Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,640,1170GT48.2% 152.0 / inf 394R153R (CGC→CGA) hisShistidyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (113/91);  new base T (105/85);  total (218/176)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-02

Only 100 of 407 total aligned reads displayed.

GTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAACATCGGCCCGATATACCACAGACGC  >  NC_000913/2639967‑2640267
                                                                                                                                                      |                                                                                                                                                      
  tAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCcgcgcgcg                                                                                                                                                      >  7:237035/1‑151 (MQ=255)
  tAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgcgc                                                                                                                                                       <  2:668238/150‑1 (MQ=255)
    gtgtACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGcc                                                                                                                                                    <  5:1450582/151‑1 (MQ=255)
       tACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGccacc                                                                                                                                                 >  2:916071/1‑151 (MQ=255)
        aCATGCGGCGTTTGCCGGCGTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTCCGTGCTCGGAAATACCCAGCGCGCGccaccc                                                                                                                                                >  6:1166276/1‑150 (MQ=255)
        aCATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTCCTCGGAAATACCCAGCGCGCGccacc                                                                                                                                                 >  1:885530/1‑150 (MQ=255)
         cATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGccacc                                                                                                                                                 >  3:152831/1‑149 (MQ=255)
          aTGCGGCGTTTGCAGTCTTCGGCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGc                                                                                                                                              <  5:402879/151‑1 (MQ=255)
            gcggcgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCgg                                                                                                                                            <  2:296060/151‑1 (MQ=255)
             cggcgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCggg                                                                                                                                           <  6:1514065/151‑1 (MQ=255)
              ggcgTTTGAAGTCTACGTCCACCTTTTCTTTATGCTGCACAAGGAATGCCACCAGCGCATCGCGGAAAATGGCGCGTGCTTACAGCGAACCGATAGAGTTCAGCTAAAGAGTTACCTGCTCGGAAATACCCAGCGCTCGCCAACAGCGGGc                                                                                                                                          >  5:1325381/1‑151 (MQ=255)
                     gCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCAGCAGCGCATCGCGGTAATTGGCGCGTGCTTCAAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGc                                                                                                                                   <  7:92430/151‑1 (MQ=255)
                     gCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAg                                                                                                                                    <  7:619363/150‑1 (MQ=255)
                       aGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCAt                                                                                                                                 >  8:560659/1‑151 (MQ=255)
                        gTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCAt                                                                                                                                 >  4:454323/1‑150 (MQ=255)
                         tCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGCCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACCTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGAGAGCATa                                                                                                                                >  1:699532/1‑150 (MQ=255)
                          cTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCAAACAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAAt                                                                                                                              >  4:84634/1‑151 (MQ=255)
                             cGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCa                                                                                                                            <  2:253569/150‑1 (MQ=255)
                             cGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCa                                                                                                                            <  3:277252/150‑1 (MQ=255)
                             cGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAg                                                                                                                           <  5:1224378/151‑1 (MQ=255)
                             cGTCCAGCTTTTCTTTATGCTGCTCAACGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAg                                                                                                                           <  3:933520/151‑1 (MQ=255)
                              gtgcAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGt                                                                                                                          <  1:1001955/148‑1 (MQ=255)
                              gTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGt                                                                                                                          >  2:1105747/1‑151 (MQ=255)
                               tCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCCGTAATTGGCGCGTGATTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCTAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGtt                                                                                                                         >  2:739795/1‑151 (MQ=255)
                                ccAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTc                                                                                                                        >  7:171040/1‑151 (MQ=255)
                                ccAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTc                                                                                                                        <  2:562967/151‑1 (MQ=255)
                                  aGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTc                                                                                                                        >  6:938070/1‑149 (MQ=255)
                                    cTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCg                                                                                                                    >  8:1358856/1‑151 (MQ=255)
                                    cTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCg                                                                                                                    >  3:67013/1‑151 (MQ=255)
                                       ttCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCACCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTcc                                                                                                                 >  7:112211/1‑150 (MQ=255)
                                         cTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGat                                                                                                               >  8:1279394/1‑151 (MQ=255)
                                         atttATGCTGCTCAACGAATGCCACCAGATCATCGCGGTAATTGGCGAGTGCTTCCAGCGAACCGATAGAGTTCAGCTGAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGAAGTGAGCATAATCAGTTCATCGTCGat                                                                                                               <  1:357666/150‑1 (MQ=255)
                                           ttATGCTGCTCAAGGACTGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGa                                                                                                                <  7:354552/148‑1 (MQ=255)
                                           ttATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGatat                                                                                                             >  1:210205/1‑151 (MQ=255)
                                           ttATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGata                                                                                                              <  2:1395838/150‑1 (MQ=255)
                                            tATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATc                                                                                                            >  4:287255/1‑151 (MQ=255)
                                            tATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGatat                                                                                                             <  5:1084109/150‑1 (MQ=255)
                                            tATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATc                                                                                                            >  8:1536600/1‑151 (MQ=255)
                                               gctgctCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCgg                                                                                                         >  8:196728/1‑151 (MQ=255)
                                                ctgctCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCgg                                                                                                         >  7:1033049/1‑150 (MQ=255)
                                                  gctCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGa                                                                                                        <  1:756817/149‑1 (MQ=255)
                                                  gctCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGAcc                                                                                                      >  4:379048/1‑151 (MQ=255)
                                                    tCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCtt                                                                                                    >  5:6759/1‑151 (MQ=255)
                                                    tCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGAGAGCATAATCAGTTCCGCGTCCATATCCGGACCtt                                                                                                    >  4:545287/1‑151 (MQ=255)
                                                     cAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCtt                                                                                                    <  5:190622/150‑1 (MQ=255)
                                                     cAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTg                                                                                                   >  2:1594956/1‑151 (MQ=255)
                                                     cAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTg                                                                                                   <  2:968066/151‑1 (MQ=255)
                                                         gAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAgc                                                                                               >  5:288650/1‑150 (MQ=255)
                                                          aaTGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGAc                                                                                              <  1:1163785/151‑1 (MQ=255)
                                                                accaGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaaaa                                                                                        <  3:1358298/151‑1 (MQ=255)
                                                                  caGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACt                                                                                      >  1:422663/1‑151 (MQ=255)
                                                                    gcgcATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTc                                                                                    <  2:771639/151‑1 (MQ=255)
                                                                     cgcATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCg                                                                                   >  5:1399305/1‑151 (MQ=255)
                                                                      gcATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGc                                                                                  >  5:5472/1‑151 (MQ=255)
                                                                       cATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGc                                                                                  >  1:1471435/1‑150 (MQ=255)
                                                                           gcgGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCCGccc                                                                             >  4:1424654/1‑151 (MQ=255)
                                                                           gcgGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGccc                                                                             <  8:1145577/151‑1 (MQ=255)
                                                                             ggTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGCCCGAAAACTTCGCAGCCCa                                                                            >  7:702390/1‑150 (MQ=255)
                                                                              gTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAAc                                                                          >  7:1550766/1‑151 (MQ=255)
                                                                                  ttGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGAt                                                                      >  5:1572426/1‑151 (MQ=255)
                                                                                    ggCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATg                                                                     <  8:278834/150‑1 (MQ=255)
                                                                                       gcgTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAAc                                                                 >  1:450296/1‑151 (MQ=255)
                                                                                       gcgTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAAc                                                                 <  4:1057891/151‑1 (MQ=255)
                                                                                         gTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTg                                                               >  8:767944/1‑151 (MQ=255)
                                                                                              tCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGa                                                           <  6:1131055/150‑1 (MQ=255)
                                                                                              tCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACg                                                            <  3:614317/149‑1 (MQ=255)
                                                                                               ccAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGAt                                                          <  6:1307679/150‑1 (MQ=255)
                                                                                               ataGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATa                                                         <  8:642038/149‑1 (MQ=255)
                                                                                                 aGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAAc                                                       >  3:1161889/1‑151 (MQ=255)
                                                                                                  gCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACg                                                      >  4:743511/1‑151 (MQ=255)
                                                                                                   cGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGc                                                     >  7:1343846/1‑151 (MQ=255)
                                                                                                    gAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGcc                                                    >  1:591517/1‑151 (MQ=255)
                                                                                                      aCCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCt                                                  >  2:1312987/1‑151 (MQ=255)
                                                                                                      aCCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCt                                                  >  7:1250019/1‑151 (MQ=255)
                                                                                                      aCCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCt                                                  >  8:363395/1‑151 (MQ=255)
                                                                                                          aTAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCt                                              <  8:1214271/151‑1 (MQ=255)
                                                                                                          aTAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCt                                              <  8:1492851/151‑1 (MQ=255)
                                                                                                           tAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCt                                              <  6:1063449/150‑1 (MQ=255)
                                                                                                            agagTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGc                                            >  8:85952/1‑151 (MQ=255)
                                                                                                                 tCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCATACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGAc                                        <  3:1190656/150‑1 (MQ=255)
                                                                                                                  cAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGc                                      <  2:442881/151‑1 (MQ=255)
                                                                                                                  cAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGc                                      >  4:1598267/1‑151 (MQ=255)
                                                                                                                   aGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCt                                     <  1:1361188/151‑1 (MQ=255)
                                                                                                                       cAAGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCg                                   <  6:687217/149‑1 (MQ=255)
                                                                                                                       cAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTg                                 >  4:1228622/1‑151 (MQ=255)
                                                                                                                        aaGAGTTACGTGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTg                                 >  4:233508/1‑150 (MQ=255)
                                                                                                                           agTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACgg                             <  1:641706/151‑1 (MQ=255)
                                                                                                                              tACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCACCTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAAc                          >  1:954283/1‑151 (MQ=255)
                                                                                                                                  tGCTCGGAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAACATCg                      >  6:63243/1‑151 (MQ=255)
                                                                                                                                  tGCTCGGAAATACCAAGCGCGCGCCACAAGCCGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCAAACTGATGGAACTCACCATAACGCAATTTCTTCGGACTCACTAGACGGAACATCg                      >  5:1029296/1‑151 (MQ=255)
                                                                                                                                      cGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAACATCGGccc                  >  4:1144224/1‑151 (MQ=255)
                                                                                                                                      cGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCCGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAACATCGGcc                   <  7:449853/150‑1 (MQ=255)
                                                                                                                                       ggAAATACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAACATCGGCCCg                 <  8:1061904/151‑1 (MQ=255)
                                                                                                                                       ggAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAACATCGGCCCg                 <  5:760280/151‑1 (MQ=255)
                                                                                                                                       ggAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAACATCGGCCCg                 >  6:1252631/1‑151 (MQ=255)
                                                                                                                                         aaaTACCCAGCGCTCGCCACCAGCGGGCATTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAACATCGGCCCGa                <  6:523867/150‑1 (MQ=255)
                                                                                                                                           aTACCCATCGCGCTCCACCAGCTGGCAGTGAGAATAATCAGTTCAGCGTCGATATCCGTACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAACATCGGCCCGatat             <  5:895918/151‑1 (MQ=255)
                                                                                                                                           aTACCCAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGCCGCTCGTGACGGAACATCGGCCCGatat             >  6:302691/1‑151 (MQ=255)
                                                                                                                                               ccAGCGCTCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAACATCGGCCCGATATACca         <  8:503570/151‑1 (MQ=255)
                                                                                                                                               ccAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTTGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAACATCGGCCCGATATACca         <  7:1437935/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGCCCAACTGATGGAACTGACGATAACGCCCTTTCTGCGGACGCTCGTGACGGAACATCGGCCCGATATACCACAGACGC  >  NC_000913/2639967‑2640267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑