| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,509,958 | Δ1 bp | intergenic (‑195/+314) | yhfZ ← / ← trpS | hypothetical protein/tryptophan tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,509,956 | 0 | T | . | 98.6% | 310.0 / ‑3.3 | 70 | intergenic (‑193/+316) | yhfZ/trpS | hypothetical protein/tryptophan tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (14/55); minor base A (1/0); total (15/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAA > NC_000913/3509881‑3510023 | cTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCct > 2:3045396/1‑76 (MQ=255) cATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCcttc < 2:772010/76‑2 (MQ=255) tATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCtttt < 2:2764360/76‑1 (MQ=255) tGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCtttttt < 1:5054252/76‑1 (MQ=255) ggTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTg < 1:3589416/76‑1 (MQ=255) gTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGa > 2:4891903/1‑76 (MQ=255) gTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGa > 1:5278612/1‑76 (MQ=255) ttttCAAGCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGAt < 2:5458196/76‑1 (MQ=255) tttCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATa < 1:335441/76‑1 (MQ=255) tttCAATCGATTACCGCAATTTTCAACCAACAAATAACGGGCCAGCGTCGTGATGACGACG‑CATTCTTGTTTGATa > 1:4496207/1‑76 (MQ=255) ttCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATaa < 1:3514960/76‑1 (MQ=255) ttCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATaa > 2:3956156/1‑76 (MQ=255) tCAATCGATTACCGCGCTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATaaa < 2:3374242/76‑1 (MQ=255) tCAATCGATTACCGCAGTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATaaa < 2:5099834/76‑1 (MQ=255) tCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATaaa < 2:4632780/76‑1 (MQ=255) cAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAAc < 2:4547560/76‑1 (MQ=255) cAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAAc < 1:1726544/76‑1 (MQ=255) aaTCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACg < 1:3702672/76‑1 (MQ=255) aaTCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACg < 1:3991962/76‑1 (MQ=255) aTCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGt < 2:5176224/76‑1 (MQ=255) tCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGtt < 1:5405125/76‑1 (MQ=255) cGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTc < 1:4296664/76‑1 (MQ=255) cGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTc < 1:5584524/76‑1 (MQ=255) aTTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGa < 2:44708/76‑1 (MQ=255) ttACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATACACGTTCGAc > 2:5417815/1‑76 (MQ=255) ttACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGAc < 1:3600680/76‑1 (MQ=255) ttACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGAc < 2:4502783/76‑1 (MQ=255) tACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACg > 2:5005608/1‑76 (MQ=255) tACCGCACTTTGCACCCAACAAATAACTGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACg < 1:3248862/76‑1 (MQ=255) tccGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGc < 1:5682238/75‑1 (MQ=255) cGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCat < 1:4143239/76‑1 (MQ=255) cGCACGTTTCCCCCAACAAACAACGGGCCAGCGGCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGCCGCat < 2:4858350/76‑1 (MQ=255) cTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCataata < 2:4806870/76‑1 (MQ=255) cTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCataata > 2:4052777/1‑76 (MQ=255) cTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCataata < 2:3787642/76‑1 (MQ=255) ttttCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCAtaataa < 2:5012032/76‑1 (MQ=255) tCACCCAACAAATAACGGTCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAAtt < 1:3336196/76‑1 (MQ=255) tCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAAtt > 1:2173935/1‑76 (MQ=255) ccAACAAATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCa < 2:401436/76‑1 (MQ=255) caacaaATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAg < 1:3125369/76‑1 (MQ=255) acaaATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTa < 1:3918895/76‑1 (MQ=255) caaATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTaa < 2:469531/76‑1 (MQ=255) caaATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTaa < 1:2472476/76‑1 (MQ=255) caaATAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTaa < 1:3056712/76‑1 (MQ=255) aaaTAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTaaa < 2:2097747/76‑1 (MQ=255) aaTAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAAt < 2:4477269/76‑1 (MQ=255) aaTAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGATCGACGCATAATAAATTTTCAGTAAAt < 2:2943591/76‑1 (MQ=255) aTAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAAtt > 1:3623376/1‑76 (MQ=255) tAACGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTg > 2:3920674/1‑76 (MQ=255) aaCGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGa < 1:2295198/76‑1 (MQ=255) cGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAat > 1:5098441/1‑76 (MQ=255) cGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAat < 1:5417085/76‑1 (MQ=255) cGGGCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAat > 1:3532045/1‑76 (MQ=255) gggCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAANNAATTTTCAGTAAATTGAata < 1:3523881/76‑1 (MQ=255) gCCAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATAtt < 1:968482/76‑1 (MQ=255) cAGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTta < 2:4289571/76‑1 (MQ=255) aGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCTACGCATAATAAATTTTCAGTAAATTGAATATTtat < 1:4731756/76‑1 (MQ=255) aGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTtat < 2:1741983/76‑1 (MQ=255) aGCGTCGTGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTtat < 1:846318/76‑1 (MQ=255) cgtGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCtt < 2:1772535/76‑1 (MQ=255) cgtGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCtt < 2:2220136/76‑1 (MQ=255) gtGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTc > 1:1738320/1‑76 (MQ=255) gtGATGACGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTc < 2:228312/76‑1 (MQ=255) tgatgaCGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCa < 2:2173935/76‑1 (MQ=255) tgatgaCGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCa > 1:918014/1‑76 (MQ=255) gatgaCGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAg < 2:5278611/76‑1 (MQ=255) gatgaCGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAg < 1:105481/76‑1 (MQ=255) gatgaCGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAg > 2:3290226/1‑76 (MQ=255) gatgaCGACACC‑TGCTTTGTGGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATGGATATCTTcct < 2:5321604/76‑3 (MQ=255) atgaCGACGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAgg < 2:2888727/76‑1 (MQ=255) gacgacGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGaa < 2:4496206/76‑1 (MQ=255) gacgacGCC‑TTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGaa < 2:3058811/76‑1 (MQ=255) | CTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAA > NC_000913/3509881‑3510023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |