAACAACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                         <  2:232236/151‑1 (MQ=14)
                                                            ccTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                         <  7:855267/150‑1 (MQ=38)
                                                             gtCAGTTTGTAGCGGAGGTGCTTGCCTATGTCTGGGTCGGTTGTTGGTGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTGCTGCAACTCAAAGACGAACCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                         <  2:30064/149‑1 (MQ=2)
                                                             atCAGTTGTTTGAGGTCGTCCTTCCGTAGTTAGGTGTCGCGTCATCGAGTGTCCGTTCCCGCTCCTAAATTTGAAAATTGCTGAACATCACAGACGAAGCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                         <  4:1286032/149‑1 (MQ=2)
                                                               cAGTTGGTAGCGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc                                                                                       <  7:1535145/150‑1 (MQ=38)
                                                               cAGTTGGTAGAGCTCGACCTTCCGAAGGGCGGTGTCGCGAGTTCGAGTCTCGTTTCCCGCGCCAAAATTTGAAAAGTGCTGCAAATAACAGACCACCGAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCTAGGTCGGGGTcg                                                                                        <  4:350686/149‑1 (MQ=14)
                                                                aGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTTCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa                                                                                     <  6:1568728/151‑1 (MQ=38)
                                                                 gTTTGTAGTGCACTAGCTTGCCAAACTCGGGGTCGCGAGTTCGAGTCTCCTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAACCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAg                                                                                    <  5:1549984/151‑1 (MQ=14)
                                                                    ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                                                                 <  1:836528/151‑1 (MQ=38)
                                                                    ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCGCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                                                                 <  4:1514567/151‑1 (MQ=38)
                                                                     gTAGTGCACGACCTTGCCAATGTAGTGGTCGCGTGTTCGAGTCTCGTTACCCTCTCCAAAATTTGAAAAGTCCTTCAAACCACAGACCACGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                                                                 <  7:1732701/150‑1 (MQ=14)
                                                                     gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                                                                 <  7:1033138/150‑1 (MQ=255)
                                                                       agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                               <  5:717643/150‑1 (MQ=38)
                                                                       agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTCGTGAATAGCCCAAACAACCCAAGCGGCAAGCGCTCAGGTGCTAGACCACGACCTTCCCAAGGAGGCGGTCGGCTGTTCGat                                                                              >  7:623601/1‑150 (MQ=2)
                                                                          gCAGGACCTTACCAAGGTCGTGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTTCTGAAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                           <  3:50086/151‑1 (MQ=255)
                                                                           gaCGAGCTTGCCTAGGTCGGTGTCGCTAGTTCGTGGGTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACTGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc                                                                          <  4:628852/150‑1 (MQ=14)
                                                                           cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGATTAAAACCACAGACCACCCAACCGGAAAAACCACATTTGGTAGAGAACGCAATAGCCAAGGTGTGGGTAACGAGTTCGAGtct                                                                           >  4:1167112/1‑150 (MQ=11)
                                                                           cACGACCTTCCCCAGCTCGGGGTCGCGAGATCGCGTCTCGGTTCCCGCTCAAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc                                                                          <  7:267573/151‑1 (MQ=14)
                                                                            actaCCTTGGCAAGGTCGGGGTCGTGATTTCGACGCTCGTTTCCCTCTCCAAAATTTGAAAATTCCTCCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCg                                                                         <  2:1080607/148‑1 (MQ=14)
                                                                              gACCTTGCGAAGGTCGGGGTCGCGAGTTCGAGCCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTCCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtt                                                                       <  7:644585/151‑1 (MQ=255)
                                                                              gACCTTGCCAAGGTCGGGGTCGCTAGTTCGAGTCTCGTTTCCCGCTCCTAAATTTGAAAAGTGCTGCAAAGCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtt                                                                       <  2:460575/151‑1 (MQ=255)
                                                                               aCCTTGCCAAGGTCGGTGTCGCTAGTTCGAGGCTCGTTTCCCGCTCAAAAATTGGAAAAGTCCTCCACAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGttt                                                                      <  5:1055160/151‑1 (MQ=14)
                                                                                ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTTCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                     <  6:381167/151‑1 (MQ=38)
                                                                                   tcccAAGGGCGGGGTCGCGAGTTCGAGTTTCGTTTCCCTCTCCAAAATTTGAAAAGTGCTTCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTcc                                                                    <  6:181136/147‑1 (MQ=255)
                                                                                   tGCCAAGGTCGTGGTCGCGAGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTTCAAATCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                                   <  3:13618/150‑1 (MQ=255)
                                                                                    gCCAAGGTCGGGGTTGCTAGTTCGAGTCTGGTTTACAGATCCATAATTTGAAGAGTTCTCCAACCAAAATAACTCGCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCg                                                                  <  3:1668247/150‑1 (MQ=11)
                                                                                          ggcGGGGTCGCGAGTTCGCGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTCCTCCACATCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaa                                                           <  5:1369824/149‑1 (MQ=14)
                                                                                            cGGGGTCGCCAGATCCAGGCGCGGTACCCGCTCAAAAATTAGAAAAGTCCGCCAAACCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAt                                                         <  1:1269298/151‑1 (MQ=14)
                                                                                            agttgtcggtaGTTCGAGGGTCGTTTCCGGCTCATAAATTTGAAAAGTCCTGAACATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaaa                                                          <  2:858257/140‑1 (MQ=11)
                                                                                               tgtcgttatttCGAGTCTCGTTTACCGCTCAAAAATTTGAAAAGTGCTTAAAATAACATACCACGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAttt                                                       <  8:756590/141‑1 (MQ=14)
                                                                                                gtcggtatttcggctctcGTTTCCCCCGCAAAAATTTGAAAAGTGCGACAACACACTGACCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTg                                                      <  1:2854/136‑1 (MQ=14)
                                                                                                 tCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGAAAAGCACAGCCCACCCAACCGGCAAAAGCTCAGTTGGTAGAGCACCACCTTGCCATGGTCGGGGTCGAGAGGTCGAGTCTCGTTTACCGATCCAAATTTTGat                                                    >  5:533283/1‑150 (MQ=11)
                                                                                                  cgggAGTTGGACTCTCCTTTCCCTCTCCAAAATTTGAAAAGTCCTGCAAATCACAGACGACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaa                                                    <  5:97929/147‑1 (MQ=14)
                                                                                                        atCGAGTGTCGTTTCCCGCTCCTAAATTTGAAAAGTGCTGCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCt                                              <  3:682088/149‑1 (MQ=255)
                                                                                                              cctcGTTACCCGCTCAAAAATTAGAAAATTCCTCCACCAAACAGAACAACCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGc                                       <  1:701641/150‑1 (MQ=14)
                                                                                                               cgcGTTACCCCCTCAAAAATTTGAAAATTTCTGAATATAAAATACGAACCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGca                                      <  8:1556545/149‑1 (MQ=14)
                                                                                                                 cgttaaccGCTCCAAAATTAGAAAAGTCCTCCAAATCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGca                                      <  3:853226/143‑1 (MQ=17)
                                                                                                                 cGTTTCCCGCTCAAAAATTTGAAAATTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGca                                      <  3:984559/149‑1 (MQ=255)
                                                                                                                  ggttCCCTCTCCAAAATTTGAAAAGTGCTGCAAACCACAGACGACGGAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAg                                   <  8:179195/149‑1 (MQ=18)
                                                                                                                  gTTTCCCGCTCAAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAg                                   <  1:614270/151‑1 (MQ=255)
                                                                                                                   gttCGCGCGCAAAAATTTGAAATGTTCGGAATCCAACAGACCACGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCTAGGTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGa                                  <  4:798803/150‑1 (MQ=14)
                                                                                                                     tCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAAATTGCTGTaattgccagagc                                >  5:133949/1‑141 (MQ=255)
                                                                                                                       cgccTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACGAACCAAGCGGGAATAGCTCAGTTTGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGaccac                              <  7:362141/148‑1 (MQ=255)
                                                                                                                          cTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTGATATAGATGTAATGTCCAGaccgaccc                           >  1:952448/1‑146 (MQ=14)
                                                                                                                           tCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGGCAATGAAAAGCTCGGTGTAGCGAGTTAGAGTCACGATTACGGATCAAAAATTTGGAAGTGCTTTAATGAACAGAAAACCCac                          >  4:500370/1‑150 (MQ=12)
                                                                                                                            ccAAAATTTGAAAATTCCTTCAACTCACATACCTACCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCaa                          <  6:605327/150‑1 (MQ=17)
                                                                                                                            ccAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGATCGGGCTGTAATGCACAGAcccccccgg                         >  5:258986/1‑148 (MQ=17)
                                                                                                                            ccAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGATAGTGCTGTAAGGCCCAGAGCccccccg                         >  1:432133/1‑148 (MQ=255)
                                                                                                                             ataaaTTTGAAAATTGCTGCATATCAATTAACACCTAAGCTGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGAGCCAAAATTTGAAAGTGCGGTAAGGCACAGACCACCCAAGc                        <  6:1070955/149‑1 (MQ=14)
                                                                                                                              aaaaTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACCCCCTTGCCAACGTCGGGCTCGCGAGTTCGCGGCGCGTTTCCCGGTGCAATATTTGAAAGTGATTTAAGGCACAGgccccgcccgcg                       >  5:1190149/1‑142 (MQ=17)
                                                                                                                                aaTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATAAAAGAACTGTAACGCCCAGaccaacacagaggc                     >  8:328632/1‑141 (MQ=14)
                                                                                                                                 aTTTGAAAATTCCTCCAAATCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGa                    <  5:1224068/151‑1 (MQ=255)
                                                                                                                                  tttGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTCGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGAAATATAAGGACCACACCAACCAAGCGGGat                   >  5:1480368/1‑150 (MQ=17)
                                                                                                                                  tttGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTGATAGTGATGTAATGACCAGACCCCCCACGCGGGat                   >  7:226461/1‑150 (MQ=17)
                                                                                                                                    ttaaaaGTCCTGAACAGCACAGACCACGCAAGCGGGAATAGCTCAGATGGTAGAGCACGACCTTGCCATGGTCGGGGTCGGGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAAt                  <  2:1571268/148‑1 (MQ=17)
                                                                                                                                    tGAAAATTGCTTCATATAACTTACGACGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCATGGTGGGGGTCGCGAGTTCGAGTCTGGTTTCCCGCTCCAAATTTTGAAAGTGCTGTATGGCACAGACCACCCAAGCGGGAAt                  <  6:809934/150‑1 (MQ=14)
                                                                                                                                     gAAATGTGCTCCAAAGAACATACCACGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATa                 <  2:1341213/150‑1 (MQ=14)
                                                                                                                                     gAAAATTGCTGAAACACACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAg                <  3:536599/151‑1 (MQ=255)
                                                                                                                                       aaaGTCCTGCAACCCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCt              <  3:1692057/151‑1 (MQ=255)
                                                                                                                                           ttctccAAATCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGGCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAACGCACAGACCACCCAAGCGGGAATAGCTCAg           <  8:438507/145‑1 (MQ=14)
                                                                                                                                           tgctgcAAAGCACAGACCAGCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGATATTCATGAAAGGACCAGAGCCCCCCAGGGGGATTAGGGCAGt          >  5:1874720/1‑151 (MQ=14)
                                                                                                                                           tgctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGAGCTCTAAGGAACACACCACCCAAGAGGGAATAGCTCAGt          >  6:245515/1‑151 (MQ=255)
                                                                                                                                             ctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGACTTCGAGTCTCGTTTCCCGCTACAAAATTTTATATTTATAAAATGCAAAAAACACCCCAGCGGGAATAGCTCTGttt        >  6:1159707/1‑150 (MQ=14)
                                                                                                                                              tgcAAAGCACAGCCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATGAAATAGCTATAAGACACAGACCGCCCAACCGCGAAAAGCTAAGtttt       >  2:95623/1‑149 (MQ=17)
                                                                                                                                              tgcAAAGCACACACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGACTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAGAGATAAAAGAGACACACCGCCCCAGCGCGAATAGCACCGTTgt       >  3:1227449/1‑150 (MQ=14)
                                                                                                                                                  aaGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTTAAATTGCTTTAATGAACACACCACCCCACAGGGATTATCTAAGTTgttttt   >  1:1392067/1‑146 (MQ=14)
                                                                                                                                                   aGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGCTCGTCGTCGCGACATGGAGTCGGGATTAGCGCTCAAAAATTTTAAAGTTTTATAAAGCACAGACCCCCCACGCGGGAATAGCTCAggtggtggcg  >  7:15551/1‑147 (MQ=9)
                                                                                                                                                    |                                                                                                                                                     
CCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAG  >  NC_000913/4392309‑4392606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑