Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4290TG30.9% 691.9 / 122.2 413intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (69/213);  new base G (121/5);  total (195/218)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 437 total aligned reads displayed.

GGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422279‑422579
                                                                                                                                                      |                                                                                                                                                      
   ttCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgcct                                                                                                                                                     >  3:809034/1‑151 (MQ=255)
    tCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcct                                                                                                                                                     <  7:1113208/150‑1 (MQ=255)
      gcgGGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcct                                                                                                                                                  <  1:1180954/151‑1 (MQ=255)
       cgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcctc                                                                                                                                                 <  1:710848/151‑1 (MQ=255)
        gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGgcctgctcc                                                                                                                                                >  5:609516/1‑151 (MQ=255)
         tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCGCCGgccgccggcc                                                                                                                                               >  3:353891/1‑147 (MQ=255)
          ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGTCCTcccc                                                                                                                                              >  6:340624/1‑151 (MQ=255)
          ggATTGTTGTGCTGTTGATTCGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTcccc                                                                                                                                              <  5:1493620/151‑1 (MQ=255)
              tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCTCCCCCaa                                                                                                                                           >  7:1311966/1‑150 (MQ=255)
                  gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATGCGCGCCCGACCTCCGGCCCCAAaaat                                                                                                                                       >  4:655333/1‑150 (MQ=255)
                   tgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACAAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAACTTATCCGCCCCCCGCCTAGACCCCCAAATATcc                                                                                                                                     >  5:715559/1‑151 (MQ=255)
                       gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTc                                                                                                                                  <  3:1743648/150‑1 (MQ=255)
                         ggagtGGCTGGATGTCTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAga                                                                                                                               <  1:57264/147‑1 (MQ=255)
                          gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                              <  1:1411748/151‑1 (MQ=255)
                          gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                              <  2:1633904/151‑1 (MQ=255)
                          gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAga                                                                                                                               <  6:452636/150‑1 (MQ=255)
                            ttGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAATATCCTTCAgatg                                                                                                                             >  6:1359847/1‑150 (MQ=255)
                                cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCCAAAATCCGTCAGatggtgcg                                                                                                                        >  3:266513/1‑149 (MQ=255)
                                      gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATcc                                                                                                                  <  6:1759542/151‑1 (MQ=255)
                                       tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCTCCTCCCCCAAATTAGGTAGAGATGATGAGTGATGCt                                                                                                                 >  4:521855/1‑151 (MQ=255)
                                         tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                                <  8:116449/150‑1 (MQ=255)
                                          aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGGAGTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGTAATCATGATCAGGCTATTCCGAAAGTTATCAGACCACGTAATCCGCCGCGCACTGTCGTTCAGATGGTGAGTgggaggggg                                                                                                              >  1:792322/1‑143 (MQ=255)
                                            aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                            <  4:789260/151‑1 (MQ=255)
                                             cgccgcCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAAAATCCTTCAGATGATGAGTGGTCCTGCagtt                                                                                                           >  5:443512/1‑148 (MQ=255)
                                                cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCCAATATACTTCAGATGATGAGTGATCCTGCATTAggg                                                                                                        >  4:1386573/1‑150 (MQ=255)
                                                     cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATgg                                                                                                   <  8:1007566/151‑1 (MQ=255)
                                                     cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATgg                                                                                                   <  8:74422/151‑1 (MQ=255)
                                                     cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAAAATCCTTCAGATGATGAGTGATCGGGCATTAGGCTATgg                                                                                                   >  4:206363/1‑151 (MQ=255)
                                                      gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTTGGCTATggg                                                                                                  >  6:1641829/1‑150 (MQ=255)
                                                      gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCACCTAGCCTGCAGATGAGGAGTGGTCCTGCATTTGGGGATggg                                                                                                  >  8:825684/1‑150 (MQ=255)
                                                       aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                                 <  3:86869/151‑1 (MQ=255)
                                                        tCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCGCCCCCCAAAAGCCTGCAGATGATGCGTGAGCGTGCAGTGGGGTAGGGc                                                                                                  >  4:41186/1‑149 (MQ=255)
                                                          gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                              <  2:467594/151‑1 (MQ=255)
                                                           tCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGGCCCCAAATATAATTCACATGATGAGTGTTCCTGCATTAGGCTATGGCAAGGt                                                                                             >  4:1511310/1‑151 (MQ=255)
                                                            cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCCAATATCCTTCAGATGAGGCGTGATCCTGCATTAGGCTATGGCAAgggg                                                                                            >  1:321094/1‑149 (MQ=255)
                                                              gctggctgAAAACCAGTACTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt                                                                                          <  5:1680848/151‑1 (MQ=255)
                                                              gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCTCCCCCAAATATCCTTCAGAGGATGAGTGCTCCGGCATTTGGCGATGGCCAGGTg                                                                                            >  8:418970/1‑149 (MQ=255)
                                                              gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCACATATCCTTCAGATGATGCGTGATGCTGGGTTAGGGTATGGCAAGGTggt                                                                                          >  4:1140505/1‑149 (MQ=255)
                                                                 ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAg                                                                                       <  8:31065/151‑1 (MQ=255)
                                                                 ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAg                                                                                       <  7:574935/151‑1 (MQ=255)
                                                                    tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCCCATATACTTGAGATGATGAGTGGGCCTGCATTAGGCTCGGGCAAGGTgcgcagttt                                                                                    >  8:188419/1‑143 (MQ=255)
                                                                      aaaaCCAGTATTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTGCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATAAGAtttt                                                                                  >  4:1060662/1‑151 (MQ=255)
                                                                      aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCAAAGATCCTTCAGAGGATGTGTGGTGCGGGCTTAGGCGATGGCAAGGTgcgccgctttt                                                                                  >  3:1016232/1‑141 (MQ=255)
                                                                      aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtttt                                                                                  <  6:1050451/151‑1 (MQ=255)
                                                                       aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                                 <  3:1594980/151‑1 (MQ=255)
                                                                         aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCGGGCCGCCTCCCCCTCAAATCCTTCAGAGGATGAGGGCTCCTGCATTAGGCTATGGCATGGTGagaatctgttctt                                                                               >  8:1745119/1‑139 (MQ=255)
                                                                         aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                                               <  4:1554605/151‑1 (MQ=255)
                                                                          ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatc                                                                              >  2:570754/1‑151 (MQ=255)
                                                                           cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                                             <  6:743896/151‑1 (MQ=255)
                                                                              caaTCCTGGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg                                                                          <  5:1262949/150‑1 (MQ=255)
                                                                                aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAAAATACTTAAGATGATGAGTGATCCTGCATTAGGCTATTGCAAGGTGATCAGATTTTCATCACAggg                                                                        >  4:609722/1‑151 (MQ=255)
                                                                                  ccTTGCGGTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                       <  7:1176086/150‑1 (MQ=255)
                                                                                     tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGaa                                                                     <  3:610533/149‑1 (MQ=255)
                                                                                     tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                    <  4:341752/150‑1 (MQ=255)
                                                                                       cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                                 <  6:597981/151‑1 (MQ=255)
                                                                                          tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGGGTGATCCTGCATTAGGGTATGGCATGGTGAGCAGAGTTTCATCGCTGGGGAATTatgat                                                              >  2:268520/1‑151 (MQ=255)
                                                                                          tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCGGCATTAGGGGATGGCTAGGTGATGAGAGTTTCATCACAGGGGAATTatgtt                                                              >  5:528314/1‑149 (MQ=255)
                                                                                             gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                           <  5:1093201/151‑1 (MQ=255)
                                                                                             gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                            <  3:1241680/150‑1 (MQ=255)
                                                                                             gTAAGCCGGGTCAGGAGGAAATCCGGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                              <  2:740538/148‑1 (MQ=255)
                                                                                               aaGCCGGTTCAGGATGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTa                                                          <  7:636878/150‑1 (MQ=255)
                                                                                               aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCGTTCAGAAGATGAGTGATGCTGCAGTAGGCGATGGGAAGGTGATCAGATATTCATAACAGGGGAATTATGATGttt                                                          >  1:493835/1‑149 (MQ=255)
                                                                                               aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaa                                                         <  4:1774577/151‑1 (MQ=255)
                                                                                                 gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAAt                                                       <  6:266843/151‑1 (MQ=255)
                                                                                                  ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                      <  3:463492/151‑1 (MQ=255)
                                                                                                     gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                   <  1:90514/151‑1 (MQ=255)
                                                                                                     gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                   <  3:1496969/151‑1 (MQ=255)
                                                                                                      ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCTCCTACCCCAAATAACAATAAGATTATGAGTGATCAAGCATTAGGCTATGTGAACGTGATAAGATTTTAATAAAAAGGGAATTATTATGTTAAAttaatg                                                  >  1:1000563/1‑146 (MQ=255)
                                                                                                        cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGc                                                <  5:850492/151‑1 (MQ=255)
                                                                                                         aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTGCTCCCCCAAATATACTTCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATTGATGGc                                                >  7:438537/1‑150 (MQ=255)
                                                                                                         aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCa                                               <  2:1809159/151‑1 (MQ=255)
                                                                                                             ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTg                                           <  8:582834/151‑1 (MQ=255)
                                                                                                              gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCTCCTCCCCCCAATATCCTTCAGATGATGAGTGATCGGGGATTATGGTATGGCAAGGTGATCAGATTTTGATAACAGGGGAATTATGATGTTAAATGGATGGCACCTGc                                          >  6:807227/1‑151 (MQ=255)
                                                                                                               aaaTCCTGATCCGGCTATTCCGAAACTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                         <  1:1285165/151‑1 (MQ=255)
                                                                                                                   ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTg                                     <  5:943427/151‑1 (MQ=255)
                                                                                                                      gATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATAACCTTCAGATGATGGGTGATCCTGCATTAGGGTATGGCAAGGTGGTGAGATTTTCTTCACAGGGGAATTATTATGTTAAATGAATGGCACCTGCCgggggcc                                  >  7:178760/1‑146 (MQ=255)
                                                                                                                       aTCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTGCAGATGATGAGTGATGCTGCATTAGGCTATGGGAAGGTGGGCAGATTTTCATCACAGGGGAATTATGATGTTTAATGCATGGGACCTGCCGGTGccgc                                 >  8:1357811/1‑149 (MQ=255)
                                                                                                                         ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCa                               <  4:1219385/151‑1 (MQ=255)
                                                                                                                           ggCTAATCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGACTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAtt                             <  1:1825403/151‑1 (MQ=255)
                                                                                                                            gCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAttt                            <  6:1444086/151‑1 (MQ=255)
                                                                                                                             gtATTCCGAAAGCTATCCTCCCACGTCCACCTCCCCCAAAAATCCTTCAGATGATGAGTGAGCCTGCATTAGGCTATCTCAAGGTTATCAGATTTTCATCACAGGGTAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                           <  6:1121340/150‑1 (MQ=255)
                                                                                                                              tATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAAAAACCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATGAGATTTTCATAACAGGGGAATTATGATGTTAAATGAATGTCACCTGCCGGTGGCCCCATTTGt                          >  2:116472/1‑151 (MQ=255)
                                                                                                                              tATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCGGCATTAGGGTATGGGAAGGTGAGGAGATTTTGATCAGAGGGGAATTATGATGTTATATGCATGGGACCTGCCGGTGCGCCCCTTTGt                          >  6:911758/1‑151 (MQ=255)
                                                                                                                               aatCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTTCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGt                          <  1:220923/148‑1 (MQ=255)
                                                                                                                               aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                           <  2:39727/149‑1 (MQ=255)
                                                                                                                               aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                         <  5:1414438/151‑1 (MQ=255)
                                                                                                                                    gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAAAATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATTATTTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                     >  2:598771/1‑150 (MQ=255)
                                                                                                                                      aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaa                  <  7:1684664/151‑1 (MQ=255)
                                                                                                                                       aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag                 <  7:362480/151‑1 (MQ=255)
                                                                                                                                       aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag                 <  7:859958/151‑1 (MQ=255)
                                                                                                                                             ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATTATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag            <  8:470691/150‑1 (MQ=255)
                                                                                                                                               gCCCCCGTCCTCCTCCCCCAATTATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATTGCAAGGTGAGCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACGTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc         <  6:1564263/151‑1 (MQ=255)
                                                                                                                                               gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt          >  8:1462211/1‑150 (MQ=255)
                                                                                                                                               gCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGCTGATCAGATTTTCATCACAGGGGAATTATGAAGTTAAATGCATGGCACCTCACGGTGCCCCCATATGTTAAAAAAACCAAAGAt          >  7:1455588/1‑150 (MQ=255)
                                                                                                                                                  cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc      >  8:1548634/1‑151 (MQ=255)
                                                                                                                                                   ccGTCCTCCTCCCCCAAATATCCTTCAGATGATTAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt     <  1:1711765/151‑1 (MQ=255)
                                                                                                                                                   ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt     <  2:1454539/151‑1 (MQ=255)
                                                                                                                                                    cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg    >  5:260052/1‑151 (MQ=255)
                                                                                                                                                     gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCTTTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc   >  2:1106547/1‑151 (MQ=255)
                                                                                                                                                     gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc   >  1:1588201/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422279‑422579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑