| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,039,960 | Δ1 bp | intergenic (+42/‑36) | yihD → / → yihE | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,039,959 | 0 | A | . | 99.2% | 539.8 / ‑3.1 | 121 | intergenic (+41/‑37) | yihD/yihE | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (61/58); minor base C (1/0); total (63/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
CGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATAATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTAC > NC_000913/4039884‑4040029 | cgcTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATa > 1:4690182/1‑76 (MQ=255)cgcTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATa > 1:226612/1‑76 (MQ=255) gcTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATat > 2:5706668/1‑75 (MQ=255) tGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATatcc < 1:1607056/76‑4 (MQ=255) ttACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCtt < 2:5807381/76‑1 (MQ=255) ttACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCtt < 2:488557/76‑1 (MQ=255) ttACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCtt < 1:965471/76‑1 (MQ=255) tACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTc < 1:188648/76‑1 (MQ=255) aCGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCg < 1:3880066/76‑1 (MQ=255) gcgcTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTc > 2:1274896/1‑76 (MQ=255) cTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCAtt > 1:3186978/1‑76 (MQ=255) cTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCAtt > 1:4220892/1‑76 (MQ=255) cTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCAtt < 1:2320630/76‑1 (MQ=255) cTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCAtt < 2:3267190/76‑1 (MQ=255) tCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTc < 2:3496606/76‑1 (MQ=255) tCGCGGCCTACTTAAAGAGCAGAAACTAGGATGCGGCGACACCAAAATCATCGTGAAATGAT‑ATCCCTCGTCagta > 2:1950134/1‑73 (MQ=255) gcggcgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGt < 2:3355494/76‑1 (MQ=255) gcggcgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGt > 1:5343063/1‑76 (MQ=255) gcggcgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGt > 2:5639703/1‑76 (MQ=255) cggcgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTa < 2:5195516/76‑1 (MQ=255) ggcgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTaa > 2:1819760/1‑76 (MQ=255) gcgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAAt < 1:5816875/76‑1 (MQ=255) gcgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAAt > 1:1208710/1‑76 (MQ=255) cgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATg > 1:4519411/1‑76 (MQ=255) gTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGt < 2:3205321/76‑1 (MQ=255) gTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGt > 1:710574/1‑76 (MQ=255) gTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGt > 1:4760904/1‑76 (MQ=255) gTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGt < 1:4559532/76‑1 (MQ=255) tAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGtt < 1:3613557/76‑1 (MQ=255) tAATTAAAGAGTAAAAGCTGGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGtt < 1:5760679/76‑1 (MQ=255) aaTTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGttt > 1:2975439/1‑76 (MQ=255) aaTTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGttt > 1:1430458/1‑76 (MQ=255) aaTTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGttt < 1:320853/76‑1 (MQ=255) aTTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGtttt > 2:921096/1‑76 (MQ=255) aTTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGtttt < 1:3773735/76‑1 (MQ=255) tgaaaGAGTAAAAGCGTGGAAGCGGAGACACCAAAAGCGTAGTGGAATGA‑GATCAGTCGGCATTCGTAATGTTGTc > 1:1869028/3‑76 (MQ=255) tAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTcc < 2:862243/76‑1 (MQ=255) aaGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCgg > 1:1694245/1‑76 (MQ=255) aaGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCgg > 2:2300926/1‑76 (MQ=255) aaGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCgg < 1:522719/76‑1 (MQ=255) agagTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGga < 2:3626358/76‑1 (MQ=255) gagTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGgat > 1:2793961/1‑76 (MQ=255) gagTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGgat < 1:4779884/76‑1 (MQ=255) agTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGgatg < 2:317727/76‑1 (MQ=255) agTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGgatg > 1:5137349/1‑76 (MQ=255) agTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGgatg < 2:5282721/76‑1 (MQ=255) gTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGgatga < 2:4246766/76‑1 (MQ=255) gTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGgatga > 1:946730/1‑76 (MQ=255) aaaaGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGatgatg > 2:4553097/1‑76 (MQ=255) aaaaGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGatgatg > 2:3543664/1‑76 (MQ=255) aaGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATggg > 1:1213502/1‑76 (MQ=255) aGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGa < 1:921096/76‑1 (MQ=255) gCTTGTGAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGAt < 1:3436540/76‑1 (MQ=255) cTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATg < 1:1476775/76‑1 (MQ=255) ttGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGa > 1:1875224/1‑76 (MQ=255) ttGTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCGTCGTCATTCGTAATGTTTTCCGGATGATGGGATGa > 1:3063305/1‑76 (MQ=255) gTAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAAt < 2:2108360/76‑1 (MQ=255) taggcggggCCACCAAAATCAGCGGGAAATGAT‑ATCCTTCGTCATTCGTAATGTGTTCCGGATGAGGGGATGAATa < 2:3861407/73‑1 (MQ=255) tAAGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATa < 2:3849176/76‑1 (MQ=255) aaGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATaa > 1:3219409/1‑76 (MQ=255) aaGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATaa > 1:5477773/1‑76 (MQ=255) aaGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATaa > 2:454115/1‑76 (MQ=255) aaGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATaa < 2:68258/76‑1 (MQ=255) aGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAAc > 2:5611618/1‑76 (MQ=255) aGCGGCGCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAAc > 2:598052/1‑76 (MQ=255) gcggcgCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACa < 2:5023402/76‑1 (MQ=255) ggcgCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAgc < 2:5357158/76‑1 (MQ=255) ggcgCCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAgc > 1:4387055/1‑76 (MQ=255) gcgcGACCAAAAGCATCTTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAgcg < 2:2819882/76‑1 (MQ=255) gcgcCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAgcg > 2:4662580/1‑76 (MQ=255) cgcCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAgcgc < 2:1868969/76‑1 (MQ=255) cgcCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAgcgc > 2:3126868/1‑76 (MQ=255) gcCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCt > 2:4663925/1‑76 (MQ=255) gcCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCt > 1:1013157/1‑76 (MQ=255) gcCACCAAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCt > 2:5404530/1‑76 (MQ=255) ccaccaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCtt > 1:4752309/1‑76 (MQ=255) ccaccaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCtt > 2:385438/1‑76 (MQ=255) caccaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCttt < 1:1740959/76‑1 (MQ=255) accaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCtttt < 2:3529710/76‑1 (MQ=255) accaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCtttt > 2:4255905/1‑76 (MQ=255) accaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCtttt < 1:2633360/76‑1 (MQ=255) ccaAAATCATCGTGAAATTAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGCATGAGTAACATCGCTTTTa < 1:2531181/76‑1 (MQ=255) ccaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTa > 1:4230896/1‑76 (MQ=255) ccaAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTa < 2:1065984/76‑1 (MQ=255) caAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTAc > 1:1591820/1‑76 (MQ=255) caAAATCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTAc > 2:289782/1‑76 (MQ=255) aaaaTCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACt > 1:2689453/1‑76 (MQ=255) aaaTCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACtt > 2:1782798/1‑76 (MQ=255) aaTCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACttt > 1:4309599/1‑76 (MQ=255) aaTCATCGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACttt < 1:2310130/76‑1 (MQ=255) atcatcGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTc > 1:482073/1‑76 (MQ=255) tcatcGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTcc > 1:1335471/1‑76 (MQ=255) catcGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCa < 1:1465725/76‑1 (MQ=255) catcGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCa > 1:3933789/1‑76 (MQ=255) atcGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAg > 1:2802366/1‑76 (MQ=255) atcGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAg > 2:3383152/1‑76 (MQ=255) tcGTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGa > 1:1222366/1‑76 (MQ=255) gTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGaca > 2:398849/1‑76 (MQ=255) gTGAAATGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGaca > 2:4296857/1‑76 (MQ=255) aaTGAT‑ATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTac < 1:2506224/76‑1 (MQ=255) | CGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATAATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTAC > NC_000913/4039884‑4040029 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |