Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4350TG23.4% 901.3 / 68.4 413intergenic (+76/‑83)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (97/217);  new base G (95/1);  total (195/218)
Fisher's exact test for biased strand distribution p-value = 2.08e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 435 total aligned reads displayed.

GCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTAC  >  NC_000913/422285‑422585
                                                                                                                                                      |                                                                                                                                                       
 cgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcctc                                                                                                                                                        <  1:710848/151‑1 (MQ=255)
    ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTcccc                                                                                                                                                     <  4:371596/151‑1 (MQ=255)
    ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGTCCTcccc                                                                                                                                                     >  6:340624/1‑151 (MQ=255)
     gATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGccccc                                                                                                                                                    >  7:406307/1‑151 (MQ=255)
         gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCGCCCCCAAat                                                                                                                                                >  4:612017/1‑151 (MQ=255)
              gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATcc                                                                                                                                            <  7:1563112/150‑1 (MQ=255)
                 gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCtt                                                                                                                                          <  4:1534983/149‑1 (MQ=255)
                 gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTc                                                                                                                                         <  3:1743648/150‑1 (MQ=255)
                 gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCa                                                                                                                                        <  1:1209075/151‑1 (MQ=255)
                 gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAAAATCCTTc                                                                                                                                         >  2:1076423/1‑150 (MQ=255)
                   tGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGTAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAg                                                                                                                                       <  6:446235/150‑1 (MQ=255)
                    gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                                     <  2:1633904/151‑1 (MQ=255)
                    gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                                     <  1:952833/151‑1 (MQ=255)
                     aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCGGTCCTCCGCCCCCCAATAGCCGTCCgagg                                                                                                                                    >  2:407427/1‑149 (MQ=255)
                       tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatga                                                                                                                                   <  2:603992/150‑1 (MQ=255)
                       tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatga                                                                                                                                   <  8:187022/150‑1 (MQ=255)
                           tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCGTCAGATGATGAGt                                                                                                                              >  5:129931/1‑151 (MQ=255)
                              aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCACATATCCTTCAGATGATGACTGa                                                                                                                            >  2:220134/1‑150 (MQ=255)
                               tGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                          >  4:184933/1‑151 (MQ=255)
                                    aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGc                                                                                                                      <  1:54075/150‑1 (MQ=255)
                                     aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCGTCAGATGATGAGTGGGCCTGCAt                                                                                                                    >  7:777299/1‑151 (MQ=255)
                                     aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCGTCCCCCAAATATCCTTCCGCTGATGAGTGGTCCTGcct                                                                                                                    >  2:481389/1‑149 (MQ=255)
                                      aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAAAATCCTTCAGATGATGAGTGATGCTGGAtt                                                                                                                   >  4:258095/1‑151 (MQ=255)
                                       cgccgcCACGATGGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTa                                                                                                                  <  6:700382/151‑1 (MQ=255)
                                          cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc                                                                                                               <  3:899540/151‑1 (MQ=255)
                                          cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCCAATATACTTCAGATGATGAGTGATCCTGCATTAggg                                                                                                               >  4:1386573/1‑150 (MQ=255)
                                               cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATgg                                                                                                          <  8:74422/151‑1 (MQ=255)
                                                gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                                         <  1:626338/151‑1 (MQ=255)
                                                 aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCCAATATCCTTCAGATGATGAGTGCTGCGGCATTAGGGTATGGcc                                                                                                        >  5:1460762/1‑150 (MQ=255)
                                                   cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAg                                                                                                      <  6:1073652/151‑1 (MQ=255)
                                                   cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCCAATCTCCATCTGATGATGAGTGATCCTGCATTAGGCGATGGcg                                                                                                        >  5:65588/1‑148 (MQ=255)
                                                   cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATAAACTTCAGATGATGAGTGATGCTGCATTAGGCTATTGCa                                                                                                        >  2:560321/1‑149 (MQ=255)
                                                    gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                                     <  1:796084/151‑1 (MQ=255)
                                                    gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                                     <  6:1617535/151‑1 (MQ=255)
                                                       aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAAAATCCTTCATATGATGCGTGATGGTGCATTAGGCTATGGCTAGGTgt                                                                                                  >  7:983750/1‑150 (MQ=255)
                                                        gctgtctgAAAACCAGTAATCCTTGGGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt                                                                                                 <  5:1608507/151‑1 (MQ=255)
                                                        gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt                                                                                                 <  7:575660/151‑1 (MQ=255)
                                                        gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCTCCCCCAAATATCCTTCAGAGGATGAGTGCTCCGGCATTTGGCGATGGCCAGGTg                                                                                                   >  8:418970/1‑149 (MQ=255)
                                                        gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCACATATCCTTCAGATGATGCGTGATGCTGGGTTAGGGTATGGCAAGGTggt                                                                                                 >  4:1140505/1‑149 (MQ=255)
                                                         cgggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTGGAGAGGATGAGTGATGCTGCATTAGGCGATGGCAAGGTGATc                                                                                                >  2:151132/3‑151 (MQ=255)
                                                          tggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCGGGGGCGCGGCCCCCCCCTATCCCGCCGATGCTGAGTGATCCTGCATTAGGCGATGGCATGGTGATcc                                                                                               >  8:291668/1‑150 (MQ=255)
                                                            gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGGCCGCCGCCCCCCCATATCCTTCAGATGATGAGGGATCCGGGCTTAGGCTTTGGCCAGGTGCTCAgc                                                                                             >  7:1779753/1‑150 (MQ=255)
                                                            gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCGCCCAAATATGCTTCAGATGCTGAGTGATCCTGCATTAGGCTGTGGCAAGGTGCTc                                                                                                >  5:994534/1‑148 (MQ=255)
                                                                aaaaCCAGTATTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTGCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATAAGAtttt                                                                                         >  4:1060662/1‑151 (MQ=255)
                                                                     cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                                                    <  1:28691/151‑1 (MQ=255)
                                                                     cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCGTCCCCCAAATATCCTTCAGATGATGAGTGCTCCTGCATTAGGGTGTGGGAAGGTGGTCAGATTTtcatc                                                                                     >  3:1807755/1‑150 (MQ=255)
                                                                       gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca                                                                                  <  6:1367523/151‑1 (MQ=255)
                                                                        tAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca                                                                                  <  1:1752363/150‑1 (MQ=255)
                                                                          aTCCTTGCGTGCTGTAAGCCGGTGCAGGAGGAAATCCTCATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAggg                                                                               <  6:753934/151‑1 (MQ=255)
                                                                           tCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAAAAACCTTCAGATGATGTGTGCTCCTGGATTAGGGTATGGGAAGGTGATCAGATTTTCATCACAgggg                                                                              >  4:826816/1‑151 (MQ=255)
                                                                               tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCAAATATGCTTCAGATGATGAGTGGTGCTGCATTATGCTTTGGCAAGGTGATCAGATTTTGATCACAggggtttt                                                                          >  3:751887/1‑147 (MQ=255)
                                                                               tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                           <  8:1713189/150‑1 (MQ=255)
                                                                                 cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTGCGCCCCCCAATATCCTTCAGATGATGGGTGGTCCTGCCGTAGGGTATGGGACGGTGCGCAGAGTTTGATCACAGGGGatgttt                                                                        >  8:1398024/1‑146 (MQ=255)
                                                                                  gTTCTGTAAGCCGGTTCAGGAGTAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                                                      <  8:1011571/151‑1 (MQ=255)
                                                                                    tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGGGTGATCCTGCATTAGGGTATGGCATGGTGAGCAGAGTTTCATCGCTGGGGAATTatgat                                                                     >  2:268520/1‑151 (MQ=255)
                                                                                      tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCGTCAGATGATGAGTGATCCTGCATTTGGGTATGGGAAGGGGTGCAGATTTTATTCACAGGGGGATGATGATGt                                                                   >  6:1532526/1‑151 (MQ=255)
                                                                                       gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgatg                                                                    <  5:463825/149‑1 (MQ=255)
                                                                                       gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                                   <  7:1373438/150‑1 (MQ=255)
                                                                                       gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                                   <  3:1241680/150‑1 (MQ=255)
                                                                                       gTAAGCCGGGTCAGGAGGAAATCCGGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                                     <  2:740538/148‑1 (MQ=255)
                                                                                         aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTa                                                                 <  8:822276/150‑1 (MQ=255)
                                                                                            ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                             <  4:1612485/151‑1 (MQ=255)
                                                                                             cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAGAACCGTCAGATGATGAGTGAACCAGCAATAAGAAAAGGCAAGGACAACAGAAATAAATCAGAGGGGAAGTATAAAGTTAAATg                                                             >  1:1423977/1‑150 (MQ=255)
                                                                                              ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCGCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCGAGGGCAAGGTGATCAGATTTTCATCAGAGGGGAATTATGATGTTAAATGc                                                            >  1:385157/1‑150 (MQ=255)
                                                                                               gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                          <  7:1066805/151‑1 (MQ=255)
                                                                                               gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                          <  3:1496969/151‑1 (MQ=255)
                                                                                                ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCTCCTACCCCAAATAACAATAAGATTATGAGTGATCAAGCATTAGGCTATGTGAACGTGATAAGATTTTAATAAAAAGGGAATTATTATGTTAAAttaatg                                                         >  1:1000563/1‑146 (MQ=255)
                                                                                                 tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTCATCCTGCAGTAGGCGATGGCAAGGTGATCAGGTTTTCATCAAAGGGGAATTATGATGTTAAATGCATgg                                                        >  1:1302678/1‑151 (MQ=255)
                                                                                                   aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCCCCGCCGCGGCCCCCCAATAACCTTCAGAAGAACAGAGTTAGTGCATTATGGTATTGCAAGTTCATCAGAGATTGATCACAGGGCAATTATGATTATAAATCCaatga                                                       >  7:288174/1‑146 (MQ=255)
                                                                                                   aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTGCGCCTCCCCCCAATATCCTTCAGATGATGAGTGTGGCTGCATGAGGGGATGGCCTGGTGCTCCGATTTTCATCACAGGGGAATGTTGATGTTAATTGccaggcc                                                      >  3:467333/1‑145 (MQ=255)
                                                                                                      aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAGTTATGATGTTAAATGCATGGCACCt                                                   >  6:184447/1‑151 (MQ=255)
                                                                                                      acgaAAGCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCGAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                                   <  1:1596510/149‑1 (MQ=255)
                                                                                                       ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGGCCGCCGCCCCCAACTAAGCTACCGATGATGAGTGCTCCTGCAGTAGGCTATGGCAGGGGGAGCCGGGTGTCATAACAGGGGAATTATGTTGTTAAATGCATGGCAccgg                                                  >  6:865164/1‑149 (MQ=255)
                                                                                                       ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTg                                                  <  8:582834/151‑1 (MQ=255)
                                                                                                            accTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGACTCCTCCCCCAAATATCCTTCATATGATGATTGATCCTGCATTATGCTATGGCAATGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑Gt                                            <  6:170529/150‑1 (MQ=255)
                                                                                                                gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGc                                          <  8:1215598/149‑1 (MQ=255)
                                                                                                                   ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCa                                     <  4:1219385/151‑1 (MQ=255)
                                                                                                                   ccGGCTATTCCGAAAGTTATCCGCCCCCCGCCGCCGCCCCCCAAGATCCGTCAGATGATGAGTGATCCTGCATTAGGCTAGGGCAAGGGGATCAGAGTTTAATCACAGGGGAATTATGATGTTAAATGCAAGGCACCCGCCGCGGGccccc                                      >  5:1166389/1‑151 (MQ=255)
                                                                                                                     ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCAtt                                   <  3:1580505/151‑1 (MQ=255)
                                                                                                                     ggCTAATCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGACTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCAtt                                   <  1:1825403/151‑1 (MQ=255)
                                                                                                                         aTTCCGAAAGATATCCGCCCCCGACCTCCGGCCCCAAAAATGGGTCAGATGATGAGTGATTCTGCATTAGGCTATGGCAAGGTGGTCAGATTTTATTCACATGGGGATTAAGATGATTAATTCTTGGCACCTGCAG‑GTGGAGCCATATGtt                               >  4:457597/1‑151 (MQ=255)
                                                                                                                          ttCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAAAATCCTTCAGATGATGACTTATACTGCATTAGTCTATGGCAAGGTGATCAGATTTTGATAACAGGGGAATTATTATGTTAAATTCATTGCACCTCCCG‑GTGCCCCCAtttttt                               >  7:47610/1‑150 (MQ=255)
                                                                                                                             cGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAAc                           <  5:1716896/151‑1 (MQ=255)
                                                                                                                             cGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCATTAAGATGATGAGTGATCCTGCATTAGGATATTGCAAGGTGTTCAGATTTTCATCAAAGGGGAAGTATGATTTTAAATGAATGGCACCTGCCG‑GTGCCCCCCTGTGTTaaa                            >  3:353248/1‑150 (MQ=255)
                                                                                                                                 aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAAcaaag                       <  3:599505/151‑1 (MQ=255)
                                                                                                                                  gTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAAcaaagc                      <  7:1175192/151‑1 (MQ=255)
                                                                                                                                       ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATTATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAAcaaagcaaag                  <  8:470691/150‑1 (MQ=255)
                                                                                                                                       ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGa                 <  4:559351/151‑1 (MQ=255)
                                                                                                                                         gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGAt                >  8:1462211/1‑150 (MQ=255)
                                                                                                                                           ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATCa              >  5:791499/1‑150 (MQ=255)
                                                                                                                                            cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc            >  8:1548634/1‑151 (MQ=255)
                                                                                                                                             ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt           <  5:353369/151‑1 (MQ=255)
                                                                                                                                             ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt           >  2:974831/1‑151 (MQ=255)
                                                                                                                                             ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt           <  5:53403/151‑1 (MQ=255)
                                                                                                                                              cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg          >  2:1323581/1‑151 (MQ=255)
                                                                                                                                              cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg          <  5:879155/151‑1 (MQ=255)
                                                                                                                                                tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCt        >  7:1257759/1‑151 (MQ=255)
                                                                                                                                                gcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCt        <  6:1217880/150‑1 (MQ=255)
                                                                                                                                                 cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCt        <  6:489384/150‑1 (MQ=255)
                                                                                                                                                  ctcctacCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATAAACTGATc       >  4:657197/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
GCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCG‑GTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTAC  >  NC_000913/422285‑422585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑