| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,927,062 | +G | coding (2/1284 nt) coding (169/183 nt) |
yieM ← b4564 ← |
hypothetical protein hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,927,062 | 1 | . | G | 99.4% | 420.1 / ‑3.7 | 154 | coding (2/1284 nt) coding (169/183 nt) | yieM b4564 | hypothetical protein hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (72/81); total (72/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
Only 100 of 166 total aligned reads displayed.
AGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCA‑CGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGAC > NC_000913/3926987‑3927131 | agCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCa < 1:1291687/76‑1 (MQ=255) gCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCag > 2:2651374/1‑75 (MQ=255) gCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCag < 1:167361/76‑2 (MQ=255) cTGGCTTTGCTGATAGCACATCGCCTCTACGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCagc < 1:1328861/76‑3 (MQ=255) cTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTTCGCagc < 1:5121885/76‑3 (MQ=255) cTGGCTTTGCTGATAGCACATCACCTCGTCGGCGAGTTCCGGCGGGACTCGGGCAGCTTTCAGCCGACTGCGCagc > 1:5612602/1‑74 (MQ=255) tGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGACATCTTTCAGCCGACTGCGCagcg > 1:3324093/1‑73 (MQ=255) cTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCtt < 1:3324594/76‑1 (MQ=255) tttGCTGATAGCTCATCACCTCTTCGGTGCGTTCCGTCGGGACTCGGGCATCTTTCAGCCGACTGCTCAGCGCTTc < 1:1494766/76‑1 (MQ=255) tttGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTc < 1:481189/76‑1 (MQ=255) tttGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTc < 1:5593827/76‑1 (MQ=255) tttGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTc < 2:2656845/76‑1 (MQ=255) ttGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCa > 1:4205736/1‑76 (MQ=255) tGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCAc < 1:5066046/76‑1 (MQ=255) cTGATAGCACATCCCCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGc < 1:239382/76‑1 (MQ=255) tGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGcc < 1:734773/76‑1 (MQ=255) gATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCa > 1:1917621/1‑76 (MQ=255) tAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGc > 1:4329585/1‑76 (MQ=255) tAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGc < 1:4498127/76‑1 (MQ=255) tAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGc < 2:5311436/76‑1 (MQ=255) gCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCgg < 1:4402130/76‑1 (MQ=255) acaTCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCgggg > 1:1076499/1‑76 (MQ=255) aTCCCCGCTTCGGTGAGTTCGGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGaa < 2:1917621/76‑1 (MQ=255) aTCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCCGCggggga > 1:322506/1‑74 (MQ=255) aTCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCCGCGGGGaa > 2:1008540/1‑76 (MQ=255) aTCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGaa < 1:2060202/76‑1 (MQ=255) aTCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGaa > 2:2189818/1‑76 (MQ=255) aTCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGAGCAGCGCTTCACGCCAGCGGGGaa > 1:2991178/1‑76 (MQ=255) tctccTCTTCGGTGCGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCTCTTCACGCCAGCGGGGAAc < 1:738122/73‑1 (MQ=255) cACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAca > 2:5306386/1‑76 (MQ=255) cACCGCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAca < 2:73024/76‑1 (MQ=255) cccTCTTTGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAcat < 2:3955646/75‑1 (MQ=255) cccTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAcat < 2:4369299/75‑1 (MQ=255) acgtctcCGGTGAGTGCCGGCGGGACTCGTGCATCTTTCAGCCGACTGCGCAGCGCGTCACGCCAGCGGGGAAcat < 2:4406770/73‑1 (MQ=255) aCCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAcat < 2:5612600/76‑1 (MQ=255) aCCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAcat > 2:4540461/1‑76 (MQ=255) ctctTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCAAGCGGGGAAcatca < 1:5195418/76‑1 (MQ=255) ctTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACatcatc > 2:817930/1‑76 (MQ=255) ctTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACatcatc < 1:1431519/76‑1 (MQ=255) ttCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACAtcatca > 2:4230540/1‑76 (MQ=255) tCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAg > 1:4953098/1‑76 (MQ=255) cGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGt > 1:5387208/1‑76 (MQ=255) ggTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTg > 1:4894078/1‑76 (MQ=255) gTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGa > 1:1975129/1‑76 (MQ=255) tGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGAt < 2:3060021/76‑1 (MQ=255) gAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGAtt > 1:2906225/1‑76 (MQ=255) gTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgc < 2:1026986/76‑1 (MQ=255) tCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgcca < 2:2805454/76‑2 (MQ=255) ccGGGGGGACTCGGGGATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgccgc < 2:5460878/76‑1 (MQ=255) ccGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgccgc > 1:2782527/1‑76 (MQ=255) cggcggGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgccgcc < 1:201262/76‑1 (MQ=255) cggcggGACTCGCTCATCTTTCAGCCGGCTGCGCAGCGCTTCACGCGAGCGGGGAACATCATCAGTGATTgccgcc < 1:2824220/76‑1 (MQ=255) ggcggGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCt < 1:1734700/76‑1 (MQ=255) cggGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTc < 2:4323039/76‑1 (MQ=255) cggGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTc > 1:3826581/1‑76 (MQ=255) gggACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCa < 1:41452/76‑1 (MQ=255) gggACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCa < 2:2906225/76‑1 (MQ=255) gggACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCa > 1:1130805/1‑76 (MQ=255) ggACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAg < 2:4630509/76‑1 (MQ=255) aCTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGc < 2:3693505/76‑1 (MQ=255) cTCGGTCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCg < 2:4790411/76‑1 (MQ=255) tCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGt < 2:3778477/76‑1 (MQ=255) cGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTg > 1:1052710/1‑76 (MQ=255) cGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTg > 1:5101329/1‑76 (MQ=255) gggCATCTTTCAGCCGACGTCGCAGCGCTTCCCGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTgg < 2:291632/76‑1 (MQ=255) gggCAGCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTgg < 2:5653076/76‑1 (MQ=255) ggCATCTTTCAGCCGACTGCGCAGAGATTCACGCAAGCGGGGACCATCATCAGTGATTGCCGCCTTCCGGCGttgg < 1:4812927/76‑4 (MQ=255) gCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGa < 1:1206598/76‑1 (MQ=255) gCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGa > 2:4307212/1‑76 (MQ=255) gCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGa < 2:495401/76‑1 (MQ=255) cATCTTTCCGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGACTTCCGGCGTGGGaa > 1:1840060/1‑76 (MQ=255) cATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGaa > 1:1836427/1‑76 (MQ=255) cATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGaa > 1:2430810/1‑76 (MQ=255) cATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATCGCCGCCTTCAGGTGTGGGaa > 2:606059/1‑76 (MQ=255) cATCTTTCAGCCGACCGCGCAGCGCTTCACGCCAGCGGGGAACAGCATCAGTGATTGCCGCCTTCAGGCGTgggca > 2:3191409/1‑74 (MQ=255) cATCTCCCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACAGCAGCAGTGAGTGCCGCCTTCCGGCGTgggca > 2:3167320/1‑74 (MQ=255) gtCTTCCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAAt < 1:91303/75‑1 (MQ=255) tttCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAAtttt > 1:41225/1‑76 (MQ=255) tttCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAAtttt < 1:4952449/76‑1 (MQ=255) ttCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAAttttt > 2:2510732/1‑76 (MQ=255) tCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCCGGCGTGGGAATTTTTc > 2:5028202/1‑76 (MQ=255) tCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTc > 2:1368161/1‑76 (MQ=255) cAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCa < 2:4205735/76‑1 (MQ=255) cAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCa > 1:3650437/1‑76 (MQ=255) aGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCaa > 1:4711861/1‑76 (MQ=255) aGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCaa > 2:724394/1‑76 (MQ=255) aGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCaa < 2:2430810/76‑1 (MQ=255) gCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCaaa < 1:5318811/76‑1 (MQ=255) ccGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaag < 2:4953097/76‑1 (MQ=255) ccGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaag < 1:1120494/76‑1 (MQ=255) cGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaaga > 1:2134524/1‑76 (MQ=255) cGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaaga > 2:2456571/1‑76 (MQ=255) gACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAAtt > 1:4705552/1‑66 (MQ=255) gACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaagaa > 1:4420660/1‑76 (MQ=255) gcTGCGCAGCGCTTCACGCCAGGGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaagaag < 1:2249877/75‑1 (MQ=255) aCTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaagaag < 1:3710353/76‑1 (MQ=255) tGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGAc > 1:341696/1‑76 (MQ=255) tGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGAc > 1:5722745/1‑76 (MQ=255) tGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGAc > 2:3583043/1‑76 (MQ=255) tGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGAc < 2:1262330/76‑1 (MQ=255) | AGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCA‑CGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGAC > NC_000913/3926987‑3927131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |