| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,007,907 | +GG | intergenic (+109/‑11) | pldB → / → b4555 | lysophospholipase L(2)/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,007,907 | 1 | . | G | 96.5% | 299.0 / 5.0 | 114 | intergenic (+109/‑11) | pldB/b4555 | lysophospholipase L(2)/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base G (60/50); total (61/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 4,007,907 | 2 | . | G | 96.4% | 294.9 / 5.0 | 113 | intergenic (+109/‑11) | pldB/b4555 | lysophospholipase L(2)/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base G (59/49); total (60/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 127 total aligned reads displayed.
AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTA > NC_000913/4007832‑4007978 || aGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgc‑‑gcgc < 2:5257995/76‑1 (MQ=255)aGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgc‑‑gcgc < 2:693761/76‑1 (MQ=255) gATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgc‑‑gcgcg > 1:4936989/1‑75 (MQ=255) gATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgc‑‑gcgcg < 1:3648541/76‑2 (MQ=255) ttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACt > 2:3135064/1‑76 (MQ=255) tACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACtt > 2:3508880/1‑76 (MQ=255) aCTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACttt > 1:2277270/1‑76 (MQ=255) cTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTCtg > 2:1044842/1‑76 (MQ=255) tttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTtgt > 1:4728416/1‑76 (MQ=255) ttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTtgtg > 1:5711080/1‑76 (MQ=255) ttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTtgtg > 2:3110760/1‑76 (MQ=255) tctcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTgtgt > 1:1161463/1‑76 (MQ=255) ctcCCGACCATACGTTATCCCCTTACGCAAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGtt < 1:4146484/76‑1 (MQ=255) tcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGttt < 2:4491958/76‑1 (MQ=255) ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGc > 2:5609480/1‑76 (MQ=255) ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGc < 1:1672332/76‑1 (MQ=255) ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGc > 2:5167519/1‑76 (MQ=255) ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGc > 1:3239327/1‑76 (MQ=255) ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGc < 2:4281528/76‑1 (MQ=255) ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGc < 1:3612559/76‑1 (MQ=255) ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGc < 1:3832651/76‑1 (MQ=255) cGACCATACGTTATCCCCTTACGCCAAAGAAGCTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCg < 2:4725290/76‑1 (MQ=255) cGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCg < 1:1172553/76‑1 (MQ=255) cGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTATTTGCg < 1:1888208/76‑1 (MQ=255) gACCATACGTTATCCCCTTACGCCAACGAACCTATGCAGCCGCTCACCGC‑‑GCGCGGCACACACTTTGTGTCTGCGa > 2:708115/1‑76 (MQ=255) gACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgc‑‑gcgcg > 1:3142135/1‑54 (MQ=255) gACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgc‑‑gcgcg < 2:3142135/55‑2 (MQ=255) gACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGa > 2:3249576/1‑76 (MQ=255) gACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGa > 2:705416/1‑76 (MQ=255) aCCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGAc < 1:1013165/76‑1 (MQ=255) ccATACGTTATCCCCTTGCGCCAAAGAAACTCTGAAGCTTCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGAcc < 1:1803390/76‑1 (MQ=255) ccATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGAcc < 2:2587772/76‑1 (MQ=255) cATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCg < 2:638879/76‑1 (MQ=255) cATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCg > 1:2717372/1‑76 (MQ=255) aTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCgg > 2:4452639/1‑76 (MQ=255) aCGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGgtc > 2:1607623/1‑76 (MQ=255) ctttATCCCCTAACACCAAAGAAACCCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGgtcg < 1:1181590/74‑1 (MQ=255) cGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGgtcg < 1:4691496/76‑1 (MQ=255) cGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGgtcg > 2:923065/1‑76 (MQ=255) cGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGgtcg < 1:5613866/76‑1 (MQ=255) gTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGgtcgt > 2:3228970/1‑76 (MQ=255) tcaTCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGgtcgtc < 1:3879513/74‑1 (MQ=255) tATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGACATCAACTTTGTGTTTGCGACCGGTCGTca < 1:5025726/76‑1 (MQ=255) aTCCCGTTACTCCAAATAAACTCGGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcac < 1:891211/76‑1 (MQ=255) aTCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcac > 2:2740586/1‑76 (MQ=255) tCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcacc < 1:1555264/76‑1 (MQ=255) cccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcaccac < 1:3904894/76‑1 (MQ=255) cccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcaccac > 1:4808835/1‑76 (MQ=255) cccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcaccac < 2:4540476/76‑1 (MQ=255) ccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCCCCCCg > 2:5571746/1‑76 (MQ=255) ccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACg < 1:5616636/76‑1 (MQ=255) ttACGCCACAGAAGCGCTGAAGCTGCTCACCGC‑‑TCGCGGCATCCACTTTGTGTTTTCGACCGGTCGTCACCACGtt < 1:1692217/76‑1 (MQ=255) ttACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGtt < 1:2985346/76‑1 (MQ=255) ttACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGtt < 1:4951167/76‑1 (MQ=255) tACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACCTTg > 2:5595691/1‑76 (MQ=255) gCCAAAGAAACTCTGAGGCTGCTCCCCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtg < 2:4718824/76‑1 (MQ=255) gCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtg > 2:4310697/1‑76 (MQ=255) ccAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGCCGTCACCCCGTTGAcgt > 2:75312/1‑76 (MQ=255) cAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgtg > 2:3293098/1‑76 (MQ=255) aaGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTggg < 1:4452640/76‑1 (MQ=255) aaGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTggg > 1:5753779/1‑76 (MQ=255) aGAAACTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgggg < 1:3245886/76‑1 (MQ=255) aaaCTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCa > 2:5768071/1‑76 (MQ=255) aaaCTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCa > 1:82223/1‑76 (MQ=255) aaCTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaa > 2:3608170/1‑76 (MQ=255) aCTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaaa > 2:2651185/1‑76 (MQ=255) aCTCTGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGCTGATCTGGGGCaaa > 2:400044/1‑76 (MQ=255) ctctGAAGCTGCTCCCCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAt < 2:3846632/76‑1 (MQ=255) ctctGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAt > 1:233913/1‑76 (MQ=255) ctctGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAt < 2:1332806/76‑1 (MQ=255) tctGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAtt > 2:3585187/1‑76 (MQ=255) tctGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAtt < 2:4281118/76‑1 (MQ=255) ctGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTc < 1:717836/76‑1 (MQ=255) ctGAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTc < 2:2188430/76‑1 (MQ=255) ctGAACCTCCTCGCCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCCCCACGTTGATGTGGGGCAAATTc < 1:417847/76‑1 (MQ=255) gAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGTGGCAAATTcgc > 2:3528659/1‑76 (MQ=255) gAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgc > 1:597732/1‑76 (MQ=255) gAAGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgc < 1:2160434/76‑1 (MQ=255) gAACCTGCTCACCGC‑‑GCGCGGCATCAGCTTTGTGTTTGCGACCGGTCGACACCACGTTGATGTGGGGCAAATTcgc < 1:4244678/76‑1 (MQ=255) aaGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgcg > 1:4773220/1‑76 (MQ=255) aGCTGCTCCCCGCCGGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGa < 2:500039/76‑1 (MQ=255) aGCTGCTCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGa > 1:2575441/1‑76 (MQ=255) gctgctCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCCAATTCGCGac > 2:94958/1‑75 (MQ=255) gctgctCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAt > 2:3546002/1‑76 (MQ=255) tgctCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATaa > 2:3883095/1‑76 (MQ=255) tgctCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTACTCACCACGGTGATGAGGGGCAAATTCGCGATaa > 1:2944984/1‑76 (MQ=255) gctCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAt > 2:2219302/1‑76 (MQ=255) gctCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAt > 1:3615301/1‑76 (MQ=255) ctCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgggg > 1:5499438/1‑60 (MQ=255) ctCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATc > 1:281797/1‑76 (MQ=255) ctCACCGC‑‑GCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATc > 1:2123268/1‑76 (MQ=255) cgcgc‑‑gcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGgag < 1:4628938/74‑1 (MQ=255) gcgc‑‑gcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGgaga > 2:3267445/2‑76 (MQ=255) cgc‑‑gcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAt < 1:5768072/76‑1 (MQ=255) gc‑‑gcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGCGAtt > 2:4207108/1‑76 (MQ=255) gc‑‑gcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAtt < 2:2575441/76‑1 (MQ=255) gc‑‑gcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAtt < 1:3207490/76‑1 (MQ=255) gc‑‑gcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCAACACGTTGATGTGCGGCAAATTCGCGATAATCTGGAGAtg > 2:4610795/1‑75 (MQ=255) c‑‑gcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTa > 2:5489158/1‑76 (MQ=255) c‑‑gcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTa > 1:2542497/1‑76 (MQ=255) || AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGC‑‑GCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTA > NC_000913/4007832‑4007978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |