Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9940GT24.4% 303.4 / 17.6 212intergenic (+392/+631)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base G (20/116);  new base T (1/43);  total (22/190)
Fisher's exact test for biased strand distribution p-value = 2.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

TGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTG  >  NC_000913/3269844‑3270135
                                                                                                                                                      |                                                                                                                                             
tGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTGATGTTTATGCCCCTACAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAGGTAAATCAGGTg                                                                                                                                               >  7:1764329/1‑151 (MQ=17)
 gAAACCCGCCGGACCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTg                                                                                                                                               <  6:1590066/150‑1 (MQ=255)
 gAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATATTTCTGCCCCTCCAAAAAAAAAAACCCCCCGAAGCGGGTTTTTACGTAAAACAGGTg                                                                                                                                               >  1:1240079/1‑150 (MQ=255)
  aaaCCCTCCGTATCGTTTTATTACTTACAAAATTTTATTCTGACCGATAAGCCGCTTTCTTTTGGGTATTGTGTCGTGGACAGTCATTCATCTTTCTGGCCCTCCAAAAGCAAAAACCCGCCGAATCGGGTTTTTACGTAAATCAGGTGa                                                                                                                                              <  4:435684/150‑1 (MQ=1)
  aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                                                             <  2:1190516/151‑1 (MQ=255)
  aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGATTTCTGTTGGGTATAGTGTAGTGGACAGTACGTAAGGGTTATGCCCCGGAAAAAGTAAAAAAAACACGAAGCGGGGTTTTACGTAAATCAGGTgcc                                                                                                                                             >  8:714585/1‑149 (MQ=255)
   aaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaaa                                                                                                                                            <  1:1468278/151‑1 (MQ=255)
     cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCTGGTTTTTACGTAAAACAGGTGAAACt                                                                                                                                          <  8:627671/151‑1 (MQ=255)
     cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACt                                                                                                                                          <  4:207912/151‑1 (MQ=255)
      ccgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTg                                                                                                                                         <  5:536626/151‑1 (MQ=255)
      ccgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTg                                                                                                                                         <  1:283214/151‑1 (MQ=255)
       cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                                                        <  8:1296909/151‑1 (MQ=255)
       cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                                                        <  7:838108/151‑1 (MQ=255)
        gccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc                                                                                                                                       <  5:279759/151‑1 (MQ=255)
         ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                                                      <  3:1479921/151‑1 (MQ=255)
         ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                                                      <  1:1790792/151‑1 (MQ=255)
         ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                                                      <  1:1666056/151‑1 (MQ=255)
          cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCg                                                                                                                                     <  5:183458/151‑1 (MQ=255)
          cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGACAAATTCTTTTGGTTATAGTGTCTTTGACAGTAATTAATGTTTATGAACAACCAAAAGTAAAAAACAGAAAAAAAAAGTTTTTACGTAAAACAGGTGAAACTGACCg                                                                                                                                     >  6:895909/1‑151 (MQ=255)
           gcaccGGTTTATTACGTACATCAGGTAAAACTTTTTTATAATCATATTTCTTTTTGGTATAGCTTCTTGGACTGTTATTCATTTTTCTTACACTACTATATTAATATCTCGCCGTATCGGTTTTTTACGTAAATCAGGTGAAACTGACCGa                                                                                                                                    <  6:1448911/147‑1 (MQ=9)
            gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAt                                                                                                                                   <  2:1266329/151‑1 (MQ=255)
            gAACGGTTTAATACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTTAAAACCCGCCGAAGCGGGTTTTTACGTACAACAGGTGAAACTGACCGAt                                                                                                                                   <  6:356034/151‑1 (MQ=255)
             aactttttATTACTTACATCATGTCATACTGACCGATAAGCGGCTTTGTTTTTTGTATTGTGTCGTGGAAAGTCATTCATCTTTATGCCCCTCAAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                                                                                  <  1:1366103/146‑1 (MQ=11)
             aaCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATa                                                                                                                                  <  7:1140937/151‑1 (MQ=255)
              aCGGTTTATTACGTACATCAGGTAAAACTGACCGATCAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                                                                 <  7:1404911/151‑1 (MQ=255)
              aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                                                                 <  4:166112/151‑1 (MQ=255)
              aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                                                                 <  4:991376/151‑1 (MQ=255)
              aCGGTTTATTACGTACATCACGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                                                                 <  6:237343/151‑1 (MQ=255)
                ggTTTTTTTCGTAAATCAGGTAAAACTGACCGATAATCCGCTTTCTTTTGTGTATAGTGTCGTGGTCAGTCATTCATCTTTCTGCCACTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGc                                                                                                                               <  5:902066/151‑1 (MQ=11)
                ggTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGc                                                                                                                               <  8:691122/151‑1 (MQ=255)
                 gTTTTTTACGTACATCAGGTAAAACTTACCGATAAGCCTATTTCTTTTGGTTATAGTGTCGTGGAAAGTCATTCATATTTCTGCCACTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                                                                                  <  4:1210302/147‑1 (MQ=11)
                 gTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTTTAGTGTCGTGGACAGTCTTTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGcc                                                                                                                              <  6:323928/151‑1 (MQ=255)
                   ttattaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCg                                                                                                                             <  1:1013964/150‑1 (MQ=255)
                   ttattaCGTACATAAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCCAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCg                                                                                                                             <  8:1061320/150‑1 (MQ=255)
                    tattaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACATTCATTAATATTTCTGGCCCACCAAAATAAAAAAAAAACCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAccacataggagg                                                                                                                            >  4:588938/1‑140 (MQ=14)
                       tacttacatatttttaaaCTGACCGTTATGCCGTTTTCTTTTGGTGTTTGTGTCGTGGAATGTGTTTCATATTTCTGCCCCTCAAAAAGTTTAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGcttt                                                                                                                         <  6:1565719/135‑1 (MQ=2)
                       taCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGcttt                                                                                                                         <  8:1486570/150‑1 (MQ=40)
                        aCGTAGATCAGGTAAAAATTTCCGATATGTCGCTTGCTTTTGTTTCTAGTGTCGTGGACAGTGATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttc                                                                                                                        <  3:1047828/150‑1 (MQ=11)
                        aCGTACATCATGTAAAAATGAGCGATAAGGCGATTTCTTTTGTGTATAGTGTCGTGGACAGTGATTCATCTTTATGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttct                                                                                                                       <  7:1509433/151‑1 (MQ=14)
                        aCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTGATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttct                                                                                                                       <  2:351882/151‑1 (MQ=255)
                         cGTACATAATTTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttctt                                                                                                                      <  6:1771188/151‑1 (MQ=255)
                            aCATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                                    <  2:943854/150‑1 (MQ=39)
                             cATCAGTTAACACTGAGCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCTTTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                                                  <  4:843779/151‑1 (MQ=255)
                                 aGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGGGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtat                                                                                                              <  3:840912/151‑1 (MQ=25)
                                  ggTAAAACTGACCGATAAGCCGCTTTCTTTTTGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                             <  8:1348087/151‑1 (MQ=25)
                                  ggTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGGGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                             <  8:379542/151‑1 (MQ=25)
                                     aaaaCTGACCGATAAGCCGCTTTCTTTTGGGTCGAGTGTCGGGGAAAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgtg                                                                                                          <  2:968932/151‑1 (MQ=255)
                                     aaaaATGACCGATAATCCGCTTTCTTTTTTGGATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCTGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAgtg                                                                                                          <  4:631412/151‑1 (MQ=11)
                                      acaCTGAGCGATAAGCCGCTTTCTTTTGGGTAAAGTGTCGTCGACAGGGCTTCATCTTTCTGGCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgtgt                                                                                                         <  7:55337/149‑1 (MQ=11)
                                         attagcgagatggtgCTTTCTGTTGTGTATCGTCTCGTCGAAAGCGTTTGATCTTTCTGCCACTCCAAAAGTTTAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGt                                                                                                      <  7:318197/137‑1 (MQ=11)
                                         atGACCGATAAGCAGCTTTCATTTGGCGATAGTGTCGGCGACAGTCATTCATCTATCTGCCACTCCAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGt                                                                                                      <  2:1773249/150‑1 (MQ=11)
                                            aCCGATATGCCGGATGCTTTTGCCTATCGTGTCGTGGACAGTGTTACATCTTTATGCCACTCCAAAAGTACAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGa                                                                                                   <  5:1374070/151‑1 (MQ=11)
                                            aCCGATAAGCCGCTTGCTTTAGGGTAGAGTCTCGGGGACAGTCAATCATCTTTCTGCCACTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTgg                                                                                                    <  7:1561965/150‑1 (MQ=11)
                                                atatttcGCTTTATTTTGGTTTTACTTTCTTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt                                                                                               <  6:56984/145‑1 (MQ=11)
                                                aTAAGCCGCTTTCTTTTGGGTATTGTGTCGTCGACAGTGTTTCATATTTCTGCCCCTCAAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGt                                                                                               <  4:578129/151‑1 (MQ=11)
                                                 aaaGCAGCTTTCTTTTGGCTATAGTGTCGGGGACAGTCATTCATCTATCTGCCCCTCAAAAAGTAAAAACCCGCCGAACCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtc                                                                                              <  4:1607030/150‑1 (MQ=11)
                                                  attgagCTTTCTTTTGAGTATAGTCTCGGGGAATGGGTGTCTTATATATCGCCCTCCAAAATCACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtca                                                                                             <  3:1582675/146‑1 (MQ=2)
                                                    gCCGCATGCTTTAGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCACTCCAAAAGTAAAAACCCGCCGCATCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcatt                                                                                           <  3:544125/151‑1 (MQ=11)
                                                              tttGCGTTTTGTGTCGGGGAAAGGGATTCATCTATATGGCCCTCAACAATTTTAAACCCGCCGTACCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCttt                                                                                    <  3:970762/148‑1 (MQ=11)
                                                              ttagttgatcgtgtCGTGGAAAGTGTTTCTTATTACTGCCACTCCAAAAGTATAAACCCGCCGTAGCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCt                                                                                  <  1:729862/140‑1 (MQ=11)
                                                                  ggTTTAGTGTCGGCGAAAGTGTTTGATCTTTCTGGCCCTCCAAAAGTATAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGccc                                                                              <  2:394681/150‑1 (MQ=11)
                                                                   tgttcttgtCGTGGAAAGGGAATTTTCTTTCGGGCACTCAAAAATTTTAAAACCGCCGAATCGGGTTTTTACGTACATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGcccc                                                                             <  1:98559/144‑1 (MQ=11)
                                                                     ataGTGTCGGGGACAGGCTATCCTCTATCTGCCCCTCCAAAAGGAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTCAAACTCACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTc                                                                           <  8:47736/150‑1 (MQ=11)
                                                                          gtcgtcGCCAGTCATTCCTCTTTCTGCCCCTCCAAAAGTACAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAg                                                                     <  2:341082/151‑1 (MQ=255)
                                                                              tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTACATCGGCATTCTCCCCCTCCCAAAGCaa                                                                  >  8:57058/1‑150 (MQ=11)
                                                                                gACAGTGATTTTTATATCTCCCACTCAAAAAGTACAAACCCGCCGAACCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa                                                               <  4:1532405/151‑1 (MQ=11)
                                                                                    gggcttcatATTTCTGCCACTCCAAAAGTAAAAACCCGCCGTATCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                           <  2:825343/147‑1 (MQ=11)
                                                                                             ataactcgcacTCCAAAATGAAAAACCCGCCGTACCGGTTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                  <  2:1680624/141‑1 (MQ=11)
                                                                                               aactcgcacTCAAAAATTATAAACCCGCCGAATCGTTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGt                                                <  4:430787/143‑1 (MQ=11)
                                                                                                   ggcacTCCACAAGCACAAACCCGCCGGACCGGGTTATTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                            <  3:359431/147‑1 (MQ=11)
                                                                                                   gCCACTCAAAAAGCTCAAACCCGCCGCACCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                            <  1:69688/151‑1 (MQ=11)
                                                                                                   cccacTCAAAAAGAACAAACCCGCCGCATCGGTTTTTTACGTAAAACACGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                            <  3:1280354/147‑1 (MQ=11)
                                                                                                     cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGGCGGTGGTCTTTGTGGCCCGCCAAAAGCAAAAACCCGCCGCCGCgggggttttc                                          >  2:546028/1‑144 (MQ=11)
                                                                                                         caacaattagaaaCCCGCCGCACCGGGTTTTTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                                       <  1:414028/140‑1 (MQ=11)
                                                                                                         caaaaattttaaaCCCGCCGTATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                        <  5:1164267/139‑1 (MQ=11)
                                                                                                          aacaatcttaaaCCCGCCGTATCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                     <  5:1629468/142‑1 (MQ=11)
                                                                                                            aaagctcaaaCCCGCCGAAGCGGTTTATTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAcc                                   <  6:76716/144‑1 (MQ=255)
                                                                                                            aaaTTAGAAACCCGCCGTACCGGTTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAcc                                   <  6:1262553/151‑1 (MQ=11)
                                                                                                             aattttaaaCCCGACGTATCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGTTATAGTGTCTTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACATAAACCa                                  <  5:747072/145‑1 (MQ=11)
                                                                                                             aattacaaaCCCGCCGCATCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAGAACCCGCCGAAGCGGGTTTTTACGTAAACCa                                  <  1:621096/145‑1 (MQ=11)
                                                                                                             aagcacaaaCCCGCCGTAACGGGTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCa                                  <  8:875767/145‑1 (MQ=255)
                                                                                                             aaGCAAAACCCCGCCGCACCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAACCCCGCCGAAGCGGGTTTTTACGTAAACCa                                  <  2:137270/151‑1 (MQ=255)
                                                                                                              agcacaaaCCCGCCGAACCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                 <  8:1538625/146‑1 (MQ=255)
                                                                                                               gTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAGCTGACCGATAAGCCGCTTTCTGTGGGGTAGAGGGTCGGGGCGAGGCGTTCATGGTGTGGGGGCGGCAAAAGCAAAAACCCGCGGCAGGGGGGGGTTACGTagaagagg                                >  3:521123/1‑144 (MQ=2)
                                                                                                                cacaaaCCCGCCGAATCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAgg                                <  4:309925/147‑1 (MQ=255)
                                                                                                                 acaaaCCCGCCGCAGCCTGTTTTTACGAACACCTCGCTAAACTGAGCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAgg                                <  7:416052/147‑1 (MQ=11)
                                                                                                                 acaaaCCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTg                              <  6:909462/149‑1 (MQ=255)
                                                                                                                    aaCCCGCCGAAGCGGTTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                           <  5:1563087/151‑1 (MQ=25)
                                                                                                                        cgccgcACCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa                       <  7:1242091/151‑1 (MQ=25)
                                                                                                                         gccgAATCGGTTTTTTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                      <  2:1060181/151‑1 (MQ=255)
                                                                                                                          ccgAATCTGTTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                     <  5:601757/151‑1 (MQ=255)
                                                                                                                             aaGCGGTTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAt                  <  4:770456/151‑1 (MQ=9)
                                                                                                                              accgttttattACGTAAAACAGGTGAAAGTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTGTTTCATATTTCTGCCACTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                 <  4:1555564/142‑1 (MQ=11)
                                                                                                                               acGGGTTTTTACGTAAAACTGGTGAAACTGACAGATAAGCCGGTTTCTTTTGGGTATAGTGTCGTGGACAGTGATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATaa                <  3:659899/150‑1 (MQ=255)
                                                                                                                                tttatttttACGTAAAACACGTAAAACTTAACGATAAGCATCATTCTTTTGGTAATAGTCGCTTTTACTTTCTTTTTTATATATGGCACTCAAAAATCATAAACCCGCCGAATCGGTTTTTTACGTAAATCAGGTGAAACTGACCGATaa                <  1:1146363/146‑1 (MQ=2)
                                                                                                                                cGGTTTATTACGTAGAACAGGTTAAACTGTCCTATAAGCCGCTTTCTTTTTTCTTTCGTGTCGTCGAAAGTCTTTCATCTTTCTGCCACTCAAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg               <  7:951176/151‑1 (MQ=11)
                                                                                                                                 gggTTTTTACTTAGAACATTTTATACTGACCGATATGCCGCTTTCTTTTGGGTATAGTGTCGTGGACTGTCAATCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc              <  8:1700374/151‑1 (MQ=11)
                                                                                                                                 gggTTATTACGTACAACAGGTCAAACTGACCTATAAGCCGCTTTCTTTTGGTTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTGCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc              <  2:636733/151‑1 (MQ=11)
                                                                                                                                  gttttttACTTACAACAGGTAAAACTGACAGATCAGCCGCTTTGTTTTGTGGATAGTCTCGTTGACAGTCATTCATCTTTCTCCCACTCAAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc             <  7:116747/149‑1 (MQ=11)
                                                                                                                                   ttttttAAGTAAATCATGTAAAATTTCCCTATAAGACGCTTTTTTTTTGTTTTTGTGTCGGGGAATGTGCTTTTTATTTATGGCACTCAAAAATCATAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGcc             <  1:790371/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                             
TGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTG  >  NC_000913/3269844‑3270135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑