Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,7580GC28.0% 127.0 / 37.7 129E614Q (GAA→CAA) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base G (41/44);  new base C (7/26);  total (52/77)
Fisher's exact test for biased strand distribution p-value = 1.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

CCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAA  >  NC_000913/1708610‑1708906
                                                                                                                                                       |                                                                                                                                                     
ccGCGCCAAAGCGCGCAAGCTGGAACATCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAgaagaa                                                                                                                                                     <  7:387881/151‑1 (MQ=255)
 ggcgacacatgtcgcAATCTGGAAGAGCATCAGGATAATCCGGAACCAGCACCCCATGCGGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAAc                                                                                                                                                    <  1:156928/139‑1 (MQ=14)
 cgcgACAAACCGCGCAAGCTGGAACACCAACAGGCTAATCCCGAACCAGAACAACAGGTCGATCCCCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGGG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAAc                                                                                                                                                    <  1:1721359/151‑1 (MQ=14)
    gcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAgg                                                                                                                                                 <  8:1488318/151‑1 (MQ=40)
      aaaatctcgcAAGCTGGAACAGCAACAGGATAATGCGGAACCAGAACAAGAGGGCGATCCCCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTc                                                                                                                                               <  4:490444/144‑1 (MQ=14)
       aaatctcgcAAGCTGGAACATCATCAGGCTAATGCGGAAGCAGAACAACAGGTCGATCCTCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCg                                                                                                                                              <  6:1762688/145‑1 (MQ=14)
       aaaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCCGCAGTAGCCGCGGCTATTGCCCGCG‑CGAAGGCAC‑AAAAACACGACCAGCA‑GAAGGCTAATCCCGGAGCAGAAGAGCAgcgtg                                                                                                                                              >  6:1252875/1‑147 (MQ=2)
       aaaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTGGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCg                                                                                                                                              <  4:805818/151‑1 (MQ=255)
           cgcgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATcc                                                                                                                                          <  1:89755/151‑1 (MQ=40)
           cacgcAAGCTGGAACAGCAACAGGCTAATGCGGACCCAGAACAACAGCTCTATCAGCCCCAACCGGCAGTAGAACCCGCAAATGAACCAG‑CCAACTCGC‑CCGTCCTCGAACAGCA‑ACAGGATAATGCGGAGCCAGACGACCAgggggcgcc                                                                                                                                          >  3:680454/3‑144 (MQ=2)
              gtaatcaggAACATCTACAGGATAATTACGAAGGAGCACTAGATGTTGATCCTCGTAAACCCGCCGTCGAAGCTGCTATAGCCCGAG‑CCAAAGCTC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg                                                                                                                                       <  5:907306/144‑1 (MQ=2)
              gcAAGCTGGAAGATCAACAGGCTAATGCGGAACCAGAAAAACAGGTCGATCCCCGCAAAGTCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCcgcg                                                                                                                                       <  2:1770369/151‑1 (MQ=255)
              gcAAGCTGGAACATCAACAGGCTAATCCCGAACCAGAACAACAGGGCGATCCCCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGCG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCcgcg                                                                                                                                       <  3:1733046/151‑1 (MQ=14)
               gaaGCTGGAACATCTACAGGATAATGCGGACCCAGAACCAGAGGGGGATCCCCGCAAACCCGCCGTCGAAGCAGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCgcgc                                                                                                                                      <  3:97278/150‑1 (MQ=14)
                aaGCTGGAACAGCAACCGGCTAATGCGGAACCAGAACAACAGGTCTATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAG‑CCAAAGCGC‑GCAAGCTGGCACAGCA‑ACGGGCGCATGCGGACCCCGCTGAACAGGTCGCTCCgcgcc                                                                                                                                     >  2:1301324/1‑150 (MQ=14)
                  ccTGGAACACCAACAGGCTACTGCGGACCCAGAACAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATGGCCCGCG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCaaa                                                                                                                                   <  2:1347169/150‑1 (MQ=14)
                     ggAACATCTACAGGATAATTCGGAAGCAGAACAAGTGGTCGATCCGCGCAAAGCGGCCGTCGAAGCAGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgcc                                                                                                                                <  4:1656514/151‑1 (MQ=14)
                         catcatcAGGATAATGCGGAACCAGAAAACGAGGCCGATCCCCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGccgccg                                                                                                                            <  4:1459309/145‑1 (MQ=14)
                            cAACAGGCTAATGCGGAACCAGAACAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCg                                                                                                                         <  8:1188338/151‑1 (MQ=255)
                             aaCAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAATGCCGCCGTCGAAGCCGCTATTGCCCGAG‑CCAAAGCCC‑GCAAGCTGAGAGAGCA‑AGAGCAGACGGCTGAACGACAGTCACAGGTAGCTCCGCCCAAAGCCGGCGTCgc                                                                                                                        >  2:934880/1‑150 (MQ=2)
                              acagcatAATCCGGAACCAGAAAACCAGGCCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGaa                                                                                                                       <  7:609020/145‑1 (MQ=14)
                              aCAGGATAATCAGGAAGCAGAACCAGTGGTCGATCCTCGTAAATCTGCCGTGGAAGCAGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGaa                                                                                                                       <  1:1479789/151‑1 (MQ=11)
                               caggatactcccgAAGCAGAACACCAGGCGGATCCGCGCAAAGCAGCCGTCGAAGCAGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                      <  2:766080/139‑1 (MQ=14)
                               cAGGCAAATTCGGAAGCAGAACACGTGGTCTATCCTCGCAAATCTGCCGTTGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                      <  1:705936/151‑1 (MQ=14)
                                 cccaacttccgAACCAGCAAAACATGCCGATCCTCGCAAAACGGCCGTCGAAGCAGCTATTGCCCGGG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGcc                                                                                                                    <  1:20998/141‑1 (MQ=11)
                                    tAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGACCCAGAAGACCAGGTCGATCCGCGCAAAGACGCCGTCGAAGCCGCt                                                                                                                 >  2:93636/1‑151 (MQ=255)
                                     aaTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTTGCAGCGCCTAATGCCAG‑A‑CCAAACCCCAGCAGGCAGGAACAGCT‑AGAGCCTAATCCGGAGGCAGAATGACACCTAGATCCGCGCCAAGcggccggggcggcggggc                                                                                                                >  7:7772/1‑139 (MQ=2)
                                      ataacgAACCAGAACCCCCGGATTATCCTCGCAAAACTGCCGTCGAAGCAGCTATTTCCCGGG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAt                                                                                                               <  4:1473542/146‑1 (MQ=11)
                                      aTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCG‑CAAAAGCCC‑GAAAACGGGAACAGCA‑ACAGGCTAATGCCGACCCAGAAGAACCGCTCGATACGCGAACAGCCGCCGTAGCCGCGGCTAt                                                                                                               >  3:1209013/1‑151 (MQ=11)
                                         cGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAGCAAGAAGAACACGTCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTg                                                                                                             >  4:1015857/1‑150 (MQ=255)
                                           gAACCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCGATTGCCCGGG‑CCAAAGCTC‑CCAATCTGGACCAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTGGATCCGCGCAAAGGCGCGGTCGAGGCCGcgtgtgccc                                                                                                          >  2:1100436/1‑143 (MQ=14)
                                            aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGCG‑CGAAAGCCA‑GCAAGCACGAACAGCA‑ACAGGATAATGAGCTCCGAGAAGACCAGGTTGATCCGAGCAAAGAGGAAGTGGACGAGGCTATTGCCCg                                                                                                         >  1:751261/1‑151 (MQ=2)
                                             cccAGAACACCAGGTGGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGGG‑CCAAAGCGC‑GCAAGCTGGAACACCA‑ACAGGCTAATGTGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGt                                                                                                        <  8:370759/150‑1 (MQ=11)
                                             aCCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGAG‑CCAAAGCAC‑GCAAGCTGGAA‑AGAACAGAAGCTAGTCCTGAACGGGAAGAAAATATGGATCCGAGGAAATCTGCCGTCGAAGCGGCTaattaaaa                                                                                                         >  1:1656798/1‑143 (MQ=2)
                                             aCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCTCTATTGCCCGTC‑CAAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCAGCCGTCGAAGCCGCGATAGCCCgg                                                                                                        >  1:210298/1‑150 (MQ=11)
                                              ccAGCACCACAGGGCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTg                                                                                                       <  2:1300314/151‑1 (MQ=255)
                                                agaagaACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAG‑CAAAAGCCC‑ACAAGCTGGACCAGCA‑AAAGGCTAATGAGGACCAAGAAGAACAAAAGGATCAGCGAAAAGCGGAAGTTGAAGCGGCTATTcccagcgc                                                                                                      >  6:1258430/1‑145 (MQ=2)
                                                  aacaacAGTTCGATCCTCTCAAAGCCTCCTTCTATGCCTTTATATCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCa                                                                                                    <  6:875989/150‑1 (MQ=14)
                                                   aaaacaGGCTGATCCTCGAAAACCCGCCGTCGAAGAAGCTATTTCCCGGG‑ACAAATCTG‑GCAAGCTGGAACAGCA‑ACAGGATAATCCGGAACCAGAACAACAGGCCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCaaa                                                                                                  <  1:150276/149‑1 (MQ=11)
                                                       catgtagATCCTCGGAAAACTGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCTC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAACAACAGGGCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CAc                                                                                             <  3:1408734/145‑1 (MQ=11)
                                                           gccccccGCGCACCCCCGCCGGCGACGCCGCTATTTCCCGAG‑CCAAAGCCC‑GCAAGCGCGATCCGCT‑AACGTCTAATGAGTACAGAGGAGTCCTCTTCGCATCTCGGAAACCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAG‑CGCGCaa                                                                                         <  2:634876/146‑1 (MQ=0)
                                                            cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAAGACCATCCTCGCACAGAAGCCGGAGAATCAGCTAGTGCACGAGCCAAAG‑CACGCaa                                                                                         >  6:586632/1‑150 (MQ=11)
                                                             gagccgctcacacctgccgTCTAAGCATCTATTTCCCGGGAACAACGC‑C‑GCAACCTGGAACACCA‑ACAGGATAATGAGGAACCAGCACACCAGGGCGATCCCCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGc                                                                                       <  3:867318/136‑1 (MQ=2)
                                                              aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTGGATCCGCGCAAAGCGGCAGTAGCCGCCGCTATTGCCCGCGCGAACG‑ccaacaaacc                                                                                      >  7:685031/1‑142 (MQ=11)
                                                              aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑CCAGGCTAATGCGGATCCAGACGACGAGGGAGATCCGCGCACAGCTGCCGTCGAAGCCGCTATTGCACGCGCCACAG‑CCCGcccgcg                                                                                      >  2:1618745/1‑146 (MQ=11)
                                                                  ccgcaaatcttcagTCGAACCCGCTAGGGCCCGCG‑CCAAAGCGC‑GAAAGCTGGACCAGCA‑ACACGCTACTGCTGAACAACAACAACAGGGCGATCCCCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGaa                                                                                  <  3:387431/138‑1 (MQ=2)
                                                                   cgcAAACCTGCCGGAGAAGCAGCTATTTCACGGG‑CCAAACGGG‑GCAAGCTGGAACACCA‑ACAGGATACTCCGGAAGCAGCACCCCAGGCCGATCCCCGCAAACCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAAc                                                                                 <  3:164739/151‑1 (MQ=12)
                                                                      aaatctgcaggagAAGCCGCTATAGCCCGTG‑CCATCGCGC‑GCAAGCTGGTAACCCC‑ACTGCCCTATGGTGAACCAGAACCCCAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAg                                                                               <  8:760802/138‑1 (MQ=2)
                                                                      aaaGCCGCCGTCGAAGCCGCTATTGCCCGAG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATAACGAAACAGAATAAAAGATGGAAACGCGCTAAGCTACCGTCGAAGACGCTATTGACCGAGCAAAAG‑AAAAAAAAATGGAACAGc                                                                              >  4:528051/1‑151 (MQ=0)
                                                                        acttgcagTCGATGCAGCTATTTACCGTG‑ACAAATGTC‑GCAATCTGGAACAGCA‑ACAGGCTAATTAGGAACCAGAACCAGATGGCGATCCTCGGAAATCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCaa                                                                            <  5:1515826/144‑1 (MQ=2)
                                                                         gccgccGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACACGCTAGTGCGCGCCCAGCACAGCAGGGCGCTCCGCGCAAGGGAGCCGGCGAAGCGGCTAGTGCGCGAGCCAAAG‑CCCGCAAGCTggccccgcccc                                                                           >  8:518980/1‑142 (MQ=2)
                                                                         cccgccgGCGAAGCCGCTATTCCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAAc                                                                           <  8:584828/150‑1 (MQ=255)
                                                                          ctgccgTTGAAGCAGCTATCGCCCGCG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGACAAAGCAAGAAATTCCAGTACCAAAA‑CACTCAAGCAGAAACAggacc                                                                           >  1:1259401/3‑146 (MQ=14)
                                                                           cgtagTCGAATCCGCTATTCGCCGCT‑CTTAAGAGC‑TGAACCAGGAAGAGCA‑TCAGGATAATTCGGACGCAGAACAAGTGGGTGATCCTCGTAAATCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAg                                                                         <  6:1440059/147‑1 (MQ=2)
                                                                           cgccgGCGACGCCGCTATTGCCTTTT‑TAAATGCTC‑GGAAGCGGGAACAGAT‑ATAGCCTAATGCGTACACTGTTTAACTCGTCGATGCTCTTAATTTCTCCGTCGAAGCGGCTATTGCACGCGCCAAAG‑CACGCAAGCTGGAACAGCAACAg                                                                         <  6:1278480/151‑1 (MQ=2)
                                                                            gccgTAGAAGCCGCTATTGCCCGTG‑CCAAATCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAgg                                                                        <  2:1016954/151‑1 (MQ=255)
                                                                              cgTCGAAGCAGCTATTCCCCGGG‑CCAAACCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCt                                                                      <  2:2175/151‑1 (MQ=255)
                                                                               gggcaaGCCGCTATTGCCCGTT‑CCAACGCGA‑GCAAGCTGGACCAGCT‑ACAGGCTTCTGAGGATCCAGCAGAACTGGTCTATTCGCGCATAACGGCCGTCGAAGCCGCTATAGCCCGGGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTa                                                                     <  2:517912/147‑1 (MQ=9)
                                                                               gTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑GCACGCTAAAGCGGCGGCAAAAGAAAAGGTGTATCATAGCAAGGAGGACTTCGAAGACGCTATTGCACTATAAAAAG‑CACGCAAGCTggcgccgcagggggggt                                                                     >  5:412626/1‑136 (MQ=2)
                                                                                 cgcaGCCGCTATTTCCCGTC‑ACAAAGCCC‑TCCTCATAGAACAGCC‑ACTGTCTTCTGCGGAGCGACTAGCTGAGGTCTTGCCGGGTAAAGCCGCCGTCGAAGCGGCTATTGCACGCGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTa                                                                     <  2:528955/146‑1 (MQ=2)
                                                                                   aaGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATACATAGCAAGAACAACATGTAGATAAACGCAAATCGAAAGTCTAAACCGCTATTGCCAGGGCCAAAG‑AAATAAAAAAAGAACAGCAGCAGGCTCATgg                                                                 >  1:1257191/1‑150 (MQ=2)
                                                                                     gaagCTATTGAACGGG‑CCTAATCTC‑GGAAGCTGGAAGAGCA‑TCAGGCTAATGCGGAAGCAGAACAACAGGTCGATCCTCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGcc                                                                <  4:1603317/147‑1 (MQ=11)
                                                                                        gCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGACCCAGAAGAACAGGTAGATCCGCGCAAAGCGGCCGTAGACGCGGCTATTGCCCGAGCCCACG‑CACCAAAACCCGACCAGCACCAGGATCATCCCGAGc                                                            >  2:1450955/1‑151 (MQ=11)
                                                                                             cgCCCGCG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGCAGCGGCTATTGCCCGAGCGAAAG‑CCAACAACCACGACCAGCACAAGGATAAAACCGAGCCAGa                                                        >  4:1713589/2‑150 (MQ=2)
                                                                                              gCCCGGG‑CCTAAGCCC‑GCAAGGGGTAAGATCA‑ACAGGATAATCACGAACCAGCACCACAGGGCGATCCTCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa                                                       <  2:732021/150‑1 (MQ=11)
                                                                                                  cgggaataaactc‑gcAATCTGGAACAACT‑ACAGGATAATTAGGAACCAGAACCCGTGGCTGATCCTCGTAAATCTGCCGTTGAAGCAGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaac                                                   <  5:1441275/139‑1 (MQ=2)
                                                                                                  cggcaccccaccc‑gcAATCAGGAACACCA‑ACAGCCTACTCCCGAACCAGCACCCCAGGCCGATCCCCGCAAACCCGCCGTCGAAGCCGCTATCGCCCGCGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaac                                                   <  3:165293/139‑1 (MQ=2)
                                                                                                   gtgaaaaatctc‑gcAAGCTGGAAGATCA‑TCAGGATAATACGGAAGCAGAATAACTTGCCGATCCTCGCAAAGCGGCCGTTGAAGCAGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca                                                  <  6:1705092/140‑1 (MQ=14)
                                                                                                      gaataatgtgggaatcTGGAAGATCT‑ACAGGATACTTAGGAAGCAGAATAAGAGGTTGATCCTCGTAAATCTGCCGTCGAAGCAGCTATTGCCCGTGCCAAAG‑CTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                                                <  1:1132991/136‑1 (MQ=2)
                                                                                                       cAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGACCCAGAAGAACAGGACCAACCAAGCAAATCCGCCATCGAAACCGATATTGCAAGTGACAACGACAC‑CAAGCTGGCCCAGAAACAATCAAAAACAGAGCCAGAAGAACAgggg                                              >  4:723649/1‑149 (MQ=2)
                                                                                                       cAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGAAAAGCCGCCGTCGAAGACGCTATTGCACGAGCCAAAG‑CACGCAAGCTCGAACAGCAACCGGCTAATGCAGAGCCAGAAGAACAGGtg                                              >  4:90932/1‑150 (MQ=11)
                                                                                                       cAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGATAATGCGGAACCAGAACAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc                                              <  2:402461/151‑1 (MQ=255)
                                                                                                          aGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCgcgc                                                                                                                                      >  2:750206/1‑61 (MQ=21)
                                                                                                             gc‑gcAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTGGATCCGCGCAAAGCCGCCGTGGCCGCCGCTATTGCCCGCGCGAACG‑CCCGAAACCGCGCCCAGCACCAGGGTCTAGACGAGCCAGaactccacgtctcacgc                                        >  8:814415/1‑137 (MQ=2)
                                                                                                             gc‑gcAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTGGCCGCGGCTATTGCCCGCGCGCACG‑CCAGAAAGCCGGCCCAGCACCAGGCTGAAGCCGAGCCCGaagacgcggtcgctccc                                        >  3:105495/1‑136 (MQ=2)
                                                                                                              gggcAAGCAGGAACACCT‑ACAGCAAAATCCCGAAGCAGAACCAGAGGGCGATCCTCGCAAACCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcg                                        <  7:1666448/149‑1 (MQ=11)
                                                                                                                 cAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCAAAAG‑CACGCAAACACGAACACCAACAGGCTAATGACGAGCCAGAACAACAGGTAGATCAgcgc                                     >  4:714257/1‑151 (MQ=14)
                                                                                                                   aGCTGGAAGAGCT‑TCAGGATAATTCGGAACCAGAACACGTTGGCGATCCGCGCAAATCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa                                   <  4:385610/151‑1 (MQ=11)
                                                                                                                   aGCTGGAACAGCA‑ACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCAAAAG‑CCAAAAAACCCGAACAGCAAAAGGATAAAAACGAGCAATAaaaacaattaaaaaaaacaa                                    >  1:1667730/1‑136 (MQ=2)
                                                                                                                    gCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCGAATG‑CACCCAAGCGGGAACAGCAACAGGCTAATGCCGAGCAAGAACTCCACGTCGCTCCGCGCac                                   >  3:387286/1‑149 (MQ=14)
                                                                                                                      tGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCGGCCGTGGCAGCGGCTATTGCCCGAGCCAACG‑CCCGCAAGCCGGACCAGCACAAGGCTGTTGACGAGCCCGAACTGCACGTCGATCAGCGGAAAgc                                >  8:1194595/1‑151 (MQ=9)
                                                                                                                       ggAAGAACA‑ACAGGAAAATGCCGAACCAGAACCCCAGGCCGATCCCCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgcc                               <  2:538194/151‑1 (MQ=11)
                                                                                                                       ggAACAGCA‑ACAGGCTAATGCGGAACCAGAAGACCAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAg                                 <  2:1318523/149‑1 (MQ=255)
                                                                                                                       ggAACACCA‑ACAGGATACTCCGGAAGCAGCAACAGTTGGCGATCCACGGAAAGCGGCCGTCGAAGCAGCTATTGCCCGTGCCAAAG‑CCCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgc                                <  3:101428/150‑1 (MQ=11)
                                                                                                                          aacatcttcaggaaaaTGCGGAAGCAGAATCACTGGTTGATCCTCGAAAAGCCGCAGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgc                             <  6:886231/137‑1 (MQ=14)
                                                                                                                            aGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCGGCAGTGGACGCGGCTATTGCCCGCGCGCACG‑CCCGCAACCGGGAACAGCACCAGGCTTATGACGAGCCATAACTGCAGGTCGCTCTCCGCACAGCCGCAGt                          >  3:1294630/1‑151 (MQ=9)
                                                                                                                                  caggataattacgAACCAGAATAAGTTGTAGATCCTCGTAAATCTGCCGTTGAAGCAGCTATTGCCCGTGCCAAAG‑CTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGaa                      <  1:1803852/138‑1 (MQ=11)
                                                                                                                                   aGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCGGCTAt                                                                                                               >  2:1741106/1‑61 (MQ=255)
                                                                                                                                        aaTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCGGCAGTCGACGCGGCTATTGCCCGAGCCAACG‑CAAGAAACCAGGCACAGCAGAAGGGTAATGACGAGCCAGAACTCCACGTCGATACGCGCAGAGCCGCCGTCTCAGCGGCtt               >  2:1303687/1‑150 (MQ=9)
                                                                                                                                        aaTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCGGCTATTGCCCGAGCAAAAG‑CAAGCAAGCTGGAACAGCAACAGACTAATCCCGTCCCAGCACCACAGGTCGCTACGCGCATAGCCTCCCTGGAAGCGGCtt               >  7:1781088/1‑150 (MQ=14)
                                                                                                                                         aTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAACG‑CACGAAAGCGGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCCCCCACAGCCGCAGTTGACGAGGCTAt              >  3:1244669/1‑151 (MQ=14)
                                                                                                                                          tGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCACGAGCCAAAG‑CACGCAAGCTGGAACAACAACCGAATAAAGCCTATACAGAAAAACAGTACGATACGCTCAAAAACTCCGTCGAAGCCGATaa              >  5:942867/1‑149 (MQ=2)
                                                                                                                                           tctgacccAGAACAAGAGGTCGATCCTCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAtt             <  4:1314188/144‑1 (MQ=11)
                                                                                                                                            cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAG‑CCCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGCTGCGCGCGAGGCCGCGGGCGAAGCCGCGAGTGc           >  8:1785442/1‑151 (MQ=14)
                                                                                                                                             cgAAGCAGAACAACAGGCTGATCCTCGAAAACCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGcc          <  8:1471595/150‑1 (MQ=11)
                                                                                                                                               aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCg        <  2:381485/151‑1 (MQ=25)
                                                                                                                                                aCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCGAAGG‑CAAGAAAGCGCGCACAGCACCAGGGTGATGCAGATCCAGAGCCACAGCGTGATCCGGCCACAGCCCCCGAGGAAGCGGCTATTGccggc       >  8:1222208/1‑148 (MQ=2)
                                                                                                                                                aCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGGGCCAAAG‑CCCACAACCAGGCCCAGCAACACGCTAATGCCCACGCAGGACAACAGGTCCAAACCCGCAAAGCCGCAGTCGCAGCGGGTATTGCACg        >  3:817828/1‑150 (MQ=2)
                                                                                                                                                   agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGCGcc    >  2:106468/1‑151 (MQ=255)
                                                                                                                                                     aagaaCAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGAGCCAAAG‑CACGCAAGCTCGAACAGCAACAGGCTAATGCCGAGCCAGAACAACACGTGGATACGCGCAAAGCCGCAGTAGCAGCGGCTATTGCCCgcgcgc   >  4:1412511/1‑148 (MQ=14)
                                                                                                                                                       |                                                                                                                                                     
CCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTG‑CCAAAGCGC‑GCAAGCTGGAACAGCA‑ACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAA  >  NC_000913/1708610‑1708906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑