| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,057,759 | +C | coding (1773/1776 nt) | yihK → | putative GTP‑binding factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,057,758 | 1 | . | C | 98.6% | 367.2 / ‑3.3 | 141 | H591P (CAC→CCC) | yihK | putative GTP‑binding factor |
| Reads supporting (aligned to +/- strand): ref base . (0/2); major base C (48/89); minor base T (0/2); total (48/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
Only 100 of 147 total aligned reads displayed.
AACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑A‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA > NC_000913/4057683‑4057828 | aaCAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑a < 1:3985124/76‑1 (MQ=255) aaGCTCTGGAGTTCATTGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑A‑cct > 2:5017298/1‑74 (MQ=255) aaGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑A‑cct > 1:5819057/1‑74 (MQ=255) aaGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑A‑cct < 2:2127725/76‑3 (MQ=255) tctGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACg > 1:4377241/1‑76 (MQ=255) tctGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACg < 2:3909724/76‑1 (MQ=255) tctGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACg < 2:1547316/76‑1 (MQ=255) tctGGAGTTCATCGATGATGACGAACTGGGAAAAGTGCCTCCGACCTCTATCCGTAGTCGTAAACCT‑C‑ATCTGACg < 1:4060714/76‑1 (MQ=255) ctGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACgg < 2:3515128/76‑1 (MQ=255) tGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGa < 1:2349041/76‑1 (MQ=255) ggAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGaa < 2:2437943/76‑1 (MQ=255) ggAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGC‑C‑ACCTGACGGaa > 2:752179/1‑76 (MQ=255) gAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGaaa < 2:5373952/76‑1 (MQ=255) gAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGaaa > 1:3893628/1‑76 (MQ=255) gAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGaaa < 1:3980503/76‑1 (MQ=255) gAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGaaa < 1:2766573/76‑1 (MQ=255) gTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAAc < 1:356487/76‑1 (MQ=255) ttCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACg < 1:4867609/76‑1 (MQ=255) tCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGa < 2:1932684/76‑1 (MQ=255) cATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGAt < 2:2560373/76‑1 (MQ=255) aTCGATGATTACGAACTGGTAGAAGTTCCTCCGCCCTCTATCCGTCTTCGTAAGCGTCC‑C‑CAGACGGAATACGacc < 2:4280070/76‑3 (MQ=255) aTCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGAtc < 2:4955971/76‑1 (MQ=255) cGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGAtcgt < 1:5595328/76‑1 (MQ=255) gatgatGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGAtcgtc < 1:3775154/76‑1 (MQ=255) gatgatGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGAtcgtc > 2:2625990/1‑76 (MQ=255) gatgatGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGAtcgtc > 1:5054885/1‑76 (MQ=255) atgatgACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGAtcgtcg < 1:5149320/76‑1 (MQ=255) atgatgACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGAtcgtcg > 1:1928447/1‑76 (MQ=255) tgatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTcgc > 1:396849/1‑76 (MQ=255) tgatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTcgc < 2:1257617/76‑1 (MQ=255) gatgaCGCACTGGTAGAAGTGACTCCGACCTCTATCCGTCTTCGTATACGT‑C‑CCCTGACGGAAAACTATCGTggcc < 1:4924098/76‑1 (MQ=255) gatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTcgcc < 2:4997827/76‑1 (MQ=255) gatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTcgcc < 1:4072512/76‑1 (MQ=255) gatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTcgcc < 2:2326104/76‑1 (MQ=255) atgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTcgccg < 1:1654017/76‑1 (MQ=255) tgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGCTCGTcgccgc > 2:5692018/1‑76 (MQ=255) tgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTcgccgc < 1:4829000/76‑1 (MQ=255) tgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTcgccgc < 2:5603445/76‑1 (MQ=255) tgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTcgccgc < 1:4359404/76‑1 (MQ=255) gaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCcgcg > 1:2500897/1‑76 (MQ=255) cGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGcc > 1:5382849/1‑76 (MQ=255) cGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGcc < 1:5280463/76‑1 (MQ=255) gAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCa < 2:2861281/76‑1 (MQ=255) aaCTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCaa > 2:3796905/1‑76 (MQ=255) cTGGTAGAATGGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAAcc < 1:5052594/76‑1 (MQ=255) cTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAAcc < 1:1017254/76‑1 (MQ=255) tGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACcg > 1:3232475/1‑76 (MQ=255) ggTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACcgc < 2:3816436/76‑1 (MQ=255) ggTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACcgc > 1:5744445/1‑76 (MQ=255) ggTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACcgc < 1:5812524/76‑1 (MQ=255) gTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACcgcg < 1:1715964/76‑1 (MQ=255) tAGAAGTGACTCCGACCTCTAGCCGTATTCGTAAACGG‑C‑ACCTGACGGAAAAAGATCGTCGCCGCGCCCACCgcgc > 2:559463/1‑76 (MQ=255) aaGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCTAACCGCGCAcc < 1:1559795/76‑1 (MQ=255) aaGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCAcc < 1:5176619/76‑1 (MQ=255) aaGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCAcc < 2:2572317/76‑1 (MQ=255) aGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCg > 1:657010/1‑76 (MQ=255) gTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGa < 2:3535120/76‑1 (MQ=255) aagACTCCGACCTCTATCCGTATTCGCAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCAGTGCCAACCGCGCACCGa > 1:1226260/3‑76 (MQ=255) tGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGaa < 1:1929404/76‑1 (MQ=255) ggACTCCTACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGaa < 1:277505/75‑1 (MQ=255) gcaCTCGGACCTCTATCCGTATTCGTAAAGGT‑C‑ACCTGTCGGAAAGCGGTCGTCGCCGCGCCAACCGCGCGCCGaa < 2:4418862/74‑1 (MQ=255) gACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGaaa > 2:1192959/1‑76 (MQ=255) gACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGaaa < 2:2582963/76‑1 (MQ=255) aCTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAg < 2:4664253/76‑1 (MQ=255) aCTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAg < 1:5335412/76‑1 (MQ=255) cTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGa < 2:1744735/76‑1 (MQ=255) cTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGa < 1:2996363/76‑1 (MQ=255) cTCCGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGa < 1:4446731/76‑1 (MQ=255) ccGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACg < 1:2427373/76‑1 (MQ=255) ccGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACg > 1:4630325/1‑76 (MQ=255) ccGACCTCTAGCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACg < 2:5143727/76‑1 (MQ=255) cgcccTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGa < 1:2878188/73‑1 (MQ=255) cGACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCTCCGCGCCAACCGCGCACCGAAAGACGa < 2:5462193/76‑1 (MQ=255) gACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAt < 1:1513943/76‑1 (MQ=255) gACCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAt > 1:4413266/1‑76 (MQ=255) aCCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAtt < 1:1076084/76‑1 (MQ=255) aCCTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAtt < 1:3623000/76‑1 (MQ=255) ccTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTa < 2:3273862/76‑1 (MQ=255) ccTCTATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGAGCGTCGCCGCGCCAACCCTGCAACGAAAGGCGATTa > 1:993510/1‑76 (MQ=255) ctctATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTaa > 1:5327558/1‑76 (MQ=255) tctATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAAt < 1:839428/76‑1 (MQ=255) ctATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAAtt < 2:4241940/76‑1 (MQ=255) tATCCGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAAttt < 2:1873938/76‑1 (MQ=255) ccGTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGt > 2:2970497/1‑76 (MQ=255) ccGTATTCGGAAACGG‑CGC‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGt < 2:5200985/76‑1 (MQ=255) gTATTCGTAAACGT‑C‑ACCTGACGGCAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGttt > 2:4219688/1‑76 (MQ=255) gTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGttt > 1:2791583/1‑76 (MQ=255) gTATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGttt > 2:2771129/1‑76 (MQ=255) tATTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCAGCGCCAACCGCGCACCGAAAGACGATTAATTTCGtttt < 1:2326769/76‑1 (MQ=255) aTTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGttttt > 2:2569892/1‑76 (MQ=255) aTTCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGttttt > 1:207399/1‑76 (MQ=255) ttCGGAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGtttttt < 2:3878642/76‑1 (MQ=255) tCGTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTa > 1:2055014/1‑76 (MQ=255) gTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACTGAAAGACGATTAATTTCGTTTTTTAGt < 1:380208/76‑1 (MQ=255) gTAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGt < 2:494498/76‑1 (MQ=255) tAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACcgc < 1:4525087/43‑1 (MQ=37) tAAACGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACcgc > 2:4525086/1‑43 (MQ=37) aCGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATa < 1:5604165/76‑1 (MQ=255) aCGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATa > 2:4773873/1‑76 (MQ=255) cGT‑C‑ACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATaa > 1:3181501/1‑76 (MQ=255) | AACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑C‑A‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA > NC_000913/4057683‑4057828 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |