Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913363,4000A.42.6% 706.0 / inf 325coding (2906/3075 nt)lacZbeta‑D‑galactosidase
Reads supporting (aligned to +/- strand):  ref base A (81/105);  new base . (52/86);  total (133/192)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 329 total aligned reads displayed.

AACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACAATGGCAGATCCC  >  NC_000913/363250‑363550
                                                                                                                                                      |                                                                                                                                                      
    ggTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGt                                                                                                                                                   <  6:33784/151‑1 (MQ=255)
      tAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGtt                                                                                                                                                  >  1:1002177/1‑151 (MQ=255)
          ggTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATTCTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGt                                                                                                                                             <  6:702263/151‑1 (MQ=255)
             aGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTg                                                                                                                                           >  3:496672/1‑151 (MQ=255)
             aGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTg                                                                                                                                           <  1:346555/150‑1 (MQ=255)
                gACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACt                                                                                                                                        >  3:1155061/1‑151 (MQ=255)
                 aCCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTg                                                                                                                                       >  2:55751/1‑151 (MQ=255)
                  cgggCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGt                                                                                                                                      <  5:404842/149‑1 (MQ=255)
                     gcgcTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGc                                                                                                                                   <  8:342709/151‑1 (MQ=255)
                     gcgcTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCg                                                                                                                                  <  1:524636/151‑1 (MQ=255)
                      ggcTGAGCTGGAATTCCGCCGATTCTGACGTGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCg                                                                                                                                  <  4:198564/150‑1 (MQ=255)
                      cgcTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCg                                                                                                                                  <  6:1252521/151‑1 (MQ=255)
                          cAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCt                                                                                                                               <  3:410142/150‑1 (MQ=255)
                           aGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTg                                                                                                                              >  4:902703/1‑150 (MQ=255)
                            gCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATg                                                                                                                           >  1:1271858/1‑151 (MQ=255)
                              tGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGt                                                                                                                          >  7:324419/1‑151 (MQ=255)
                              tGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGt                                                                                                                          >  1:1234348/1‑151 (MQ=255)
                                gAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGa                                                                                                                       <  4:1190373/151‑1 (MQ=255)
                                gAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGa                                                                                                                       <  7:859115/151‑1 (MQ=255)
                                 atttgggcggATACTGACTGTCTCCAGGAGTCGTCGGCACCATTCCCCATTTGGATACCGTCGATATTCAGCCTTGTGCCTTCTTCCGCGTGCAGCAGATGGGGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCTGCTGATGTTGa                                                                                                                       <  1:162170/141‑1 (MQ=255)
                                   ttCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCGTGCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAAc                                                                                                                     >  7:245190/1‑151 (MQ=255)
                                     ccgccgATACTGACGGGCTCCAGGAGTCGGCGCCACCAATCCCCATATGGAAACCGGCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTgg                                                                                                                  <  2:271983/151‑1 (MQ=255)
                                       gccgATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGaa                                                                                                                <  1:969166/151‑1 (MQ=255)
                                          gATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGt                                                                                                              >  2:939377/1‑151 (MQ=255)
                                           aTACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTcg                                                                                                            <  3:1124150/151‑1 (MQ=255)
                                           aTACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTcg                                                                                                            <  6:412814/151‑1 (MQ=255)
                                                 aCGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCcgcg                                                                                                       >  4:1201924/1‑151 (MQ=255)
                                                       tCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTg                                                                                                 >  2:1343242/1‑151 (MQ=255)
                                                        ccAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTg                                                                                                 <  5:1207679/149‑1 (MQ=255)
                                                         cAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGgt                                                                                               <  4:726091/151‑1 (MQ=255)
                                                         cAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGgtg                                                                                              <  5:12592/151‑1 (MQ=255)
                                                         cAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGgtg                                                                                              <  3:967016/151‑1 (MQ=255)
                                                             aGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATCGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTgg                                                                                           >  8:923974/1‑151 (MQ=255)
                                                              gtcgtcGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTggg                                                                                          >  8:714338/1‑151 (MQ=255)
                                                                   cgCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATaa                                                                                    <  8:268249/151‑1 (MQ=255)
                                                                     ccaccaATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATaat                                                                                   <  6:1049984/151‑1 (MQ=255)
                                                                     ccaccaATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATaat                                                                                   >  7:1265584/1‑151 (MQ=255)
                                                                      caccaATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATaattc                                                                                 <  3:1092418/151‑1 (MQ=255)
                                                                       accaATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATaattc                                                                                 <  5:595328/151‑1 (MQ=255)
                                                                       accaATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATaattc                                                                                 <  6:913997/151‑1 (MQ=255)
                                                                       accaATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATaattc                                                                                 <  7:1228458/151‑1 (MQ=255)
                                                                            tCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATaattcaattc                                                                            <  2:725511/150‑1 (MQ=255)
                                                                             ccccATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTcgc                                                                          <  1:847231/151‑1 (MQ=255)
                                                                               ccATATGGAAACCGTCGATATGCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGGGGGCCATAATTCAATTcgcg                                                                         <  6:784794/151‑1 (MQ=255)
                                                                                   atGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACAGGTGTGGGCCATAATTCAATTCGCTCGTc                                                                     >  6:1109584/1‑151 (MQ=255)
                                                                                   atGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTcc                                                                    >  6:489762/1‑151 (MQ=255)
                                                                                   atGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCAGAATTCAATTCGCGCGTcc                                                                    >  2:1481147/1‑151 (MQ=255)
                                                                                     ggAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTccc                                                                   >  1:742696/1‑151 (MQ=255)
                                                                                     ggAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCcg                                                                  >  7:361245/1‑151 (MQ=255)
                                                                                     ggAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCcg                                                                  <  8:1143193/151‑1 (MQ=255)
                                                                                      gAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCcg                                                                  <  6:617868/151‑1 (MQ=255)
                                                                                       ataCCGTCGATATTCAGCCATGTGCCTTCTTCCGCGGGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGATCTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTGAATTCGCGCGTCCcgc                                                                 <  5:1023149/149‑1 (MQ=255)
                                                                                       aaaCCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCcgc                                                                 >  3:856586/1‑151 (MQ=255)
                                                                                        aaCCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCcgc                                                                 >  5:521651/1‑149 (MQ=255)
                                                                                             tCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCcgcagcgc                                                            >  4:1409349/1‑149 (MQ=255)
                                                                                              cGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGa                                                         <  2:508109/151‑1 (MQ=255)
                                                                                               tatatTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCcgcagcgc                                                            >  3:641229/2‑148 (MQ=255)
                                                                                               gATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGAc                                                        <  6:1014568/151‑1 (MQ=255)
                                                                                                atatTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGAc                                                        >  8:448942/1‑151 (MQ=255)
                                                                                                  atTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGATTCAATCAGTTGCTGTTGACTTTAGAGGCTGATTTTGAACTGGAATTCGACGAGCCACTCGTCTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCg                                                      >  4:77577/1‑151 (MQ=255)
                                                                                                     cAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGttt                                                   <  5:771944/151‑1 (MQ=255)
                                                                                                      aGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGtttt                                                  >  1:1448097/1‑151 (MQ=255)
                                                                                                         cATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCt                                              >  1:1207156/1‑151 (MQ=255)
                                                                                                          aTGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTc                                             <  2:1192654/151‑1 (MQ=255)
                                                                                                          aTGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTc                                             <  7:1146944/151‑1 (MQ=255)
                                                                                                           tgtgCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCg                                            >  2:654621/1‑151 (MQ=255)
                                                                                                            gtgCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTc                                             <  5:899548/150‑1 (MQ=255)
                                                                                                            gtgCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCg                                            <  6:1211862/150‑1 (MQ=255)
                                                                                                                cttcttCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGa                                         >  3:699590/1‑150 (MQ=255)
                                                                                                                 ttcttcCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGa                                         >  3:181404/1‑149 (MQ=255)
                                                                                                                  tcttcCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGaa                                        <  5:1457586/149‑1 (MQ=255)
                                                                                                                     tcCGCGTGCAGGAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGAc                                     <  4:937388/148‑1 (MQ=255)
                                                                                                                      ccGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTa                                  <  5:1297844/151‑1 (MQ=255)
                                                                                                                      ccGCGTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTa                                  <  7:567051/150‑1 (MQ=255)
                                                                                                                       cgcgTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGTTGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTAc                                 <  4:293191/151‑1 (MQ=255)
                                                                                                                       cgcgTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTAc                                 >  7:480366/1‑151 (MQ=255)
                                                                                                                       cgcgTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACg                                >  5:29264/1‑151 (MQ=255)
                                                                                                                       cgcgTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACg                                >  8:1439206/1‑151 (MQ=255)
                                                                                                                       cgcgTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACg                                <  2:617250/151‑1 (MQ=255)
                                                                                                                          gTGCAGCAGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACgggg                             >  1:27264/1‑151 (MQ=255)
                                                                                                                           ggcagcaGATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTCTCGCTCGGGAAGACGTACGGGGt                            <  4:1326753/150‑1 (MQ=255)
                                                                                                                              agcagATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGtata                         <  8:616763/151‑1 (MQ=255)
                                                                                                                              agcagATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGtat                          >  5:1085518/1‑150 (MQ=255)
                                                                                                                               gcagATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATAc                        >  8:769195/1‑151 (MQ=255)
                                                                                                                               gcagATGGCGATGGCTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATAc                        >  8:1023951/1‑151 (MQ=255)
                                                                                                                                cagATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATAc                        <  1:170246/151‑1 (MQ=255)
                                                                                                                                cagATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATAc                        >  7:1460116/1‑151 (MQ=255)
                                                                                                                                  gatggggatgggTGGTTTGC‑TGTGTTGCTGTTGACTGTAGCGGCTGATGTTGATCTGGAAGTCGCCGCGGCACTGGTGTGGGCGATAATTGAATTCGCGCGTCCCGCAGCGCAGACGGTTTTCGCTCGGGAAGACGTACGGGGTATACATg                     <  6:1293630/151‑1 (MQ=255)
                                                                                                                                       cgatggcTGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTg                 >  3:65534/1‑150 (MQ=255)
                                                                                                                                           ggcTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACa              >  3:884128/1‑150 (MQ=255)
                                                                                                                                           ggcGGGTTTCC‑TCAGTTGCTGGTGACTGTAGCGGCTGATGTTGAACTGGAAGGCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGGCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACAAt            <  6:280761/151‑1 (MQ=255)
                                                                                                                                             cTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACAATg           >  1:866742/1‑151 (MQ=255)
                                                                                                                                              tGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACAATgg          >  4:165951/1‑151 (MQ=255)
                                                                                                                                              tGGTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACAATGGc         >  3:1299808/1‑151 (MQ=255)
                                                                                                                                               ggTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACAATGGc         <  8:1268441/150‑1 (MQ=255)
                                                                                                                                                gTTTCC‑TCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACAATGGCAg       <  7:275233/151‑1 (MQ=255)
                                                                                                                                                   tCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACAATGGCAGa      <  4:650842/150‑1 (MQ=255)
                                                                                                                                                     cATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGCCAATGGCAGATcc   >  2:831171/1‑151 (MQ=255)
                                                                                                                                                      aTCATTTTCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACAATGGCAGATccc  <  7:657900/151‑1 (MQ=255)
                                                                                                                                                      aTCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACAATGGCAGATccc  <  4:425249/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACAATGGCAGATCCC  >  NC_000913/363250‑363550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑