| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,429,527 | Δ1 bp | coding (40/510 nt) | yrdD ← | putative DNA topoisomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,429,526 | 0 | C | . | 99.2% | 570.6 / ‑2.8 | 126 | coding (41/510 nt) | yrdD | putative DNA topoisomerase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (57/68); minor base G (0/1); total (57/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 135 total aligned reads displayed.
GAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGC > NC_000913/3429451‑3429597 | gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATc > 2:4960402/1‑76 (MQ=255)gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATc < 1:1247920/76‑1 (MQ=255)gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATc > 1:5393719/1‑76 (MQ=255)gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATc < 2:2542434/76‑1 (MQ=255)gAAGATGTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATc < 2:2552103/76‑1 (MQ=255) aaGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcg < 2:667991/76‑2 (MQ=255) aGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcga < 1:5194658/76‑3 (MQ=255) gATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcgaa < 1:1095932/76‑4 (MQ=255) ttCAGAGTACTGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATaa < 1:2531550/76‑1 (MQ=255) agagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAccc > 2:1992579/1‑75 (MQ=255) gagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCa > 2:4954781/1‑75 (MQ=255) agGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGGT‑CGAATAACCAGt > 2:1536464/1‑76 (MQ=255) ggacggacGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGtt < 2:2036157/76‑1 (MQ=255) gacggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTc > 1:3973263/1‑76 (MQ=255) cctgacgTATTCACACGCCTGATACTGTGAGCATCCTAAAAACGGGCCGTGTTTCCCGGAT‑CGAATGACCAGTTCa < 1:3233994/73‑1 (MQ=255) acggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCa < 1:4612245/76‑1 (MQ=255) cgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcccc > 1:762728/1‑76 (MQ=255) cgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcccc < 2:2867148/76‑1 (MQ=255) cgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcccc < 1:3842315/76‑1 (MQ=255) gTAGTCGCACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCg < 2:523727/76‑1 (MQ=255) gTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCg > 1:237359/1‑76 (MQ=255) gTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCg > 1:182871/1‑76 (MQ=255) gTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGCGTTTCCCGGAT‑CGAATAACCAGTGCAGCCCCg > 2:3846989/1‑76 (MQ=255) gTAGTCACACGCCGGATACGGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTGCAGCCCCg > 2:1980622/1‑76 (MQ=255) tAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGCTCAGCCCCGc > 2:357061/1‑76 (MQ=255) tAGGCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGc < 2:589822/76‑1 (MQ=255) aGTCACACGCCGGATACTGTGAGCATCCAAGAAGCGTACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCa < 2:3813339/76‑1 (MQ=255) tCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACt > 2:4279236/1‑76 (MQ=255) cacaCGCCGGATACTGTGAGCATCCAAGAAACTGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACtt < 1:3055413/76‑1 (MQ=255) cacaCGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACtt < 1:4957296/76‑1 (MQ=255) ccacGCCGGATACTGTGCGCCTCCAAGAAACGGCCCGTGTTTCCCGGAT‑AGAATAACCAGTTCAGCCCCGCACttt > 2:4061487/2‑76 (MQ=255) ccacGCCGGATACTGTGAGCATCCAAGAAGCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCTCttt < 1:5828984/75‑1 (MQ=255) acacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACttt > 2:4009662/1‑76 (MQ=255) acacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACttt < 1:3505118/76‑1 (MQ=255) acacCCCGGCTAATGGGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACttt > 1:1025027/1‑76 (MQ=255) acGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg < 1:1890704/76‑1 (MQ=255) acGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg < 2:5005923/76‑1 (MQ=255) acGCCGGATACTGTGAGCATCCAAGAAAAGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg < 1:1836625/76‑1 (MQ=255) cGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTggg > 1:4633906/1‑76 (MQ=255) cGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTggg < 1:645875/76‑1 (MQ=255) cGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTggg > 1:3821575/1‑76 (MQ=255) gCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGc > 2:3272111/1‑76 (MQ=255) gCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGc < 2:5197831/76‑1 (MQ=255) ccGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCa < 2:3488429/76‑1 (MQ=255) ccGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCa < 1:1295847/76‑1 (MQ=255) cGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAg < 2:2260985/76‑1 (MQ=255) cGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAg > 1:4750581/1‑76 (MQ=255) cGGAGACAGTGAGCAGCCAATAAACGGACCGTGTTTCCAGGA‑GCGAATAACCAGTTCAGCCCCGCACTTTGGGCAg < 2:2314065/76‑1 (MQ=255) ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAgg > 2:1108883/1‑76 (MQ=255) gATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCCGGTCCGCCCCGCACTTTTGGCAggc > 2:563984/1‑75 (MQ=255) aTACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGCTCAGCCCCGCACTTTGGGCAGGAc < 1:357061/76‑1 (MQ=255) tACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGCT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACt > 1:3367395/1‑76 (MQ=255) tACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACt > 1:4795523/1‑76 (MQ=255) tACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACt > 1:3341591/1‑76 (MQ=255) tACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACt > 2:2436774/1‑76 (MQ=255) cTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCa < 1:5080535/76‑1 (MQ=255) tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCat < 2:2013387/76‑1 (MQ=255) tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCat < 2:4890292/76‑1 (MQ=255) tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCat < 2:5124121/76‑1 (MQ=255) tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCat > 1:1039692/1‑76 (MQ=255) tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCat > 2:296658/1‑76 (MQ=255) tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCat > 2:3338011/1‑76 (MQ=255) gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatt > 1:5684040/1‑76 (MQ=255) gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatt < 1:2279329/76‑1 (MQ=255) gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatt > 1:3892634/1‑76 (MQ=255) tgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatta > 1:1759738/1‑76 (MQ=255) gggCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCCCTTTGGGCAGGACTCattat < 2:5297772/74‑1 (MQ=255) gAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattat < 1:5653264/76‑1 (MQ=255) ggCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattatt < 2:4553113/75‑1 (MQ=255) gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAttatta < 1:1223951/76‑1 (MQ=255) gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAttatta < 2:5594895/76‑1 (MQ=255) cATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAc < 2:3321721/76‑1 (MQ=255) aTCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACg > 1:5312259/1‑76 (MQ=255) aGAAACGGACCGTGTTTCCCGGAT‑CGAATCACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCg > 2:29436/1‑76 (MQ=255) aGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCg < 1:3179959/76‑1 (MQ=255) aGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCg < 2:1441896/76‑1 (MQ=255) aaaCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTg > 1:1315654/1‑76 (MQ=255) aaCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGa < 1:1044146/76‑1 (MQ=255) aCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGGCTCATTATTACGCACCGTGaa > 1:5806064/1‑76 (MQ=255) gcccGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg < 2:5560122/74‑1 (MQ=255) gACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg > 1:2260398/1‑76 (MQ=255) gACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg < 1:1115451/76‑1 (MQ=255) aCCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGt < 1:3795918/76‑1 (MQ=255) aCCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGt < 1:2857405/76‑1 (MQ=255) ccGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCCCCGTGAACAGTg < 1:3363512/76‑1 (MQ=255) cGTGTTTCCCGGCT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCCCCGTGAACAGTGc < 1:1755406/76‑1 (MQ=255) gtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCt < 2:5338719/76‑1 (MQ=255) tgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTg > 2:4690392/1‑76 (MQ=255) tgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTg < 1:4279237/76‑1 (MQ=255) gtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGa < 2:3687894/76‑1 (MQ=255) gtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGa < 1:5600394/76‑1 (MQ=255) tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAt > 1:3494074/1‑76 (MQ=255) tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAt > 1:1589424/1‑76 (MQ=255) tCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAttt < 1:4009663/76‑1 (MQ=255) cccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTc < 1:4757635/76‑1 (MQ=255) ccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCg < 2:4952841/76‑1 (MQ=255) ccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCg < 1:4400757/76‑1 (MQ=255) ccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCg < 1:5153804/76‑1 (MQ=255) cGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGc > 1:3991875/1‑76 (MQ=255) cGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGc > 1:751097/1‑76 (MQ=255) | GAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGC > NC_000913/3429451‑3429597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |