BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,167	0	C	G	24.0%	358.6 / 44.8	262	intergenic (+565/+458)	yhaC/garK	pentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand): ref base C (96/62); new base G (50/0); total (192/70)
Fisher's exact test for biased strand distribution p-value = 1.63e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

ACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGC  >  NC_000913/3270038‑3270317

  aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGCGAGGCCTTCATGGTTCGGCCCCTGCAAAATCAAAAAccc                                                                                                                                    >  8:1409307/1‑151 (MQ=11)

   gTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGCGTCGTGGCTAGGCCTTCAATTTTTTGCGCCGACAAAAACAAAAAcccc                                                                                                                                   >  2:928801/1‑150 (MQ=11)

      attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAAAGCGTGGACGCGAGTCCATCATTATAAAGCGCCCTCAAAAACAAAAACccgccg                                                                                                                                >  4:418786/1‑151 (MQ=11)

         catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAGAGGCAATCATGGTTTGGCCCCGCCAAAAACAAAAACccggcgc                                                                                                                               >  2:1304190/1‑148 (MQ=11)

          atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGAGTCTTGTCAAGTCAATCAAAATTTAACACCAACAAAAACAAAAACCCGCAGAAG‑c                                                                                                                           >  4:1344658/1‑151 (MQ=11)

          atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGTAAAGTCAATCATGATTATGCGCCGACAAAAGCAAAAACCCGCAGAAgg                                                                                                                            >  1:693085/1‑150 (MQ=11)

            cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGTCCATCATGTTTGGTCGGCAGCAAAAACAAAAACCCGCCGAAG‑C‑g‑g                                                                                                                       >  7:1740388/1‑151 (MQ=11)

              ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAGCAGGTGAAACTGACCGATAAGCCGCGTTCTTTGGGGTAGAGGGTCTGGGACAGGCCTGCATTCTTCAGCCGCGGCAATACCAAAAAACCGCCGCAG‑C‑g‑gcg                                                                                                                     >  7:1669321/1‑149 (MQ=2)

               tCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGTATAGCGTCGTGGCGAGGCCATCATGGGTGTGCGCGGGCAAAAGCAAAAACCCGCCGCAG‑g‑g‑gggg                                                                                                                    >  8:496825/1‑149 (MQ=11)

                    cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTAGTGGAAAGTCATTCAAAATTGAGCCCCAACAAAAACCAAAACCCGCCGCAgggcggttata                                                                                                                   >  4:1604715/1‑140 (MQ=11)

                      cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGTGCCCCGGCAAAAGCAAAAACCCGCCGCAG‑C‑G‑GGGTTTTACgg                                                                                                             >  7:262606/1‑150 (MQ=255)

                        ccAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATGTTTGGGCCCCTGCAAAAGCAAAAACCCGCCGCAG‑G‑G‑GGGGTTTTCGTat                                                                                                           >  3:1576284/1‑150 (MQ=11)

                          aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCAAAATTAAGCCCCAGCAAAAACAAAAAACCGCAGCAG‑A‑C‑GGTTTTTAACTaaa                                                                                                          >  4:220718/1‑150 (MQ=11)

                          aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGCGAGGCATTCAAGGTGGGGCGCCGGCAAAAGCCAAAACCCCCCGCAG‑C‑G‑GGGTTTTACggatat                                                                                                         >  8:1654165/1‑146 (MQ=11)

                           aaatcttaaaCCCGCCGTATCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATc                                                                                                        <  6:1401626/144‑1 (MQ=11)

                            cactggacagccgccgACTCCGGTTACTACCTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCa                                                                                                       <  7:794692/141‑1 (MQ=11)

                            aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCAGTCTGCACCTCCAAAAGCAAAAACCCGCCGCAG‑C‑G‑GGTTTTTACGTTAATc                                                                                                        >  6:126985/1‑150 (MQ=255)

                             aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCATCTTTATGCCCCACCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGGTTTTAAGTAAATc                                                                                                        >  1:186736/1‑149 (MQ=255)

                                aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTAGAGGACAGTAATTCATAATTATGAACAATCAAAAACAAAAACCCGCCGAAG‑C‑C‑CGGTTTTAAGGAAATCAGCTg                                                                                                   >  1:809170/1‑151 (MQ=11)

                                 aaaaCCCGCCGCAGCGGGTTATTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGCGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGt                                                                                                    <  7:1409126/149‑1 (MQ=255)

                                  aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTCGACAGGCAATCATCTTTATGCCCCACCAAAACCAAAAACCCGCCGCAC‑C‑C‑GGTGTTTTCCGAAATCAGgaga                                                                                                  >  6:395608/1‑150 (MQ=11)

                                    aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCACGCAATCAAGTTTGTGCCCCGCCAAAACCAAAAAAACCCCCCAG‑CGG‑GGTTTTTAC‑TAAATAACGTGaaa                                                                                                >  2:108462/1‑150 (MQ=2)

                                     cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTTGGTAGAGCGTCGTGGAGACGCCATCATCGTTGTCCCCCGCCAAAAACAAAAAACCGCCGAAG‑C‑GCGGTTTTTCCGaaatgacgtccaac                                                                                                >  2:370775/1‑139 (MQ=2)

                                        cccgcatagttttattACGTAAAATAGTTTAAAATGACAGATTAGCCTCTTTCTTTTTTGTATAGTGTCGTGGAAAGTCATTCATCTTTCTGCCACTCAAAAATCAAAAACCCGCCGAAT‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGAc                                                                                           <  1:85308/137‑1 (MQ=255)

                                         ccttccgagcttattACGTACACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTTTGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGAcc                                                                                          <  2:589977/138‑1 (MQ=11)

                                           gcatcGGGTTTTTACGTACATAATTTTAAAATTACCGATTAGACGATTTCTTTTTTGTATATAGTCTTGTACATTTATTCATCTTTCTGCCTTTCGAAAAGCATAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGa                                                                                        <  6:263918/147‑1 (MQ=2)

                                            acgggtgTTTTTACGTAAAGGATGTTAATCTGAGCTATATTCTGCTTTCTTTTTTCTATAGTGTCGTCGAATGGGTTTTCTATTTCTGCCACTCAAAAATCTAAAACCCGCCGAAT‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGAt                                                                                       <  2:78313/145‑1 (MQ=255)

                                             accGGGTTATTACGTACATAATTTTAAACTCAACGATAAGCCGCTTTCTTTTGTGTATAGCGTAGTGGATAGTCATTCATGTATCTGCTCCTATAAAATCAAAAACCCGCCGAAT‑C‑G‑GGTTTTTACGTAAATCAGGTTAAACTGACCGATa                                                                                      <  4:346989/149‑1 (MQ=255)

                                              gCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTAAAATTTATACACCACCAAAAACAAAAACCCGCCTAAG‑A‑C‑GGTTTTTAAGTAAAACAGGGGAAAATTACCCATa                                                                                      >  4:1183746/1‑150 (MQ=11)

                                                 ggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAACATTGGGCGCCGCCAAAAACAAAAACCCGCCGAAG‑C‑C‑GGGTTTTAAGTAAATCACCGGAAACttcccccaacgcc                                                                                  >  8:669433/1‑139 (MQ=11)

                                                  gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCAATCAAAATTATGCGCCGGCAAAAACAAAAACCCGCAGCAG‑A‑G‑CGGTGTTAAGGAAATGAGGGGAAAGTTGCCCTTAAGCCg                                                                                 >  5:848884/1‑151 (MQ=2)

                                                  gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGGGATTAATGTTTTTGCCCCCACAAAAACAAAAAACCGCCGCAC‑G‑G‑GGGTTTTTCCTAAATGACGTGAAAATGACCGATAAGccc                                                                                 >  4:1028288/1‑150 (MQ=2)

                                                     tttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCATGTTTGTGCCCCTCCAAAAGCAAAAACCCGCCGCAG‑A‑G‑GGTTTTTACGTAAATCAGGTGAAAATGACCGATAATCCGGGt                                                                              >  3:940496/1‑151 (MQ=14)

                                                      ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCCATCAAAGTTAGGCCCCAGCAAAAACAAAAACCCGCCGCAG‑A‑C‑GGGGTTTAAGTAAATCAAGGGAAAATTCCCCATAAGCCGGGtt                                                                             >  1:1532505/1‑151 (MQ=9)

                                                      ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATCATAATTGAACCCCAACAAAAACAAAAAACCCCCGAAC‑A‑G‑CGTTTTTACATAAATAAGCTCAAAATTACCGATAAGCCGGGtt                                                                             >  5:1792011/1‑151 (MQ=9)

                                                       tACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCCTTGAACTTTGGGGCCCGCCAAAATCAAAAAACCCCCGCAG‑G‑G‑GGGTTTTTCGTTAATCAGGTGAAAATGGccccttccccgggttg                                                                            >  2:990068/1‑137 (MQ=2)

                                                        aCGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCAATAAACATTGAGACCCGGCAAAAACAAAAAAACCCCGCAG‑C‑C‑GGTTTTTAACTAAAAAAGCTGCAAAATCCCGATAACCCGGGGTCt                                                                           >  2:45638/1‑151 (MQ=2)

                                                          gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAGAGGCATTCATATTTCGGCCCCAACAAAAGCAAAAACCCGCCGCAG‑C‑G‑GGTTTTTAAGTAAATCACGGGAAACTGACCGATAAGCCGGGTTCTGt                                                                         >  8:338223/1‑151 (MQ=14)

                                                           tAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGCAAGACATTCATCGTTCTGCGCCACCAAAAGCAAAAACCCCCGGCAGAC‑G‑GGGATTAACG‑AAATCAAGAGCAACTTACCCATAAGCCGGGATCTGt                                                                         >  5:366204/1‑150 (MQ=9)

                                                             aaacagattaccctcaCCGATATTCCTCTTTCTTTTGCTTATATCTTCGTGTACACGAATTCATTTTGATGTCCCTCCAAAAGTAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                                                      <  4:1812164/136‑1 (MQ=9)

                                                             aaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCAAGATTATGCCCCGGCCAAACCAAAAACCCGCCGAAG‑A‑C‑GGGTATTAAGAAAATCACGGGAAAATTACCCATAAGCCGGGTTCTGTAGt                                                                      >  4:1285596/1‑151 (MQ=9)

                                                                cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTGATGGTTGTGCCCCTCCAAAAGCAAAAACCCCCCGCAG‑C‑G‑GGGTGTTTCGTTAATCACGTGCAACTGACCGCTAATCCGGGTTCTGTCGTggg                                                                   >  3:536577/1‑150 (MQ=14)

                                                                 aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTGCATGATATTTTTGGCCCGCCAAAAACAAAAACCCGCCGCAG‑A‑G‑GGGTTTTTAGTTAATAAGGGGAAAATTACCGATAATCCGGGTTGTGtggtgggg                                                                  >  4:414951/1‑149 (MQ=9)

                                                                   gggAAACTGACCGATCAGCCGCGTTCTTTTGGGTTTTTTTTTTTTTTCAGTGATTAATCTTTCTGCCTATACAATAGCAAAAACTCTTTGAAT‑A‑G‑GGGTTTTTCTTAATTTATGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAg                                                                <  6:905364/149‑1 (MQ=255)

                                                                      aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAG‑C‑G‑GGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtc                                                              >  8:87542/1‑150 (MQ=255)

                                                                      aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTGTGCCCCAGCAAAACCAAAAAACCCCCGCAG‑C‑C‑GGGTTTTACGTAAATCAGGTGAAACTGACCCAAAAGCCGGGTTCTGtagggggccgtc                                                              >  4:1001785/1‑139 (MQ=14)

                                                                       acgctcttcctatctcGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtca                                                             <  1:1081514/135‑1 (MQ=14)

                                                                       aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTGACCCCCCAAAAAAACAAAAACCCGCCCAAG‑C‑G‑GGTTTTTAAGTAAATAAGCTGAAAATGACCGAGAAGCCCGGTTATGTCGTGGaaacgaa                                                             >  2:43526/1‑144 (MQ=14)

                                                                        aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCCCCAAAACCAAAAACCCGCCGCAG‑G‑C‑GGGTTTTAAGTAAATCAGGTGCAACTGCCCCATAAGCCGGGTTCTGTAGTGGGCCGtcatt                                                           >  7:890208/1‑151 (MQ=14)

                                                                         cTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTCATTCATATTTATTCCCCTACAAAAACAAAAACCAGCCGAAG‑C‑G‑GGGTTTTAAGTAAATCAGGTGAAACTGACCGAAAATCCGGGTTCTGTAGTGGCAAGtcatt                                                           >  8:1210754/1‑150 (MQ=14)

                                                                         cTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCAAAATTATGCCCCTACAAAAGCAAAAAACCGCCCAAG‑C‑C‑CGTTTTTAAGTAAATCAAGTTAAAATGAAAGATAAAACGGGTTATGTAGTGGACAGtcagt                                                           >  5:1595493/1‑148 (MQ=9)

                                                                          tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATTCGCCTCCAAAACAAAAAAACCGACGAAC‑C‑G‑GGGTTTTTCGTAAATCAGGTTAAACTGACCGAAAACCCGGGTTCTGTTGTGGCCAGtgatta                                                          >  3:1157595/1‑149 (MQ=14)

                                                                          tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATAATAATTATCCCCCAACAAAAGCAAAAACCCCCCGAAA‑C‑G‑GCGTTTTAAGTAAATCACGTGAAACTGACCCAAAAGCCGGGTTGTGTCGGGGACAGtcattc                                                          >  6:515759/1‑150 (MQ=17)

                                                                            aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGtcattaat                                                        >  8:1180059/1‑150 (MQ=255)

                                                                              cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTAAGTAAATCACGTGAAACAGACCGAAAAGCCGGGTTCTGTCGTGGACAGTCAATAATCt                                                      >  3:1317055/1‑150 (MQ=17)

                                                                               gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATATTTATGCCCCTCCAAAAGCAAAAAACCGCCGAAC‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTGTGTGGTGGACAGTCATTGATCTat                                                    >  2:1168039/1‑150 (MQ=17)

                                                                                aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGGTTTTACGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCTAgg                                                   >  7:804786/1‑151 (MQ=255)

                                                                                 tAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATCTTTCTGCCCCAACAAAAGCAAAAACCCGCCGAAG‑A‑C‑GGTTTTTAAGTAAACCAGGTGAAACTGAACGATAAGCCGGGTTCTGTTGGGGAAAgagaattagcaaggc                                                  >  6:1110184/1‑137 (MQ=11)

                                                                                 tAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGc                                                  >  5:771261/1‑151 (MQ=255)

                                                                                   aGCCGCTTTCTTTTGGGTATAGCGTCGTGGACACTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACCTAAATCAGGTGAAACAGACCCATAAGCCGGGTTATGTCGTGGACAGTCATTAATCTAGGcc                                                 >  8:793889/1‑150 (MQ=17)

                                                                                     ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTATTCCCCAACAAAAGCAAAAAACCCCCGAAG‑C‑G‑GGTTTTTAAGTAAATAAGGTGAAACTGACCGATAACCCGGGTTCTGTACTGGACACTCAATCATCTATGCCatc                                              >  5:444645/1‑149 (MQ=17)

                                                                                      cGCTTTCTTTTTGGTTTTCCGTCGTGGACTGTCATGCTTTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCa                                             <  4:655494/151‑1 (MQ=18)

                                                                                      cGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCCGc                                              >  3:1288631/1‑150 (MQ=255)

                                                                                      cGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGGCAGTCATTCATCTAGGCCAGc                                              >  5:676031/1‑150 (MQ=255)

                                                                                      attttttttttttttataTCGTCGTCGACAGTCATTTATATTTTTTCCTATACACTAGCAAAACACCTCTGTAG‑T‑G‑TGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCa                                             <  4:550565/137‑1 (MQ=16)

                                                                                       gCTTTCTTTTGGGTATAGCGTCGTGGGCAGTCATTAATATTTATGCCCCTCCAAAAGCAAAAAACCCCCGAAC‑C‑G‑GGGTTTTACGGAAAACAGGGGAAACTGACCCATAAGCCCGGTTCTGTCGTGGGCACTGATTAATCTACGCCaccc                                             >  2:1372050/1‑147 (MQ=255)

                                                                                       gCTTTCTGTAGGCTCTTGGGTCGTGGAAAGCGATTCCTCTTGATGCCCCTCCACAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCaa                                            <  3:566779/151‑1 (MQ=18)

                                                                                         tttctttTGGGTATAGCGTCGTGGGCAGTCATTGATCTTTGTGGCCCTCCAAAAGCAAAAAACCCCCGAAG‑C‑G‑GGTTTTTACGTAAATAAGGTGAAACAGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCTAGGCCAGCAAt                                           >  7:946058/1‑150 (MQ=255)

                                                                                          ttctttTGGGTATAGCGTCGTGGGCAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCCCCGAGG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGGCAGTCATTCATCTAGGCCAGCAATCg                                         >  7:465690/1‑151 (MQ=255)

                                                                                            ctttTGGGTATAGCGTCGTGGACAGTAATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTAAGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTGATTCATCTAGGCCAGCAATCGcg                                       >  6:765686/1‑150 (MQ=255)

                                                                                             ttttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCAGCCCCGCCAAAAACAAAAACCCGCCCCAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGGTCTGTCCTGGCCAGTCATTCATCTAGGCCAGCaaaagct                                       >  4:1444302/1‑145 (MQ=17)

                                                                                                 gggTATAGTTTCGTGTACATTTATTTTTATTTATGCCACTCAAAAATCATAAACCCGCCGTAT‑C‑T‑GTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTg                                  <  1:982576/151‑1 (MQ=21)

                                                                                                    tataGCGTCGTGGCCAGGCATTCATCTTTCTGCCCCTCCAAAACCAAAAAACCCCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCCTTCATCTAGGCCAGCCATCGCTCACTGGcg                               >  8:586266/1‑150 (MQ=21)

                                                                                                     ataGCGTCGTGGACAGTCATTCATATTTCTGCCCCTACAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGcgc                              >  5:1688661/1‑149 (MQ=255)

                                                                                                      taGCGTCGTGGCCAGTCATTCATCTTTCTGCCCCTCAAAAAGAAAAAACCCGCCGAAG‑G‑G‑GGTTTTTACGTAAATAAGGGGAAACTGCCCGAAAACCCGGGTTATGGCATGGACAGTCATTAAAATAGGCAAGAAATCGCGCACTGGcga                              >  8:1035322/1‑148 (MQ=17)

                                                                                                       cccctcggGGACAGGCATTCATCTTTCAGCCCCTCCAAAAGGAAACACGCGGAGACG‑A‑T‑TGTTTTTACGTAAATCAGTTTAAACTGACCGATAAGGCGGTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCaa                            <  7:37761/147‑1 (MQ=21)

                                                                                                       aGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCa                             <  4:708490/150‑1 (MQ=255)

                                                                                                           tcggatataGTCATTCATCTTTCTGCAACTCCAAAAGTAAAATCGCGTAGAAG‑A‑T‑TGTTTTTACTTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagca                         <  1:1010125/142‑1 (MQ=21)

                                                                                                             gggttctgggtttcttcTTTATGGCACTCCAAAAGCATAAACCCGCCGTAT‑C‑G‑GGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc                      <  4:809994/140‑1 (MQ=25)

                                                                                                               ggACAGTCATTCATCTTTCTGCCACTCCAAAAGTAAAAACCCGCCGAAG‑C‑G‑TGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt                     <  5:1188007/150‑1 (MQ=255)

                                                                                                                gACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAc                   >  8:1495996/1‑151 (MQ=255)

                                                                                                                gACAGGCATTCATGTTTATTCACCTCCAATAGCAAAAACGAGCTTCAT‑G‑G‑AGTTTTTAGGTAAAGCCGCTTCATCTGTTCGCTTATTCGGTTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAc                   <  7:1168788/151‑1 (MQ=255)

                                                                                                                 atattgattcttCTTTCTGCCACTTCTAAAGCAAATACTAGACGAAG‑T‑G‑GGTTTTTAAGTATATCAGGTGATTCTGATTGATATTGGGGTTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAc                   <  5:1154/144‑1 (MQ=255)

                                                                                                                 aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAcc                  >  5:307664/1‑151 (MQ=255)

                                                                                                                   aGTCCTTCATCCTTCTGCCCACCCCACAGCACACCCGCGCCGAAG‑C‑G‑CGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCg                <  2:609470/151‑1 (MQ=25)

                                                                                                                   aGTCATTCATCTTTCTGCCCCTCCAACACCAAACACGCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGGCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCg                <  3:775967/151‑1 (MQ=255)

                                                                                                                   aGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTGGTGTATAGTCACTAGTCTAGGCAATCAATCGGTAACTCGCTCAagcaagatagca                 >  7:1011783/1‑142 (MQ=11)

                                                                                                                        ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTAGTGGACAGTCTGTGGTCTAGTCCAGCAAACTTTCACTGCCTCAaccacaaaaacccggcgc           >  8:914825/1‑137 (MQ=2)

                                                                                                                         tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc           >  3:254145/1‑150 (MQ=255)

                                                                                                                              tttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTAGTGGAGAGTCTGTGATCTAGGc                                                  >  7:685040/1‑106 (MQ=17)

                                                                                                                               ttCTGCCCCTCCAAACGGAAAACCCGGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTAc      <  7:1622300/149‑1 (MQ=255)

                                                                                                                               ttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc           <  6:1060821/144‑1 (MQ=255)

                                                                                                                               ttCTGCCCCTCCAAAAGCAAAACCCCGCGGAAG‑C‑T‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGt        <  7:1810919/147‑1 (MQ=255)

                                                                                                                               ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑T‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTAc      <  1:1425160/149‑1 (MQ=255)

                                                                                                                               ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTa       <  3:1717635/148‑1 (MQ=255)

                                                                                                                               ttCTGCACCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACg     <  1:312896/150‑1 (MQ=255)

                                                                                                                                tCTGCTCCTACATAAGTAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACgg    <  1:1369754/150‑1 (MQ=255)

                                                                                                                                tCTGCCCCTCCAAAAGCAAAAACCCTCCTAAG‑T‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACgg    <  6:811650/150‑1 (MQ=255)

                                                                                                                                tCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCTATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACgg    <  2:1665302/150‑1 (MQ=255)

                                                                                                                                 cTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGGCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGc  >  6:1300739/1‑151 (MQ=255)

ACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑C‑G‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGC  >  NC_000913/3270038‑3270317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`