| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,085,675 | Δ1 bp | intergenic (+38/+13) | yiiG → / ← frvR | hypothetical protein/putative frv operon regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,085,675 | 0 | G | . | 98.2% | 475.1 / 1.1 | 108 | intergenic (+38/+13) | yiiG/frvR | hypothetical protein/putative frv operon regulatory protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (58/48); minor base A (2/0); total (60/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATC > NC_000913/4085604‑4085745 | cGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCg < 1:5621549/76‑1 (MQ=255)cGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCg < 1:5556230/76‑1 (MQ=255) aaaTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGac > 2:3272891/1‑76 (MQ=255) aaaTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGac < 2:5667106/76‑1 (MQ=255) aagggTTGATGACTATAATAGCCTGGGTTAAGCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGaca < 2:2789119/73‑1 (MQ=255) aaTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGaca < 2:2173042/76‑1 (MQ=255) aaTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGaca > 2:4601799/1‑76 (MQ=255) gTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGt < 1:2035252/76‑1 (MQ=255) gTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGt < 1:3101362/76‑1 (MQ=255) tgatgaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTATCGGATGCGGCGC‑ATCCGACACAGTTc < 1:2326755/76‑1 (MQ=255) tgatgaCTATAAGAGCCGGCGTTAAACGGGTGTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGCTc > 1:1396308/1‑76 (MQ=255) ggatgaATATAAGAGCCGGCGTTAAACGTTTTTGATGCTAAATGCAGTGTCGGCTGAGGCGC‑ATGCGACACAGtgc > 1:4384405/2‑74 (MQ=255) aCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAcccc > 2:2697043/1‑76 (MQ=255) aCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAcccc > 1:775509/1‑76 (MQ=255) aCTATAATAGCCTGCGTTAAACGCTTTTGCCGATAAATGCAGCGACGGACGCGGCGC‑ATCCGACACCGTTCAcccc > 2:2728804/1‑76 (MQ=255) cTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCt < 2:1551791/76‑1 (MQ=255) cTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCt > 2:4027949/1‑76 (MQ=255) cTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGCTCACCCCt > 1:943362/1‑76 (MQ=255) tataATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCtt < 1:1803712/76‑1 (MQ=255) ataataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTtg > 1:1083752/1‑76 (MQ=255) ataataGCCTGCGTTAAACGTTTTTAATGCTAAATGCAGTGTCGGATGCGGCTC‑ATCCGACACAGTTCACCCCTtg < 1:2249646/76‑1 (MQ=255) taataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTtgt < 2:3489690/76‑1 (MQ=255) taataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTtgt > 1:1873374/1‑76 (MQ=255) taataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTtgt < 1:5065525/76‑1 (MQ=255) taataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTtgt < 1:4009013/76‑1 (MQ=255) ataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGa > 2:4626803/1‑76 (MQ=255) taGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGac < 2:5411089/57‑1 (MQ=255) taGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGac > 1:5411090/1‑57 (MQ=255) taGCCTGCGTTAAACGTCTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGaa > 2:2101199/1‑76 (MQ=255) aGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAg < 1:1889400/76‑1 (MQ=255) aGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAg > 2:813824/1‑76 (MQ=255) gCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGt < 2:4627791/76‑1 (MQ=255) ccTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTa < 2:5148124/76‑1 (MQ=255) cTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTaa > 1:5443742/1‑76 (MQ=255) cTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGCGAAGTaa > 2:1192434/1‑76 (MQ=255) cTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCAACACAGTTCACCCCTTGTGAAGTaa > 2:4679395/1‑76 (MQ=255) tGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAAc > 1:389094/1‑76 (MQ=255) tGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAAc > 1:2760600/1‑76 (MQ=255) gCGTTAATCGTTTTTGTTGATAAATGCGGTGTCGGATCCGGCGC‑ATCCGACACCGTTCACCCCTTGTGAAGTAACt < 1:3388835/76‑1 (MQ=255) gCGTTAACCGTTTCTGATGATAAAGGCAGTGGCGGAGGCGGCGC‑ATCCGACACAGTTCGCCCCGTGTgtagtacct < 1:4988837/76‑4 (MQ=255) gCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACt < 1:375029/76‑1 (MQ=255) gTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTcc > 2:1292477/1‑76 (MQ=255) tAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCAt > 2:322541/1‑76 (MQ=255) tAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCAt > 1:1632042/1‑76 (MQ=255) aaaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCaca > 2:21333/1‑74 (MQ=255) aaaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATa > 2:2127579/1‑76 (MQ=255) aaaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCACTTGTGAAGTAACTCCATa > 2:2137337/1‑76 (MQ=255) aaCGTTTTTGATGATAAATGTAGTAGCGGCTGAGGCGC‑CTCCGCCACCGCTGACCCCTTGTGGCGTAATGCCATaa > 1:3740138/1‑76 (MQ=255) cGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAAt < 2:1584253/76‑1 (MQ=255) cGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAAt > 2:3839377/1‑76 (MQ=255) cGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAAt < 1:1099643/76‑1 (MQ=255) cGTTTTTGATGATAAATGCAGTGTCGGATGCGGCG‑AATCCGACACAGTTCACCCCTTGTGAAGCAACTCAATAAAt > 1:2666957/1‑76 (MQ=255) cGTTGTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAAt < 2:5475729/76‑1 (MQ=255) gTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATg > 2:1660763/1‑76 (MQ=255) tttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTCAAACCCTTGCGAAGTAACTCCATAAATGc > 2:301454/1‑76 (MQ=255) ttttGATGATACATGCAGTGTCGTATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGcc < 1:429422/76‑1 (MQ=255) ttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGcc > 1:141964/1‑76 (MQ=255) tttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCt > 2:5226386/1‑76 (MQ=255) tttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTGCACCCCCTGTGAAGGAACTCCATAAATcccc > 2:5204575/1‑75 (MQ=255) ttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTc < 2:5300543/76‑1 (MQ=255) ttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTc < 1:4435209/76‑1 (MQ=255) ttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTACACCCCTTGTGAAGTAACTCCATAAATGCCTc > 2:3601227/1‑76 (MQ=255) gatgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCat < 2:342709/76‑1 (MQ=255) gatgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCat < 1:3094715/76‑1 (MQ=255) atgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCata > 1:136307/1‑76 (MQ=255) tgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCctat > 2:948605/1‑76 (MQ=255) tgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCatat > 2:2965906/1‑76 (MQ=255) atAACTGCGGTGTCGGGGGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCatatgc < 2:1396308/76‑3 (MQ=255) atAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATcc < 1:2381099/76‑1 (MQ=255) tAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCt < 1:202409/76‑1 (MQ=255) aaTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGt > 2:3816697/1‑76 (MQ=255) aaTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGt > 1:5570829/1‑76 (MQ=255) aaTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGt > 1:4135639/1‑76 (MQ=255) aTGGAGTGGCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt < 2:849734/76‑1 (MQ=255) aTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt > 1:1748711/1‑76 (MQ=255) aTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt > 2:3872826/1‑76 (MQ=255) aTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt < 1:324004/76‑1 (MQ=255) gCAGTGTCGGATGCGTCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa < 2:3976863/76‑1 (MQ=255) gCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa > 2:5709100/1‑76 (MQ=255) gCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa > 1:4412827/1‑76 (MQ=255) aGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAcc < 1:2965906/76‑1 (MQ=255) gtgtCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAccc > 2:1881098/1‑76 (MQ=255) tgtCGGATGCGGAGC‑ATCCGACACAGGTCACCCCTTGTGAAGCAACTCCATAAATGCCTCATATCCTGTTAACCCg > 1:3011306/1‑76 (MQ=255) ggtCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCg < 2:3000722/75‑1 (MQ=255) gtCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTACCCCgc > 2:128593/1‑75 (MQ=255) ggcGGATGCGGCGC‑ATCCTACACAGTTCACCGCTTGTGAAGTATCTCCATAAATGCCTCATATCCTGTTAACCCgg < 2:4542542/74‑1 (MQ=255) tCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGc > 1:1944058/1‑76 (MQ=255) cGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCa < 1:396546/76‑1 (MQ=255) cGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCa < 1:2710343/76‑1 (MQ=255) cGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCa > 1:5205505/1‑76 (MQ=255) cGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCa > 1:3763019/1‑76 (MQ=255) ggATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaa > 2:5227193/1‑76 (MQ=255) ggATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaa > 2:5599568/1‑76 (MQ=255) ggATGCGGCG‑AATCCGAAACAGGTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaa > 2:3987871/1‑76 (MQ=255) gATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaat < 1:4952194/76‑1 (MQ=255) gATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaat > 2:4470956/1‑76 (MQ=255) tGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataa < 1:2295694/76‑1 (MQ=255) tGCGGCGC‑ATCCGACACAGTTCACCCCGTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataa < 1:2314694/76‑1 (MQ=255) cggcgC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtc < 1:5172254/76‑1 (MQ=255) cggcgC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtc < 2:3648993/76‑1 (MQ=255) | CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATC > NC_000913/4085604‑4085745 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |