| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,008,473 | +G | intergenic (+40/‑193) | b4555 → / → yigM | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,008,472 | 1 | . | G | 96.5% | 223.5 / 2.6 | 87 | intergenic (+39/‑194) | b4555/yigM | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (50/33); total (52/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GC‑GGG‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAA > NC_000913/4008403‑4008541 | ggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GC‑GGG‑‑gaaag > 2:915022/1‑74 (MQ=255) ccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCAtt < 1:1872596/75‑1 (MQ=255) cTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTa > 1:3886085/1‑76 (MQ=255) tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTAt > 1:4499619/1‑76 (MQ=255) tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTAt < 1:2935877/76‑1 (MQ=255) gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATg > 1:1245432/1‑76 (MQ=255) gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATg > 1:1065087/1‑76 (MQ=255) aaGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATgg < 1:3820759/76‑1 (MQ=255) aaGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATgg < 1:4232602/76‑1 (MQ=255) aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATggg < 1:2548493/76‑1 (MQ=255) aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATggg > 2:3253005/1‑76 (MQ=255) aGGATTGTATTGCGTTTGGCGACGGGCTGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTCTggg > 1:5543071/1‑76 (MQ=255) ggATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGc > 1:4593855/1‑76 (MQ=255) ggATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGc > 1:3112/1‑76 (MQ=255) ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCg < 2:1217182/73‑1 (MQ=255) ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCg > 1:3697610/4‑76 (MQ=255) tggtgACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGt > 1:2569580/3‑76 (MQ=255) ggtgACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTc > 1:5050634/2‑76 (MQ=255) ggtgACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTc > 2:3992761/2‑76 (MQ=255) ggtgACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTc < 1:3622319/75‑1 (MQ=255) ggggACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTc < 2:5529668/73‑1 (MQ=255) gtgACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCt < 1:4076250/76‑1 (MQ=255) tgACGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTg > 2:4333679/1‑76 (MQ=255) gaggggAGGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGa < 2:5394620/73‑1 (MQ=255) gaggggAGGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCAGTATGGGCAGTGCGCACCAGCGTCTGa < 2:5449293/73‑1 (MQ=255) aCGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACTAGCGTCTGaa > 1:2869631/1‑76 (MQ=255) aCGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGaa < 1:3406146/76‑1 (MQ=255) cGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGaaa > 1:1231634/1‑76 (MQ=255) cGGGATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGaaa < 1:4096738/76‑1 (MQ=255) gggATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCgcacca > 2:5655796/1‑65 (MQ=255) gggATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAg > 1:2080745/1‑76 (MQ=255) gggATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAg > 2:2069747/1‑76 (MQ=255) gggATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAg > 2:1092253/1‑76 (MQ=255) gggATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATAATGGGCAGTGCGCACCAGCGACTGAAAg > 2:3901876/1‑76 (MQ=255) ggATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGa > 2:1874076/1‑76 (MQ=255) ggATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGa < 2:5842576/76‑1 (MQ=255) ggATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGa > 2:3855996/1‑76 (MQ=255) gATGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAACG‑CTGCATTATGGGCAGCGCGCACCAGCGTCTGAAAGAc > 2:4254916/1‑76 (MQ=255) aTGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAcc > 1:1331522/1‑76 (MQ=255) aTGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAcc > 2:1230256/1‑76 (MQ=255) tGAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCt > 1:5779737/1‑76 (MQ=255) gggacgacgCCGAAATGCTGGCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCt < 2:5428302/73‑1 (MQ=255) gAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCtt > 1:4304521/1‑76 (MQ=255) gAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCtt > 1:3437078/1‑76 (MQ=255) gAACGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCtt > 2:3564124/1‑76 (MQ=255) gAACGACGCCGAAATGCTGTCGATG‑GC‑GGGGCAAG‑G‑CTGCATTATGGGCCGTGCGCCCCAGCGTCTGCAAGAcata > 2:3597522/1‑73 (MQ=255) aaCGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTc > 1:4959963/1‑76 (MQ=255) aaCGACGCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTc > 1:3855544/1‑76 (MQ=255) acgactCCTAAATGCGGTCGAGG‑GCGGGG‑‑AAAGG‑CTTCATTATGGCCAGTGCGCACCAGCGTCTGAAAGACCTTCa < 2:1062961/76‑1 (MQ=255) acgacgCCGAAATGCTGTCGATGNGG‑GGG‑‑NAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCa < 2:1046323/76‑1 (MQ=255) cgacgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt < 1:972753/76‑1 (MQ=255) cgacgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt > 2:2730353/1‑76 (MQ=255) cgacgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt > 2:1530832/1‑76 (MQ=255) cgacgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt > 1:5670577/1‑76 (MQ=255) gacgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc < 2:4309193/76‑1 (MQ=255) gacgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc > 1:1664774/1‑76 (MQ=255) gacgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCCTCATc > 2:4835137/1‑76 (MQ=255) gacgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGGCTGAAAGACCTTCATc > 1:3541422/1‑76 (MQ=255) acgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAtac > 2:3453286/1‑74 (MQ=255) acgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc > 1:1802523/1‑76 (MQ=255) acgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc > 2:3083447/1‑76 (MQ=255) cgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc < 1:1735251/76‑1 (MQ=255) cgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc < 1:5288584/76‑1 (MQ=255) cgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc > 1:3237918/1‑76 (MQ=255) cgCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGGGCGCACCAGCGTCTGAAAGACCTTCATccc > 1:3252514/1‑76 (MQ=255) gCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg < 2:1684409/76‑1 (MQ=255) gCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg > 1:4952937/1‑76 (MQ=255) gCCGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg > 1:300196/1‑76 (MQ=255) ccGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa > 1:579879/1‑76 (MQ=255) ccGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa < 2:4959962/76‑1 (MQ=255) cGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg > 1:993413/1‑76 (MQ=255) cGAAATGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg < 2:3867434/76‑1 (MQ=255) agaTGCTGTCGGTG‑GCGGGG‑‑AGA‑GTCTGCAGTAGGGTCAGTGCGCACCAGCGTGTGAAAGACCTTCAGCCCGAGcg < 1:236779/74‑2 (MQ=255) aaaTGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt < 1:3796540/76‑1 (MQ=255) aaaTGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt < 2:4472166/76‑1 (MQ=255) aaaTGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt < 1:198609/76‑1 (MQ=255) aaaTGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt < 2:857560/76‑1 (MQ=255) aaTGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑g < 2:2210680/76‑1 (MQ=255) aTGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg < 1:5734751/76‑2 (MQ=255) aTGCTGGCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg < 1:5739028/76‑2 (MQ=255) tGCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga > 1:1095076/1‑74 (MQ=255) gCTGTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa > 1:5112817/1‑73 (MQ=255) gTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg > 2:4588871/1‑76 (MQ=255) gTCGATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg < 1:586117/76‑1 (MQ=255) gATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt > 2:3665213/1‑76 (MQ=255) gATG‑GCGGGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGGGAtt > 2:613244/1‑76 (MQ=255) gATG‑GCGGGG‑‑AAA‑GTCTGCATTATGGGCAGTGCGCACGAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt < 1:4254917/76‑1 (MQ=255) gg‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa < 2:3582901/76‑1 (MQ=255) | GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATG‑GC‑GGG‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAA > NC_000913/4008403‑4008541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |