Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4320TG24.3% 868.6 / 71.6 413intergenic (+73/‑86)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (94/214);  new base G (98/1);  total (195/218)
Fisher's exact test for biased strand distribution p-value = 1.40e-38
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 438 total aligned reads displayed.

TTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTC  >  NC_000913/422282‑422580
                                                                                                                                                      |                                                                                                                                                    
 tCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcct                                                                                                                                                      <  7:1113208/150‑1 (MQ=255)
      tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTGCTccc                                                                                                                                                >  3:960498/1‑151 (MQ=255)
        gATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTccccc                                                                                                                                              >  6:385863/1‑151 (MQ=255)
              tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAata                                                                                                                                         <  3:1619102/150‑1 (MQ=255)
              tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCcaaaa                                                                                                                                         >  5:261018/1‑148 (MQ=255)
               gtggtgTTGATTGGCTGGATGTTTAAACGCCGCGAGGATCGTCAGCTGGCTGAAAACCAGTATTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATGCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTGCTCCCCCAAatat                                                                                                                                        <  6:1185883/150‑1 (MQ=255)
               gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAatat                                                                                                                                        <  4:64105/150‑1 (MQ=255)
                   tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCGGCCGCCCCCacagctccggc                                                                                                                                   >  4:1272241/1‑141 (MQ=255)
                    gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCa                                                                                                                                  <  1:1209075/151‑1 (MQ=255)
                       gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                               <  1:952833/151‑1 (MQ=255)
                        aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCGGTCCTCCGCCCCCCAATAGCCGTCCgagg                                                                                                                              >  2:407427/1‑149 (MQ=255)
                         ttGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCTTCAgatg                                                                                                                              >  4:504953/1‑150 (MQ=255)
                         ttGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCACAAATCCTGCAgatgc                                                                                                                             >  7:1336249/1‑150 (MQ=255)
                          tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatga                                                                                                                             <  2:603992/150‑1 (MQ=255)
                          tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatga                                                                                                                             <  8:187022/150‑1 (MQ=255)
                            gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                          <  3:314756/151‑1 (MQ=255)
                              tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCTGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGTCCTGCTCCCCCAAATATCCTTCAGATGATGAGt                                                                                                                        >  6:83643/1‑151 (MQ=255)
                                 aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCACATATCCTTCAGATGATGACTGa                                                                                                                      >  2:220134/1‑150 (MQ=255)
                                   gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGTAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATcc                                                                                                                   <  5:861783/151‑1 (MQ=255)
                                      tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                                 <  8:116449/150‑1 (MQ=255)
                                        aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCGTCCCCCAAATATCCTTCCGCTGATGAGTGGTCCTGcct                                                                                                              >  2:481389/1‑149 (MQ=255)
                                            cctccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                          <  3:1271247/148‑1 (MQ=255)
                                            ccgccACGATCGTCAGCTGGCTGAACACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGCTCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAAGCTCCTGCAGCTGATGAgggcgccgggagtagg                                                                                                          >  2:1768872/1‑136 (MQ=255)
                                            ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                          <  4:282138/151‑1 (MQ=255)
                                             cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc                                                                                                         <  6:1766312/151‑1 (MQ=255)
                                                cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCGGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCTCCCCGGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAt                                                                                                      <  4:1087143/151‑1 (MQ=255)
                                                 aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGGGCGCCGCCCCCCAATAACCAGCACATGAGGAGTGATCCGGGATTAGGCTagg                                                                                                     >  3:825472/1‑149 (MQ=255)
                                                  cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATgg                                                                                                    <  8:1007566/151‑1 (MQ=255)
                                                  cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATgg                                                                                                    <  8:74422/151‑1 (MQ=255)
                                                   gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                                   <  1:626338/151‑1 (MQ=255)
                                                   gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCACCTAGCCTGCAGATGAGGAGTGGTCCTGCATTTGGGGATggg                                                                                                   >  8:825684/1‑150 (MQ=255)
                                                    aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                                  <  6:654654/151‑1 (MQ=255)
                                                    aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTGCCCCCAATATCCTGATGATGATGAGGGATCGTGCATTAGGGTATGGc                                                                                                   >  3:1784064/1‑150 (MQ=255)
                                                    aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCCAATATCCTTCAGATGATGAGTGCTGCGGCATTAGGGTATGGcc                                                                                                  >  5:1460762/1‑150 (MQ=255)
                                                      cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAg                                                                                                <  6:1073652/151‑1 (MQ=255)
                                                      cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCCAATCTCCATCTGATGATGAGTGATCCTGCATTAGGCGATGGcg                                                                                                  >  5:65588/1‑148 (MQ=255)
                                                       gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                               <  1:796084/151‑1 (MQ=255)
                                                         cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCCAATATCCTTCAGATGAGGAGTGATGCTGCATTAGGGTATGGGAAGGTg                                                                                             >  7:1016222/1‑151 (MQ=255)
                                                         cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCGCCCACCGATCCGGCAGATGAGGCGTGATCCTGCATAAGGGTATGGCAACGTg                                                                                             >  4:404818/1‑151 (MQ=255)
                                                           gctgtctgAAAACCAGTAATCCTTGGGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt                                                                                           <  5:1608507/151‑1 (MQ=255)
                                                           gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCTCCCCCAAATATCCTTCAGAGGATGAGTGCTCCGGCATTTGGCGATGGCCAGGTg                                                                                             >  8:418970/1‑149 (MQ=255)
                                                             tggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCGGGGGCGCGGCCCCCCCCTATCCCGCCGATGCTGAGTGATCCTGCATTAGGCGATGGCATGGTGATcc                                                                                         >  8:291668/1‑150 (MQ=255)
                                                             tggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATc                                                                                          >  4:186638/1‑150 (MQ=255)
                                                              ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                          >  2:843071/1‑148 (MQ=255)
                                                               gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCGCCCAAATATGCTTCAGATGCTGAGTGATCCTGCATTAGGCTGTGGCAAGGTGCTc                                                                                          >  5:994534/1‑148 (MQ=255)
                                                                   aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtttt                                                                                   <  1:530464/151‑1 (MQ=255)
                                                                     aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                                 <  6:1467401/151‑1 (MQ=255)
                                                                       ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatc                                                                               >  2:570754/1‑151 (MQ=255)
                                                                        cattAATGCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCAGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                                              <  1:187194/148‑1 (MQ=255)
                                                                        cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                                              <  1:28691/151‑1 (MQ=255)
                                                                        cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                                              <  6:743896/151‑1 (MQ=255)
                                                                          gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca                                                                            <  6:1367523/151‑1 (MQ=255)
                                                                           tAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca                                                                            <  1:1752363/150‑1 (MQ=255)
                                                                            aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGGCGCCGCCCCCACAGATGCTTGAGATGATGAGTGAGCCTGCATGTGGGGATGGCAAGGTGCTCCGAGTTTGATCACAgg                                                                          >  2:1117475/1‑151 (MQ=255)
                                                                              tCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAAAAACCTTCAGATGATGTGTGCTCCTGGATTAGGGTATGGGAAGGTGATCAGATTTTCATCACAgggg                                                                        >  4:826816/1‑151 (MQ=255)
                                                                                  tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTCCGCCTGCCCCAAAGATCCTTGAGATGATGAGTGGTGCTGGCTTAGGCTAGGGCAAGGGGCTCAGATTTTGGTGAGAGGGGAGtt                                                                    >  4:1744698/1‑151 (MQ=255)
                                                                                  tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATCCGATTTTGATCGCAGGGGGAtt                                                                    >  2:1530051/1‑151 (MQ=255)
                                                                                       tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATGCTTCAGGTGATGGGTGATGCTGGGTTAGGGTATGGCTAGGGGGTCAGATTTTCATCACAGGGGATTTttgtt                                                               >  3:1120443/1‑149 (MQ=255)
                                                                                         tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATACTTGAGATGATGAGTGATCGTGCATTAGGCTATGGGAAGGTGAGCAGATTTTGGTCAGAGGGGAATTATGATGt                                                             >  6:1660151/1‑151 (MQ=255)
                                                                                          gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgatg                                                              <  5:463825/149‑1 (MQ=255)
                                                                                            aaGCCGGTTCAGGATGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTa                                                           <  7:636878/150‑1 (MQ=255)
                                                                                             aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                         <  8:1710445/151‑1 (MQ=255)
                                                                                             aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAAAAACCATAAGATGATGAGTGTTGCTGCAGTAGGATATGGCAAGGGGATGAGCTTTTGATCACTGTGGAATTATgttgtttat                                                         >  7:1179570/1‑148 (MQ=255)
                                                                                              gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGGCGGCCCCACATAACCTTCAGATGTTGTGGGCTCGTGCCTTAGGATATGGGGAGTGGTTCAGCGTTTGAGCACAGGGGAATTATGATGTTAAAt                                                        >  6:16120/1‑151 (MQ=255)
                                                                                                cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAGAACCGTCAGATGATGAGTGAACCAGCAATAAGAAAAGGCAAGGACAACAGAAATAAATCAGAGGGGAAGTATAAAGTTAAATg                                                       >  1:1423977/1‑150 (MQ=255)
                                                                                                 ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCGCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCGAGGGCAAGGTGATCAGATTTTCATCAGAGGGGAATTATGATGTTAAATGc                                                      >  1:385157/1‑150 (MQ=255)
                                                                                                  gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCGTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAGTAGGCTATGGGAAGGTGTTCAGCTTTTCTTCACAGGGGAGTTATGATGTTAAATGCAt                                                    >  4:745864/1‑151 (MQ=255)
                                                                                                   ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCTCCTACCCCAAATAACAATAAGATTATGAGTGATCAAGCATTAGGCTATGTGAACGTGATAAGATTTTAATAAAAAGGGAATTATTATGTTAAAttaatg                                                   >  1:1000563/1‑146 (MQ=255)
                                                                                                    tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTCATCCTGCAGTAGGCGATGGCAAGGTGATCAGGTTTTCATCAAAGGGGAATTATGATGTTAAATGCATgg                                                  >  1:1302678/1‑151 (MQ=255)
                                                                                                      aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTGCTCCCCCAAATATACTTCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATTGATGGc                                                 >  7:438537/1‑150 (MQ=255)
                                                                                                         acgaAAGCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCGAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                             <  1:1596510/149‑1 (MQ=255)
                                                                                                          ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTg                                            <  8:582834/151‑1 (MQ=255)
                                                                                                            aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATACTTCAGATGATGAGTGATCCGGCATTAGGCTATGGCAAGGTGATGAGATTTTCATCACAGGGGAATTATGATGTTAAATTAATGGCCCCTGcc                                          >  7:490535/1‑151 (MQ=255)
                                                                                                                   gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGc                                     <  3:682671/149‑1 (MQ=255)
                                                                                                                   gATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATAACCTTCAGATGATGGGTGATCCTGCATTAGGGTATGGCAAGGTGGTGAGATTTTCTTCACAGGGGAATTATTATGTTAAATGAATGGCACCTGCCgggggcc                                   >  7:178760/1‑146 (MQ=255)
                                                                                                                    aTCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTGCAGATGATGAGTGATGCTGCATTAGGCTATGGGAAGGTGGGCAGATTTTCATCACAGGGGAATTATGATGTTTAATGCATGGGACCTGCCGGTGccgc                                  >  8:1357811/1‑149 (MQ=255)
                                                                                                                      ccGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCGCCCCCAAATATCCTTCAGAGGATGAGTGATCGGGCATTAGGCTGTGGGAAGGGGATCAGAGTTTGATCACAGGGGCAGTATGATGTTAAATGCAGGGCACCGGCCGGGGcgcccc                                >  5:1308154/1‑150 (MQ=255)
                                                                                                                      ccGGCTATTCCGAAAGTTATCCGCCCCCCGCCGCCCCCCCCACCAATACATCAGAGGAAGAGTGAGCCGGCAAGAGGCGAGGGCAACGGGGGCCCATTGTAAGAACAGGGGCATGAAGATGTTAAATGACTGACACCGGCCGCTGcccccc                                >  5:1174768/1‑150 (MQ=255)
                                                                                                                       cGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAt                               <  3:397677/151‑1 (MQ=255)
                                                                                                                       cGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCATCAGAAGATGAGGGATACTGGATTAGGAGATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTATATGCATGGCACCGGCCGGGGCCCCCAt                               >  1:725120/1‑151 (MQ=255)
                                                                                                                        ggCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATAATTCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAAACACAGGGGAATTATGATGTTAAATGCAAGGCACCTCCCGGTGgcccc                                 >  4:1429568/1‑148 (MQ=255)
                                                                                                                             ttCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATAACCTTCAGATGATGAGTGATACTGCATAAGTCTATGGCAAGGTGATAATATTTTAATCAAAGGGGAATTATGAAGTTAAATTAATGTAACCTGCAGGTGAACCCAttaatt                          >  6:295923/1‑146 (MQ=255)
                                                                                                                               ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATACTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                        <  3:1550076/150‑1 (MQ=255)
                                                                                                                                cGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                      <  5:1716896/151‑1 (MQ=255)
                                                                                                                                   aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaa                   <  7:1684664/151‑1 (MQ=255)
                                                                                                                                    aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag                  <  7:362480/151‑1 (MQ=255)
                                                                                                                                    aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag                  <  7:859958/151‑1 (MQ=255)
                                                                                                                                        aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGCTGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGCGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa               <  4:1169636/150‑1 (MQ=255)
                                                                                                                                          ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATTATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag             <  8:470691/150‑1 (MQ=255)
                                                                                                                                           cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt           <  6:45854/151‑1 (MQ=255)
                                                                                                                                            gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt           >  8:1462211/1‑150 (MQ=255)
                                                                                                                                            gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAAAAAGCAAAGAt           >  3:518089/1‑150 (MQ=255)
                                                                                                                                             cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTCATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa         <  4:153866/151‑1 (MQ=255)
                                                                                                                                              ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa         >  2:29366/1‑150 (MQ=255)
                                                                                                                                              ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa         >  5:791499/1‑150 (MQ=255)
                                                                                                                                               cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc       >  8:1548634/1‑151 (MQ=255)
                                                                                                                                                ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt      <  5:53403/151‑1 (MQ=255)
                                                                                                                                                 cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg     >  2:1323581/1‑151 (MQ=255)
                                                                                                                                                    cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc  <  7:1503850/151‑1 (MQ=255)
                                                                                                                                                    cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc  >  4:579809/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTC  >  NC_000913/422282‑422580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: