| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,501,186 | (T)6→7 | intergenic (‑187/‑440) | yi41 ← / → b4279 | IS4 hypothetical protein/putative transport protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,501,180 | 1 | . | T | 99.1% | 345.4 / ‑3.3 | 107 | intergenic (‑181/‑446) | yi41/b4279 | IS4 hypothetical protein/putative transport protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (51/55); total (52/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
ACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑TTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCT > NC_000913/4501105‑4501250 | aCAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTg < 1:4802928/76‑1 (MQ=255) aaCTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑ttt > 2:4946826/1‑76 (MQ=255) aCTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑ttt‑t > 1:3204292/1‑76 (MQ=255) tATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑ttt‑ttt < 2:3641912/76‑1 (MQ=255) aTCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑ttt‑ttgt < 1:194198/76‑3 (MQ=255) tCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑ttt‑ttttg < 2:663474/76‑3 (MQ=255) tCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑ttt‑ttttg < 2:3258748/76‑3 (MQ=255) cAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑ttt‑ttttga > 1:1530926/1‑73 (MQ=255) cAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑ttt‑ttttga < 1:4327371/76‑4 (MQ=255) cTACAAAAATATGCTCAATTTGTGGCATAAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCt > 1:1955657/1‑76 (MQ=255) tACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTg < 2:1946600/76‑1 (MQ=255) aCAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTgg > 1:3044571/1‑76 (MQ=255) cAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGt < 1:1917221/76‑1 (MQ=255) cAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGt > 2:4943372/1‑76 (MQ=255) cAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGt > 2:5210253/1‑76 (MQ=255) aaaaTATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGtat < 1:2207327/76‑1 (MQ=255) aaaTATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGtata > 2:3282211/1‑76 (MQ=255) aaaTATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGtata < 1:4450846/76‑1 (MQ=255) atatGCTCAATTTGTGACATCAGTAACAAAACGCGTTGTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAAc < 2:4757317/76‑1 (MQ=255) tatGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACa < 1:5189635/76‑1 (MQ=255) tatGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACa < 1:978817/76‑1 (MQ=255) tatGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACa > 2:2076417/1‑76 (MQ=255) tatGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACa < 1:1702519/76‑1 (MQ=255) aatGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGGTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACa < 1:5219182/75‑1 (MQ=255) atGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAg > 1:2739857/1‑76 (MQ=255) atGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAg > 1:1571866/1‑76 (MQ=255) atGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAg < 1:2834073/76‑1 (MQ=255) tCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGtat < 1:4589022/76‑1 (MQ=255) cAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGtata < 2:636464/76‑1 (MQ=255) aaTTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATaa < 2:2094295/76‑1 (MQ=255) aTTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATaaa < 1:2521763/76‑1 (MQ=255) aTTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATaaa < 1:3512836/76‑1 (MQ=255) tttGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAg < 2:4745765/76‑1 (MQ=255) ttGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAgg > 1:4615052/1‑76 (MQ=255) ttGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAgg > 1:93658/1‑76 (MQ=255) tgtgACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGt < 2:2663757/76‑1 (MQ=255) gtgACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGta < 2:4810456/76‑1 (MQ=255) tgACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGtat > 1:4042652/1‑76 (MQ=255) tgACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGtat > 2:5583261/1‑76 (MQ=255) tgACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGtat < 2:1818677/76‑1 (MQ=255) gACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACGGGTATAAAGGtata < 1:1468498/76‑1 (MQ=37) gACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGtata > 2:4570465/1‑76 (MQ=255) gACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGtata < 2:1610990/76‑1 (MQ=255) gACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGtata > 1:3144395/1‑76 (MQ=255) cATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACa < 2:5746372/76‑1 (MQ=255) aTCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATCCAg < 2:1548892/76‑1 (MQ=37) aTCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAg > 1:247624/1‑76 (MQ=255) aTCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAg > 1:2691001/1‑76 (MQ=255) aTCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAg > 1:723237/1‑76 (MQ=255) tCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGa > 2:1369492/1‑76 (MQ=255) tCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGa < 1:5774047/76‑1 (MQ=255) aGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGaaa > 2:4494524/1‑76 (MQ=255) aGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGaaa > 1:2284653/1‑76 (MQ=255) aGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGaaa > 2:3514460/1‑76 (MQ=255) gTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGaaaa > 2:3140258/1‑76 (MQ=255) gTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGaaaa > 1:2324556/1‑76 (MQ=255) gTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGaaaa < 2:3441300/76‑1 (MQ=255) gTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGaaaa > 1:3430255/1‑76 (MQ=255) gTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGaaaa < 1:3515745/76‑1 (MQ=255) aCAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCa > 1:3837161/1‑76 (MQ=255) aCAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCa > 1:2507986/1‑76 (MQ=255) cAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCaa < 2:2739857/76‑1 (MQ=255) cAAAACGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCaa < 2:2066158/76‑1 (MQ=255) aaaaCGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCaaa > 2:5367600/1‑76 (MQ=255) aaaaCGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCaaa > 2:1397450/1‑76 (MQ=255) aaCGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGa > 2:511162/1‑76 (MQ=255) aaCGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGa < 1:744684/76‑1 (MQ=255) aCGCGTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGaa > 2:4066571/1‑76 (MQ=255) cgcgTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGaaa < 1:3540661/76‑1 (MQ=255) cgcgTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGaaa < 1:3511324/76‑1 (MQ=255) cgcgTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGaaa < 1:5367601/76‑1 (MQ=255) gcgTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAAt < 1:3787447/76‑1 (MQ=255) cgTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATAGAGAAAAGCAAAGAAATa < 1:271241/76‑1 (MQ=37) cgTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATa > 1:5610155/1‑76 (MQ=255) gTTTTGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATAc < 1:3368648/76‑1 (MQ=255) ttttGTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACt < 1:1369492/76‑1 (MQ=255) gTTATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGGAAAGCAAAGAAATACTGCaa > 1:4351685/1‑76 (MQ=37) ttATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGACATACTGCaac > 2:4641313/1‑75 (MQ=37) ttATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCaaa > 2:4634164/1‑76 (MQ=255) ttATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCaaa < 1:3811392/76‑1 (MQ=255) ttATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCaaa < 2:4110577/76‑1 (MQ=255) ttATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCaaa < 1:739944/76‑1 (MQ=255) ttATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCaaa < 2:2562238/76‑1 (MQ=255) tATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACGGAAAAGCAAAGAAATACTGc < 2:2709518/72‑1 (MQ=37) tATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACGGAAAAGCAAAGAAATACTGc > 1:2709518/1‑72 (MQ=37) tATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAg > 1:5833542/1‑76 (MQ=255) tATGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAg < 2:4828381/76‑1 (MQ=255) aTGTGGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAgg > 2:1894329/1‑76 (MQ=255) gtgGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaa < 1:570852/76‑1 (MQ=255) tgGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaaa > 1:5764744/1‑76 (MQ=255) tgGATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaaa > 2:4730161/1‑76 (MQ=255) ggATTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaaaa < 2:93205/76‑1 (MQ=255) aTTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACa < 1:4494525/76‑1 (MQ=255) aTTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACa > 1:2156661/1‑76 (MQ=255) aTTGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACa > 1:1372731/1‑76 (MQ=255) ttGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAg < 1:4570466/76‑1 (MQ=255) tGCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGc < 1:297066/76‑1 (MQ=255) tGCTTG‑TTTGTTTGATCGGGAATAACAGGTATAAAGGGATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGc > 1:4215093/1‑76 (MQ=255) gCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCt > 2:1272761/1‑76 (MQ=255) gCTTGTTTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCt < 1:4943373/76‑1 (MQ=255) | ACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑TTT‑TTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCT > NC_000913/4501105‑4501250 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |