| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,005,399 | +C | coding (1044/1092 nt) | ygeW → | putative carbamoyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,005,398 | 1 | . | C | 98.7% | 409.3 / ‑1.3 | 154 | coding (1043/1092 nt) | ygeW | putative carbamoyl transferase |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (68/83); total (71/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 165 total aligned reads displayed.
AAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑T‑CTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGA > NC_000913/3005323‑3005468 | aaGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGACAGCTGGAAGCCTTATATAAGCGCCGCgatga‑t > 1:5064638/1‑76 (MQ=255) aTTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTcc < 2:4694821/76‑1 (MQ=255) ttCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTccc < 2:1244360/76‑1 (MQ=255) ttCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTccc < 2:1149090/76‑1 (MQ=255) tCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCg > 1:5835186/1‑76 (MQ=255) tCGAAAAATACCGTATCGCGACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCg < 1:1651883/76‑1 (MQ=255) cGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGt > 2:473992/1‑76 (MQ=255) gAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTa > 2:2567204/1‑76 (MQ=255) aaaaaTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTaa > 1:492679/1‑76 (MQ=255) aaaaaTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTaa < 2:4047153/76‑1 (MQ=255) aaaTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTaaa > 1:545925/1‑75 (MQ=255) aaaTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAAt < 2:3612148/76‑1 (MQ=255) aTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATAc > 2:2858725/1‑76 (MQ=255) aTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATAc > 1:4308791/1‑76 (MQ=255) aTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATAc < 1:895438/76‑1 (MQ=255) aTACCGTATCGCTACCTACAAAGAAGCCAGCTGCAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATAc > 1:3621996/1‑76 (MQ=255) tACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTACATACg > 2:3147689/1‑76 (MQ=255) tACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACg < 1:47307/76‑1 (MQ=255) aCCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGc < 2:2317831/76‑1 (MQ=255) cGTATCGCGCCCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCa < 2:1543222/76‑1 (MQ=255) gTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCaa < 1:1998329/76‑1 (MQ=255) gTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCaa < 1:177485/76‑1 (MQ=255) gTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCaa > 1:4991252/1‑76 (MQ=255) aTCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAAc > 2:86379/1‑76 (MQ=255) aTCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAAc < 1:3488361/76‑1 (MQ=255) aTCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAAc > 1:5568367/1‑76 (MQ=255) aTCGCTACCTACAAAGAAGCCAGCTGGAAGACTTATATCAGCGCCGCGATGA‑TCCTGTTCCGCACATACGACAAAc > 2:304438/1‑76 (MQ=255) aTCGCTACCTACAAAGAAGCCAACTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTCTCCCGCAAATACGCCAAAc > 2:691875/1‑76 (MQ=255) tCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAAcc < 1:5231456/76‑1 (MQ=255) tCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAAcc < 2:4429583/76‑1 (MQ=255) tCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAAcc > 2:3996997/1‑76 (MQ=255) tCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCAGCGATGA‑TCCTGTCCCGTAAATACTCTAAAcc > 1:162386/1‑76 (MQ=255) tCGCTAAATACAAACAAGCCAGCGGGAAGCCGTATATCATCGCCGCGAGGAGC‑CTGGCCAGCAAACACGCCAAAcc > 2:5426690/1‑76 (MQ=255) cGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCACACCa > 1:4402712/1‑76 (MQ=255) cGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCa < 2:4696254/76‑1 (MQ=255) cGCTACCTAATACTAAGCCAGCTCGAAGCCTTATATCATCGGCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCa < 1:3411833/76‑1 (MQ=255) gCTACCTACAAAGCAGCCAGCTGGACGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAACTACGCCACAcccg > 2:1499847/1‑74 (MQ=255) gCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAACTACGCCAAACCAg > 2:2552178/1‑76 (MQ=255) gCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAg > 2:2512924/1‑76 (MQ=255) gCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAg > 1:1906546/1‑76 (MQ=255) tacctacAAAGAAGCTAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAggg > 2:2395193/1‑75 (MQ=255) tacctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGt < 2:4648614/76‑1 (MQ=255) taccgacAAAGAGGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGt < 1:1801857/76‑1 (MQ=255) cctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGc > 1:3412525/1‑76 (MQ=255) ctacAACGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAACTACGCCAAACCCGGTGCa > 1:4004575/1‑76 (MQ=255) ctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCa > 1:3533314/1‑76 (MQ=255) tacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCAc < 1:5288813/76‑1 (MQ=255) acAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACt > 2:2657723/1‑76 (MQ=255) acAAAGAAGCCAGCTGGAAGCCTTATAGCATCGCCGCGATGA‑ACCTGTCCCGTAAATACTCCAAACCAGCTGCAcc > 2:5274577/1‑75 (MQ=255) cAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTg < 1:2778563/76‑1 (MQ=255) cAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTg < 1:3304412/76‑1 (MQ=255) cAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTg < 2:2251395/76‑1 (MQ=255) aaaGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGc < 1:4126736/76‑1 (MQ=255) aggaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGTTGCACTGCt < 1:2932653/74‑1 (MQ=255) aagaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCt < 2:5483849/76‑1 (MQ=255) agaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTc < 2:1757213/76‑1 (MQ=255) agaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTc < 1:1436095/76‑1 (MQ=255) agaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTc < 2:3280145/76‑1 (MQ=255) agaagCCAGCGGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTc < 2:2182864/76‑1 (MQ=255) gaagCCAGCTGGAGTCCTTATATCATCGCTGCGATGA‑TCCTGTCCCGTAAATACGCCACACCAGGTGCGCTGCTca < 2:4446279/76‑2 (MQ=255) gaagCCAGCTGGAAGCCTTCTATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCg > 1:4815050/1‑76 (MQ=255) gaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCg > 2:2796961/1‑76 (MQ=255) gaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCg > 1:4766219/1‑76 (MQ=255) aagCCTGCTGGAGTCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGa < 1:1098711/76‑1 (MQ=255) aagCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGa < 1:3058462/76‑1 (MQ=255) gCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGc > 1:1726649/1‑76 (MQ=255) gCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGc < 1:3157751/76‑1 (MQ=255) ccAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCa > 1:460910/1‑76 (MQ=255) aGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAAc > 1:2568240/1‑76 (MQ=255) gCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAAct > 1:598613/1‑76 (MQ=255) cTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActg < 2:712150/76‑1 (MQ=255) cTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActg < 1:4347644/76‑1 (MQ=255) tGGAAGCCTTATATCATCGCCGCGATGA‑TACTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAAccgc > 2:5787195/1‑76 (MQ=255) aaGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGa > 2:1310155/1‑76 (MQ=255) aGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGaa > 1:2870516/1‑76 (MQ=255) gCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGaaa < 1:1052889/76‑1 (MQ=255) gCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGaaa < 1:86379/76‑1 (MQ=255) cgtataTCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAaaga < 2:3663655/74‑1 (MQ=255) cTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAACCCAGGTGCTCTGCTCGAGCAAGTGCTGAaaga < 2:5064637/76‑1 (MQ=255) cTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAaaga > 2:240942/1‑76 (MQ=255) cTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAaaga > 1:2958660/1‑76 (MQ=255) ttATATCATCGCCTCGACGACG‑CGGTACCGCAAATACGCCAAACCACGAAAACTGCGCCAGCAACTGCTGgaagaa > 2:3225493/1‑76 (MQ=255) ttATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAaagaa > 2:1871281/1‑76 (MQ=255) atatCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgc < 1:2200854/76‑1 (MQ=255) atatCACCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAGCCAGGTGCACTGCTCGAGCAACTTCTGAAAGAAgc < 1:657347/76‑1 (MQ=255) tatCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgcg > 2:2002740/1‑76 (MQ=255) tatCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgcg > 1:5426412/1‑76 (MQ=255) tcatcGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCa > 2:1909752/1‑76 (MQ=255) tcatcGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCa > 2:1966691/1‑76 (MQ=255) cagcgccgcGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGATCAGCTGCTGAAAGAAGCGCaa < 2:1610777/73‑1 (MQ=255) tcGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGa > 1:3927803/1‑76 (MQ=255) gccgcGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAc < 1:5686946/76‑1 (MQ=255) gccgcGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAc > 1:473892/1‑76 (MQ=255) ccgcGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAcg < 2:436366/76‑1 (MQ=255) cgcgATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAcgc < 1:1310155/76‑1 (MQ=255) cgcgATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAcgc < 1:4946381/76‑1 (MQ=255) cgATGA‑TCCTGTCCCGTAAATTCGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGt < 2:5568365/76‑1 (MQ=255) gatga‑tcCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTg < 1:2063604/76‑1 (MQ=255) atga‑tcCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGa < 2:1580229/76‑1 (MQ=255) atga‑tcCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGa > 1:1675688/1‑76 (MQ=255) | AAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑T‑CTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGA > NC_000913/3005323‑3005468 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |