Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,7650G.34.9% 566.1 / inf 233coding (853/1035 nt)tqsApheromone AI‑2 transporter
Reads supporting (aligned to +/- strand):  ref base G (69/82);  new base . (50/31);  total (120/113)
Fisher's exact test for biased strand distribution p-value = 2.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.66e-01

Only 100 of 240 total aligned reads displayed.

TACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCA  >  NC_000913/1674615‑1674912
                                                                                                                                                      |                                                                                                                                                   
tACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATgg                                                                                                                                                     >  6:238014/1‑151 (MQ=255)
tACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATgg                                                                                                                                                     >  8:1464525/1‑151 (MQ=255)
 aCATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATggg                                                                                                                                                    >  3:702868/1‑151 (MQ=255)
 aCATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGGTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATgg                                                                                                                                                     <  3:1138393/150‑1 (MQ=255)
  cATCCCGAATATTGGTTCAGTCCTCGCGGGAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATgggg                                                                                                                                                   <  2:806356/151‑1 (MQ=255)
   aTCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGc                                                                                                                                                  >  3:398932/1‑151 (MQ=255)
   aTCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGc                                                                                                                                                  <  6:567674/151‑1 (MQ=255)
   aTCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGCCTTCTACGAAGCGTTGCTGGTGCTGCCGGGATATCTGCTCATTAATCGGGTCTTCGGCAATATTCTGGCGCCGCGGATCATGGGGc                                                                                                                                                  >  5:415518/1‑151 (MQ=255)
     cccGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGt                                                                                                                                                >  4:433390/1‑151 (MQ=255)
      cagAATATTGGGTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTTGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGcttt                                                                                                                                               <  4:594975/149‑4 (MQ=255)
        gAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGctttcc                                                                                                                                             <  3:535875/151‑6 (MQ=255)
         aaTATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGc                                                                                                                                            >  5:1373903/1‑151 (MQ=255)
         aaTATTGGTGCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGc                                                                                                                                            <  8:1101787/151‑1 (MQ=255)
              tGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGctttccacatt                                                                                                                                        <  3:494630/150‑11 (MQ=255)
               ggTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGctttccccattgg                                                                                                                                      >  7:695825/1‑139 (MQ=255)
                gTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCt                                                                                                                                     >  3:457937/1‑151 (MQ=255)
                  tCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGCGGCGTGGGCTGGGGCttt                                                                                                                                   >  6:758606/1‑151 (MQ=255)
                          cgcgGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtg                                                                                                                           <  1:1099534/151‑1 (MQ=255)
                          cgcgGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtg                                                                                                                           <  2:728033/151‑1 (MQ=255)
                                 aTCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTAt                                                                                                                    >  5:524088/1‑151 (MQ=255)
                                 aTCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGCGATATCTGCTGATTAATCTGCTCTTCGGCAATATTCTGGAGCGGCGTATCATGGCGCGTGGGCAGGGGCTTTCCACATTGGTGGTAt                                                                                                                    >  4:226789/1‑151 (MQ=255)
                                   ccccccTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTAttt                                                                                                                  <  1:1448612/151‑1 (MQ=255)
                                      cccTATCGCTCAGGTACTGGTGTTTAATGTCTTCTACGAAGCGTTGCTGGTGCTGGCGTGATAGCTTCTGATTCATCTGGTCTTCTGAAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGTTGGTATTTTTg                                                                                                               <  2:1248749/151‑1 (MQ=255)
                                          aTCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTggg                                                                                               >  4:1028552/1‑151 (MQ=255)
                                            cGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgggg                                                                                              >  4:1363953/1‑150 (MQ=255)
                                             gCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGa                                                                                                        <  8:64870/151‑1 (MQ=255)
                                              cTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATg                                                                                           >  3:595478/1‑151 (MQ=255)
                                                cAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATg                                                                                           <  4:704904/149‑1 (MQ=255)
                                                 cggTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgtt                                                                                        <  7:736087/150‑1 (MQ=255)
                                                    tACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                     >  7:269667/1‑151 (MQ=255)
                                                    tACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                     >  1:1409572/1‑151 (MQ=255)
                                                       tGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAgg                                                                                  >  2:1471550/1‑151 (MQ=255)
                                                       tGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAgg                                                                                  >  7:1263872/1‑151 (MQ=255)
                                                        ggTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAgg                                                                                  >  2:1162211/1‑150 (MQ=255)
                                                         gtgtTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGAt                                                                                            <  4:1254819/151‑1 (MQ=255)
                                                            tttAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgt                                                                                         >  5:868758/1‑151 (MQ=255)
                                                              tAATGGCTTCTACGAATCGTTGCTGGTGATGGCGGGATATCTCCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGt                                                                            <  6:137778/150‑1 (MQ=255)
                                                               aaTGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTgg                                                                          <  7:1047111/151‑1 (MQ=255)
                                                                aTGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTggg                                                                         <  4:1308562/151‑1 (MQ=255)
                                                                 tGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                     >  6:931395/1‑150 (MQ=255)
                                                                 tGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGCCCGGTGGGt                                                                        >  2:95774/1‑151 (MQ=255)
                                                                  ggCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                     >  1:1392221/1‑149 (MQ=255)
                                                                  ggCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAg                                                                                   <  1:813074/151‑1 (MQ=255)
                                                                     ttCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgg                                                                    >  5:919229/1‑150 (MQ=255)
                                                                     ttCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgc                                                                    >  1:159658/1‑151 (MQ=255)
                                                                      tCTACGTAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGTTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtg                                                                     <  4:905807/149‑1 (MQ=255)
                                                                         aCGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAcc                                                                               <  1:561255/148‑1 (MQ=255)
                                                                         aCGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAcc                                                                               <  2:61227/148‑1 (MQ=255)
                                                                           gAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTgg                                                                          <  3:1451077/151‑1 (MQ=255)
                                                                               ggTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAGCGGTGGGTATGCTGCTTTCCGt                                                          <  4:985251/150‑1 (MQ=255)
                                                                               cGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGt                                                          >  1:659221/1‑151 (MQ=255)
                                                                                 ttGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgc                                                                    >  1:720339/1‑151 (MQ=255)
                                                                                   gctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgctg                                                                  >  4:1099130/1‑151 (MQ=255)
                                                                                   gctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGcc                                                       <  7:1173654/150‑1 (MQ=255)
                                                                                    ctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgctgc                                                                 >  3:165107/1‑151 (MQ=255)
                                                                                       gggctggCGGGATATCTGCTGTTTAATCTGGTCTTCGGCAATTTTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCttt                                                              <  2:27814/149‑1 (MQ=255)
                                                                                          ctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGATGGATTTTTGTCCTTGATTTTTTGGGGATGGTTGTTAGCACCGGTGGGAATGCTGCTTTcct                                                           >  4:817453/1‑150 (MQ=255)
                                                                                           tggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatt                                             >  2:1058142/1‑151 (MQ=255)
                                                                                            ggCGGGATATCTGCTGATTAATCTGGTCTTCGTCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAat                                              <  8:1092539/150‑1 (MQ=255)
                                                                                            ggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGt                                                          <  8:1167254/150‑1 (MQ=255)
                                                                                            ggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatt                                             <  7:517826/151‑1 (MQ=255)
                                                                                             gCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAAGCTGCTTTCCGTGc                                                        <  6:701265/151‑1 (MQ=255)
                                                                                              cGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAattat                                           >  2:570232/1‑151 (MQ=255)
                                                                                               gggATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAattatt                                          >  7:44164/1‑151 (MQ=255)
                                                                                                ggATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTg                                         <  3:521792/151‑1 (MQ=255)
                                                                                                ggATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGGATCATG‑‑‑‑‑‑‑‑‑‑‑GGGGTTTCCACATTGGTGGTTTTTTTTTGGTTGTTTTTTTTGGGATGGTTGTTAGGACCCGGGGGTTTGGTGGTTTACGTGGCGTTTACCattattt                                         >  7:1363557/1‑150 (MQ=255)
                                                                                                 gATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGt                                        <  4:1178193/151‑1 (MQ=255)
                                                                                                 gATATCTGCTGATTAATCTGGGCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGtt                                                    <  6:1454063/151‑1 (MQ=255)
                                                                                                  atatCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTc                                       >  8:325260/1‑151 (MQ=255)
                                                                                                    atCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGa                                                  <  2:665084/150‑1 (MQ=255)
                                                                                                     gctgctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGAGTTTTTGGGGATGGGTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaaa                                    <  1:377710/150‑1 (MQ=255)
                                                                                                        gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACaa                                               <  5:97138/149‑1 (MQ=255)
                                                                                                        gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACa                                                <  5:1083016/148‑1 (MQ=255)
                                                                                                        gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAat                                              <  6:1302309/150‑1 (MQ=255)
                                                                                                        gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaaa                                    <  2:134148/148‑1 (MQ=255)
                                                                                                        gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAt                                  <  8:1263356/150‑1 (MQ=255)
                                                                                                        gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAt                                  <  4:944469/150‑1 (MQ=255)
                                                                                                           gATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATCGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaaaaatccg                              >  6:794769/1‑146 (MQ=255)
                                                                                                            aTTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgcgc                             >  3:953855/1‑151 (MQ=255)
                                                                                                            aTTAAACTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTg                                         >  1:956818/1‑151 (MQ=255)
                                                                                                             ttAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCt                            >  3:1301283/1‑151 (MQ=255)
                                                                                                               aaTCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTc                                       >  5:1313829/1‑150 (MQ=255)
                                                                                                                aTCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCa                                      >  7:1330352/1‑150 (MQ=255)
                                                                                                                 gcTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaaa                                    <  1:853050/150‑1 (MQ=255)
                                                                                                                    ggTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAtt                                 <  3:684619/151‑1 (MQ=255)
                                                                                                                     gTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTg                                >  8:774052/1‑151 (MQ=255)
                                                                                                                          cGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCtt                           <  1:1015276/151‑1 (MQ=255)
                                                                                                                                tatTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATAGTCAAAATTGCGATTGaaca                      >  2:1002903/1‑150 (MQ=255)
                                                                                                                                 atTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaa                    <  7:1467645/151‑1 (MQ=255)
                                                                                                                                 atTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCGTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGtacaaa                    <  3:240838/151‑1 (MQ=255)
                                                                                                                                   tCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaac                   >  8:198571/1‑150 (MQ=255)
                                                                                                                                   tCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaac                   >  1:1190884/1‑150 (MQ=255)
                                                                                                                                      ggAGCCGCGTATGAGGGGGCGTGGGCGGGGGCTTTCCACAGTGGTGGGATTTTTGTCGTTGATTTTTTGGGGATGGGTGTTAGGAGCGGTGGGTATGCTGCTTTCCGTGCCGTTGAGAATTATTGTCAAAATTGCGCTTGaacaaaca                  <  2:992086/148‑1 (MQ=255)
                                                                                                                                           cgcgTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAgg          >  6:1304718/1‑151 (MQ=255)
                                                                                                                                             cgTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTc        <  6:158790/151‑1 (MQ=255)
                                                                                                                                                  cATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGc   <  5:574771/151‑1 (MQ=255)
                                                                                                                                                  cATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGc   <  1:315439/151‑1 (MQ=255)
                                                                                                                                                  cATGGGGCGGGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGGTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGc   <  8:230368/151‑1 (MQ=255)
                                                                                                                                                   aTGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGc   >  4:455800/1‑150 (MQ=255)
                                                                                                                                                   aTGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCa  <  1:283597/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCA  >  NC_000913/1674615‑1674912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: