| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,870,765 | +G | coding (476/1764 nt) | dgoA ← | 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,870,764 | 1 | . | G | 99.2% | 342.1 / ‑3.8 | 125 | coding (477/1764 nt) | dgoA | 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (58/66); total (58/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
TTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG > NC_000913/3870689‑3870834 | ttGCCCGGCGCGATAGCAATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑t < 1:731311/76‑1 (MQ=255) tGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑g < 1:3591457/76‑1 (MQ=255) tGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑g > 2:4546810/1‑76 (MQ=255) gCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑gg > 1:255656/1‑75 (MQ=255) cccGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑ggc > 1:470313/1‑74 (MQ=255) ccGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑ggcg < 1:2304156/76‑4 (MQ=255) gcgcGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCa > 1:2401292/1‑76 (MQ=255) gccgATAGAGGCCCCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAc < 2:243631/74‑1 (MQ=255) gccgATAGAGATCGCGCCATGAGCCCGCCTCTGTGCAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAc < 1:831113/74‑1 (MQ=255) cgcgATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAc < 1:5630502/76‑1 (MQ=255) gcgATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAcg < 1:2428346/76‑1 (MQ=255) gcgATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAcg > 1:1071826/1‑76 (MQ=255) tgATAGAGATCGGTTCCTAATCCCGCCCCTGCACAACCTCCGTCTATCCACGGCGCCAGGTTTTC‑TGGCGTCAcgc < 1:5050975/75‑1 (MQ=255) cgATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGCTTTC‑TGGCGTCAcgc > 2:2937933/1‑76 (MQ=255) gATAGGGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAcgcc < 2:5082564/76‑1 (MQ=255) gATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAcgcc < 1:491380/76‑1 (MQ=255) tAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAcgccgc < 1:253251/76‑1 (MQ=255) agagATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACgccgcc < 1:2268710/76‑1 (MQ=255) agagATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACgccgcc < 1:1621073/76‑1 (MQ=255) gagaTCGCTGCCTACGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCa < 2:4434132/76‑1 (MQ=255) gagaTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCa > 2:2806886/1‑76 (MQ=255) gagaTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCa > 2:4853596/1‑76 (MQ=255) agaTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCaa > 1:1691182/1‑76 (MQ=255) agaTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTCC‑TGGCGTCACGCCGCCaa > 1:5192540/1‑76 (MQ=255) gaTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAAc > 2:635103/1‑76 (MQ=255) aTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACg < 1:4870148/76‑1 (MQ=255) aTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACg > 1:2158220/1‑76 (MQ=255) cGCTGCCTACGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGc < 2:1146927/76‑1 (MQ=255) cGCTCCCTACGCCCCCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGc < 1:1263148/76‑1 (MQ=255) tGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCCCCAACGGCaaa > 2:766977/1‑76 (MQ=255) gCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAg > 1:5698598/1‑76 (MQ=255) ccTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGa < 2:4589678/76‑1 (MQ=255) cTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGAc < 1:1015185/76‑1 (MQ=255) tacgccggcccCTGCACAGCCTGCGTCTATCCACTGCGCCAGGTTTTC‑GGGCGTCACGCCGCCAACGGCAAAGACt < 2:508290/73‑1 (MQ=255) tAAGCCCGCCCCTGCACACCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACt < 2:867254/76‑1 (MQ=255) tAAGCCCGCCCCTGCACACCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACt < 1:1258814/76‑1 (MQ=255) accccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGc < 1:5237484/74‑1 (MQ=255) accccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGc < 1:1018905/74‑1 (MQ=255) aGCCCGCCCCTGCACACCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGc < 1:3564262/76‑1 (MQ=255) aGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGc < 2:5511589/76‑1 (MQ=255) gcccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCg > 1:5363068/1‑76 (MQ=255) ccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGc < 2:2357273/73‑1 (MQ=255) ccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGc > 1:2357273/1‑73 (MQ=255) ccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGAt > 2:4566318/1‑76 (MQ=255) ccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGAt < 1:2343182/76‑1 (MQ=255) ccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGAt < 2:1933154/76‑1 (MQ=255) cgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCACAGACTCCGATg > 2:5260132/1‑76 (MQ=255) gcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGt > 2:4726132/1‑76 (MQ=255) gcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGt > 1:3332671/1‑76 (MQ=255) gcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGt > 1:3272640/1‑76 (MQ=255) gcccCTCCACATCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGt < 1:5016922/76‑1 (MQ=255) ccccTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCCACGGCAACGACTGCGATgcc > 2:227879/1‑74 (MQ=255) cccTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTcc < 2:1733024/76‑1 (MQ=255) tGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGag > 1:810253/1‑75 (MQ=255) gcccaACCGGCGTCTACCCACTGCGCCAGGTTTTC‑TGGCGGCACGCCGCCAACGGCAAAGACTGCGATGTCCGATg < 2:5385071/73‑1 (MQ=255) gCACACGCTGCGTCTATCCTCTGCGCAGGATTTTCGG‑GCTCCACGCCGCCAACGGCAAAGACTTCGATGTCCGATg < 1:5219467/76‑1 (MQ=255) gCACAACCGGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATg < 2:3394060/76‑1 (MQ=255) cacaACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATgg < 1:3705313/76‑1 (MQ=255) acaACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGc > 2:2211461/1‑76 (MQ=255) atccTGCGTCTGGCCCCTGGGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCaa < 2:671032/74‑1 (MQ=255) aCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAAt < 1:2333255/76‑1 (MQ=255) ccTGCGTCTGTCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGGCCGATGGCAATa < 1:4800355/76‑1 (MQ=255) ccTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCACAGACTGCGATGTCCGATGGCAATa > 1:1179477/1‑76 (MQ=255) gCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCg < 1:3707530/76‑1 (MQ=255) gCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCg > 1:4294265/1‑76 (MQ=255) gCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCg < 1:4566319/76‑1 (MQ=255) gTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCt > 1:2222771/1‑76 (MQ=255) gTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCt < 2:2401292/76‑1 (MQ=255) gTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCt < 1:3737933/76‑1 (MQ=255) cTATACACTGCGCCAGGTTTTC‑TGGCGTAACGCCGCCAACGGCAAAGACTGCGATGGCCGATGGCAATACCGCttt > 1:2491287/1‑76 (MQ=255) tagccACTGCGCGAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCtttt < 2:742742/73‑1 (MQ=255) agccACGGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTACGATCGCAAGACCGACTTTa > 2:4268270/3‑76 (MQ=255) aTCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAACGACTGCGATGTCCGATAGCCATCCCGCTTTTa > 2:604124/1‑76 (MQ=255) aTCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTa < 1:4097674/76‑1 (MQ=255) tCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa > 1:3007504/1‑76 (MQ=255) tCCACGGCGCCGGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa < 2:4333812/76‑1 (MQ=255) ccACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAAc > 1:4286121/1‑76 (MQ=255) ccACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCCTTTAAc > 2:1238261/1‑76 (MQ=255) cACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACg > 2:4694729/1‑76 (MQ=255) cACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACg > 1:3826759/1‑76 (MQ=255) cACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACg < 2:4186274/76‑1 (MQ=255) cACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACg > 2:3453629/1‑76 (MQ=255) cACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACg < 1:5040694/76‑1 (MQ=255) aCTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGc > 1:3042330/1‑76 (MQ=255) aCTGCGCCAGGGTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGCTGACAATACCGCTTTTAACGc > 1:4319549/1‑76 (MQ=255) tGCGCCAGGTTTTC‑TGGCGTTACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTTACGCtt > 1:3118419/1‑76 (MQ=255) gcgcCAGGTTTTC‑TGGTGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCttt < 1:4554768/76‑1 (MQ=255) gcgcCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCttt > 1:68581/1‑76 (MQ=255) gcgcCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCttt > 2:297282/1‑76 (MQ=255) gcgcCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCttt < 1:4539934/76‑1 (MQ=255) cgcCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTg < 2:1718144/76‑1 (MQ=255) cgcCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACCCTTTg > 1:2372458/1‑76 (MQ=255) cAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATg < 1:407257/76‑1 (MQ=255) gggTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGt < 2:2793747/75‑1 (MQ=255) aGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGt > 2:5530307/1‑76 (MQ=255) ggTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTa < 1:1583287/76‑1 (MQ=255) ggTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTa > 1:1250291/1‑76 (MQ=255) gTTTTC‑TGGCGTCACGCCGCCAACGGCAAAAACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAt < 2:4717534/76‑1 (MQ=255) ttttC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAtt < 2:2261922/76‑1 (MQ=255) tttC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTg > 1:96966/1‑76 (MQ=255) | TTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG > NC_000913/3870689‑3870834 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |