| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,477,041 | Δ1 bp | intergenic (‑151/‑269) | b4256 ← / → yjgN | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,477,041 | 0 | G | . | 98.4% | 553.5 / ‑1.9 | 126 | intergenic (‑151/‑269) | b4256/yjgN | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base . (34/90); total (34/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 128 total aligned reads displayed.
CGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCC > NC_000913/4476971‑4477110 | ggccGGTGTGGCCTTGATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGAtt < 2:206190/73‑1 (MQ=255)cGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATACGGCGGATACCTGACGCATGACGCGGGCGATAGC‑AGGAtt > 2:1463704/1‑76 (MQ=255) gcGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTAt < 1:1090617/75‑1 (MQ=255) aCGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTAt < 1:5653419/76‑1 (MQ=255) ggTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATcc > 2:1700146/1‑76 (MQ=255) ggTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATcc > 1:4883828/1‑76 (MQ=255) gtgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCt < 1:4209125/76‑1 (MQ=255) gtgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCt < 2:2352158/76‑1 (MQ=255) tgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTg < 2:5151990/76‑1 (MQ=255) tgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTg < 1:2015057/76‑1 (MQ=255) gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑CGGATTATCCTGc > 2:31259/1‑76 (MQ=255) gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGc > 1:3046510/1‑76 (MQ=255) gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGc > 1:2094399/1‑76 (MQ=255) ggaGCCTTTATCAGCGGGAAGACCGTATTCGGCGGATACCTGGCGAATGACGCGGGCGATAGC‑AGGATTATCCTGc < 2:111314/74‑1 (MQ=255) tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCa < 1:1530096/76‑1 (MQ=255) tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCa > 1:3522800/1‑76 (MQ=255) tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCa < 1:346212/76‑1 (MQ=255) aGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAg < 1:2544058/76‑1 (MQ=255) gCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑CGGATTATCCTGCCGc > 2:172755/1‑76 (MQ=255) gCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGc < 2:3183671/76‑1 (MQ=255) gCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGc > 2:1640773/1‑76 (MQ=255) cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGt < 1:4950934/76‑1 (MQ=255) cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGt < 2:188528/76‑1 (MQ=255) tttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTc < 2:4680390/76‑1 (MQ=255) tttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTc > 2:4304749/1‑76 (MQ=255) tttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTc > 1:1397867/1‑76 (MQ=255) ttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCa < 2:1885762/76‑1 (MQ=255) tATCAGCGTTAAGGCCGTATTCGGCGGATCCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAg < 2:181139/76‑1 (MQ=255) tATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAg < 2:5310218/76‑1 (MQ=255) tATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAg < 2:3143974/76‑1 (MQ=255) tATCAGCGGTAAGACCGGATTCGGCGGATACCTGACGACTGACGCGGGCGATAGC‑AGGGTTATCCTGCAGCGTCAg > 2:706195/1‑76 (MQ=255) aTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAgg < 2:4858112/76‑1 (MQ=255) aTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAgg < 2:2759579/76‑1 (MQ=255) aTCACCGATAAGACCTTATACGGCGGATACCTGACGAATGCCGCGTGCTCTAGC‑AGGACTATCCTGCATCGTCAgg < 1:5044632/76‑1 (MQ=255) tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGc < 1:3148516/76‑1 (MQ=255) cAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcg < 1:2656795/76‑1 (MQ=255) cAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcg < 2:1213795/76‑1 (MQ=255) cAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcg < 2:80514/76‑1 (MQ=255) aGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcga > 1:3102997/1‑76 (MQ=255) cGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacg > 1:281892/1‑76 (MQ=255) ggTAAGACCGTATTCGGCGGATGCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgc < 1:2807350/76‑2 (MQ=255) ggTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgc > 1:788257/1‑75 (MQ=255) ggTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgc < 1:1022861/76‑2 (MQ=255) gACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAc > 2:3951259/1‑76 (MQ=255) ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAg < 2:2541291/76‑1 (MQ=255) ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAg > 2:2737224/1‑76 (MQ=255) ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAg < 2:3102997/76‑1 (MQ=255) gTATTCGGCGGATACCTGACGAATGACGCGGGTGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGt < 1:3850921/76‑1 (MQ=255) gTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGt > 2:2472888/1‑76 (MQ=255) gTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAAAGGt > 1:311865/1‑76 (MQ=255) tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTg < 1:5498250/76‑1 (MQ=255) ttCGGCGGATACCTGCCGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACATGTGAt < 1:1096466/76‑1 (MQ=255) ttCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAt < 1:5746444/76‑1 (MQ=255) gcggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAtt < 2:335454/75‑1 (MQ=255) cggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTg < 2:4170384/76‑1 (MQ=255) ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgc > 1:3839462/1‑76 (MQ=255) ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgc > 2:3260897/1‑76 (MQ=255) ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGCGACTgc > 2:1740792/1‑76 (MQ=255) aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGgcgcca < 2:1864855/76‑3 (MQ=255) aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCaa < 2:4883827/76‑1 (MQ=255) aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCaa < 1:3171742/76‑1 (MQ=255) ccTGACTAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAt < 1:2220056/76‑1 (MQ=255) ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAt < 1:19286/76‑1 (MQ=255) ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAt < 1:361005/76‑1 (MQ=255) ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAt < 1:262312/76‑1 (MQ=255) cTGACGAGTGACGCGGGCGATAG‑GAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATg < 2:3052689/76‑1 (MQ=255) cTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAt > 2:2335051/1‑64 (MQ=255) cTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAt < 1:2335051/64‑1 (MQ=255) cTGACGAATAACGCGGGCGGTAGC‑AGGATGATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATg < 2:3021201/76‑1 (MQ=255) gACGAATGACTCGTGCGTTAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGtt < 2:3651079/76‑1 (MQ=255) gACGAATGACGCGGGCGATAGC‑ATGATTATCCTGCGGCGTCAGGCGACGCATTGCAGGTGATTGCGGCGCAATGtt < 1:4607719/76‑1 (MQ=255) gACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgc > 2:5801250/1‑65 (MQ=255) gACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGtt < 1:5138595/76‑1 (MQ=255) aCGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTa < 1:1637162/76‑1 (MQ=255) ggAAGGACGGGGGCGATAG‑GAGGATTATCCTACAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAt < 2:5129799/75‑1 (MQ=255) gAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGCCGCATTACAGGTGATTGCGGCGCAATGTTAtt < 2:1076214/76‑1 (MQ=255) gAATGACGCGGGCGATAGC‑AGGAGTATCCTGCAGCGTCAGGCGCCGCATTGCTGGTGATTGTGGCGCGCTGTTAtt < 2:1122822/76‑1 (MQ=255) aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAttt > 1:1117532/1‑75 (MQ=255) aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTc < 2:281892/76‑1 (MQ=255) aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTc < 2:3852652/76‑1 (MQ=255) aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTc < 1:1900909/76‑1 (MQ=255) aTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCa > 1:1452537/1‑76 (MQ=255) tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAt < 1:5590518/76‑1 (MQ=255) tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAt < 1:664469/76‑1 (MQ=255) gACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATa < 1:3286893/76‑1 (MQ=255) gACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATa < 2:2101086/76‑1 (MQ=255) aCGCGGGCGATAGC‑AGGATTATCCTGCATCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAg < 2:3649993/76‑1 (MQ=255) cgcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt < 1:3951260/76‑1 (MQ=255) cgcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt < 1:5806381/76‑1 (MQ=255) cgcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt < 2:1414661/76‑1 (MQ=255) cgcgGGCGATAGC‑AGGATGATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt < 1:2640525/76‑1 (MQ=255) gcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGtt < 1:4553067/76‑1 (MQ=255) cgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCCGCGCAATGTTATTCATAGttt > 2:856091/1‑76 (MQ=255) ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa < 2:4423599/76‑1 (MQ=255) ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa < 1:3629538/76‑1 (MQ=255) ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa < 1:327221/76‑1 (MQ=255) gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAc < 1:250940/76‑1 (MQ=255) cGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAcc < 2:2100745/76‑1 (MQ=255) cGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAcc < 1:189223/76‑1 (MQ=255) gATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc < 2:1051811/76‑1 (MQ=255) | CGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCC > NC_000913/4476971‑4477110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |