BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,674,774	0	C	.	37.2%	504.3 / inf	215	coding (862/1035 nt)	tqsA	pheromone AI‑2 transporter
Reads supporting (aligned to +/- strand): ref base C (58/77); new base . (49/31); total (107/108)
Fisher's exact test for biased strand distribution p-value = 1.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-02

Only 100 of 217 total aligned reads displayed.

AATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTG  >  NC_000913/1674624‑1674923

         tCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGCGGCGTGGGCTGGGGCttt                                                                                                                                              >  6:758606/1‑151 (MQ=255)

          cAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTc                                                                                                                                             <  5:1261285/151‑1 (MQ=255)

           aGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTcc                                                                                                                                            >  5:970645/1‑151 (MQ=255)

              ccTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGTGCGTGGGCTGGGGCTTTCcac                                                                                                                                          >  7:828984/1‑150 (MQ=255)

               cTCGCGGCAATCCCCCCTATCGCTCAGGTACTGTTGTTTAATGGCTTCTACGAAGAGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATATGGTCTTAGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACAt                                                                                                                                        >  1:1303106/1‑151 (MQ=255)

                 cgcgGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtg                                                                                                                                      <  1:1099534/151‑1 (MQ=255)

                 cgcgGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtg                                                                                                                                      <  5:770305/151‑1 (MQ=255)

                                 aTCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTggg                                                                                                          >  4:1028552/1‑151 (MQ=255)

                                 aTCGCTCAGGTACTGGTATTTAATGGCTTCTACGAAGAGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGt                                                                                                                      >  2:573664/1‑151 (MQ=255)

                                  tCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgggg                                                                                                         >  1:298554/1‑151 (MQ=255)

                                   cGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgggg                                                                                                         >  4:1363953/1‑150 (MQ=255)

                                    gCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGa                                                                                                                   <  8:64870/151‑1 (MQ=255)

                                    gCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGGCGTTGATTTTTTGGGGAt                                                                                                       >  7:1462008/1‑151 (MQ=255)

                                     cTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATg                                                                                                      >  3:595478/1‑151 (MQ=255)

                                     cTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATg                                                                                                      >  7:242379/1‑151 (MQ=255)

                                       cAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGAtt                                                                                                                 <  4:88845/150‑1 (MQ=255)

                                       cAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATg                                                                                                      <  4:704904/149‑1 (MQ=255)

                                        aGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGAtttt                                                                                                               <  6:572461/151‑1 (MQ=255)

                                         ggTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttg                                                                                                  >  4:910202/1‑151 (MQ=255)

                                           tACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                                >  7:269667/1‑151 (MQ=255)

                                            aCTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgg                                                                                                           <  5:496034/151‑1 (MQ=255)

                                              tGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAgg                                                                                             >  7:1263872/1‑151 (MQ=255)

                                               ggTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAgg                                                                                             <  2:1182432/150‑1 (MQ=255)

                                                      aaTGGCTTCTACGAAGCGTTGCTGGTACTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttg                                                                                                  <  3:108814/150‑1 (MQ=255)

                                                       aTGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTggg                                                                                    <  4:1308562/151‑1 (MQ=255)

                                                        tGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                                >  6:931395/1‑150 (MQ=255)

                                                         ggCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAg                                                                                              <  1:813074/151‑1 (MQ=255)

                                                                aCGAAGCGTTGCTGGTGCTGGCGGGTTATCTGCGTATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATgctgct                                                                           <  2:1379343/151‑1 (MQ=255)

                                                                aCGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAcc                                                                                          <  1:561255/148‑1 (MQ=255)

                                                                 cGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCtt                                                                          >  2:229982/1‑151 (MQ=255)

                                                                  gAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGTGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTgg                                                                                     <  2:620686/151‑1 (MQ=255)

                                                                  gAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTgg                                                                                     <  4:119378/151‑1 (MQ=255)

                                                                  gAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTgg                                                                                     <  5:91134/151‑1 (MQ=255)

                                                                  gAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTgg                                                                                     <  3:1451077/151‑1 (MQ=255)

                                                                  gAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCttt                                                                         >  5:166918/1‑151 (MQ=255)

                                                                    aGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTT‑GGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCg                                                                      >  8:1480168/1‑151 (MQ=255)

                                                                     gCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCAT‑‑‑‑‑‑‑‑‑‑C‑‑GGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTATTGGGGATGGTTGTTAGGACCGGTGGGTATCCTGCTTTCCg                                                                      >  1:712212/1‑151 (MQ=255)

                                                                      ggTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAGCGGTGGGTATGCTGCTTTCCGt                                                                     <  4:985251/150‑1 (MQ=255)

                                                                      cGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTATGACCGGTGGGTAt                                                                                 >  4:959140/1‑151 (MQ=255)

                                                                      cGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGCCCGGTGGGTATGCTGCTTTCCGt                                                                     >  8:1214376/1‑151 (MQ=255)

                                                                       gTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtg                                                                                >  8:11254/1‑151 (MQ=255)

                                                                          gctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGcc                                                                  <  7:1173654/150‑1 (MQ=255)

                                                                          gctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCg                                                                 >  3:1475789/1‑151 (MQ=255)

                                                                           ctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgctgc                                                                            >  3:417898/1‑151 (MQ=255)

                                                                           ctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGt                                                                >  8:1100419/1‑151 (MQ=255)

                                                                           ctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGt                                                                >  1:1398734/1‑151 (MQ=255)

                                                                            tggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTGTCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGtt                                                               >  1:384600/1‑151 (MQ=255)

                                                                            tggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGtt                                                               >  6:1386729/1‑151 (MQ=255)

                                                                             ggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGt                                                                <  3:538512/149‑1 (MQ=255)

                                                                                   ggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatt                                                        <  7:517826/151‑1 (MQ=255)

                                                                                    gCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGc                                                                   >  3:810059/1‑151 (MQ=255)

                                                                                    gCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAAGCTGCTTTCCGTGc                                                                   <  6:701265/151‑1 (MQ=255)

                                                                                     cGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAattat                                                      <  3:238390/151‑1 (MQ=255)

                                                                                      gggATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAattatt                                                     >  7:44164/1‑151 (MQ=255)

                                                                                       ggATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGTTGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAattattt                                                    >  1:1418836/1‑150 (MQ=255)

                                                                                       ggATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTg                                                    <  3:521792/151‑1 (MQ=255)

                                                                                         atatCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTg                                                              <  2:526256/151‑1 (MQ=255)

                                                                                         atatCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTc                                                  >  8:325260/1‑151 (MQ=255)

                                                                                         atatCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTc                                                  >  1:730724/1‑151 (MQ=255)

                                                                                           atCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGa                                                             <  6:994550/150‑1 (MQ=255)

                                                                                           atCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGa                                                             <  2:665084/150‑1 (MQ=255)

                                                                                              tgctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAat                                                         >  7:542066/1‑151 (MQ=255)

                                                                                               gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatt                                                        >  6:1322878/1‑151 (MQ=255)

                                                                                               gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAat                                                         <  2:1336482/150‑1 (MQ=255)

                                                                                               gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAtt                                            <  2:778245/151‑1 (MQ=255)

                                                                                               gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAt                                             <  8:1263356/150‑1 (MQ=255)

                                                                                                  gATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAattatt                                                     >  2:113869/1‑151 (MQ=255)

                                                                                                  gATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAattat                                                      <  6:1151173/150‑1 (MQ=255)

                                                                                                  gATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGTGGATGGTTGTTATGACCCGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTTTCAAAATTgcg                                         >  1:224087/1‑151 (MQ=255)

                                                                                                  gATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTCGGGATGGTTGTTAGGACCGGAGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgcg                                         >  4:407071/1‑151 (MQ=255)

                                                                                                   aTTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgcgc                                        <  5:546089/151‑1 (MQ=255)

                                                                                                   aTTAAACTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTg                                                    >  1:956818/1‑151 (MQ=255)

                                                                                                    ttAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCt                                       >  3:1301283/1‑151 (MQ=255)

                                                                                                      aaTCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTc                                                  >  5:1313829/1‑150 (MQ=255)

                                                                                                       aTCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCa                                                 >  7:1330352/1‑150 (MQ=255)

                                                                                                        gcTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaaa                                               <  1:853050/150‑1 (MQ=255)

                                                                                                         cTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaaa                                               >  6:130245/1‑150 (MQ=255)

                                                                                                           ggTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaaa                                               <  4:1262178/148‑1 (MQ=255)

                                                                                                           ggTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAtt                                            <  3:684619/151‑1 (MQ=255)

                                                                                                           ggTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAtt                                            >  4:795017/1‑151 (MQ=255)

                                                                                                           ggTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAt                                             <  3:458582/150‑1 (MQ=255)

                                                                                                            gTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTg                                           >  8:774052/1‑151 (MQ=255)

                                                                                                            gTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTCGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTg                                           >  6:23092/1‑151 (MQ=255)

                                                                                                                tCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgc                                          <  4:1089993/148‑1 (MQ=255)

                                                                                                                 cGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCtt                                      <  1:1015276/151‑1 (MQ=255)

                                                                                                                       tatTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATAGTCAAAATTGCGATTGaaca                                 >  2:1002903/1‑150 (MQ=255)

                                                                                                                          tCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaac                              >  1:1190884/1‑150 (MQ=255)

                                                                                                                             ggAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaaca                             <  2:833408/148‑1 (MQ=255)

                                                                                                                                   gcgTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGt                    >  1:1456962/1‑151 (MQ=255)

                                                                                                                                   gcgTATCATGGGGCGTGGGCTGGGGCGTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGt                    <  4:1134828/151‑1 (MQ=255)

                                                                                                                                    cgTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTc                   <  6:158790/151‑1 (MQ=255)

                                                                                                                                      tATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTCACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCa                  >  4:220034/1‑150 (MQ=255)

                                                                                                                                         cATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGc              <  1:315439/151‑1 (MQ=255)

                                                                                                                                         cATGGGGCGGGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGGTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGc              <  8:230368/151‑1 (MQ=255)

                                                                                                                                          aTGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTTTTAGGACAGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATAATTGTCAAAATTGCGCTTGAACAAACACAGGGAGGTCAAAGc              >  2:964756/1‑150 (MQ=255)

                                                                                                                                          aTGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGc              >  4:455800/1‑150 (MQ=255)

                                                                                                                                               gCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGcc        <  7:975362/151‑1 (MQ=255)

                                                                                                                                                  tGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGtt     >  6:81229/1‑151 (MQ=255)

                                                                                                                                                   gggCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTc    >  7:1082360/1‑151 (MQ=255)

                                                                                                                                                     gCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCGGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTg  <  3:247092/151‑1 (MQ=255)

AATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTG  >  NC_000913/1674624‑1674923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`