| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,686,775 | +T | intergenic (‑23/+9) | b3530 ← / ← yhjM | putative oxidoreductase subunit/putative endoglucanase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,686,775 | 1 | . | T | 98.8% | 529.1 / ‑0.5 | 166 | intergenic (‑23/+9) | b3530/yhjM | putative oxidoreductase subunit/putative endoglucanase |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base T (76/86); total (80/86) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
Only 100 of 174 total aligned reads displayed.
ACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAA > NC_000913/3686700‑3686843 | aCTTACTCCAGAATCTGTTCCTGAGCGGTCGGTGCCACGTGGACCATCGGCATGACGGCCAGTCCGAGGGAAAGC‑g < 1:4869047/76‑1 (MQ=255) cTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gt < 1:4597423/76‑2 (MQ=255) ttGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtg > 2:2272529/1‑74 (MQ=255) ttGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtg > 1:4330866/1‑74 (MQ=255) tGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtga > 1:5781045/1‑73 (MQ=255) tGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtga < 2:2391060/76‑4 (MQ=255) tCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAata > 2:4128094/1‑76 (MQ=255) tCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAata > 1:2596544/1‑76 (MQ=255) tCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAata > 2:5211316/1‑76 (MQ=255) ccAGCAACTGTTGCTGGGCGGTGGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAatat < 2:2174193/76‑1 (MQ=255) ccAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAatat > 1:1473734/1‑76 (MQ=255) ccAGCAACTGTTGCTGAGCGGGTGGTGCTGCCGCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GAGAatat > 2:2432256/1‑76 (MQ=255) aGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGt < 2:4258897/76‑1 (MQ=255) aGCAACGGTGGCTGGGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGt < 2:2532502/76‑1 (MQ=255) gCATCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt < 1:5020464/76‑1 (MQ=255) gCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt < 1:3220206/76‑1 (MQ=255) gCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt > 2:2085655/1‑76 (MQ=255) gCAACTCTTGCTGAGCGGATGGTGCTGAATCGACCATCGGCAAGACCGCCAGAACGCGGGAAATC‑GTGTATATGtt > 1:3564422/1‑76 (MQ=255) gCAACGGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt < 2:3528819/76‑1 (MQ=255) cAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGttt > 1:3298057/1‑76 (MQ=255) cAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGttt > 1:4106399/1‑76 (MQ=255) aaCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTa > 2:3641974/1‑76 (MQ=255) aCTGTTGCTCAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAg > 1:2576740/1‑76 (MQ=255) cTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAgt < 2:1862412/76‑1 (MQ=255) tGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAgtg < 2:3751088/76‑1 (MQ=255) gTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAgtgt < 1:971552/76‑1 (MQ=255) tGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGa > 1:2177795/1‑76 (MQ=255) tGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGa < 1:4621605/76‑1 (MQ=255) gCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGaa > 1:951874/1‑76 (MQ=255) tGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGACAGC‑GTGACTATGTTTAGCGCGAAtt > 2:729247/1‑76 (MQ=255) gAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAAttt < 1:3845251/76‑1 (MQ=255) gAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACAGAGGGAAAGC‑GTGAATATGTTTAGTGTGAAttt > 1:1251407/1‑76 (MQ=255) cgCGGTTGGTACTGCCTCGTGCGTCGGGCTTACTTCCAGACCGAGGGAAAGC‑GTGACTATGTTTCGTGTGAGTTTg < 2:5024291/75‑1 (MQ=255) aGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTg > 1:1093022/1‑76 (MQ=255) gCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgc > 2:5315660/1‑76 (MQ=255) gCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgc < 1:3139486/76‑1 (MQ=255) cGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgcg < 1:289281/76‑1 (MQ=255) ggTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgcgn < 1:4412167/76‑2 (MQ=255) ggTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgcgc < 1:2262145/76‑1 (MQ=255) gTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCa < 2:3026199/76‑1 (MQ=255) ttGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGCGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCAt > 2:3367712/1‑76 (MQ=255) ttGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCAt > 2:3328281/1‑76 (MQ=255) ggTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTc < 1:2149124/76‑1 (MQ=255) ggTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTc < 1:1125095/76‑1 (MQ=255) gTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTcc < 2:1685039/76‑1 (MQ=255) tgctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCt > 1:514030/1‑76 (MQ=255) gctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTg < 1:2837618/76‑1 (MQ=255) gctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTg < 2:4478102/76‑1 (MQ=255) ctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTgg < 1:5211317/76‑1 (MQ=255) tgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGc > 1:4562278/1‑76 (MQ=255) tgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGc < 2:2092603/76‑1 (MQ=255) tgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGc > 1:2775912/1‑76 (MQ=255) ccTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGccc < 1:5586197/76‑1 (MQ=255) ccTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGccc > 2:4037417/1‑76 (MQ=255) cTCGACCATCGTCATGACGGCCATACCTAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGcccc < 1:3450655/76‑1 (MQ=255) cTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGcccc > 2:5674109/1‑76 (MQ=255) tCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCa > 1:1814296/1‑76 (MQ=255) tCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCa > 1:3981460/1‑76 (MQ=255) cGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAg > 2:126227/1‑76 (MQ=255) gACCATCGGCATGACGGCCAGACCGAGGGAGAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGt < 2:3653242/76‑1 (MQ=255) gACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAACTTGCGCATTCCTGGCCCCAgc > 2:4175215/1‑75 (MQ=255) aCCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTc > 1:2730653/1‑76 (MQ=255) ccATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCa < 1:3519289/76‑1 (MQ=255) aTCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAgg < 2:201555/76‑1 (MQ=255) aTCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAgg < 2:2699243/76‑1 (MQ=255) tCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGt > 1:751042/1‑76 (MQ=255) tCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGt < 2:2730653/76‑1 (MQ=255) cGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGta < 2:2438509/76‑1 (MQ=255) ggCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaa > 2:1441266/1‑76 (MQ=255) cATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaata > 2:2859019/1‑76 (MQ=255) aTGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaataa > 2:2622476/1‑76 (MQ=255) aTGACGGCCAGACAGAGAGAAAGCGG‑GAATTTGTTTCGTGTGAATTTGCGCATTCCGGGCTCCAGGCAGGtaataa > 1:1101790/1‑76 (MQ=255) tgccGGCCAGACCGAGGGAGAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAc < 1:478721/73‑1 (MQ=255) gACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACt > 1:2325213/1‑76 (MQ=255) gACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACt > 1:659173/1‑76 (MQ=255) aCGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTc < 1:68846/76‑1 (MQ=255) ggCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc > 1:377912/1‑76 (MQ=255) ggCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc > 2:4358136/1‑76 (MQ=255) ggCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc > 2:3615477/1‑76 (MQ=255) ggCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc < 1:4352829/76‑1 (MQ=255) gCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAcc > 1:1852963/1‑76 (MQ=255) gCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAcc < 2:2012965/76‑1 (MQ=255) ccAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCt < 2:3299396/76‑1 (MQ=255) ccAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCt > 2:2172097/1‑76 (MQ=255) ccAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCt < 2:1473734/76‑1 (MQ=255) cAGACCGAGGGAAAGC‑GTGAATATGCTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCtt < 2:3593545/76‑1 (MQ=255) aGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCttt > 1:2111698/1‑76 (MQ=255) aGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCttt < 1:83345/76‑1 (MQ=255) aCCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTg < 1:4683440/76‑1 (MQ=255) aCCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTg > 2:4941421/1‑76 (MQ=255) ccGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGt < 2:84753/76‑1 (MQ=255) gAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCg < 2:4553660/76‑1 (MQ=255) gAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCg < 2:3702658/76‑1 (MQ=255) aGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCga < 1:5315661/76‑1 (MQ=255) gggAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCgag < 2:4667693/76‑1 (MQ=255) ggAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCgaga > 1:3835430/1‑76 (MQ=255) gAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAGTAACTCACCTTTTGTCGAGaa > 1:5001070/1‑76 (MQ=255) gAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCgagca > 2:5423229/1‑74 (MQ=255) gAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGaa < 1:3003011/76‑1 (MQ=255) gAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGaa < 2:568375/76‑1 (MQ=255) | ACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAA > NC_000913/3686700‑3686843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |