Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,573,700 +C intergenic (+403/+997) yhgN → / ← gntK hypothetical protein/gluconokinase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,573,7001.C100.0% 435.4 / NA 171intergenic (+403/+997)yhgN/gntKhypothetical protein/gluconokinase 2
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (87/84);  total (87/84)

Only 100 of 182 total aligned reads displayed.

GAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCAC  >  NC_000913/3573625‑3573769
                                                                            |                                                                     
gAACCCTGCATGATGCGCACTGCAGCTGCCAACACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACg                                                                        <  2:2154414/76‑1 (MQ=255)
gAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACg                                                                        >  1:1314827/1‑76 (MQ=255)
 accccTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACgc                                                                       <  1:5183688/74‑2 (MQ=255)
   cccTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCCTGCCGGTTAACgcct                                                                     >  2:532946/1‑73 (MQ=255)
   cccTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACgctt                                                                     <  1:4253353/76‑4 (MQ=255)
      tGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGc                                                                  >  2:12363/1‑76 (MQ=255)
       gAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGcc                                                                 <  1:4127642/76‑1 (MQ=255)
         gtgatgGGCGCTGCAGCTGCCGGCAGGAAGCGGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCa                                                               <  2:257214/75‑1 (MQ=255)
         atgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCa                                                               >  1:3630977/1‑76 (MQ=255)
          tgatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAg                                                              <  1:3995429/76‑1 (MQ=255)
           gatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATCGCAATCGTCATGCCACTTCCGGTTAACGCTTCGCCCAGt                                                             <  1:3054048/76‑1 (MQ=255)
           gatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGt                                                             <  1:4148939/76‑1 (MQ=255)
           gatgCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGt                                                             <  2:1950579/76‑1 (MQ=255)
           gatgCGCACGGCAGCAGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAGCGCTTCGCCCAGt                                                             <  1:5481380/76‑1 (MQ=255)
              gcgcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCCGTGcc                                                          >  2:1421659/1‑76 (MQ=255)
               cgcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCg                                                         <  1:2181823/76‑1 (MQ=255)
               cgcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCg                                                         <  1:4925995/76‑1 (MQ=255)
                gcACTGCGGCTGCGAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCgg                                                        <  2:2587710/76‑1 (MQ=255)
                gcACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCgg                                                        <  2:1430861/76‑1 (MQ=255)
                 cACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGa                                                       <  1:628732/76‑1 (MQ=255)
                   cTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGAcc                                                     <  1:2672356/76‑1 (MQ=255)
                    tGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCt                                                    <  2:3750052/76‑1 (MQ=255)
                    tGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGGTAACGCTTCGCCCAGTGCCGGAccc                                                    >  1:442931/1‑75 (MQ=255)
                    tGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGGTAACGCTTCGCCCAGTGCCGGACCt                                                    <  1:3294202/76‑1 (MQ=255)
                     gCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCCGTGCCGGACCtc                                                   >  2:1043871/1‑75 (MQ=255)
                     gCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTa                                                   <  1:4797236/76‑1 (MQ=255)
                      cAGCTGCCAGCACGAAGCAGGTGCTATCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAc                                                  <  2:4051530/76‑1 (MQ=255)
                      cAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTAc                                                  <  1:1726463/76‑1 (MQ=255)
                       aGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACg                                                 >  1:2512797/1‑76 (MQ=255)
                        gCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGc                                                >  1:1328828/1‑76 (MQ=255)
                         cTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCCTGCCGGTTAACGCTTCCCCCAGTGCCGGACCTACGcc                                               >  2:2069232/1‑76 (MQ=255)
                          tGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCTCACGCCGGTTAACGCTTTGCCCAGTGCCGCACCTGCGCCa                                              >  1:3701065/1‑76 (MQ=255)
                          tGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCCTCGCCCACCCCCGGACCCACGccc                                              >  2:5534631/1‑75 (MQ=255)
                            ccAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCaga                                            <  1:5320223/76‑1 (MQ=255)
                            ccAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCaga                                            >  2:2294288/1‑76 (MQ=255)
                             cAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCCTGCCGGCTAACGCTTCGCCCAGCGCCGGACCTACGCCagag                                           >  1:2363626/1‑76 (MQ=255)
                             cAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCagag                                           <  2:757868/76‑1 (MQ=255)
                              aGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCTGTTACCTCTTCGCCCAGTGCCGGACCTACGCCagcgt                                          <  1:2168598/76‑4 (MQ=255)
                               gCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTc                                         <  2:5305637/76‑1 (MQ=255)
                               gCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTc                                         <  1:1494315/76‑1 (MQ=255)
                                cACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCGCAGTGCCGGACCTACGCCAGAGTCa                                        <  1:2054522/76‑1 (MQ=255)
                                cACGAAGCAGGTGATAGCAATCGGCAGGCCCAGGCCGGTCAACGCTGCGCCCAGTGCAGGACCTACGTCAGAGCCa                                        >  2:1646532/1‑76 (MQ=255)
                                  cGAGGCAGGTGATAGCAATCTGCGGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGCCCTACGCCAGAGTCAAc                                      <  1:5006953/76‑1 (MQ=255)
                                  cGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAc                                      <  2:3853427/76‑1 (MQ=255)
                                   gaggCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                     <  2:410345/73‑1 (MQ=255)
                                   gAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAAcc                                     >  2:3971644/1‑76 (MQ=255)
                                    aaGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTccacca                                    >  1:844573/1‑76 (MQ=255)
                                    aaGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCa                                    <  2:3628311/76‑1 (MQ=255)
                                      gCAGGTGATAGCAATCGGCAGGCCGATGCCGGTTATCGCTTCGCCCCGTGCCGGACCTACGCCAGAGTCATCCAGc                                  <  2:3038060/76‑1 (MQ=255)
                                      gCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGc                                  >  1:1198393/1‑76 (MQ=255)
                                      gCAAGTGATCGCAACCGGCAGGCCCATGCCGGTTAACGCTGCGCGAAGTGCCGTGCCTACGCCAGAGGCAACCAGc                                  <  2:391105/76‑1 (MQ=255)
                                       cAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCa                                 <  2:3487463/76‑1 (MQ=255)
                                       cAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCa                                 <  1:2358115/76‑1 (MQ=255)
                                        aGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAc                                >  2:2304071/1‑76 (MQ=255)
                                         ggTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCCGcccc                               >  2:4969806/1‑76 (MQ=255)
                                         ggTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCAcc                               <  1:4249465/76‑1 (MQ=255)
                                          gTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCt                              <  1:4549554/76‑1 (MQ=255)
                                          gTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCt                              <  2:4879127/76‑1 (MQ=255)
                                           tGATAGCAATCGGCAGGCGCATGCCGGTTAAAGCTTCGCCCAGCGCCGGAACTACGACAGAGTCTAAAAGCACCTg                             >  1:5608235/1‑76 (MQ=255)
                                           tGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTg                             <  1:2304071/76‑1 (MQ=255)
                                           tGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTg                             >  1:855471/1‑76 (MQ=255)
                                           tGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTg                             <  1:4920959/76‑1 (MQ=255)
                                              tAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGttt                          <  1:1088745/76‑1 (MQ=255)
                                              tAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGttt                          >  2:3350278/1‑76 (MQ=255)
                                               aGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCCGAGTCAACCAGCACCTGtttc                         >  2:5122415/1‑75 (MQ=255)
                                                gCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTCCGCCAGAGTCACCCAGCACCTGTTTGa                        >  1:3483118/1‑76 (MQ=255)
                                                 cAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGaa                       <  2:5165229/76‑1 (MQ=255)
                                                  aaTCGGCGGGCGCAGGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAac                      <  2:442931/76‑1 (MQ=255)
                                                  aaTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCCGCACCTGTTTGAac                      >  1:19317/1‑76 (MQ=255)
                                                  aaTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAac                      >  1:2575893/1‑76 (MQ=255)
                                                  aaTCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTCTGAac                      >  2:4269132/1‑76 (MQ=255)
                                                    tCGGCAGTCCCATGCCGGTTACCGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                    <  1:3661043/76‑1 (MQ=255)
                                                    tCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                    <  2:4607564/76‑1 (MQ=255)
                                                    tCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                    <  1:3547437/76‑1 (MQ=255)
                                                    tCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAacac                    >  2:4309400/1‑76 (MQ=255)
                                                     cGGGAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcg                   <  2:3057725/76‑1 (MQ=255)
                                                     cGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcg                   <  2:2466800/76‑1 (MQ=255)
                                                     cGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcg                   <  2:1065390/76‑1 (MQ=255)
                                                      ggCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcgc                  >  1:1615303/1‑76 (MQ=255)
                                                         aGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcgccgc               >  2:5243024/1‑76 (MQ=255)
                                                         aGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACAcgccgc               >  2:4303893/1‑76 (MQ=255)
                                                          ggCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACgccgcc              <  1:5012319/76‑1 (MQ=255)
                                                          ggCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACgccgcc              <  2:3664395/76‑1 (MQ=255)
                                                          ggCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACgccgcc              >  2:3404611/1‑76 (MQ=255)
                                                           gCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGGCCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgccc             >  1:707402/1‑76 (MQ=255)
                                                           gCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgccc             >  1:196983/1‑76 (MQ=255)
                                                            cccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGccgcccg            >  1:292863/1‑76 (MQ=255)
                                                               aTGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCAc         <  1:3949739/76‑1 (MQ=255)
                                                               aTGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCAc         >  1:3315660/1‑76 (MQ=255)
                                                               aTGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGGCAACCAGCAACTGTTTGAACACGCCGCCCGCAc         >  2:2887584/1‑76 (MQ=255)
                                                               aTGCCGGTTAACGCTTCGCCCAGGGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCAc         <  2:5245664/76‑1 (MQ=255)
                                                                tGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCAcc        >  2:4720018/1‑76 (MQ=255)
                                                                 gCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCa       <  1:221392/76‑1 (MQ=255)
                                                                  ccGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCat      >  1:5786235/1‑75 (MQ=255)
                                                                   cGGTTAACGCTTCGCCCAGTGCCGGACCTACGCAAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAAt     >  1:2490669/1‑76 (MQ=255)
                                                                    ggTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc    <  1:670805/76‑1 (MQ=255)
                                                                    ggTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATc    >  2:2917854/1‑76 (MQ=255)
                                                                     gTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCa   >  2:2745751/1‑76 (MQ=255)
                                                                      ttAACGCTTCGCCCAGTGCCTGACCTACGCCAGAGTCAACCAGCACCTGTGTGAACACGCCGCCCGCACCAATCAc  <  2:4503165/76‑1 (MQ=255)
                                                                      ttAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCACTCAc  >  2:4682038/1‑76 (MQ=255)
                                                                            |                                                                     
GAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACG‑TTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCAC  >  NC_000913/3573625‑3573769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑