| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,477,068 | +C | intergenic (‑178/‑242) | b4256 ← / → yjgN | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,477,068 | 1 | . | C | 99.1% | 278.1 / ‑3.6 | 109 | intergenic (‑178/‑242) | b4256/yjgN | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (29/79); total (29/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
CGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGAC‑G‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTT > NC_000913/4476992‑4477136 | cGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑g > 1:281892/1‑76 (MQ=255) ggTAATACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑ATGATTATCCTGCAGCGTCAGGcgac‑gc < 2:3885995/76‑2 (MQ=255) ggTAAGACCGTATTCGGCGGATGCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑gc < 1:2807350/76‑2 (MQ=255) ggTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑gc > 1:788257/1‑75 (MQ=255) ggTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑gc < 1:1022861/76‑2 (MQ=255) ggTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac‑gc < 2:2620152/76‑2 (MQ=255) ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAg < 2:2541291/76‑1 (MQ=255) ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAg > 2:2737224/1‑76 (MQ=255) ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAg < 2:3102997/76‑1 (MQ=255) gTATTCGGCGGATACCTGACGAATGACGCGGGTGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGt < 1:3850921/76‑1 (MQ=255) gTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGt > 2:2472888/1‑76 (MQ=255) gTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTAAAGGt > 1:311865/1‑76 (MQ=255) tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTg < 1:5498250/76‑1 (MQ=255) ttCGGCGGATACCTGCCGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACATGTGAt < 1:1096466/76‑1 (MQ=255) gcggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGAtt < 2:335454/75‑1 (MQ=255) cggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTg < 2:4170384/76‑1 (MQ=255) ggcggATACCTGACGAATGACGCGGGCGATAGC‑ATGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgc < 2:3133612/76‑1 (MQ=255) ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgc > 2:3260897/1‑76 (MQ=255) ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgc > 1:3839462/1‑76 (MQ=255) ggcggAGTCCTGACGAATGACGCGGGCGATAGC‑AGGCTTATCCTGCAGCGTCAGGCGGC‑GCATTTCAGGTGATTgc < 1:313458/76‑1 (MQ=255) aTACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGgcgc < 1:5302537/76‑1 (MQ=255) aTACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGgcgc < 1:1144823/76‑1 (MQ=255) aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGgcgcca < 2:1864855/76‑3 (MQ=255) aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCaa < 1:3171742/76‑1 (MQ=255) aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCaa < 2:4883827/76‑1 (MQ=255) ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAAt < 1:361005/76‑1 (MQ=255) ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAAt < 2:1769659/76‑1 (MQ=255) ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAAt < 1:19286/76‑1 (MQ=255) ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAAt < 1:262312/76‑1 (MQ=255) cTGACGAGTGACGCGGGCGATAG‑GAGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATg < 2:3052689/76‑1 (MQ=255) cTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGAt > 2:2335051/1‑64 (MQ=255) cTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGAt < 1:2335051/64‑1 (MQ=255) cTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATg < 2:2993415/76‑1 (MQ=255) tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGGCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAAAGt > 1:4909100/1‑76 (MQ=255) gACGAATGACTCGTGCGTTAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGtt < 2:3651079/76‑1 (MQ=255) gACGAATGACGCGGGCGATAGC‑ATGATTATCCTGCGGCGTCAGGCGAC‑GCATTGCAGGTGATTGCGGCGCAATGtt < 1:4607719/76‑1 (MQ=255) gACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgc > 2:5801250/1‑65 (MQ=255) gACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTgc < 1:5801251/65‑1 (MQ=255) gACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGtt < 1:5138595/76‑1 (MQ=255) ggAAGGACGGGGGCGATAG‑GAGGATTATCCTACAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAt < 2:5129799/75‑1 (MQ=255) gAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGCC‑GCATTACAGGTGATTGCGGCGCAATGTTAtt < 2:1076214/76‑1 (MQ=255) gAATGACGCGGGCGATAGC‑AGGAGTATCCTGCAGCGTCAGGCGCC‑GCATTGCTGGTGATTGTGGCGCGCTGTTAtt < 2:1122822/76‑1 (MQ=255) gAATGACGCAGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAtt < 2:1958401/76‑1 (MQ=255) aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTAttt > 1:1117532/1‑75 (MQ=255) aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTc < 2:2282166/76‑1 (MQ=255) aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTc < 1:1900909/76‑1 (MQ=255) aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTc < 2:3852652/76‑1 (MQ=255) aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTc < 2:281892/76‑1 (MQ=255) aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTc < 1:837004/76‑1 (MQ=255) aTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCa > 1:1452537/1‑76 (MQ=255) tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCAt < 1:664469/76‑1 (MQ=255) tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCAt < 1:5590518/76‑1 (MQ=255) gACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATa < 1:3286893/76‑1 (MQ=255) aCGCGGGCGATAGC‑AGGATTATCCTGCATCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAg < 2:3649993/76‑1 (MQ=255) cgcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt < 1:5806381/76‑1 (MQ=255) cgcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt < 1:3951260/76‑1 (MQ=255) cgcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt < 2:1414661/76‑1 (MQ=255) cgcgGGCGATAGC‑AGGATGATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt < 1:2640525/76‑1 (MQ=255) cgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGttt < 1:1981063/76‑1 (MQ=255) cgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCCGCGCAATGTTATTCATAGttt > 2:856091/1‑76 (MQ=255) ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa < 2:4423599/76‑1 (MQ=255) ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa < 1:3629538/76‑1 (MQ=255) ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa < 1:327221/76‑1 (MQ=255) gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAc < 1:250940/76‑1 (MQ=255) cGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAcc < 1:189223/76‑1 (MQ=255) cGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAcc < 1:2635252/76‑1 (MQ=255) cGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAcc < 2:2100745/76‑1 (MQ=255) gcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaata < 1:395752/74‑1 (MQ=255) gcaGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaata < 2:2660022/74‑1 (MQ=255) caGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataa < 2:1442837/75‑1 (MQ=255) aGGATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaa < 2:5128745/76‑1 (MQ=255) gATTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaa < 2:4182793/76‑1 (MQ=255) aTTATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaat < 2:308109/76‑1 (MQ=255) ttATCCTGCTGCGTCAGGTGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaata < 2:3626218/76‑1 (MQ=255) ttATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaata < 1:2830837/76‑1 (MQ=255) ttATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaata < 1:3921382/76‑1 (MQ=255) tATCCTGCAGCTTCAGGCAAGTG‑GTTACAGGTGCTTGCGTCGCAATGTTCTTCATAGTTTAACCcaataacaataa < 2:2476195/76‑1 (MQ=255) tATCCTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaataa > 1:2104232/1‑76 (MQ=255) ttCCTGCGGCGTCGGGCGAC‑GCATTGCAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaataaa < 2:169703/75‑1 (MQ=255) ccTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAga > 2:4643879/1‑75 (MQ=255) ccTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGt < 2:3991088/76‑1 (MQ=255) cTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa < 1:31259/76‑1 (MQ=255) cTGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa < 1:222387/76‑1 (MQ=255) tGCAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTaa > 1:1468874/1‑76 (MQ=255) cAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAtt > 1:164411/1‑76 (MQ=255) cAGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAtt > 2:5658202/1‑76 (MQ=255) aGCGTCAGTCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAtt > 2:2704974/1‑75 (MQ=255) aGCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta < 1:4217236/76‑1 (MQ=255) gCGTCAGGCTAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATtat < 1:3513697/76‑1 (MQ=255) gCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATtat > 2:1114071/1‑76 (MQ=255) gCGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGGTATTCACAGTTTAACCCAATCAAAATAAAGTAATtat > 2:1123394/1‑76 (MQ=255) cGTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGCAATtata > 2:1815517/1‑76 (MQ=255) gTCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAc > 2:3678857/1‑76 (MQ=255) tCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa > 2:3716982/1‑76 (MQ=255) tCAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa > 2:1015647/1‑76 (MQ=255) cAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAt < 1:1521285/76‑1 (MQ=255) cAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAt < 2:2004011/76‑1 (MQ=255) cAGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAt < 1:323829/76‑1 (MQ=255) aGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAtt > 2:4267249/1‑76 (MQ=255) aGGCGAC‑GCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAtt < 2:2995164/76‑1 (MQ=255) | CGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGAC‑G‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTT > NC_000913/4476992‑4477136 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |