| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,008,525 | +G | intergenic (+92/‑141) | b4555 → / → yigM | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,008,524 | 1 | . | G | 97.1% | 279.3 / 2.7 | 105 | intergenic (+91/‑142) | b4555/yigM | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (45/57); total (46/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
AAATGCTGTCGATGGC‑GGGAAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑GAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT > NC_000913/4008451‑4008594 | aaaTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑t < 1:3796540/76‑1 (MQ=255)aaaTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑t < 1:198609/76‑1 (MQ=255)aaaTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑t < 2:4472166/76‑1 (MQ=255) aaTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑g < 2:2210680/76‑1 (MQ=255) aTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑gg < 1:5734751/76‑2 (MQ=255) aTGCTGGCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑gg < 1:5739028/76‑2 (MQ=255) tGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑gga > 1:1095076/1‑74 (MQ=255) gCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑ggaa > 1:5112817/1‑73 (MQ=255) gTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTg > 2:4588871/1‑76 (MQ=255) gTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTg < 1:586117/76‑1 (MQ=255) gATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGAtt > 2:3665213/1‑76 (MQ=255) gATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GGGAtt > 2:613244/1‑76 (MQ=255) gATGGCGGGGAAA‑GTCTGCATTATGGGCAGTGCGCACGAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGAtt < 1:4254917/76‑1 (MQ=255) ggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTaa < 2:3582901/76‑1 (MQ=255) aagg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGc < 1:4771256/73‑1 (MQ=255) gg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCcg > 2:1577797/2‑76 (MQ=255) cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCcgac > 2:4495085/1‑76 (MQ=255) cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCcgac > 2:611629/1‑76 (MQ=255) tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCcgacg < 2:1685317/76‑1 (MQ=255) tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCcgacg < 2:630936/76‑1 (MQ=255) gCATTGTGGGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCcgacga < 2:2203445/76‑1 (MQ=255) gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCcgacga < 2:4499618/76‑1 (MQ=255) gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCcgacga > 1:3900500/1‑76 (MQ=255) cATTGTGGGCAGGGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCgacgac < 2:2398661/76‑1 (MQ=255) cATTATGGGCGGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCgacgac < 1:1193410/76‑1 (MQ=255) aTTAGGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGacgacg < 2:1127557/76‑1 (MQ=255) ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGAcgc < 1:1039579/76‑1 (MQ=255) ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACCAATGCCGACGAcgc > 1:1646632/1‑76 (MQ=255) aTGGGCAGTGCGCACCAGCGTCTTAAAGACCTTCATCCCGAGA‑TGGAA‑GTGCTTGGTACTCATGCCGACGACGCgg > 1:826464/1‑76 (MQ=255) aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCgg > 1:3627305/1‑76 (MQ=255) aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCgg > 1:796250/1‑76 (MQ=255) tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGt < 2:5108407/76‑1 (MQ=255) tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGt < 2:5217497/76‑1 (MQ=255) gggggAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGt < 2:5512824/75‑1 (MQ=255) gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTg > 1:5805249/1‑76 (MQ=255) gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGcc < 1:4423770/76‑1 (MQ=255) gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGcc < 2:1802523/76‑1 (MQ=255) gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGcc < 2:3665473/76‑1 (MQ=255) gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt > 2:2395116/4‑76 (MQ=255) gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt < 2:5112816/73‑1 (MQ=255) gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt > 1:2558600/4‑76 (MQ=255) gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt < 2:5033649/73‑1 (MQ=255) gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt > 2:5119704/4‑76 (MQ=255) cgcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCAttt < 2:4245766/74‑2 (MQ=255) cgcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa < 2:3378304/74‑1 (MQ=255) cgcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa > 1:406217/3‑76 (MQ=255) gcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt > 1:151957/2‑76 (MQ=255) gcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt > 1:2023607/2‑76 (MQ=255) gcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt < 1:4835138/75‑1 (MQ=255) gcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt > 1:3737106/2‑76 (MQ=255) gcaccaGCGTCTGAAAGACCTTCATCCCGAGC‑T‑GGACGTGATTGGTACTAATGCCGACGACGCGGCGGCGCAatat > 1:3210242/2‑76 (MQ=255) caccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc < 1:592913/76‑1 (MQ=255) accaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGAcgc > 2:331115/1‑61 (MQ=255) accaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGAcgc < 1:331115/61‑1 (MQ=255) accaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt < 2:1231634/76‑1 (MQ=255) accaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt > 1:2633647/1‑76 (MQ=255) ccaGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg < 1:3253005/76‑1 (MQ=255) catcGTCTGAAAGAACTGCATGCCGAGC‑TGGAA‑GTGATGGGTACTAATGCCGACGACGCGGTGCCGCAGTATCTGc < 1:708077/73‑1 (MQ=255) caGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc > 1:998503/1‑76 (MQ=255) caGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc < 1:656935/76‑1 (MQ=255) aGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg < 2:1291780/76‑1 (MQ=255) aGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg < 1:2714591/76‑1 (MQ=255) aGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg > 2:1477723/1‑76 (MQ=255) aGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg < 1:5728964/76‑1 (MQ=255) aGCGTCGGAAAGACCTTCATCCCGAGCGG‑GAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg < 2:300196/76‑1 (MQ=255) gCGTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGt > 1:5397961/1‑76 (MQ=255) ggTCGGAAAGACGTTCATCCCGAGCGG‑GAA‑GTGATTGGTACTAATGGCGACGACGCGGTGCCGCATTATCTGCGTa < 2:3984805/75‑1 (MQ=255) gTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa > 2:3866383/1‑76 (MQ=255) gTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa > 2:1211432/1‑76 (MQ=255) gTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa < 2:2910483/76‑1 (MQ=255) gTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa < 2:2470735/76‑1 (MQ=255) gTCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa > 2:1300315/1‑76 (MQ=255) tCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa < 1:3281181/76‑1 (MQ=255) tCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa < 1:3866384/76‑1 (MQ=255) tCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa > 1:691296/1‑76 (MQ=255) tCTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa < 1:1030557/76‑1 (MQ=255) cTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc < 2:2468302/76‑1 (MQ=255) cTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc > 2:605930/1‑76 (MQ=255) cTGAAAGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTCCGTAAAc > 2:814847/1‑76 (MQ=255) aaaGACCTTCATCCCGCGC‑TGGAA‑GTGATTGTTACTAATGCCGACGACGCGGTGGCGCATTATCTGCGTAAActct < 2:1540706/76‑1 (MQ=255) aaaGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAActct > 2:2731555/1‑76 (MQ=255) aaaGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAActct < 1:1098420/76‑1 (MQ=255) aaaGACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAActct > 1:1910766/1‑76 (MQ=255) gACCTTCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAtt < 2:1232689/76‑1 (MQ=255) ccTTCATCCCGAGC‑TGGGA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa < 2:183120/76‑1 (MQ=255) ttCATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATc < 1:2730353/76‑1 (MQ=255) cATCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt < 1:5291803/76‑1 (MQ=255) tCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTaa < 2:5190475/76‑1 (MQ=255) tCCCGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTaa > 2:2142512/1‑76 (MQ=255) cccGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCGTTATCTGCGTAAACTCTATTTATCGTAAt < 1:915022/76‑1 (MQ=255) cccGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTCTCGTaac > 2:484839/1‑75 (MQ=255) cccGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt < 1:5071901/76‑1 (MQ=255) cccGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt < 2:5241837/76‑1 (MQ=255) cccGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt < 2:4188541/76‑1 (MQ=255) ccGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc < 2:2023607/76‑1 (MQ=255) ccGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc < 2:691296/76‑1 (MQ=255) cGAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCg < 1:3187925/76‑1 (MQ=255) gAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt < 1:3564290/76‑1 (MQ=255) gAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt < 1:5294509/76‑1 (MQ=255) gAGC‑TGGAA‑GTGATTGGTACTAATGCCGACGACGAGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt > 1:2566431/1‑76 (MQ=255) | AAATGCTGTCGATGGC‑GGGAAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑GAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT > NC_000913/4008451‑4008594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |