Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1580TA25.1% 331.2 / 107.2 200intergenic (‑23/+40)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (61/88);  new base A (12/38);  total (74/126)
Fisher's exact test for biased strand distribution p-value = 4.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGAT  >  NC_000913/2818009‑2818308
                                                                                                                                                      |                                                                                                                                                           
 atttCCTCTAGATCTTCCATACCCTACTTCATCAAGATTACGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa                                                                                                                                                           <  1:1439319/149‑1 (MQ=11)
 aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTActgctg                                                                                                                                                            >  1:572527/1‑150 (MQ=255)
  attactatACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATTGTGCATCCGGGAGGATTCGAACCTCCTAACGCTCTGTTCGTATCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑t                                                                                                                                                        <  5:1389573/144‑1 (MQ=14)
   ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGa‑‑tt                                                                                                                                                       >  4:84398/1‑150 (MQ=255)
    tCCGCTACCTCTTCGATTCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑ta                                                                                                                                                       <  7:595011/150‑1 (MQ=255)
     ccGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                                             >  8:1419469/1‑145 (MQ=9)
     actcTACATATTCTATACGTTAATACCTGAATATTTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑ttt                                                                                                                                                      <  5:188863/147‑3 (MQ=1)
      cGCTACCTCATCGATACCCTCAATCCTTAATTTTTATTAGAATATGGCGCATCCGGTATGATTCCAACAACCGACCTCTCTTTTCGTACACGAGTACTTTATCATGTTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑g                                                                                                                                                  <  4:1320971/151‑1 (MQ=9)
         tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCAGA‑‑ttttttg                                                                                                                                                  >  3:481191/1‑147 (MQ=255)
          agatattgtttACGTTAATTGATGAATATTTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                                                   <  6:1460213/138‑3 (MQ=11)
          acatctTCCAAACCTTCATACCTGAAGATTTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttg                                                                                                                                                  <  5:345858/146‑4 (MQ=11)
          aCCTCTTCTATCCCTTCATTGCTGCAGATTTAGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttg                                                                                                                                                  <  1:1570922/149‑4 (MQ=11)
           tcttttCTATCCCTTCCTTCCGGCAGATTTCGTACTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttga                                                                                                                                                 <  6:1195497/145‑5 (MQ=11)
           gatattCCATACCTTCATTCATCAATATATCGTATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                                                   <  2:1186404/143‑3 (MQ=11)
           ccTCTTCGATACCTTCCTTGCTGAAGATTTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttgata                                                                                                                                               <  3:183446/151‑7 (MQ=14)
           ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTACTTTACTGCAGA‑‑ttttttaga                                                                                                                                                >  4:1184328/1‑145 (MQ=14)
           ccTCTTCGATACATTGATTTCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATACATCTTAGCTACGGATGCATCGGT‑AAATTTATTTTACTTCTGA‑‑TATT‑‑GATAcc                                                                                                                                             <  5:1155077/151‑1 (MQ=14)
              ctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                                             >  6:1711340/1‑136 (MQ=18)
              ctTCGATACCTTCATTGCTGAAGCTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCg                                                                                                                                            <  3:166609/149‑1 (MQ=255)
              ctTCGATACCTTCATTGCTGAAGCTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                                             <  8:1714497/136‑1 (MQ=25)
              ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGCTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCg                                                                                                                                            <  1:301968/149‑1 (MQ=255)
               ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCAGA‑‑TAtttttatacct                                                                                                                                            >  1:1297350/1‑141 (MQ=14)
               ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTACTTTACTGCAGATTTTTT‑‑GATACCGc                                                                                                                                           >  8:1005538/1‑151 (MQ=14)
                tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGct                                                                                                                                          <  4:959460/149‑1 (MQ=255)
                tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCAGA‑‑TATT‑‑GATAAAGcta                                                                                                                                         >  2:1798480/1‑150 (MQ=255)
                 cgcacccTTCATTCCTCAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTAATACCGCTCGGTTCGTAGCCTATTACTCTATCCAGCTTAGCTACTTATGCATCTGG‑AACTTTATTTTACTTCTGA‑‑TATT‑‑GATACCGctact                                                                                                                                       <  5:1467032/146‑1 (MQ=14)
                 cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGctact                                                                                                                                       <  7:1030127/151‑1 (MQ=255)
                   taacgataaTTGCGGAAGATGTAGTATCATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGctac                                                                                                                                        <  7:134452/142‑1 (MQ=14)
                   atacgatcttttcTGAATATTTCGGATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGctac                                                                                                                                        <  5:1199206/138‑1 (MQ=14)
                   aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTaa                                                                                                                                     <  3:912424/151‑1 (MQ=255)
                   aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCAGA‑‑TAtttttaacgaacgta                                                                                                                                        >  8:1197753/1‑137 (MQ=14)
                   aTACCTTCATTGCTGAAGATTTCGCAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGTTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTaa                                                                                                                                     <  7:732811/151‑1 (MQ=255)
                    tccgttaaTTGCTGAATATTTAGTAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGctact                                                                                                                                       <  4:383535/143‑1 (MQ=14)
                    tACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATAGAGCTAGTaa                                                                                                                                     >  7:1766863/1‑150 (MQ=255)
                    tACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TATT‑‑TAGACCGCTACTaa                                                                                                                                     >  3:437685/1‑150 (MQ=255)
                     aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTaaa                                                                                                                                    <  3:1618409/150‑1 (MQ=255)
                      ccTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACAGCTCGGTTCGTAGCCTAGTACTCTATCAAGCAGATCTACGGATGCATAGAG‑AAAATTAATTTACTGCTGA‑‑TATT‑‑GATAGCGCTAATAAAGc                                                                                                                                  >  4:658968/1‑151 (MQ=11)
                          cATTTCTCAATATTTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGCTACTAAAGcc                                                                                                                                 <  2:1154529/150‑1 (MQ=14)
                             tGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCTAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATc                                                                                                                            <  1:91257/150‑1 (MQ=255)
                              tcgccaGATTTCGGACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGCTACTAAAGCCgtat                                                                                                                             <  5:1783667/145‑1 (MQ=14)
                              gCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCa                                                                                                                           <  3:266677/150‑1 (MQ=255)
                              gCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TTTTTAGAGACCGCGTCTAAAGCAataa                                                                                                                             >  8:1518965/1‑149 (MQ=14)
                                 gaatatctcgtattatatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGCTACTAAAGCCGTATCa                                                                                                                           <  6:222732/135‑1 (MQ=17)
                                 gAATATTTAGTAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAATTTACTGCAGATTTTTT‑‑GATACCGCTACTAAAGCCGTATCAAg                                                                                                                         <  5:463194/151‑1 (MQ=14)
                                 caatatttagtattatatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGCTACTAAAGCCGTATCaa                                                                                                                          <  5:695965/136‑1 (MQ=17)
                                  aatatttattattatatTGTGCTTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACTGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGCTACTAAAGCCGTATCAAGt                                                                                                                        <  5:436952/138‑1 (MQ=14)
                                  aaGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCGCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGT‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTa                                                                                                                       <  3:77206/150‑1 (MQ=255)
                                  aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTaa                                                                                                                      <  2:663177/151‑1 (MQ=255)
                                   aGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAag                                                                                                                     <  3:1751004/151‑1 (MQ=255)
                                     gttacGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa                                                                                                                                                           >  2:435409/5‑115 (MQ=25)
                                     gttacGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa                                                                                                                                                           <  4:434662/111‑1 (MQ=25)
                                         cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATg                                                                                                                <  2:328972/150‑1 (MQ=40)
                                         cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAATTTACTGCTGA‑‑TATT‑‑GAGAGAGCTAAAAAAGCCagatatactataagatct                                                                                                               >  7:1307513/1‑137 (MQ=11)
                                              aaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                                      >  3:44254/1‑109 (MQ=40)
                                                        aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATGTCAACTTAGAGATGGTGCATCCCGGAGGAt                                                                                                >  2:1322054/1‑151 (MQ=255)
                                                        aTCCGGGAGGATTCGAACCTCCGACCGCGCGGTTCGTAGCCGAGTACTCTATCCCGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGAt                                                                                                <  2:1108885/151‑1 (MQ=255)
                                                          ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TATT‑‑TATACCGCTACTATAGCGATATCAATTAAGAGATCGTGCTTCCGggagcagtc                                                                                              >  2:944318/1‑146 (MQ=11)
                                                            gggAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                                     >  7:281045/1‑96 (MQ=255)
                                                            gggAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGAtt                                                                                               >  2:895514/1‑148 (MQ=40)
                                                              gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCa                                                                                                                           <  7:1817360/118‑1 (MQ=40)
                                                               aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑TAtt                                                                                                                                               >  5:910363/1‑96 (MQ=255)
                                                                  aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTAGTATAGCCATATCAAGTAAGAGATGGTGCATCCGCGAGGATTAGacgcgcc                                                                                      >  7:1106699/1‑145 (MQ=11)
                                                                         ccTCGGACCGGTCGGTTCGTACCATAGTCCTCTATACCGATCATGTTCCTACGCATCGGG‑AAATTTATATTACTGCTGA‑‑TATT‑‑GATACCGCTACTACCGCCATATCAAGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCt                                                                              <  2:1398683/151‑1 (MQ=12)
                                                                           tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTAGTAAAGCCATATCAAGTAATAGATGGTGCATCCGGGAGGATTAGactcgccgagcgtctc                                                                             >  8:191458/1‑136 (MQ=14)
                                                                            ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑TATACCGCTACTAAAGCCATATAAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCTCAGAGCCGCTCg                                                                            >  7:1519554/1‑151 (MQ=14)
                                                                             cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAACTAAGAGATGGTGCATCCGGGAGGATTAGACCCGCAGA‑CCgtcgcgc                                                                          >  2:1682905/1‑145 (MQ=11)
                                                                              gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TATT‑‑TATAACGCTAATAAAGACAAATAAAATAAAAGATCTTGCATCACCGAGGATTAGACTCGCCGAGCCGCTCga                                                                           >  1:955230/1‑149 (MQ=2)
                                                                                         gcGTAGCCGAGTACTCTATCCCCCTCGGCTACCGCTCCATCGGG‑AAATTTATATTACTGCTGA‑‑TATT‑‑GCTACCGCTCCTACCGCCATATCTAGGACTAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGt                                                              <  2:1758431/150‑1 (MQ=18)
                                                                                         ccGTAGCCCATCACACTATCCCGCTCCGCTACCGATCCATCGGC‑AAATTTATTTTCCTCCTGA‑‑TCTT‑‑GCAACCGCTACTACATCCATATCACGTACTATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGt                                                              <  3:469119/150‑1 (MQ=12)
                                                                                                cGAGTACTCTATCAAGCTGCGCTACGGAGGCATCGGG‑AAATTTAATTTACTGTTGA‑‑TATT‑‑GATACCGCTACTACAGCCATATATAGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTAt                                                       <  2:988019/151‑1 (MQ=21)
                                                                                                    aaCTCTATCCATCTGAGCTACGGAGGAAGCGGC‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                   <  2:401201/150‑1 (MQ=255)
                                                                                                       tctATCCTGATGAGCTACGTATGGATCTGG‑ATATTTATTTTACTTTTGA‑‑TATT‑‑GTTACCGCTACTAATGCCATATGAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                 <  4:706653/150‑1 (MQ=21)
                                                                                                        ctATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTACACGCGCGGA‑CCGGCGGGATCGTCGCCGAGTAGAATAACCAGCTgt                                               >  8:526816/1‑150 (MQ=14)
                                                                                                          atttataatagatACGGCTCCATCGGT‑AAATTTATCTTAATCCTCC‑‑TCTT‑‑TTTCCCTCTACTAATCGCTTATGATGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                <  6:612285/136‑1 (MQ=0)
                                                                                                               gCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACAGCTACTAAAGCCATATCAAGTAAGAGATAGTGAATAAGGGAGGATTCGAACCTCCGA‑CCGGACGGATCGTAGCCGAGTAGTCTATCCAGCTGACCTACgg                                        >  1:839351/1‑151 (MQ=21)
                                                                                                                 ggAGCTACGGATGCAGCGGC‑AAAGTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGAt                                      <  2:1030761/150‑1 (MQ=255)
                                                                                                                  gctcttccgATGCAGCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GTTTACGCTACTACAGCCATATCAAGTATGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGAt                                      <  6:577811/142‑1 (MQ=21)
                                                                                                                     caaCGGATCCATCGGC‑TCATGTTTTTTACTGATGA‑‑TATT‑‑CCTTACCTTAATACATCTATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCa                                   <  7:1608390/148‑1 (MQ=14)
                                                                                                                      tACTGATGCAGCGGG‑ACATTTAATTTACTGCTGA‑‑TCTT‑‑GAAACCGCTACTACAGGAATATGACGTAATATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAt                                  <  2:1570433/150‑1 (MQ=17)
                                                                                                                       aCGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaat                                >  4:1746376/1‑148 (MQ=255)
                                                                                                                         ggattgaTCGGGTAAAGTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGc                                    <  1:1325615/140‑1 (MQ=255)
                                                                                                                         ggATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCggg                              >  6:452256/1‑151 (MQ=255)
                                                                                                                         cgtagccagcggctcatttatatTCCTGTTCA‑‑TCTT‑‑GTTAACCCTACTTCCGCAAGATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCgg                               <  3:527145/136‑1 (MQ=14)
                                                                                                                            tGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGACCCTGAGA‑CCCGACCGTTCGTAGCCGATTAGAATCAACAGCTGACATACGGATGCATcacatg                            >  7:4598/1‑146 (MQ=2)
                                                                                                                             gCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCCTATCAAGTAAGAGATGGTGAAACCGGGGGGATGCGAAACTCGGA‑CCGATCGGGTCGAACGCGAGGACGCGCGCCAGCTGAGAAAACGAAGCAGCGGGaagc                          >  5:783546/1‑149 (MQ=12)
                                                                                                                              ccttttttaaaTTTATTATACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaa                           <  5:395968/142‑1 (MQ=255)
                                                                                                                              cATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGATCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaatggcggat                         >  5:1730192/1‑141 (MQ=255)
                                                                                                                                     aaaaCTTATTCTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑cccatc                                                                             >  3:1738182/2‑89 (MQ=11)
                                                                                                                                     aaaaCTTATTCTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTc                                                                             <  5:1735419/92‑1 (MQ=255)
                                                                                                                                       aaTTTTTTTTACTGCACA‑‑TCTT‑‑GTAACCCGTCCTATCTCCATATCTAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttactttac                 <  8:1773488/151‑1 (MQ=14)
                                                                                                                                        atttatttTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaa                                  >  4:1825626/1‑132 (MQ=255)
                                                                                                                                          ttatttTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTg               <  8:452836/150‑1 (MQ=255)
                                                                                                                                                tACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                 <  8:1125082/110‑1 (MQ=255)
                                                                                                                                                tACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAg            <  6:1502902/147‑1 (MQ=255)
                                                                                                                                                 aCTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTg                                                                                                             >  1:196602/1‑50 (MQ=35)
                                                                                                                                                 aCTGCTGA‑‑TATT‑‑GATAACGCTACTAAAGCAATATCAATTAATAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAtttt       <  4:1513492/151‑1 (MQ=255)
                                                                                                                                                  ctgctgA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCg                                                                                     <  1:1250806/73‑1 (MQ=255)
                                                                                                                                                  ctgctgA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTaa                                                                                                                      <  1:1076714/40‑1 (MQ=38)
                                                                                                                                                      tgA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCCACCCTGGGA‑CCCAACGGTTCGTCGCCGAGTCCTCTATCCAGGTGAGCTCCGTATGCAGAGGGCATATTACGTAACGGCAGACTTTTGGAt  >  7:546771/1‑151 (MQ=9)
                                                                                                                                                      tgA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTcc                                                                                      <  3:1418777/68‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                           
TAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGAT  >  NC_000913/2818009‑2818308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑