BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,013,177	+C	coding (543/999 nt)	ysgA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,013,177	1	.	C	100.0%	421.8 / NA	151	A181A (GCC→GCG)	ysgA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (82/69); total (82/69)

Only 100 of 151 total aligned reads displayed.

CTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCCG‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAG  >  NC_000913/4013109‑4013242

cTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTa                                                             >  2:3767112/1‑76 (MQ=255)

cTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTa                                                             >  2:2144442/1‑76 (MQ=255)

 tGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTaa                                                            >  1:4560931/1‑76 (MQ=255)

 tGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTaa                                                            >  2:5048601/1‑76 (MQ=255)

 tGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTaa                                                            <  1:1817517/76‑1 (MQ=255)

 tGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTaa                                                            <  1:3345932/76‑1 (MQ=255)

 tGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTaa                                                            >  1:2118439/1‑76 (MQ=255)

 tGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTaa                                                            <  1:2172062/76‑1 (MQ=255)

 tGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTaa                                                            <  1:2164933/76‑1 (MQ=255)

  ggCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAg                                                           >  1:1267879/1‑76 (MQ=255)

  ggCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAg                                                           >  2:3930822/1‑76 (MQ=255)

   gcgcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGa                                                          <  2:4119031/76‑1 (MQ=255)

   gcgcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGa                                                          >  2:5561915/1‑76 (MQ=255)

    cgcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGAt                                                         >  2:2998286/1‑76 (MQ=255)

    cgcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGAt                                                         >  2:4046883/1‑76 (MQ=255)

    cgcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGAt                                                         >  2:1081307/1‑76 (MQ=255)

     gcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATc                                                        <  1:5048602/76‑1 (MQ=255)

     gcAGGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATc                                                        <  2:4661258/76‑1 (MQ=255)

      cATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCg                                                       <  1:4051948/76‑1 (MQ=255)

      cATGGGTTCAACACGCTCCTGCGGAATGCTGGGATCCTGACCACCATATAAGCCGCGAACCCGCGCGATAAGATCg                                                       >  2:2176445/1‑76 (MQ=255)

       aTGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCgg                                                      >  1:1859270/1‑76 (MQ=255)

        tGGTTTCCACGCTCTCCTGGGGAATGCTGTTATCCTGCCCGCCATATAAGCCGAGAATCGGCGCGTTAAGATCGGt                                                     <  2:1664118/76‑1 (MQ=255)

         ggTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGtt                                                    <  2:5218839/76‑1 (MQ=255)

         ggTTTCAACGCTCTCCGGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGtt                                                    <  2:4475829/76‑1 (MQ=255)

          gTTTCAACGCTCTCCTGCGGAATGCTGTTCCCCCCCCCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTg                                                   >  2:3132825/1‑76 (MQ=255)

          gTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTg                                                   <  2:2307713/76‑1 (MQ=255)

          gTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTg                                                   >  2:1478567/1‑76 (MQ=255)

          gTTTCAACGCTCTCCTGCGGAAGGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTg                                                   <  1:5162441/76‑1 (MQ=255)

            ttCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCg                                                 <  1:874181/76‑1 (MQ=255)

            ttCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCg                                                 <  2:3357394/76‑1 (MQ=255)

              cAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGat                                               <  2:3870913/76‑1 (MQ=255)

               aaCGCTCTCCTGCGGAATGCTGTTATCCTGACCACCTTATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGata                                              <  2:2848595/76‑1 (MQ=255)

               aaCGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGata                                              <  1:3927952/76‑1 (MQ=255)

                 cGCTCTCCTGCGGACTGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATc                                            <  1:4130797/76‑1 (MQ=255)

                 cGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATc                                            <  2:1033031/76‑1 (MQ=255)

                 cGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATc                                            >  1:4861463/1‑76 (MQ=255)

                  gCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGCTAAGATCGGCTGCGATATCa                                           >  2:464432/1‑76 (MQ=255)

                   ctctCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTGGCGATATCaa                                          >  2:1662512/1‑76 (MQ=255)

                    tctcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAAc                                         >  2:868518/1‑76 (MQ=255)

                     ctcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACa                                        <  1:5029678/76‑1 (MQ=255)

                      tcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAg                                       >  2:1195073/1‑76 (MQ=255)

                      tcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAg                                       >  1:4854545/1‑76 (MQ=255)

                      tcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAg                                       >  1:3572199/1‑76 (MQ=255)

                      tcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAg                                       >  1:2295325/1‑76 (MQ=255)

                      tcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAg                                       >  1:5767920/1‑76 (MQ=255)

                      cccTGCGGAATGCTGTTATCCGGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAg                                       <  1:288799/75‑1 (MQ=255)

                       ccTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAgg                                      <  1:3411451/76‑1 (MQ=255)

                       ccTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAgg                                      <  1:4348306/76‑1 (MQ=255)

                        cTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgt                                     >  1:2128275/1‑76 (MQ=255)

                         tGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgtt                                    >  1:156685/1‑76 (MQ=255)

                          gCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttg                                   >  2:4362738/1‑76 (MQ=255)

                           cGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttgt                                  >  1:3765580/1‑76 (MQ=255)

                            ggAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttgtt                                 >  2:4818415/1‑76 (MQ=255)

                            ggAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttgtt                                 >  1:2738175/1‑76 (MQ=255)

                            ggAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttgtt                                 <  1:3718663/76‑1 (MQ=255)

                            ggAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttgtt                                 >  2:5065922/1‑76 (MQ=255)

                             gAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGttt                                <  2:3649503/76‑1 (MQ=255)

                              aaTGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCCACCGGTCGCTTc                               >  2:5756801/1‑76 (MQ=255)

                               aTGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCg                              >  2:1403871/1‑76 (MQ=255)

                               aTGCTATTATCCTGACCAACATATAAGCAGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCg                              >  1:2381616/1‑76 (MQ=255)

                                tGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCgg                             <  2:2854703/76‑1 (MQ=255)

                                 gCTGTTATCCTGACCACCATATAAGCCGAGATTCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGt                            <  1:3203985/76‑1 (MQ=255)

                                 gCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGt                            <  1:4715310/76‑1 (MQ=255)

                                 gCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGt                            >  1:2319197/1‑76 (MQ=255)

                                  cTGTTATCCTGCCCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTg                           <  1:2367505/76‑1 (MQ=255)

                                  cTGTTATCCTGACCACCATATAAGCCGAGAAGCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTg                           <  2:4363215/76‑1 (MQ=255)

                                    tttATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTgta                         <  2:191757/75‑3 (MQ=255)

                                    gTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGaa                         >  2:473736/1‑76 (MQ=255)

                                    gTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGaa                         >  1:359115/1‑76 (MQ=255)

                                     ttATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAAt                        >  1:802862/1‑76 (MQ=255)

                                       ttCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTc                      <  2:1227222/75‑1 (MQ=255)

                                        tCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGACATCAACAGGTTGTTTCGGTGAATTCa                     >  2:5618039/1‑76 (MQ=255)

                                         ccTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAg                    <  1:3016533/76‑1 (MQ=255)

                                          cTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGc                   >  1:529136/1‑76 (MQ=255)

                                          cTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGc                   >  2:1850096/1‑76 (MQ=255)

                                           tGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGGTTCGGTGAATTCAGCg                  >  1:3281358/1‑76 (MQ=255)

                                            gACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCACCGa                 >  2:5185798/1‑76 (MQ=255)

                                             accaccATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttgtt                                 >  2:1568190/1‑59 (MQ=255)

                                             accaccATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGAc                >  1:1750689/1‑76 (MQ=255)

                                               caccaTATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACtt              <  2:3534682/76‑1 (MQ=255)

                                               caccaTATAAGCCGAGAATAGGCGCGCGAAGATCGGGTGCGAGAGCAACACGTGGTTTCGGTGGAGTCAGCGAcct              >  2:1801603/1‑74 (MQ=255)

                                                accaTATAAGCCGAGAATCGGCGCGTTAAGATCGGCTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCTACTTg             >  1:3509805/1‑76 (MQ=255)

                                                 ccaTATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATGAACAGGTTGTTTCGGCGCATGCAGCGTCTTgc            >  1:1892426/1‑75 (MQ=255)

                                                 ccaTATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGt            <  1:1081307/76‑1 (MQ=255)

                                                 ccaTATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGt            >  2:1593024/1‑76 (MQ=255)

                                                  caTATGAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTc           <  2:191439/76‑1 (MQ=255)

                                                  caTATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTc           >  1:1361364/1‑76 (MQ=255)

                                                   atatAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCg          >  2:1215945/1‑76 (MQ=255)

                                                      taAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGccc       >  2:5116892/1‑76 (MQ=255)

                                                      taAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGccc       >  2:2327326/1‑76 (MQ=255)

                                                      taAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGAATTGTCTccc       >  2:4305564/1‑76 (MQ=255)

                                                       aaGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCg      <  1:3930823/76‑1 (MQ=255)

                                                       aaGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCg      <  2:4153188/76‑1 (MQ=255)

                                                       aaGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCg      >  1:3144434/1‑76 (MQ=255)

                                                         gCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTc    <  1:5582927/76‑1 (MQ=255)

                                                         gCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTc    >  2:3005415/1‑76 (MQ=255)

                                                         gCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGCGAATTCAGCGACTCGTCGCCCGTc    >  1:3496089/1‑76 (MQ=255)

                                                          ccGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCa   <  2:4396499/76‑1 (MQ=255)

                                                          ccGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCa   <  2:3143989/76‑1 (MQ=255)

                                                           cGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAg  <  1:404294/76‑1 (MQ=255)

CTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCCG‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAG  >  NC_000913/4013109‑4013242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`