Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,610,1070GA49.1% 16.8 / inf 390E541K (GAG→AAG) hyfGhydrogenase 4, subunit
Reads supporting (aligned to +/- strand):  ref base G (81/117);  new base A (80/111);  total (161/229)
Fisher's exact test for biased strand distribution p-value = 9.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-02

Only 100 of 404 total aligned reads displayed.

AACTGGCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAA  >  NC_000913/2609957‑2610257
                                                                                                                                                      |                                                                                                                                                      
 aCTGGCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAaga                                                                                                                                                       <  5:225538/151‑1 (MQ=255)
    ggCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAagag                                                                                                                                                      <  7:941755/149‑1 (MQ=255)
    ggCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAaaag                                                                                                                                                      <  4:1029853/149‑1 (MQ=255)
     gCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAag                                                                                                                                                        >  8:311774/1‑146 (MQ=255)
     gCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATc                                                                                                                                                   <  2:828105/151‑1 (MQ=255)
        ggTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGACAGTCAAAAACCGTGCCGTATAAAGAGATCGa                                                                                                                                                 >  4:308927/1‑150 (MQ=255)
         gtgtTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAAc                                                                                                                                               <  5:582577/151‑1 (MQ=255)
         gtgtTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAAc                                                                                                                                               <  3:563107/151‑1 (MQ=255)
           gtTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGc                                                                                                                                             <  5:1136957/151‑1 (MQ=255)
           gtTGAGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGc                                                                                                                                             <  1:1107704/151‑1 (MQ=255)
             tGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCt                                                                                                                                            >  6:1404539/1‑150 (MQ=255)
               cGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACg                                                                                                                                         <  6:539277/151‑1 (MQ=255)
                gTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACgg                                                                                                                                        <  4:178777/151‑1 (MQ=255)
                  tACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGc                                                                                                                                       >  4:1304423/1‑150 (MQ=255)
                  tACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGc                                                                                                                                       <  5:1262816/150‑1 (MQ=255)
                   aCATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCAt                                                                                                                                     <  8:1359666/151‑1 (MQ=255)
                      tgctgcGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTg                                                                                                                                   >  1:1209695/1‑150 (MQ=255)
                       gctgcGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGa                                                                                                                                  <  5:1428885/150‑1 (MQ=255)
                        ctgcGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGAt                                                                                                                                 >  6:1444960/1‑150 (MQ=255)
                        ctgcGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATc                                                                                                                                >  1:1345507/1‑151 (MQ=255)
                          gcgcGGCAATACCGTTTCTGACGCAGCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGAGCGTGTGACGGTGGTAGATGTGCGCAAGGGCGAGGCAAAAACCGTGCGGTATATAAAGATCGAACGCTAGGGCATTGATCg                                                                                                                               <  4:252838/150‑1 (MQ=255)
                           cgcgGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTTCTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTa                                                                                                                             <  2:1032490/151‑1 (MQ=255)
                           cgcgGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGt                                                                                                                              >  7:467493/1‑150 (MQ=255)
                              ggCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTGCGGCATTGATCGTAAcc                                                                                                                          <  5:692596/151‑1 (MQ=255)
                                    aCCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTcg                                                                                                                     <  2:590998/150‑1 (MQ=255)
                                    aCCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAAGCGTTcgc                                                                                                                    <  8:748035/151‑1 (MQ=255)
                                    aCCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCACTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGGTcgc                                                                                                                    >  3:1281119/1‑151 (MQ=255)
                                       gTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTcgccgc                                                                                                                 <  4:833623/151‑1 (MQ=255)
                                         ttCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCt                                                                                                                <  1:822859/150‑1 (MQ=255)
                                              aCGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTa                                                                                                          <  1:972647/151‑1 (MQ=255)
                                               cGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCAGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTaa                                                                                                         <  5:393036/151‑1 (MQ=255)
                                                 cACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAgg                                                                                                       <  5:896461/151‑1 (MQ=255)
                                                 cACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAgg                                                                                                       <  5:826412/151‑1 (MQ=255)
                                                 cACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTAGCCGCTGAAGTAAgg                                                                                                       >  7:1371847/1‑151 (MQ=255)
                                                     gCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACag                                                                                                   <  8:660842/151‑1 (MQ=255)
                                                       tGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACaga                                                                                                  >  7:737592/1‑150 (MQ=255)
                                                        gATTATCGTTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACagaa                                                                                                 <  4:113208/150‑1 (MQ=255)
                                                        gATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACagaag                                                                                                <  4:757296/151‑1 (MQ=255)
                                                        gATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACaga                                                                                                  <  8:806359/149‑1 (MQ=255)
                                                        gATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACaga                                                                                                  >  3:1105128/1‑149 (MQ=255)
                                                         aTTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACagaaga                                                                                               <  2:174859/151‑1 (MQ=255)
                                                         aTTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGAGCCGTATAAAGAGATCGCACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACaga                                                                                                  >  3:161397/1‑148 (MQ=255)
                                                              cGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTg                                                                                          >  1:913849/1‑151 (MQ=255)
                                                                  aGCCTTGATCCCTGCTACTCCTGTACCGACCGTGGGACGCTGGTAGATGTGCGCAAGCGGCAGTCAAAAACCGTGCCGTATATATAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGt                                                                                      <  8:439955/151‑1 (MQ=255)
                                                                    ccTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTa                                                                                    <  1:647819/151‑1 (MQ=255)
                                                                    ccTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAATTAAGGACAGAAGATGCTGAAGTTa                                                                                    <  8:1158037/151‑1 (MQ=255)
                                                                    ccTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTa                                                                                    <  1:312611/151‑1 (MQ=255)
                                                                       tGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTAc                                                                                   <  6:473847/149‑1 (MQ=255)
                                                                       tGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACt                                                                                  <  1:190321/150‑1 (MQ=255)
                                                                        gATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGa                                                                                <  5:161059/151‑1 (MQ=255)
                                                                           cccTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGaaaa                                                                             <  2:362339/151‑1 (MQ=255)
                                                                           cccTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGaaa                                                                              <  4:683191/150‑1 (MQ=255)
                                                                           cccTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGaaa                                                                              >  3:963207/1‑150 (MQ=255)
                                                                             cTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACt                                                                           >  5:1387360/1‑151 (MQ=255)
                                                                             cTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACAGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCAGAAGTTACTGAAAACt                                                                           >  7:1489360/1‑151 (MQ=255)
                                                                               gCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACt                                                                           <  4:465324/149‑1 (MQ=255)
                                                                                cTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACtatt                                                                        >  7:1311324/1‑151 (MQ=255)
                                                                                  aCTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACtatta                                                                       <  7:554764/150‑1 (MQ=255)
                                                                                  aCTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACtattat                                                                      <  2:1115333/151‑1 (MQ=255)
                                                                                  aCTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACtatta                                                                       >  2:1392580/1‑150 (MQ=255)
                                                                                  aCTCCTGTAACGACCGTGTGACGCTGGTAGATGTGAGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACtattat                                                                      <  8:594832/151‑1 (MQ=255)
                                                                                     ccTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATgc                                                                    <  6:919577/150‑1 (MQ=255)
                                                                                        gTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATgcgc                                                                  <  3:1388376/149‑1 (MQ=255)
                                                                                          accgaccgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCg                                                              <  7:796810/151‑1 (MQ=255)
                                                                                           ccgaccgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCgg                                                             <  6:334718/151‑1 (MQ=255)
                                                                                              accgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAAc                                                          <  8:268540/151‑1 (MQ=255)
                                                                                              accgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAAc                                                          <  5:111456/151‑1 (MQ=255)
                                                                                              accgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAAc                                                          <  7:269037/151‑1 (MQ=255)
                                                                                               ccgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAAcc                                                         <  5:654163/151‑1 (MQ=255)
                                                                                               ccgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAAcc                                                         <  2:939316/151‑1 (MQ=255)
                                                                                                cgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACcg                                                        <  3:838003/151‑1 (MQ=255)
                                                                                                    tgACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGAc                                                    >  2:731640/1‑151 (MQ=255)
                                                                                                      aCGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACgg                                                  >  5:356518/1‑151 (MQ=255)
                                                                                                          tGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGa                                               >  5:763236/1‑150 (MQ=255)
                                                                                                           ggTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGaa                                              >  5:1030890/1‑150 (MQ=255)
                                                                                                            gTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAat                                            >  1:720983/1‑151 (MQ=255)
                                                                                                             tAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAat                                            >  4:894736/1‑150 (MQ=255)
                                                                                                              aGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGACCGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAatat                                          <  6:1202537/151‑1 (MQ=255)
                                                                                                                   tgCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGACCCGCGACGGTGAAATATCCCtt                                     >  6:1323357/1‑151 (MQ=255)
                                                                                                                    gcgcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCtt                                     <  7:1136928/150‑1 (MQ=255)
                                                                                                                      gcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGACCCGCGACGGTGAAATATCCCTTcgc                                  >  2:461803/1‑151 (MQ=255)
                                                                                                                      gcAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTcgc                                  >  2:251223/1‑151 (MQ=255)
                                                                                                                       cAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTcgcg                                 >  4:534762/1‑151 (MQ=255)
                                                                                                                        aaGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCgcgc                                >  4:1452890/1‑151 (MQ=255)
                                                                                                                         aGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGcc                               <  1:796874/151‑1 (MQ=255)
                                                                                                                           cgcCAGTCAAATACCGTGCCGTATAAAAAGATCGAACGCTACGGGATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAAGTATTATGCGCTCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCAc                             <  8:162262/151‑1 (MQ=255)
                                                                                                                               aGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTgg                          >  3:762147/1‑150 (MQ=255)
                                                                                                                               aGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTgg                          >  4:1180548/1‑150 (MQ=255)
                                                                                                                                   aaaaaCCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTc                     >  7:590136/1‑151 (MQ=255)
                                                                                                                                    aaaaCCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCa                    <  7:66356/151‑1 (MQ=255)
                                                                                                                                       aCCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGcc                 >  4:1090341/1‑151 (MQ=255)
                                                                                                                                        ccGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGccc                >  1:1022187/1‑151 (MQ=255)
                                                                                                                                         cGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCt               <  5:508816/151‑1 (MQ=255)
                                                                                                                                           tGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTgg             >  6:185928/1‑151 (MQ=255)
                                                                                                                                            gCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGc            >  7:1037304/1‑151 (MQ=255)
                                                                                                                                            gCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGc            <  6:701657/151‑1 (MQ=255)
                                                                                                                                              cGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCtt          >  6:637725/1‑151 (MQ=255)
                                                                                                                                               gTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCttt         >  1:1469467/1‑151 (MQ=255)
                                                                                                                                                  taAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTcgc      >  1:448618/1‑151 (MQ=255)
                                                                                                                                                   aaaaAGAACGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTcgcg     <  1:396575/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AACTGGCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAA  >  NC_000913/2609957‑2610257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑