Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 962,358 G→C V122L (GTT→CTT)  rpsA → 30S ribosomal subunit protein S1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913962,3580GC98.0% 1877.9 / 9.6 490V122L (GTT→CTT) rpsA30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand):  ref base G (3/7);  new base C (234/245);  total (237/253)
Fisher's exact test for biased strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 490 total aligned reads displayed.

TGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTC  >  NC_000913/962209‑962505
                                                                                                                                                     |                                                                                                                                                   
 ggACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCtt                                                                                                                                                   <  1:1436643/151‑1 (MQ=255)
  gACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTTGATCACGCTGGAAAAAGCTTACGAAGATGCTTAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTg                                                                                                                                                  <  2:832074/151‑1 (MQ=255)
  gACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTg                                                                                                                                                  <  8:1468891/151‑1 (MQ=255)
  gACGCAGTAGAAGACGGCGTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGGCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTg                                                                                                                                                  <  2:936301/151‑1 (MQ=255)
   aCGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGa                                                                                                                                                 <  2:975770/151‑1 (MQ=255)
   aCGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGa                                                                                                                                                 <  4:1748711/151‑1 (MQ=255)
     gCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGc                                                                                                                                               >  4:1391789/1‑151 (MQ=255)
       aGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGGAAAGTTAAGGGCGGCTTCACTCTTGAGCTg                                                                                                                                             >  2:480619/1‑151 (MQ=255)
         tAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGa                                                                                                                                            >  3:1617663/1‑150 (MQ=255)
           gaagaCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACg                                                                                                                                         >  2:105438/1‑151 (MQ=255)
             agaCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGt                                                                                                                                       >  8:396792/1‑151 (MQ=255)
                cGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTAtt                                                                                                                                    <  1:1219352/151‑1 (MQ=255)
                 ggCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTc                                                                                                                                   <  2:60091/151‑1 (MQ=255)
                 ggCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTc                                                                                                                                   <  7:1315662/151‑1 (MQ=255)
                  gCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGCCGGCTTCACTCTTGAGCTGAACGGTATTCg                                                                                                                                  >  7:49721/1‑151 (MQ=255)
                     tCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTg                                                                                                                                <  8:1788021/150‑1 (MQ=255)
                     tCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTg                                                                                                                                <  1:532584/150‑1 (MQ=255)
                      cGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGc                                                                                                                               <  5:651264/150‑1 (MQ=255)
                          gAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGATGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTc                                                                                                                           >  1:1608451/1‑150 (MQ=255)
                              ctctGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGcc                                                                                                                      >  8:581515/1‑151 (MQ=255)
                                  gctgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAgg                                                                                                                   <  3:1218807/150‑1 (MQ=255)
                                    tgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTGCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTtc                                                                                                                >  8:173721/1‑151 (MQ=255)
                                     gTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGtt                                                                                                                 <  6:1478641/149‑1 (MQ=255)
                                     gTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGtt                                                                                                                 <  5:971284/149‑1 (MQ=255)
                                     gTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTtct                                                                                                               <  2:1693513/151‑1 (MQ=255)
                                          gTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGt                                                                                                          >  3:1866761/1‑151 (MQ=255)
                                            gagaAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGt                                                                                                          >  8:47809/1‑149 (MQ=255)
                                            gagaAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTa                                                                                                         <  1:631229/150‑1 (MQ=255)
                                             agaAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGt                                                                                                          >  6:128256/1‑148 (MQ=255)
                                             agaAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGa                                                                                                       <  5:1087324/151‑1 (MQ=255)
                                               aaaGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACg                                                                                                     >  7:1446023/1‑151 (MQ=255)
                                                aaGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGt                                                                                                    >  7:878943/1‑151 (MQ=255)
                                                 aGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGtt                                                                                                   <  2:303934/151‑1 (MQ=255)
                                                  gCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTc                                                                                                  <  6:364858/151‑1 (MQ=255)
                                                         gTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGt                                                                                           >  1:398020/1‑151 (MQ=255)
                                                          tCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGGCAGGTTCTCTGGTAGACGTTCGTCCGGTg                                                                                          >  6:1328573/1‑151 (MQ=255)
                                                              gAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTg                                                                                      <  8:1040036/151‑1 (MQ=255)
                                                              gAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTg                                                                                      <  3:1762842/151‑1 (MQ=255)
                                                                 gCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGCCCGGTGCGTGac                                                                                    >  1:1054986/1‑150 (MQ=255)
                                                                     ggATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACActct                                                                               <  3:1506534/151‑1 (MQ=255)
                                                                      gATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTg                                                                              <  5:217069/151‑1 (MQ=255)
                                                                        tCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGc                                                                             >  8:1307295/1‑150 (MQ=255)
                                                                         cACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCa                                                                            <  7:1132565/150‑1 (MQ=255)
                                                                             cTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTgg                                                                       >  6:1161977/1‑151 (MQ=255)
                                                                              tGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGAGACTCTGCACCTgg                                                                       >  2:1838957/1‑150 (MQ=255)
                                                                               ggAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTgg                                                                       <  8:1606397/149‑1 (MQ=255)
                                                                                gAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAg                                                                    <  5:1569885/151‑1 (MQ=255)
                                                                                gAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAg                                                                    <  8:1225296/151‑1 (MQ=255)
                                                                                gAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAg                                                                    <  7:1847581/151‑1 (MQ=255)
                                                                                   aaaGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGc                                                                  >  3:768736/1‑150 (MQ=255)
                                                                                   aaaGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACACTTGAGCTGAACGGGATTCGTGCGTTCCGGCCAGGTTCTCTGGAAGACGTTCGTCCGGTGCGTGACACTCTGCACCTCGAACGc                                                                  >  7:1063139/1‑150 (MQ=255)
                                                                                   aaaGCTTACGAAGAGGCTGAAACTGTTACCGGTGTTAGCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCa                                                                 <  4:1141180/151‑1 (MQ=255)
                                                                                    aaGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCaa                                                                <  6:987452/151‑1 (MQ=255)
                                                                                        ttACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAag                                                              >  5:1510948/1‑149 (MQ=255)
                                                                                        ttACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAag                                                              >  8:1886303/1‑149 (MQ=255)
                                                                                         tACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAagag                                                            <  7:620313/150‑1 (MQ=255)
                                                                                         tACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAaga                                                             <  4:374218/149‑1 (MQ=255)
                                                                                            gAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTg                                                        >  5:53016/1‑151 (MQ=255)
                                                                                             aaGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGa                                                       <  4:1468123/151‑1 (MQ=255)
                                                                                                  gCTGAACCTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGGGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAAttt                                                   <  7:823691/150‑1 (MQ=255)
                                                                                                  gCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTa                                                  <  7:1759412/151‑1 (MQ=255)
                                                                                                    tGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTaa                                                 >  3:1353983/1‑150 (MQ=255)
                                                                                                     gAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTaaa                                                <  3:570043/150‑1 (MQ=255)
                                                                                                      aaaCTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGt                                              >  6:579783/1‑151 (MQ=255)
                                                                                                        aCTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTaa                                            <  7:713462/151‑1 (MQ=255)
                                                                                                         cTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAAt                                           >  2:1504920/1‑151 (MQ=255)
                                                                                                           gTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATc                                          >  1:890334/1‑150 (MQ=255)
                                                                                                             tACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCaa                                        <  1:1355277/150‑1 (MQ=255)
                                                                                                             tACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCaa                                        <  3:908743/150‑1 (MQ=255)
                                                                                                              aCCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAg                                       <  5:177379/150‑1 (MQ=255)
                                                                                                               ccGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCt                                     <  5:1362294/151‑1 (MQ=255)
                                                                                                               ccGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCt                                     <  1:1013536/151‑1 (MQ=255)
                                                                                                                cGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTg                                    >  7:1587109/1‑151 (MQ=255)
                                                                                                                      tATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATc                                >  1:1820644/1‑149 (MQ=255)
                                                                                                                       aTCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATc                                >  3:1407464/1‑148 (MQ=255)
                                                                                                                             ggCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGca                       <  4:880333/151‑1 (MQ=255)
                                                                                                                               cAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGcaac                     <  4:1287066/151‑1 (MQ=255)
                                                                                                                               cAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGcaac                     >  2:1229842/1‑151 (MQ=255)
                                                                                                                                aaaTTTAAGTGCGGATTCACTCTTGAGCTGAAATGTTTTAGTGCGTTCCTTCCAGGTTCTATGGTAGACGTTCGTCCGGGGCGTGAGACTCTTCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGcaaca                    <  7:35470/151‑1 (MQ=255)
                                                                                                                                  aGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCaacaac                  >  5:782664/1‑151 (MQ=255)
                                                                                                                                  aGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCaacaac                  <  5:1355826/151‑1 (MQ=255)
                                                                                                                                   gTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACg                 >  5:447309/1‑151 (MQ=255)
                                                                                                                                     tAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgt                <  4:707134/150‑1 (MQ=255)
                                                                                                                                      aaGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttg              <  4:485545/151‑1 (MQ=255)
                                                                                                                                       aGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgt             <  7:124816/151‑1 (MQ=255)
                                                                                                                                       aGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgt             >  4:1614745/1‑151 (MQ=255)
                                                                                                                                       aGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgt             <  5:1171621/151‑1 (MQ=255)
                                                                                                                                        gggCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgtt            >  6:1840459/1‑151 (MQ=255)
                                                                                                                                        gggCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgtt            >  4:187893/1‑151 (MQ=255)
                                                                                                                                         ggcggcTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgttg           >  3:338603/1‑151 (MQ=255)
                                                                                                                                          gcggcTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgttgt          <  8:766938/151‑1 (MQ=255)
                                                                                                                                           cggcTTCACTCTTGAGCTGAGCGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgttgtt         <  7:1700385/151‑1 (MQ=255)
                                                                                                                                            ggcTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGttt        >  4:635935/1‑151 (MQ=255)
                                                                                                                                            ggcTTCACTCTTGAGCTGAACGGTATTCGTGCGGTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGttt        <  8:1652679/151‑1 (MQ=255)
                                                                                                                                             gcTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtc       >  6:1699756/1‑151 (MQ=255)
                                                                                                                                                tCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct      <  6:222022/149‑1 (MQ=255)
                                                                                                                                                 cACTCTTGAGCTGAACGGTATTCGTCCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCtcg    <  4:315998/150‑1 (MQ=255)
                                                                                                                                                  aCTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCtcgtc  <  1:304061/151‑1 (MQ=255)
                                                                                                                                                  aCTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTGCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGAGCAACACCGTTGTTGTTTCtcgtc  <  2:1295041/151‑1 (MQ=255)
                                                                                                                                                  aCTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGATCTCTGGTAGACGTTCGTCCGCTGCGTGACACTCTGCACATGGAAGGCAAACAGCTTGAATTTAAACTAATCAATCTGGATCAGAAGCGAAACAACGTTGTTGTTTCtcgtc  >  7:992988/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTC  >  NC_000913/962209‑962505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑