Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT24.0% 882.5 / inf 630intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (225/212);  new base T (56/82);  total (325/305)
Fisher's exact test for biased strand distribution p-value = 3.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-18

Only 100 of 638 total aligned reads displayed.

AGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAC  >  NC_000913/4295910‑4296210
                                                                                                                                                      |                                                                                                                                                      
     ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCTTCGACGccgg                                                                                                                                                   >  8:1694928/1‑149 (MQ=9)
     ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTg                                                                                                                                                   >  2:570123/1‑151 (MQ=14)
     ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAAGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg                                                                                                                                                   >  3:1784509/1‑151 (MQ=14)
      gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTACTCCGCATCCGACATCAATGACTg                                                                                                                                                   >  5:1522599/1‑150 (MQ=9)
      gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTg                                                                                                                                                   >  1:1773630/1‑150 (MQ=14)
        tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATg                                                                                                                                                >  5:717866/1‑151 (MQ=14)
         acggCGTTTATGCCGCATCAGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc                                                                                                                                               <  6:28738/149‑1 (MQ=14)
         aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                                                                               >  1:163841/1‑151 (MQ=14)
         aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc                                                                                                                                               <  7:469282/151‑1 (MQ=31)
          aGGCGTTTATGCCGCCCCCGACAGCAACGCCTCATGCGACGCATCACGCGTCTTATCAGTCCTACGCCAGACCGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                                                                              <  7:1621679/151‑1 (MQ=11)
          aGGCGTTTATGCCGCATCCGACATCAAAGCCTGAAGCGACGATTAACGCCTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGAGTGATTTTGTAGGTCGGATAAGGCGTTTCCGCCGCATCCGCCCTCCCCGCCTGCTGCg                                                                                                                                              >  3:939956/1‑151 (MQ=11)
            gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACCCCGCATACGACATCAAAGCCTGATGCGAc                                                                                                                                            >  8:1169261/1‑151 (MQ=9)
              gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACgg                                                                                                                                          >  4:350529/1‑150 (MQ=14)
              gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAGGCGACgg                                                                                                                                          >  2:1466967/1‑150 (MQ=14)
               tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                                                                         >  3:526237/1‑151 (MQ=31)
                ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                                                                         <  4:150208/150‑1 (MQ=31)
                 tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGAGTGATTTTGTAGGTCCGATAAGGCGTTTACGACGCATCAGACATCAATGCCTGATGCGACGCTTg                                                                                                                                       >  5:1605090/1‑151 (MQ=11)
                  aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc                                                                                                                                      <  2:89800/151‑1 (MQ=33)
                  aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc                                                                                                                                      <  6:565924/151‑1 (MQ=33)
                   tGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTACGCCGCAGCCGACAACACCCCCTCATGCGACGCttcac                                                                                                                                     >  3:106673/1‑148 (MQ=11)
                    gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                                                                               <  7:113663/140‑1 (MQ=17)
                     ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGGCAGAAAGCGCAATAGCCTGATGTAGCGTCATTTTGTAGGTCGGATAAGGCGTTTACGCCGCCTCCGACATCAACGCCTGATGCGACGCTTGCcgc                                                                                                                                   >  2:802436/1‑151 (MQ=255)
                      ggCATGCGAGATCAACGCGTGAGTCGAGCCGTCCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgcg                                                                                                                                  <  7:1400450/150‑1 (MQ=11)
                      cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgcg                                                                                                                                  >  3:1689803/1‑151 (MQ=21)
                        cacccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGTCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCTGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgcg                                                                                                                                  <  5:407849/146‑1 (MQ=255)
                        cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                                                                                >  5:1076975/1‑151 (MQ=21)
                        cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                                                                                >  7:950907/1‑151 (MQ=21)
                           ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                             <  6:810454/151‑1 (MQ=35)
                            cGACATCACCGCCTGAGGCGACGCGTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                            <  2:1751825/151‑1 (MQ=255)
                            cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                            >  4:1048053/1‑151 (MQ=21)
                            cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                            <  2:1691625/151‑1 (MQ=35)
                             gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                           >  1:1191991/1‑151 (MQ=21)
                               cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                          <  5:1358071/150‑1 (MQ=35)
                               cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                         <  2:207358/151‑1 (MQ=35)
                               aaTCAACGCCTGATGCGACGCTTAACGTGTCTTATCATGCCTACGCCAGACAGGGTAATAGGATGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                         <  6:986471/150‑1 (MQ=11)
                                aTCAACGCCTGTTGCGACGCTTAACGCGTTTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                                        <  1:1443157/151‑1 (MQ=19)
                                aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGCTCGGATAAGGCGATTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                                        >  4:957370/1‑151 (MQ=25)
                                  cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                      >  5:1716181/1‑151 (MQ=21)
                                  cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                      >  7:670848/1‑151 (MQ=21)
                                  cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                      <  3:1405009/151‑1 (MQ=35)
                                   acggCCTCAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                                                     <  4:1343266/148‑1 (MQ=255)
                                   aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                                                     >  2:744640/1‑151 (MQ=21)
                                     ggCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAGAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                                                                                   <  6:1572608/150‑1 (MQ=25)
                                     cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAAGCAAGACAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCTGATAAGGCGTTTACGCCGCATCCTACATCAAAGACTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                                                                                   >  6:735855/1‑151 (MQ=11)
                                      gCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACTCTTGCCGCGTCTTATCAGGCCTACg                                                                                                                  <  3:461371/151‑1 (MQ=25)
                                       ccTGCTGCGCCGCTTACCGCGTCTTTTGTGGGGTTCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATCAGGCGTTTACGCCGGATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                                                                                 <  1:135498/151‑4 (MQ=11)
                                       ccTGATGAGACGCGTCCCGCGCCTAATCCGGCCGACGCCAGAGAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                                                                                 <  7:332659/151‑4 (MQ=11)
                                        cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                                                                                >  2:1613698/1‑147 (MQ=14)
                                        cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTAGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                                                                                >  4:117422/1‑147 (MQ=9)
                                         tGATGCGACGCTTTACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGcc                                                                                                                >  4:1628338/1‑150 (MQ=14)
                                         tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACggcc                                                                                                               >  2:1250715/1‑148 (MQ=25)
                                         cgaggCGACGCTTCCCGCGGCTTATCAGGCCTACGCCAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGGCGGATACGGCGTTTACGCCGCATCCGACAGCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta                                                                                                               <  8:1562639/147‑6 (MQ=11)
                                          gATGCGACTCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCTCAATAGGCTGATTTATCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                                                                                <  1:715020/149‑5 (MQ=11)
                                          gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta                                                                                                               <  8:728972/150‑6 (MQ=9)
                                          gATCCGACGCTTAAATCGTATTATCAGGCCTACTCCAGACATCTCAATTGCCTGATTTATCGTGATTTTGTAGGTCGGATAAGTCGTTTTCGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta                                                                                                               <  4:801028/150‑6 (MQ=11)
                                             gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAGAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa                                                                                                           <  4:487339/151‑1 (MQ=255)
                                              cGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAGGCCGGAcgg                                                                                                          >  2:1345645/1‑149 (MQ=255)
                                               gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTagcgcagcccgg                                                                                                         >  2:1054262/1‑140 (MQ=255)
                                                aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCGCGCCTACGCCAGCCcgcg                                                                                                        >  3:724362/1‑151 (MQ=255)
                                                  gCTTACCGCGTCTTAGCAGGCCTACGCCAGACAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCa                                                                                                      <  8:352907/151‑1 (MQ=255)
                                                  gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGCTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGACTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc                                                                                                       >  2:459981/1‑150 (MQ=25)
                                                   cTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCGAGCCCGCGCat                                                                                                     >  7:399249/1‑150 (MQ=255)
                                                      aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAGGCGAgccgggggatt                                                                                                    >  3:1317407/1‑139 (MQ=11)
                                                      aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGACAGACAGCGCAAt                                                                                                    >  4:691342/1‑149 (MQ=25)
                                                            tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTGGTATAAGGCTTTTACTCCGCATCAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCt                                                                                               <  6:1408668/148‑1 (MQ=255)
                                                            gcgcagcacgCCTACGCCACACAGCGCAATACCCTGATTTAGCGTGATTTTGTTTGTCGCATAAGCAGTTTACTCAGCATCCTACATCACCTGCTTATGCGACGCTTTCCGCTTCTTATAACTCCTACGCCAGACAGCGCAATAGCCTGAt                                                                                            <  7:757244/142‑1 (MQ=2)
                                                                        tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGCCTGCAAAGCGGGATCCGAGGCAGTCCCCTGCTAATAAGCGATAGCCGAGACAGCGCAATCGACTGATATAGAGTGAttt                                                                                >  2:114134/1‑151 (MQ=0)
                                                                          cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCTTATCAGGAGAACGCAAGACAGACCAATAGCCTAATATAGAATGATATTg                                                                              >  6:1123619/1‑151 (MQ=11)
                                                                             cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAAGCCAGACAGCGCAATAGCCTGAATTAGCTTGAGTTTGt                                                                             >  6:673427/1‑149 (MQ=255)
                                                                                    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAACAAAAACAACAAAATAACCTCATGTAGAGTGATAATGTAGTTCGCAt                                                                    >  1:438081/1‑151 (MQ=11)
                                                                                       aaTAGCTTGATTTAGCGTTATTTTGTTGGGCTCATACTCGGTTTACGGAGCATCCTATTTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg                                                                 <  4:603689/151‑1 (MQ=11)
                                                                                          aGCATGATTTAGCGTGATTTTGTAGCTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCg                                                              <  8:284922/151‑1 (MQ=25)
                                                                                            ccTGATTTAGGGTGATTTTTTAGGGCTTATCAGGCTTTTACTCCGCCTACGTCTTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtt                                                            <  7:1192712/151‑1 (MQ=11)
                                                                                            aatGATTTAGCGTGATTTTTTAGGGAGTAGACGGCCTTTACGCCGCATAAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtt                                                            <  8:475681/149‑1 (MQ=11)
                                                                                              tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCAAGACAGAGCAATAGGATGATTTATCGTGATTTTCTAGGTCGGATAAGTCGttt                                                           >  5:93819/1‑150 (MQ=11)
                                                                                               gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAGAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt                                                         >  7:1815777/1‑151 (MQ=25)
                                                                                                  ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCAAGCCAGCGCAATAGGCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc                                                      >  8:1336965/1‑151 (MQ=255)
                                                                                                     gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAAGAAAGACAGAGCAAAATAATGATTTACCGTGAATATGTATGTAGGATAAGGCGTTTATgaagc                                                    >  6:1655505/1‑146 (MQ=11)
                                                                                                              tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATc                                          >  7:1661332/1‑151 (MQ=35)
                                                                                                              tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGAAGCATAAGGAGTTTATgaagaatgagacat                                           >  1:266455/1‑137 (MQ=11)
                                                                                                               ttttttattatatGGCTTTTACGCCGCTTAAGACTTCAAGGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATc                                          <  1:752854/141‑1 (MQ=11)
                                                                                                                   ggcggagcagccGTTTACGCCGCAGCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGc                                     <  2:351130/140‑1 (MQ=11)
                                                                                                                   gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGc                                     >  8:1798726/1‑151 (MQ=35)
                                                                                                                     cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAACCAAGACAGCGCAATAGACTGATTTAGCGTGATTTTCTATGTCGGATAAGGCGTTTAAGCCGCATACGACATCAACGACt                                   >  6:457824/1‑151 (MQ=11)
                                                                                                                      ggATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTg                                  >  3:1182771/1‑151 (MQ=35)
                                                                                                                       gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTg                                  >  8:596099/1‑150 (MQ=35)
                                                                                                                        cgacggCGTATACCCCGCCTAAGACATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAt                                <  3:1544525/147‑1 (MQ=11)
                                                                                                                          aaGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGc                              >  4:200859/1‑151 (MQ=35)
                                                                                                                           cggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCg                             <  8:864242/150‑1 (MQ=25)
                                                                                                                             gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAc                           >  5:539791/1‑151 (MQ=25)
                                                                                                                              cggatACGCCGCATCCGACATCAACCCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACg                          <  3:368005/147‑1 (MQ=25)
                                                                                                                                 ttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCt                        >  5:1799734/1‑150 (MQ=35)
                                                                                                                                    cgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAc                    >  8:1024394/1‑151 (MQ=21)
                                                                                                                                      ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCTCGTCTTATAAGGCCTACGCCAGACAGGTGAATAGCCTGATTTAGTCTGATTTTTTAGTTCTGATATGGCTTTTTTGCTGCATCCGACATCAACGCCTGATGCGACGCTTAAcgc                  <  1:540874/151‑1 (MQ=11)
                                                                                                                                        ccagccGACACCCACCCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCATAAAGAGCAATAGCCTGATTTAGCGTGATTTTGTATTTCGTATAAGGCTTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt                <  1:932066/147‑1 (MQ=11)
                                                                                                                                         cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc               >  5:1173872/1‑151 (MQ=33)
                                                                                                                                         cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAAGCCTGATGCGACGCTTAACGCGTc               >  7:135135/1‑151 (MQ=18)
                                                                                                                                            ccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAATGCGATTATGCCGCATCCGACATAAACGCCTGATGAGACGCTTAAAGCGTCtt             >  1:1015808/1‑150 (MQ=255)
                                                                                                                                             cGACATCAAAGCCTGCTGCTACGGTTGCGGCTTCTTAGCAGGCCTACTCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTAt           <  3:782521/151‑1 (MQ=11)
                                                                                                                                                 aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCGGGCCTACGCAAGACAGCGCAAAAGCCTGATTTAGAGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACATCAAAGACTGATGCGACGCTTTCCGCGTCTTATCAgg       >  1:1560175/1‑151 (MQ=11)
                                                                                                                                                      |                                                                                                                                                      
AGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAC  >  NC_000913/4295910‑4296210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: