Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,037,1010CA48.0% 230.4 / inf 467R351L (CGA→CTA) yedVputative sensory kinase in two‑component regulatory system with YedW
Reads supporting (aligned to +/- strand):  ref base C (134/109);  new base A (114/110);  total (248/219)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

Only 100 of 476 total aligned reads displayed.

CGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGT  >  NC_000913/2036951‑2037251
                                                                                                                                                      |                                                                                                                                                      
    agagTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTg                                                                                                                                                    <  8:1601107/151‑1 (MQ=255)
    agagTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTg                                                                                                                                                    <  3:554361/151‑1 (MQ=255)
      agTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTgt                                                                                                                                                   >  3:241364/1‑150 (MQ=255)
       gTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTgta                                                                                                                                                  <  7:855708/150‑1 (MQ=255)
         ttttCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTgtagt                                                                                                                                                <  7:1612908/150‑1 (MQ=255)
            tCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTaaa                                                                                                                                             <  1:577991/150‑1 (MQ=255)
             cAGGCTCATTAATTTTCGTTGCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGGGAATATCTAATTGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAAt                                                                                                                                           <  4:346448/151‑1 (MQ=255)
                gCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAAtttt                                                                                                                                        >  5:52084/1‑151 (MQ=255)
                gCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAAtttt                                                                                                                                        >  8:1328403/1‑151 (MQ=255)
                gCTCAATAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGGTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAAtttt                                                                                                                                        <  4:1517708/151‑1 (MQ=255)
                  tCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTAt                                                                                                                                      >  7:790600/1‑151 (MQ=255)
                      tAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCg                                                                                                                                   <  6:1491129/150‑1 (MQ=255)
                       aaTTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCa                                                                                                                                 <  1:415058/151‑1 (MQ=255)
                         ttttCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCaa                                                                                                                                >  1:138542/1‑150 (MQ=255)
                          tttCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCaa                                                                                                                                >  1:682839/1‑149 (MQ=255)
                           ttCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAg                                                                                                                              <  6:881240/150‑1 (MQ=255)
                           ttCATTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCaa                                                                                                                                >  5:8498/1‑148 (MQ=255)
                            tCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGa                                                                                                                             <  3:9369/150‑1 (MQ=255)
                               ttCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGCTATCAACAAAACTGGATATATGAAAACGCGATTTATCTGGCGAATATCAAATAGAATTAACAATAAGATAAGATAAAATTAGATGAAATAAAATTTTAtaatcaaaaaatt                                                                                                                          >  6:270351/1‑138 (MQ=255)
                                 ccAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCt                                                                                                                       <  7:309292/151‑1 (MQ=255)
                                 ccAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCt                                                                                                                       <  2:392264/151‑1 (MQ=255)
                                 agaGGGCTGGCGATAACAATATTAATCTAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGc                                                                                                                        <  6:797143/148‑1 (MQ=255)
                                    gggCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGAt                                                                                                                    <  2:746733/151‑1 (MQ=255)
                                     ggCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGAtt                                                                                                                   <  8:422683/151‑1 (MQ=255)
                                      gCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTg                                                                                                                  <  6:987114/151‑1 (MQ=255)
                                         ggCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCAc                                                                                                               >  4:744320/1‑151 (MQ=255)
                                          gCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACt                                                                                                              <  3:326052/151‑1 (MQ=255)
                                           cGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTc                                                                                                             <  5:407437/151‑1 (MQ=255)
                                           cGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTc                                                                                                             >  4:1194331/1‑151 (MQ=255)
                                            gATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCg                                                                                                            >  4:610019/1‑151 (MQ=255)
                                             atatCAATATTAAGATATCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGa                                                                                                           <  3:1599943/151‑1 (MQ=255)
                                                     atTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTaaac                                                                                                   >  8:1251268/1‑150 (MQ=255)
                                                     atTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTaa                                                                                                     >  2:1171167/1‑149 (MQ=255)
                                                      ttAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCAGCAAAGATTTGCTGATTGCAATCGACATTAAAGc                                                                                                  >  1:1572654/1‑151 (MQ=255)
                                                       tAAGATAGCTGTTGGTATCAAGAAAACTTGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGc                                                                                                  <  2:30173/150‑1 (MQ=255)
                                                       tAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAATATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGc                                                                                                  <  6:1323657/150‑1 (MQ=255)
                                                       tAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGc                                                                                                  >  7:1450393/1‑150 (MQ=255)
                                                        aaGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCa                                                                                                 >  2:263686/1‑150 (MQ=255)
                                                        aaGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCa                                                                                                 >  8:1406807/1‑150 (MQ=255)
                                                          gATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATTGCATTAACAATAAGATTCTATAACATTAGTTTTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAAt                                                                                              <  2:1281914/151‑1 (MQ=255)
                                                              gCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATctct                                                                                          <  4:40335/151‑1 (MQ=255)
                                                                 gTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCtt                                                                                         <  3:406708/149‑1 (MQ=255)
                                                                  ttGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTtct                                                                                      >  3:829044/1‑151 (MQ=255)
                                                                   tGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTact                                                                                      <  8:620995/150‑1 (MQ=255)
                                                                    ggTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACGTTAAAGCAAATCTCTTTCTCg                                                                                    >  3:613012/1‑151 (MQ=255)
                                                                    ggTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTtctc                                                                                     <  7:982078/150‑1 (MQ=255)
                                                                    ggTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTtctc                                                                                     <  5:1403669/150‑1 (MQ=255)
                                                                      tATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTc                                                                                  >  5:1508738/1‑151 (MQ=255)
                                                                        tCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTg                                                                                >  3:1043368/1‑151 (MQ=255)
                                                                         cAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGa                                                                               <  7:1153814/151‑1 (MQ=255)
                                                                         cAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGa                                                                               <  3:97207/151‑1 (MQ=255)
                                                                          aaGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGa                                                                               >  2:1006687/1‑150 (MQ=255)
                                                                          aaGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGa                                                                               >  6:665039/1‑150 (MQ=255)
                                                                             aaaaGTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGTCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAgg                                                                           <  5:994636/151‑1 (MQ=255)
                                                                             aaaaCTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAg                                                                            <  1:1270719/150‑1 (MQ=255)
                                                                              aaaCTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGt                                                                          <  3:1347638/151‑1 (MQ=255)
                                                                                aCTGTTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTAt                                                                        <  1:777833/151‑1 (MQ=255)
                                                                                aCTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTAt                                                                        >  5:337571/1‑151 (MQ=255)
                                                                                  tGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTc                                                                      >  2:1555605/1‑151 (MQ=255)
                                                                                   ggTTATTTGAATACGCGATTTTTCTGGGGAATATCTAATGGCATTTACAATATGATTCGATAACATTCGTTGTAGTATAATTTTATCCGCAAAGTTTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTAttg                                                                      <  5:1008383/150‑2 (MQ=255)
                                                                                   ggTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTc                                                                      >  1:10639/1‑150 (MQ=255)
                                                                                   ggTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTc                                                                      >  2:1432910/1‑150 (MQ=255)
                                                                                    gTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCa                                                                     >  5:634654/1‑150 (MQ=255)
                                                                                       atatGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCa                                                                     >  3:1206474/1‑147 (MQ=255)
                                                                                            aaTACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTcc                                                            <  3:1543105/151‑1 (MQ=255)
                                                                                             aTACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTcc                                                            >  5:1353338/1‑150 (MQ=255)
                                                                                               aCGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCcaa                                                          <  8:530782/150‑1 (MQ=255)
                                                                                                cgcgATTTTTCTGTCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCcaaca                                                        <  3:902823/151‑1 (MQ=255)
                                                                                                  cgATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCcaacaa                                                       >  2:334346/1‑150 (MQ=255)
                                                                                                   gATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAAt                                                     >  2:849897/1‑151 (MQ=255)
                                                                                                    aTTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAAtt                                                    >  8:340630/1‑151 (MQ=255)
                                                                                                            ggCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACtt                                            >  8:230332/1‑151 (MQ=255)
                                                                                                                  tatCAAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACCCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTAt                                      >  2:984571/1‑151 (MQ=255)
                                                                                                                   atCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTAt                                      <  1:1168851/150‑1 (MQ=255)
                                                                                                                   atCTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTAtt                                     >  7:1270747/1‑151 (MQ=255)
                                                                                                                     cTAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAACCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTg                                    >  2:488936/1‑150 (MQ=255)
                                                                                                                      tAATGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTg                                    >  1:951276/1‑149 (MQ=255)
                                                                                                                      tAATAGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTg                                    >  8:1619630/1‑149 (MQ=255)
                                                                                                                       aaTGGCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTAtt                                     <  1:241094/147‑1 (MQ=255)
                                                                                                                          ggCATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGaa                              <  4:466693/151‑1 (MQ=255)
                                                                                                                            cATTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGaa                              >  4:1575470/1‑149 (MQ=255)
                                                                                                                             aTTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGc                           <  7:467268/151‑1 (MQ=255)
                                                                                                                             aTTAACAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGc                           >  5:190670/1‑151 (MQ=255)
                                                                                                                               tAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCg                          >  8:1054868/1‑150 (MQ=255)
                                                                                                                                aaCAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGa                         <  7:373608/150‑1 (MQ=255)
                                                                                                                                aaCAATAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAg                        <  6:198590/151‑1 (MQ=255)
                                                                                                                                   aaTAAGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTcc                     >  8:929158/1‑151 (MQ=255)
                                                                                                                                    aTAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCa                    <  8:1380450/151‑1 (MQ=255)
                                                                                                                                      aaGATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGt                  <  6:1423621/151‑1 (MQ=255)
                                                                                                                                       aGATTCTATAAGATTCGTTGTAGTAAAATTTTATCCTCAAAGATTTGATGATTGCACTCGACCTTAAAGCAAATCTCTTTCTGGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCTACTTCCTTATTGAGAGAAAGCGAGTCCAGtt                 <  4:270632/151‑1 (MQ=255)
                                                                                                                                        gATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGttt                >  2:572497/1‑151 (MQ=255)
                                                                                                                                        gATTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGttt                <  8:779478/151‑1 (MQ=255)
                                                                                                                                         aTTCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTc               <  4:893921/151‑1 (MQ=255)
                                                                                                                                          ttCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTc               >  2:662776/1‑150 (MQ=255)
                                                                                                                                           tCGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCAc             >  4:130541/1‑151 (MQ=255)
                                                                                                                                            cGATAACATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCAcc            <  4:145124/151‑1 (MQ=255)
                                                                                                                                                aaCATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCaaa         <  7:627954/150‑1 (MQ=255)
                                                                                                                                                 aCATTAGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAAc       >  6:1134551/1‑151 (MQ=255)
                                                                                                                                                     tCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTCAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACAtt    <  7:242228/150‑1 (MQ=255)
                                                                                                                                                      cGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGt  <  5:1055151/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGT  >  NC_000913/2036951‑2037251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑