| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,255,616 | Δ1 bp | intergenic (‑19/+312) | yhaO ← / ← yhaP | putative transport system permease protein/putative L‑serine dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,255,615 | 0 | G | . | 97.8% | 399.5 / 1.2 | 93 | intergenic (‑18/+313) | yhaO/yhaP | putative transport system permease protein/putative L‑serine dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base . (42/48); total (43/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAGGCACGACCCGCCGGGGTCGAAGGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTA > NC_000913/3255539‑3255683 | gtgtCAGTACTGTCGAATTTGATCGCTTCTCACCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAg < 1:1596466/76‑1 (MQ=255) tgtCAGTCCTGTCGAATTTGACCGCTTCTCTCCACTCGCGCTCGCTCATTCCA‑GCACGCCCCGCCGGGGTCGAAgc < 1:485840/76‑2 (MQ=255) gtCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAgcg > 1:4940898/1‑74 (MQ=255) gtCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAgcg > 1:3726105/1‑74 (MQ=255) cattACAGTCGAAGTGGAACACCTCCCGCCGCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCG‑AGGCGTc < 1:487869/73‑1 (MQ=255) aGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCt > 2:2961639/1‑76 (MQ=255) aGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCt > 1:3110281/1‑76 (MQ=255) aGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCt > 1:4319161/1‑76 (MQ=255) gTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTg > 1:1247932/1‑76 (MQ=255) gTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTg < 1:4986068/76‑1 (MQ=255) tACTGTCGATTTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGc < 1:2199397/76‑1 (MQ=255) aCTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCa < 1:5592475/76‑1 (MQ=255) tctCGAGTGTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAAt < 2:355273/74‑1 (MQ=255) tGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAAt > 2:1081212/1‑76 (MQ=255) tGTCGAATTTGACCGCTTCTCGCCTGTCGGTCTCACTCATTCCA‑GCGCGGCCCGCCTGGGTCGAA‑GCGTCTGCAAt < 2:353943/76‑1 (MQ=255) gTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATg > 1:3547386/1‑76 (MQ=255) gTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCG‑ACGCGTCTGCAATg > 2:269561/1‑76 (MQ=255) tCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGa < 2:589153/76‑1 (MQ=255) tCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGa > 1:2530645/1‑76 (MQ=255) cGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAc < 1:63218/76‑1 (MQ=255) gAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCGCGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACg < 2:5760498/76‑1 (MQ=255) actttGATCGCTTCTCGCATCTCGCTCTCACTCCTTCCA‑GCACGATACGGCGGGTTCTAA‑GCGTCTGCAATGACGc < 1:3924460/74‑1 (MQ=255) aaTTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGc < 1:3899594/76‑1 (MQ=255) aTTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGcc > 2:631749/1‑76 (MQ=255) aTTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGcc > 2:2756824/1‑76 (MQ=255) tGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCttt < 1:2861899/76‑1 (MQ=255) tGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCttt < 1:4772403/76‑1 (MQ=255) gagcGCTTCGCGCCACTCGCTCTCACTCATTCCA‑GCACGCCCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTa < 2:5263021/73‑1 (MQ=255) gATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTa > 2:3398796/1‑76 (MQ=255) gATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTa > 2:1066736/1‑76 (MQ=255) gATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTa > 1:1378449/1‑76 (MQ=255) gATCCCTCCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTa < 2:1084957/76‑1 (MQ=255) aTCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAt > 2:2170896/1‑76 (MQ=255) gCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCg > 2:4883370/1‑76 (MQ=255) gCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCg > 2:1098627/1‑76 (MQ=255) cTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGa > 1:2776116/1‑76 (MQ=255) ttCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGAt > 1:3979067/1‑76 (MQ=255) tctcGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATg < 2:4331413/76‑1 (MQ=255) tcGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGCTGCa > 1:3746763/1‑76 (MQ=255) tcGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCa < 2:2005729/76‑1 (MQ=255) tcGCCACTCGCTCCCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCa > 2:4249379/1‑76 (MQ=255) cGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCaa < 1:1219680/76‑1 (MQ=255) ccACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAAtt > 1:995031/1‑76 (MQ=255) cACTCGCTCTCACTCATTCCA‑GCACGCCCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAAttt < 2:3355933/76‑1 (MQ=255) cACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAAttt < 1:3381408/76‑1 (MQ=255) cACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAAttt > 2:1933776/1‑76 (MQ=255) ccTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTc < 1:1066736/75‑1 (MQ=255) ccTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTc < 1:657383/75‑1 (MQ=255) ccTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTc < 1:828991/75‑1 (MQ=255) cTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTcc > 1:5617110/1‑76 (MQ=255) cTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTcc > 1:1293475/1‑76 (MQ=255) gCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATa > 1:1796056/1‑76 (MQ=255) ctctCCCTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATaa < 2:5404519/76‑1 (MQ=255) ctctCACTCATTCCA‑GCGCGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATaa < 1:1817326/76‑1 (MQ=255) ctctCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATaa > 1:3018608/1‑76 (MQ=255) ctctCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATaa < 1:5370791/76‑1 (MQ=255) tctcACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAAt > 1:4150499/1‑76 (MQ=255) tctcACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAAt < 1:3334806/76‑1 (MQ=255) tctcACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCAATGCAATTTCCATAAt > 2:4919626/1‑76 (MQ=255) ctcacccaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAAtt > 1:4580703/1‑76 (MQ=255) tcactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCCTAAttt > 2:469718/1‑76 (MQ=255) tcactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAAttt > 2:2112486/1‑76 (MQ=255) tcactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAAttt < 2:2530645/76‑1 (MQ=255) cactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTtc > 1:3306062/1‑76 (MQ=255) actcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTtct < 2:3892989/76‑1 (MQ=255) ctcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTtctc < 2:180271/76‑1 (MQ=255) ctcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCCGCAATGACGCCTTTATTCGATGCAATTTCCATAATTtctc < 1:2803/76‑1 (MQ=255) tcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCg < 1:643058/76‑1 (MQ=255) caTTCCA‑GCACGACCCGCGGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGc < 1:2683082/76‑1 (MQ=255) ttCCA‑GCACGACGCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTc < 2:2919868/76‑1 (MQ=255) ttCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCCTTATTCGATGCAATTTCCATAATTTCTCCCTc > 2:5477760/1‑76 (MQ=255) ccagCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCaa < 1:5373632/73‑1 (MQ=255) agCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAata > 1:2525371/2‑76 (MQ=255) agCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAata < 2:4315114/75‑1 (MQ=255) gCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAatat < 1:5645730/76‑1 (MQ=255) gCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAatat > 2:1675352/1‑76 (MQ=255) cACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAtt < 1:628422/76‑1 (MQ=255) cACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAtt < 2:3619956/76‑1 (MQ=255) cACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAtt < 2:3726105/76‑1 (MQ=255) aCGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATaa > 1:1796047/1‑59 (MQ=255) aCGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATaa < 2:1796047/59‑1 (MQ=255) gACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTg < 1:580357/76‑1 (MQ=255) gACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTg > 2:562236/1‑76 (MQ=255) gACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTg < 2:1795220/76‑1 (MQ=255) cccGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTa < 2:3777147/76‑1 (MQ=255) cccGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTa > 2:2756463/1‑76 (MQ=255) ccgccgGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAg < 2:527941/76‑1 (MQ=255) cgccgGGGTTGCA‑GCGTCTGCAATGACGCGTTTATTCGATGCAATTTCCATAATTTTTCGCTCAATATTTTGTAgg < 2:2573937/76‑1 (MQ=255) gccgGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAggg > 2:523708/1‑76 (MQ=255) ccgGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGc < 1:3549037/76‑1 (MQ=255) cgGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCt > 2:2415694/1‑76 (MQ=255) cgGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCt > 2:1181858/1‑76 (MQ=255) cgGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCt < 1:3104072/76‑1 (MQ=255) ggggTGGAAGG‑GTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCtt < 2:144697/76‑1 (MQ=255) ggggTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCtt > 1:1732614/1‑76 (MQ=255) ggggTCGAA‑GCGTCTGAAATGACGCCTTTATTCGATGCAATTTCCATAAGTTCTCGCTCTATATTTTGTAGGGcgt > 1:3661789/1‑74 (MQ=255) gggTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCtta < 2:4236071/76‑1 (MQ=255) | GTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAGGCACGACCCGCCGGGGTCGAAGGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTA > NC_000913/3255539‑3255683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |