BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,008,480	+A	coding (637/654 nt)	yedK →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,008,476	1	.	A	94.2%	310.8 / 11.5	104	coding (633/654 nt)	yedK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (3/3); new base A (45/52); total (48/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑AAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTC  >  NC_000913/2008401‑2008546

aGAAATCGCGGCCAGTGGCTGGGTTCCAGCAAACCAGTTTTCCTGGCCCCCGGTATCGCGGCCGGGGGGGGATG‑tt                                                                            >  1:1800586/1‑76 (MQ=255)

  aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑aa                                                                         <  1:5607005/76‑1 (MQ=255)

    aTCGCGGCCAGTGGCGGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑aaa‑a                                                                      <  2:140879/76‑1 (MQ=255)

     tCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑aaa‑aa                                                                     <  2:1852215/76‑2 (MQ=255)

      cgcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑aaa‑aac                                                                    <  2:3484273/76‑3 (MQ=255)

       gcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑aaa‑aacc                                                                   <  1:2214902/76‑4 (MQ=255)

       gcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCCGGCACCCCGTAGCGCGCGCGGGGGGTAATG‑TT‑aaa‑aacc                                                                   >  2:3171576/1‑73 (MQ=255)

       gcgGCCAGTGGCTGGGTTCCCGCAAACCAGTTCTCCTGGCCCCCCGGATCCCCCGCGGGGGGGAATG‑TT‑aaa‑aaac                                                                   >  2:435850/1‑73 (MQ=255)

       gcgGCCAGTGGCTGGGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGGGGGGGATG‑TT‑aaa‑aaac                                                                   >  1:4593984/1‑73 (MQ=255)

         ggCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGGGGGGAATGTTT‑AAA‑A‑C‑CAg                                                                >  1:4257759/1‑76 (MQ=255)

          gCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAgg                                                               <  2:4472848/76‑1 (MQ=255)

          gCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAgg                                                               <  2:1377466/76‑1 (MQ=255)

           ccAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAggg                                                              <  2:2272127/76‑1 (MQ=255)

           ccAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAggg                                                              <  2:3058466/76‑1 (MQ=255)

            cAGTGGCTGTGTTCCCGCAAACCAGTTCCCCTGGCACCCGGTATCGCGCCCGGGGGGGGACG‑TT‑AAA‑A‑CACAgggg                                                             >  2:5721530/1‑75 (MQ=255)

            cAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGgc                                                             <  1:5570010/76‑1 (MQ=255)

            cAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGgc                                                             <  2:2454872/76‑1 (MQ=255)

            cAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGGGGGTAATG‑TTAAAA‑A‑C‑CAGGgc                                                             >  2:3163881/1‑76 (MQ=255)

             aGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGgcg                                                            <  1:3070199/76‑1 (MQ=255)

              gTGGCTGTGTTCTGGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGgcgc                                                           <  2:4194407/76‑1 (MQ=255)

               tGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGcgcg                                                          >  1:2829541/1‑76 (MQ=255)

                 gCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGa                                                        <  1:3755344/76‑1 (MQ=255)

                   tgtgTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGt                                                      >  1:928203/1‑76 (MQ=255)

                   tgtgTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGt                                                      <  1:5234873/76‑1 (MQ=255)

                     tgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTa                                                    >  1:5255132/1‑76 (MQ=255)

                      gtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTaa                                                   <  2:2627018/76‑1 (MQ=255)

                       ttCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAAt                                                  >  2:3028202/1‑76 (MQ=255)

                       ttCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAAt                                                  >  2:2366325/1‑76 (MQ=255)

                        tCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAAtt                                                 >  1:5368332/1‑76 (MQ=255)

                         ccAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTc                                                >  2:3817612/1‑76 (MQ=255)

                         ccAGCAAACCAGTTCCCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTc                                                >  2:1526793/1‑76 (MQ=255)

                          cAGCAAACCAGTTCTCCTGGCACCCAGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCa                                               >  2:4263665/1‑76 (MQ=255)

                           aGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCaa                                              >  2:3245852/1‑76 (MQ=255)

                           aGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCaa                                              <  1:2059922/76‑1 (MQ=255)

                            acAAACCAGTTCTCCGGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAAc                                             <  2:505770/75‑1 (MQ=255)

                             cAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAAcc                                            <  1:5166786/76‑1 (MQ=255)

                              aaaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCt                                           <  1:3857284/76‑1 (MQ=255)

                              aaaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCt                                           <  1:2169390/76‑1 (MQ=255)

                              aaaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCt                                           <  2:2588555/76‑1 (MQ=255)

                              aaaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGCGGGCAATG‑TTAAAA‑A‑C‑CAGGGCGAGGAGTTAATTCAACCt                                           >  1:866304/1‑76 (MQ=255)

                               aaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTg                                          >  2:4245467/1‑76 (MQ=255)

                               aaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTg                                          >  1:2346637/1‑76 (MQ=255)

                                  cAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGttt                                       <  2:1693840/76‑1 (MQ=255)

                                  cAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGttt                                       >  2:2616745/1‑76 (MQ=255)

                                    gTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGa                                     <  2:3648526/76‑1 (MQ=255)

                                     ttCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGCTA‑AAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGAt                                    >  1:4914468/1‑76 (MQ=255)

                                     ttCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGAt                                    >  1:5722173/1‑76 (MQ=255)

                                     ttCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGAt                                    <  1:1814826/76‑1 (MQ=255)

                                       ctcCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                  <  2:4038945/76‑1 (MQ=255)

                                       ctcCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                  >  2:519712/1‑76 (MQ=255)

                                        tcCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAg                                 <  1:4929809/76‑1 (MQ=255)

                                        tcCGGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAg                                 <  2:4923794/76‑1 (MQ=255)

                                          cTGGCACGCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGcc                               <  2:900684/76‑1 (MQ=255)

                                           tGGCACCGGTTATCGTGCGCAGTGAGTAATG‑TTCAAT‑C‑C‑CAGGGCTCGGAGTTAATTCAACCTGTTTGATAATCCt                              <  1:5544034/76‑1 (MQ=255)

                                           tGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCt                              >  1:1164829/1‑76 (MQ=255)

                                           tGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCt                              >  2:1677029/1‑76 (MQ=255)

                                           tGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCt                              >  1:3807685/1‑76 (MQ=255)

                                             gCACCCGGTGTCGCGGGCGGTGGGTAATG‑TT‑AAA‑AGC‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTgg                            <  2:1869566/76‑1 (MQ=255)

                                             gCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATCAGCCTgg                            >  2:2834640/1‑76 (MQ=255)

                                             gCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTgg                            >  2:5788554/1‑76 (MQ=255)

                                             gCACCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTgg                            <  2:1254250/76‑1 (MQ=255)

                                               aCCCGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGgag                          <  2:1434208/76‑1 (MQ=255)

                                                 ccGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGat                        <  1:2751040/76‑1 (MQ=255)

                                                 ccGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGat                        >  2:4093066/1‑76 (MQ=255)

                                                 ccGGTATCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGat                        >  1:1774630/1‑76 (MQ=255)

                                                 ccGGTATCGCGCGCGGTGGGTAATG‑TT‑AAA‑AGC‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGat                        <  1:1739013/76‑1 (MQ=255)

                                                  cGGTAGCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGatt                       <  2:852314/76‑1 (MQ=255)

                                                     tATCGCGCGCGGTGGGCAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGattatt                    >  2:3749829/1‑76 (MQ=255)

                                                      aTCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGGTAATTCAACCTGTTTGATAAGCCTGGAGATTAGTg                   >  1:1745594/1‑76 (MQ=255)

                                                       tCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGa                  <  1:1990614/76‑1 (MQ=255)

                                                       tCGCGCGCGGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGa                  <  1:2019203/76‑1 (MQ=255)

                                                       gcgcgcgcgGGGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGa                  <  2:5656183/75‑1 (MQ=255)

                                                        cgcgcgcgGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGAt                 <  2:5208089/76‑1 (MQ=255)

                                                         gcgcgcgGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATc                >  1:4723915/1‑76 (MQ=255)

                                                          cgcgcgGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATca               <  2:5408528/76‑1 (MQ=255)

                                                           gcgcgGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                  <  1:146306/56‑1 (MQ=255)

                                                           gcgcgGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaa              <  2:1993956/76‑1 (MQ=255)

                                                            cgcgGTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaac             >  2:3755393/1‑76 (MQ=255)

                                                            cgcgGTGGGCAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaac             >  1:636917/1‑76 (MQ=255)

                                                             gcgGTGGGTAATG‑TT‑AAAGG‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGTTTATTGATcaaca            <  2:866304/76‑1 (MQ=255)

                                                               ggTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAAt          <  1:2638686/76‑1 (MQ=255)

                                                               ggTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAAt          >  1:365557/1‑76 (MQ=255)

                                                               ggTGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAAt          <  1:3269041/76‑1 (MQ=255)

                                                                 tGGGTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGGTAAGCCTGGAGATTATTGATCAACAATAc        <  2:1575001/76‑1 (MQ=255)

                                                                  gggTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACt       >  1:544853/1‑76 (MQ=255)

                                                                  gggTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACt       <  2:636917/76‑1 (MQ=255)

                                                                   ggTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTg      <  2:3477754/76‑1 (MQ=255)

                                                                   ggTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTg      <  2:4869772/76‑1 (MQ=255)

                                                                   ggTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTg      >  1:3895666/1‑76 (MQ=255)

                                                                   ggTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTg      <  2:389724/76‑1 (MQ=255)

                                                                    gTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGc     <  1:2443682/76‑1 (MQ=255)

                                                                    gTAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGc     <  1:2493437/76‑1 (MQ=255)

                                                                     tAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCg    >  1:3975964/1‑76 (MQ=255)

                                                                     tAATG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCg    >  2:2520954/1‑76 (MQ=255)

                                                                     gaaTG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCg    <  2:247286/75‑1 (MQ=255)

                                                                      aaTG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGt   >  1:1745717/1‑76 (MQ=255)

                                                                      aaTG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGt   <  2:5368331/76‑1 (MQ=255)

                                                                      aaTG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGt   >  2:1351465/1‑76 (MQ=255)

                                                                       aTG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTc  >  2:2954285/1‑76 (MQ=255)

                                                                       aTG‑TTAAAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTc  <  1:3028202/76‑1 (MQ=255)

AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATG‑TT‑AAA‑A‑C‑CAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTC  >  NC_000913/2008401‑2008546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`