Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,051,144 Δ1 bp intergenic (+146/+305) hemN → / ← glnG O2‑independent coproporphyrinogen III oxidase/GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,051,1430C.100.0% 346.0 / NA 110intergenic (+145/+306)hemN/glnGO2‑independent coproporphyrinogen III oxidase/GlnG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (52/58);  total (52/58)

Only 100 of 117 total aligned reads displayed.

TGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCA  >  NC_000913/4051069‑4051213
                                                                          |                                                                       
tGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGca                                                                        <  1:2591284/76‑2 (MQ=255)
tGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGca                                                                        >  2:4306138/1‑75 (MQ=255)
tGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGca                                                                        >  1:4843782/1‑75 (MQ=255)
 gATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGcat                                                                       <  1:134234/76‑3 (MQ=255)
 gATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTATTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGcat                                                                       >  1:3140997/1‑74 (MQ=255)
  aTGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGcatc                                                                      >  2:2910344/1‑73 (MQ=255)
     cgcTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGa                                                                  <  1:1470501/76‑1 (MQ=255)
     cgcTGGGCAACAAAACCTGGTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGa                                                                  <  2:5467166/76‑1 (MQ=25)
      gcTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAg                                                                 <  2:2736941/76‑1 (MQ=255)
      gcTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAg                                                                 <  2:3464226/76‑1 (MQ=255)
       cTGGGCAACAAAACCTGTTGAAATTCTTGCTTTTGTATACCGGATAAGGCGTTTACGCCGCATCAGG‑CATCCGAGc                                                                >  2:1277260/1‑76 (MQ=21)
       cTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGc                                                                >  1:2310128/1‑76 (MQ=255)
        tGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCa                                                               >  2:2035207/1‑76 (MQ=255)
        tGGGCAACAAAACCTGTTGAAATTATTACTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCa                                                               >  2:4636613/1‑76 (MQ=25)
           gCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatga                                                            <  2:710266/76‑1 (MQ=255)
           gCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatga                                                            >  2:2397993/1‑76 (MQ=255)
            caacaaAACCTGTTGAACTTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatgat                                                           <  2:1330575/76‑1 (MQ=25)
            caacaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatgat                                                           <  1:4374143/76‑1 (MQ=255)
            caacaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatgac                                                           >  2:3471424/1‑75 (MQ=255)
             aacaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatgatg                                                          >  1:5526191/1‑76 (MQ=255)
             aacaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatgatg                                                          <  2:2026204/76‑1 (MQ=255)
              acaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGc                                                         <  2:2107749/76‑1 (MQ=255)
               caaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGcc                                                        <  2:4843781/76‑1 (MQ=255)
                aaaaCCTGTTGAAATTATTGCGTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCt                                                       <  2:4722932/76‑1 (MQ=25)
                 aacccTGTTGAACTTATTGCTCTTGTAGGCCGGATGAGGCGTTTGCGCGGCATCAGG‑CATCTGAGCATGATGCCTg                                                      <  2:4745507/73‑1 (MQ=21)
                 aaaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTg                                                      >  1:1385833/1‑76 (MQ=255)
                 aaaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTg                                                      <  2:3762703/76‑1 (MQ=255)
                   aCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGAt                                                    <  2:3046192/76‑1 (MQ=255)
                   aCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGAt                                                    >  1:4140940/1‑76 (MQ=255)
                    ccTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATg                                                   >  1:5038850/1‑76 (MQ=255)
                    ccTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATg                                                   <  2:361986/76‑1 (MQ=255)
                      tGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcg                                                 <  2:2536995/76‑1 (MQ=255)
                      tGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcg                                                 <  1:2068705/76‑1 (MQ=255)
                      tGTTGAAATTATTGCTTTTGCAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATCATGCCCGAcgcg                                                 >  2:631029/1‑76 (MQ=18)
                       gTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcgg                                                >  1:3947456/1‑76 (MQ=255)
                        ttGAAATTATTGCTTTTGTAGGCCGGATAAGGAGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggc                                               >  2:4147463/1‑76 (MQ=25)
                         tGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcg                                              >  2:2102151/1‑76 (MQ=255)
                         tGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcg                                              <  1:344628/76‑1 (MQ=255)
                          gAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgc                                             <  2:2440166/76‑2 (MQ=255)
                          gAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgc                                             <  1:957522/76‑2 (MQ=255)
                           gaaTTGTTGTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgct                                            <  2:5684933/75‑3 (MQ=18)
                           aaaTTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgct                                            <  1:2332861/76‑3 (MQ=255)
                           aaaTTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgct                                            >  2:753531/1‑74 (MQ=255)
                           aaaTTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgct                                            <  1:1738880/76‑3 (MQ=255)
                                  tGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcgcg                                     <  1:4461072/76‑1 (MQ=18)
                                  tGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcgcg                                     <  1:460886/76‑1 (MQ=18)
                                   ggttttGTGGGGCGGATAAGGCGTTTGCGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGt                                    <  2:5323602/74‑1 (MQ=17)
                                    cTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTc                                   <  2:1273105/76‑1 (MQ=18)
                                       ttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCtca                                >  1:3056857/1‑74 (MQ=18)
                                       ttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTa                                <  2:4424094/76‑1 (MQ=18)
                                        tGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTAt                               >  1:3637006/1‑76 (MQ=18)
                                         gTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATc                              >  2:4395508/1‑76 (MQ=18)
                                           aGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAg                            >  1:1153518/1‑76 (MQ=17)
                                           aGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAg                            >  2:5162777/1‑76 (MQ=17)
                                             gCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCCGGTCGCGTCCTCTCCGGc                          >  2:680959/1‑76 (MQ=17)
                                              ccGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGcc                         >  2:5678996/1‑76 (MQ=17)
                                               cGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGccc                        >  2:1069928/1‑75 (MQ=14)
                                               cGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCt                        <  2:5187965/76‑1 (MQ=17)
                                               cGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCt                        >  1:3246367/1‑76 (MQ=17)
                                                ggATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTa                       >  1:790874/1‑76 (MQ=17)
                                                ggATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTa                       <  1:2170883/76‑1 (MQ=17)
                                                 gATGAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                      <  2:1057782/76‑1 (MQ=14)
                                                 gATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                      >  1:12309/1‑76 (MQ=17)
                                                 gATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                      >  1:1976523/1‑76 (MQ=17)
                                                 gATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                      <  1:4011746/76‑1 (MQ=17)
                                                   tAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGCTGCCTGATGCGGCGCTGGGCGCGTCTTATCAGGCCCACGa                    >  1:1242545/1‑76 (MQ=17)
                                                   tAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGa                    <  1:2467049/76‑1 (MQ=17)
                                                    aaGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGAc                   <  2:4892949/76‑1 (MQ=17)
                                                    aaGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGAc                   <  2:742438/76‑1 (MQ=17)
                                                     aGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACt                  <  2:3557190/76‑1 (MQ=17)
                                                     aGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACt                  >  1:381072/1‑76 (MQ=17)
                                                       gCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACttt                >  2:5241175/1‑76 (MQ=18)
                                                       gCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACttt                <  1:5298661/76‑1 (MQ=18)
                                                         gTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                      <  2:1429169/68‑1 (MQ=17)
                                                         gTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                      >  1:1429169/1‑68 (MQ=17)
                                                         gTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtct              >  2:1574908/1‑76 (MQ=18)
                                                           ttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTcc            >  1:4845867/1‑76 (MQ=18)
                                                           ttACGCAGCATAAGG‑CGTCTGAGCATGATGCCTGATGAGGCGCTGGTCGCGTATTATCAGGCCTAAGACTTTCTcc            <  2:1153518/76‑1 (MQ=16)
                                                            tACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCg           <  2:4042498/76‑1 (MQ=18)
                                                            tACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCg           >  1:4161388/1‑76 (MQ=21)
                                                             aCGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGTGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGc          >  2:2705991/1‑76 (MQ=21)
                                                             aCGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGc          >  1:3222830/1‑76 (MQ=21)
                                                              cgccggATCAGG‑CATCTGAGCATGATGCCTGAGGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCa         <  2:3002479/76‑1 (MQ=21)
                                                              cgccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCa         >  2:3212946/1‑76 (MQ=21)
                                                               gccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAt        >  2:915616/1‑76 (MQ=21)
                                                               gccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAt        <  1:4542714/76‑1 (MQ=21)
                                                               gccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAt        >  1:3170321/1‑76 (MQ=21)
                                                               cccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAt        <  1:1223799/75‑1 (MQ=21)
                                                                ccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATc       >  2:4045179/1‑76 (MQ=25)
                                                                 cgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATcc      >  1:5790573/1‑76 (MQ=25)
                                                                 cgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATcc      <  1:5753446/76‑1 (MQ=25)
                                                                  gcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCg     >  2:1887012/1‑76 (MQ=25)
                                                                  gcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCg     <  1:2894823/76‑1 (MQ=25)
                                                                   cATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg    >  2:110897/1‑76 (MQ=25)
                                                                   cATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg    <  1:5678998/76‑1 (MQ=25)
                                                                    aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc   >  2:5038566/1‑76 (MQ=25)
                                                                    aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGATCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc   <  2:4335093/76‑1 (MQ=18)
                                                                    aTCAGG‑CATCAGAGCATGATGCCTGATGCGGCGCTGGGCGCGTATTATCAGGCCTACGACTTTCTCCGACTCCGGc   >  2:5045465/1‑76 (MQ=18)
                                                                    aTCAGG‑CAGCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc   <  2:1867130/76‑1 (MQ=25)
                                                                     tCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCa  <  1:2421496/76‑1 (MQ=25)
                                                                          |                                                                       
TGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCA  >  NC_000913/4051069‑4051213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑