| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,051,170 | +C | intergenic (+172/+279) | hemN → / ← glnG | O2‑independent coproporphyrinogen III oxidase/GlnG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,051,170 | 1 | . | C | 99.1% | 277.0 / ‑3.5 | 114 | intergenic (+172/+279) | hemN/glnG | O2‑independent coproporphyrinogen III oxidase/GlnG |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (63/50); total (64/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
TGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGC > NC_000913/4051094‑4051239 | tGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcg < 1:344628/76‑1 (MQ=255)tGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcg > 2:2102151/1‑76 (MQ=255) gAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgc < 2:2440166/76‑2 (MQ=255) gAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgc < 1:957522/76‑2 (MQ=255) gaaTTGTTGTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgct < 2:5684933/75‑3 (MQ=18) aaaTTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgct < 1:1738880/76‑3 (MQ=255) aaaTTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgct > 2:753531/1‑74 (MQ=255) tGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcg‑cg > 1:3452700/1‑76 (MQ=18) tGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcg‑cg < 1:460886/76‑1 (MQ=18) ggttttGTGGGGCGGATAAGGCGTTTGCGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGt < 2:5323602/74‑1 (MQ=17) tttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCtt < 2:3611446/76‑1 (MQ=18) ttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCtca > 1:3056857/1‑74 (MQ=18) ttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTa < 2:4424094/76‑1 (MQ=18) tGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTAt > 1:3637006/1‑76 (MQ=18) gTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATc > 2:4395508/1‑76 (MQ=18) tAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCa < 2:2256642/76‑1 (MQ=17) aGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAg > 2:5162777/1‑76 (MQ=17) aGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAg > 1:1153518/1‑76 (MQ=17) gCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCCGGTCG‑CGTCCTCTCCGGc > 2:680959/1‑76 (MQ=17) ccGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGcc > 2:5678996/1‑76 (MQ=17) cGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCt < 2:5187965/76‑1 (MQ=17) ggATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTa > 1:790874/1‑76 (MQ=17) ggATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTa < 1:2170883/76‑1 (MQ=17) ggATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGACCCGGCGCCGGGCG‑CGGCTTCTCCGGccca > 2:17670/1‑74 (MQ=2) gATGAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTAc < 2:1057782/76‑1 (MQ=14) gATAGGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTAc < 2:5229387/76‑1 (MQ=14) gATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTAc > 1:12309/1‑76 (MQ=17) gATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTAc > 1:1976523/1‑76 (MQ=17) tAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGCTGCCTGATGCGGCGCTGGGCG‑CGTCTTATCAGGCCCACGa > 1:1242545/1‑76 (MQ=17) tAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGa < 1:2467049/76‑1 (MQ=17) aaGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGAc < 2:4892949/76‑1 (MQ=17) aGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACt > 1:381072/1‑76 (MQ=17) gCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACttt > 2:5241175/1‑76 (MQ=18) gCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACttt < 1:5298661/76‑1 (MQ=18) gTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTAc > 1:1429169/1‑68 (MQ=17) gTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTAc < 2:1429169/68‑1 (MQ=17) gTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTtct > 2:1574908/1‑76 (MQ=18) ttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTcc > 1:4845867/1‑76 (MQ=18) ttACGCAGCATAAGG‑CGTCTGAGCATGATGCCTGATGAGGCGCTGGTCG‑CGTATTATCAGGCCTAAGACTTTCTcc < 2:1153518/76‑1 (MQ=16) tACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCg < 1:1410198/76‑1 (MQ=18) tACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCg < 2:4042498/76‑1 (MQ=18) tACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCg > 1:4161388/1‑76 (MQ=21) aCGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGTGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGc > 2:2705991/1‑76 (MQ=21) aCGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGc > 1:3222830/1‑76 (MQ=21) cgccggATCAGG‑CATCTGAGCATGATGCCTGAGGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCa < 2:3002479/76‑1 (MQ=21) cgccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCa > 2:3212946/1‑76 (MQ=21) gccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCAt > 2:915616/1‑76 (MQ=21) gccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCAt < 1:4542714/76‑1 (MQ=21) gccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCAt > 1:3170321/1‑76 (MQ=21) cccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCAt < 1:1223799/75‑1 (MQ=21) ccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATc > 2:4045179/1‑76 (MQ=25) cgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATcc > 1:5790573/1‑76 (MQ=25) gcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCg > 2:1887012/1‑76 (MQ=25) gcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCg < 1:2894823/76‑1 (MQ=25) cATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg < 1:5678998/76‑1 (MQ=25) cATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg > 2:110897/1‑76 (MQ=25) aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc > 2:5038566/1‑76 (MQ=25) aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGATCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc < 2:4335093/76‑1 (MQ=18) aTCAGG‑CATCAGAGCATGATGCCTGATGCGGCGCTGGGCG‑CGTATTATCAGGCCTACGACTTTCTCCGACTCCGGc > 2:5045465/1‑76 (MQ=18) aTCAGG‑CAGCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc < 2:1867130/76‑1 (MQ=25) tCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCa < 1:2421496/76‑1 (MQ=25) gggcATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATa < 2:183800/73‑1 (MQ=25) aggcATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATa < 2:381072/73‑1 (MQ=25) aggcATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTGTCTCCGCATCCGGCATa > 1:4207133/4‑76 (MQ=25) ggcATCTGAGCTTGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATaa > 1:7522/3‑76 (MQ=25) ggcATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATaa < 2:4643181/74‑1 (MQ=37) ggcATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATaa < 2:2430103/74‑1 (MQ=25) ggcATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATaa < 1:3802799/74‑1 (MQ=25) gcATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATaaa > 2:2693411/2‑76 (MQ=255) cATCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCCACTCCGGCATAaac > 2:1119013/1‑76 (MQ=21) tCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaacaa > 1:980994/1‑76 (MQ=255) tCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaacaa > 2:2912078/1‑76 (MQ=255) tCTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaacaa > 1:4798750/1‑76 (MQ=255) cTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaacaac < 2:3033947/76‑1 (MQ=255) cTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaacaac < 1:3255270/76‑1 (MQ=255) cTGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaacaac > 2:2407061/1‑76 (MQ=255) cTGAGCATCACGCCTGATGCGGCGCTGGGCG‑CGACTTATCAGGCCTACGACAATCTCCGCATCCGGCATAaaccac > 2:3173901/1‑76 (MQ=16) tGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACg > 1:3399480/1‑76 (MQ=255) tGAGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACg < 1:2005735/76‑1 (MQ=255) aGCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAg < 1:753531/76‑1 (MQ=255) gCATGATGCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGTATCCGGCATAAACAACGAGc < 1:5235989/76‑1 (MQ=25) atgatgCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCtt > 1:4197494/1‑76 (MQ=255) atgatgCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCtt > 1:2812979/1‑76 (MQ=255) tgatgCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATACACAACGAGCTTc > 2:3095213/1‑76 (MQ=37) gatgCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCa > 2:4528259/1‑76 (MQ=255) gatgCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCa > 2:267844/1‑76 (MQ=255) gCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCt < 2:1493833/76‑1 (MQ=255) gCCTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCt > 2:1813033/1‑76 (MQ=255) gcTGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTa < 2:3309992/75‑1 (MQ=255) cggATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTaa < 2:3231825/74‑1 (MQ=38) cTGAGGCGGCG‑AGGGCGACGCCTTATCACGCCTGCGGCTCTCGCCGCAGCCGGCATAAACAACGAGCGTCAGCCaa > 2:318237/1‑76 (MQ=9) tGATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTaaa > 2:3431960/1‑76 (MQ=255) gATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAc > 2:4664341/1‑76 (MQ=255) gATGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAc < 1:414984/76‑1 (MQ=255) aTGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc < 2:1747733/51‑1 (MQ=25) aTGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc > 1:1747733/1‑51 (MQ=37) aTGCGGCGCTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACa > 2:761517/1‑76 (MQ=255) aTGCGGCGCGGGTCG‑CGTCTTATCGGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACa < 2:1247476/76‑1 (MQ=38) gcggcgcTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGc < 2:3452700/76‑1 (MQ=255) gcggcgcTGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCACAAACAACGAGCTTCAGCTAACCAGc > 2:5494614/1‑76 (MQ=38) | TGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCG‑CGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGC > NC_000913/4051094‑4051239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |