Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1450TC24.3% 343.0 / 126.7 220intergenic (‑10/+53)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (55/88);  new base C (5/41);  total (86/134)
Fisher's exact test for biased strand distribution p-value = 4.34e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.31e-04

Only 100 of 253 total aligned reads displayed.

AGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTG  >  NC_000913/2817997‑2818295
                                                                                                                                                     |                                                                                                                                                         
aGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAattt                                                                                                                                                         <  1:13089/151‑1 (MQ=40)
aGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAat                                                                                                                                                           <  8:1506191/149‑1 (MQ=40)
   gCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatttatt                                                                                                                                                      <  3:958122/151‑1 (MQ=255)
   gCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatttatt                                                                                                                                                      <  7:518481/151‑1 (MQ=255)
    gcTGCGTTTATTTCCTCTACCTCTTCCATACCTTCATTGCTGAATATTTCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAacttactt                                                                                                                                                     <  5:770027/150‑1 (MQ=14)
    ccTGCGGTTATTTCCGCTACCTCTTCTTTACCTTCATTTCTGAAGATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatttattt                                                                                                                                                     <  6:1151139/151‑1 (MQ=255)
      tGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTActtt                                                                                                                                                    >  2:741513/1‑150 (MQ=255)
       gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCAGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAtttt                                                                                                                                                    <  8:332545/149‑1 (MQ=255)
         ggTTAATACCGCTAGCTCTTCGATACCTTCATTGCTGAAGCTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActg                                                                                                                                                <  8:1355364/151‑1 (MQ=255)
            tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                              <  5:1734444/150‑1 (MQ=255)
             aaTTCCTCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTActgcag                                                                                                                                             <  6:1209771/150‑3 (MQ=255)
             aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTActgctg                                                                                                                                             >  1:572527/1‑150 (MQ=255)
              aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTActgc                                                                                                                                               >  7:1573102/1‑147 (MQ=255)
               ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGa‑‑tt                                                                                                                                        >  4:84398/1‑150 (MQ=255)
                tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                       >  2:485972/1‑151 (MQ=255)
                tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑ta                                                                                                                                        <  7:1512360/150‑1 (MQ=255)
                tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑ta                                                                                                                                        <  8:169419/150‑1 (MQ=255)
                 ccGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                              >  8:1419469/1‑145 (MQ=9)
                 actcTACATATTCTATACGTTAATACCTGAATATTTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑ttt                                                                                                                                       <  5:188863/147‑3 (MQ=1)
                  cGCTACCTCATCGATACCCTCAATCCTTAATTTTTATTAGAATATGGCGCATCCGGTATGATTCCAACAACCGACCTCTCTTTTCGTACACGAGTACTTTATCATGTTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTg                                                                                                                                     <  4:1320971/151‑1 (MQ=9)
                   gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTACAACCAAAGACCCAACCGATACTAGCCGATTAGTCTAACAAGATAATCTACGGAAGCAACTCG‑AAAATTAAATTACTGAAGA‑‑TTTTgt                                                                                                                                    >  6:581470/1‑150 (MQ=2)
                     tACCTCTTCGATACCTTCATTGCTGAAGATATCGCACAATATGGAGCATCCGCGAGCATTCGAACCTCCCACCGCACGCTACCTACCCGACTACTCTATCCAGCTCACCTACCGATCCATCGAC‑AAAATTAATTTActccacaaaataat                                                                                                                                     >  5:327943/1‑138 (MQ=9)
                      agatattgtttACGTTAATTGATGAATATTTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                                    <  6:1460213/138‑3 (MQ=11)
                       ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTACTTTACTGCAGA‑‑ttttttaga                                                                                                                                 >  4:1184328/1‑145 (MQ=14)
                       ccTCTTCGATACATTGATTTCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATACATCTTAGCTACGGATGCATCGGT‑AAATTTATTTTACTTCTGA‑‑TATTGATAcc                                                                                                                                <  5:1155077/151‑1 (MQ=14)
                       catctTGGATACCCTCATTCCGGAAGATTTCGGACTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttgata                                                                                                                                <  1:924497/149‑7 (MQ=1)
                           ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTACTTTACTGCAGATTTTTTGATACCGc                                                                                                                              >  8:1005538/1‑151 (MQ=14)
                            tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGct                                                                                                                             <  4:959460/149‑1 (MQ=255)
                             cgcacccTTCATTCCTCAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTAATACCGCTCGGTTCGTAGCCTATTACTCTATCCAGCTTAGCTACTTATGCATCTGG‑AACTTTATTTTACTTCTGA‑‑TATTGATACCGctact                                                                                                                          <  5:1467032/146‑1 (MQ=14)
                             cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGctac                                                                                                                           <  8:640647/150‑1 (MQ=255)
                              tatacgttactttcTGAAGATTTAGTATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGcta                                                                                                                            <  6:272666/137‑1 (MQ=14)
                              gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TTTTtatagagcgata                                                                                                                          >  3:439692/1‑142 (MQ=14)
                               atacgatcttttcTGAATATTTCGGATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGctac                                                                                                                           <  5:1199206/138‑1 (MQ=14)
                               aaaccataatagatGAATATGTCGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGctact                                                                                                                          <  3:349534/138‑1 (MQ=14)
                               aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTaa                                                                                                                        <  3:912424/151‑1 (MQ=255)
                               aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCAGA‑‑TAtttttaacgaacgta                                                                                                                         >  8:1197753/1‑137 (MQ=14)
                               aTACCTTCATTGCTGAAGATTTCGCAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGTTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTaa                                                                                                                        <  7:732811/151‑1 (MQ=255)
                                tACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATAGAGCTAGTaa                                                                                                                        >  7:1766863/1‑150 (MQ=255)
                                tACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCAGA‑‑tttttagatacctaaaaa                                                                                                                        >  1:909676/1‑136 (MQ=11)
                                agagctggtatcttcaGATCTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGctacta                                                                                                                         <  2:257064/136‑1 (MQ=14)
                                 aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTaaa                                                                                                                       <  3:1741209/150‑1 (MQ=255)
                                   gttaattcatGAATATTTCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAg                                                                                                                      <  1:1713706/142‑1 (MQ=11)
                                      catatatGAATATTTAGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGcc                                                                                                                    <  2:1135534/144‑1 (MQ=14)
                                      cATTGCTGAATATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑tt                                                                                                                                        <  8:1253291/128‑2 (MQ=11)
                                       aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGATGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCatat                                                                                                                <  2:1100072/151‑1 (MQ=255)
                                         tGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATc                                                                                                               <  1:108541/150‑1 (MQ=255)
                                          gCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑t                                                                                                                                         >  3:177435/1‑123 (MQ=18)
                                          gCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑t                                                                                                                                         <  5:177231/123‑1 (MQ=18)
                                          gCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCa                                                                                                              <  3:266677/150‑1 (MQ=255)
                                             gAATATTTAGTAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAATTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAg                                                                                                            <  5:463194/151‑1 (MQ=14)
                                             caatatttagtattatatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCaa                                                                                                             <  5:695965/136‑1 (MQ=17)
                                              aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTaa                                                                                                         <  8:481787/151‑1 (MQ=255)
                                              aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTaa                                                                                                         <  2:663177/151‑1 (MQ=255)
                                              aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTaa                                                                                                         <  5:416086/151‑1 (MQ=255)
                                               aGATTTCGGAGAATATGGTGCATCTGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAATTTACTGCTGA‑‑TATTTATAAACCTAATAAATACATATaaacaaa                                                                                                         >  1:34498/1‑146 (MQ=14)
                                                     cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActg                                                                                                                                                >  7:270931/1‑107 (MQ=40)
                                                     cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActg                                                                                                                                                <  1:270450/107‑1 (MQ=40)
                                                     cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATgg                                                                                                  <  4:662863/151‑1 (MQ=39)
                                                     cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATg                                                                                                   <  2:328972/150‑1 (MQ=40)
                                                     cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAATTTACTGCTGA‑‑TATTGAGAGAGCTAAAAAAGCCagatatactataagatct                                                                                                  >  7:1307513/1‑137 (MQ=11)
                                                      ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTa                                                                                                                                                   <  5:1021114/103‑1 (MQ=39)
                                                      ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTa                                                                                                                                                   >  3:1054330/1‑103 (MQ=39)
                                                         gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTAc                                                                                                                                                  >  3:1720935/1‑101 (MQ=40)
                                                         gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCa                                                                                              <  3:64266/151‑1 (MQ=39)
                                                          aaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                       <  5:44115/109‑1 (MQ=40)
                                                          aaTATGGTGCATCCGGGAGGATTAGAACATCCGAACCCACTCTTCGTAGCAGAGTAATGTATAAAGCTGAGATAAGGATGCATCGGG‑AAAATTATTTTAATAAAGA‑‑TATTGATAGCGCTAGTATAGCGAGGTCTAGTGTGAGATGGTgggg                                                                                             >  4:585423/1‑148 (MQ=2)
                                                                  gCATCCGGGAGGATTCGAACCTCAGACCGCTCGGTTAGTAGCCGAGTACTCTCTCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAGTTTACTGATGA‑‑TATTTAAAGAACTAAAAAAGCCAGGTCTAGTAAGAGATGGTGCGGCGGggagg                                                                                     >  4:1813969/1‑151 (MQ=11)
                                                                    aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGAt                                                                                   >  8:1800914/1‑151 (MQ=39)
                                                                     tCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACAGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGAtt                                                                                  >  7:331594/1‑151 (MQ=255)
                                                                       cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTCCTAAAGCGCTATCAAGTAAGAGATGGTGCGGCCGGGAGGCttat                                                                                >  3:49179/1‑149 (MQ=14)
                                                                           aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTTAtt                                                                                                                                  >  5:910363/1‑96 (MQ=255)
                                                                           aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACACCTACTAAACCCAGATCTAGTAAGAGATCGTGGATCACGGAGGATTAGacgc                                                                            >  2:1774064/1‑148 (MQ=14)
                                                                              aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑TATTGATACAGCTACTAAAGCCATATCAAGTAAGAGATGTTGCCTCAGCGAGGATTAGacgcgac                                                                         >  7:551059/1‑145 (MQ=14)
                                                                                 cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCAGATCAAGTAAGAGATGGTGGATCGCGGAAGAtccccacggcccac                                                                      >  7:1248877/1‑138 (MQ=11)
                                                                                  ccaCCTCCGCCCGCGCGCGGCGTAGCCGAGTACTCTATCCACCTCAGCTACCTACGCATCGGG‑AAATTTATTTTACTCCTGA‑‑TATTGAAACCGCTACTAAAGCAATATCAAGTACCATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑cc                                                                    <  2:1651011/149‑1 (MQ=255)
                                                                                   aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActg                                                                                                                                                <  3:1146323/77‑1 (MQ=38)
                                                                                     ccTCGGACCGGTCGGTTCGTACCATAGTCCTCTATACCGATCATGTTCCTACGCATCGGG‑AAATTTATATTACTGCTGA‑‑TATTGATACCGCTACTACCGCCATATCAAGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCt                                                                 <  2:1398683/151‑1 (MQ=12)
                                                                                      cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTa                                                                                                                                                   >  3:1455266/1‑71 (MQ=38)
                                                                                         cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATAAAGCTAATAAAAACATATAAAATAAAAGATCTTGAATAAGCGAGGATTAGAAACGCCGA‑cccgagcg                                                              >  5:3692/1‑144 (MQ=14)
                                                                                            ccGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑ATATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCAGGGAGGATTAGAAGCTCCGA‑GCggatcgctcg                                                          >  2:1309976/1‑142 (MQ=14)
                                                                                               cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAGCCTCCGA‑CCCCTCGGGTcctcc                                                       >  2:1488911/1‑147 (MQ=255)
                                                                                                     gctgtgCCGAGGAGTCTAACCCGCTGCGCAACCGAGGCATCGGC‑ACAGTTATTTTACTCCAGA‑‑TCTTGCAACCCGTCATACAGGCATATCACGTACGATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGt                                                 <  3:460467/146‑1 (MQ=9)
                                                                                                     ccGTAGCCCATCACACTATCCCGCTCCGCTACCGATCCATCGGC‑AAATTTATTTTCCTCCTGA‑‑TCTTGCAACCGCTACTACATCCATATCACGTACTATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGt                                                 <  3:469119/150‑1 (MQ=12)
                                                                                                         cgCCGAGTACTCTATCCTGCTCCGATACGCATCCATCGTG‑ATCTTTATCTTACTGCTCA‑‑TCTTGTACCCTCAACTACCGCCAGATCTAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTActc                                             <  6:1483628/150‑1 (MQ=12)
                                                                                                            cGAGTACTCTATCAAGCTGCGCTACGGAGGCATCGGG‑AAATTTAATTTACTGTTGA‑‑TATTGATACCGCTACTACAGCCATATATAGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTAt                                          <  2:988019/151‑1 (MQ=21)
                                                                                                               gTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGACCCTCCGA‑CCGCTCGGTTCGGAGCCGatgagaataccac                                       >  2:1168649/1‑139 (MQ=18)
                                                                                                                tACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TTTTTATACCGCTAATAAAGCCATATCAAGTAAGAGATGGTGAATCAGGGAGGATAAGACTCGCCGAGCCGCTC‑GCTCGTCGCCGAGTagaaaaacaa                                       >  6:1572049/1‑141 (MQ=14)
                                                                                                                aaCTCTATCCATCTGAGCTACGGAGGAAGCGGC‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                      <  2:401201/150‑1 (MQ=255)
                                                                                                                                 atacttctccATCGGC‑TAATTTAAATTACTCCTCC‑‑TCTTGTTACCGATACTACCGGAATATTAAGTAATAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCa                      <  4:298705/141‑1 (MQ=14)
                                                                                                                                     ggATTCATCGGGAAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGa                                                                               <  6:1732389/91‑1 (MQ=255)
                                                                                                                                     ggATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCggg                 >  6:452256/1‑151 (MQ=255)
                                                                                                                                       aTGCATCGGGAAAACTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTa                                                        <  2:928028/111‑1 (MQ=255)
                                                                                                                                             cGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaatggcggtgagg         >  3:1651172/1‑138 (MQ=255)
                                                                                                                                             cGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCAGA‑CCCATCCGATCGTAGCCGAGTAATCTATCCAGATGAGATACGGATGCATAGGGAAaatta          >  4:1627901/1‑150 (MQ=21)
                                                                                                                                              ggg‑ATATTTATTTTACTGCTTA‑‑TATTGATACAGCTACAAAAGCCATATCAAGTACTAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaatggcggtgaggc        <  5:1648408/151‑15 (MQ=17)
                                                                                                                                              ccg‑AAATTTATTTTCTTGCTCT‑‑TTTTGATACCCGTAATTAATCCATATCAAGTATGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttac         <  1:1412845/148‑1 (MQ=17)
                                                                                                                                                  aaaCTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTACGA‑CCgaaccga                                                             >  1:1499529/1‑89 (MQ=11)
                                                                                                                                                   aaTTTTTTTTACTGCACA‑‑TCTTGTAACCCGTCCTATCTCCATATCTAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttactttac    <  8:1773488/151‑1 (MQ=14)
                                                                                                                                                    atttatttTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaa                     >  6:657598/1‑132 (MQ=255)
                                                                                                                                                     tttatttTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTAAGA‑CCCGACGGTTCGTAGCCGATTAGTCTATCCAGATGAGCTACGGATGCATAGGGAAAAttactttactc  >  4:405528/1‑150 (MQ=21)
                                                                                                                                                     |                                                                                                                                                         
AGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTG  >  NC_000913/2817997‑2818295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑