| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,123,585 | +G | coding (953/2286 nt) | glcF ← | glycolate oxidase iron‑sulfur subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,123,582 | 1 | . | G | 93.8% | 196.3 / 9.1 | 80 | coding (956/2286 nt) | glcF | glycolate oxidase iron‑sulfur subunit |
| Reads supporting (aligned to +/- strand): ref base . (3/2); new base G (46/29); total (49/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
ACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑GGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGA > NC_000913/3123507‑3123651 | agcGGGGTTAAACAGCCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑c < 1:2275171/74‑1 (MQ=255)aCCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑c < 1:1551985/76‑1 (MQ=255) cGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑gg < 1:1778973/76‑1 (MQ=255) ggggTTAAAGCGGCCGCAGGGGTCGGGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑ggg < 2:497099/76‑1 (MQ=255) ggggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑ggg < 2:4150715/76‑1 (MQ=255) ggggTAAACCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑ggg < 1:2073012/76‑1 (MQ=255) gggTTAGCCACGCCGCAAGGGTCGGGCGGCTGTTTAAGCTGGTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑gggg < 2:2347474/76‑2 (MQ=255) gggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑gggg > 2:2508719/1‑75 (MQ=255) gggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑gg > 1:5122824/1‑74 (MQ=255) gggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑gg < 2:5122823/74‑1 (MQ=255) gggTGAAACTCGCCGCAAGGGGCGGGCTGCGGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑gggg < 2:5390662/76‑2 (MQ=255) ggTTAAACACGCGGCAGGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑ggggc < 2:2825305/76‑3 (MQ=255) ggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑ggggc > 1:2736822/1‑74 (MQ=255) aaCCCGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAG‑GGAGCCGATA‑G‑CGGGGC‑AAAGc < 2:1108827/76‑1 (MQ=255) acacGCCGCAGGGGTCGGGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGcc < 2:3806072/76‑1 (MQ=255) acacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGcc > 1:4129203/1‑76 (MQ=255) acacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGcc > 2:4495112/1‑76 (MQ=255) cacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGccc > 2:1549088/1‑75 (MQ=255) acGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGccac > 1:86243/1‑76 (MQ=255) gccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGccacca > 1:5399183/1‑76 (MQ=255) gccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGACA‑G‑CGGGGC‑CAAGccaccc > 2:5512904/1‑75 (MQ=255) cgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCCtc > 2:2888849/1‑76 (MQ=255) cgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCAtc > 1:3018425/1‑76 (MQ=255) cgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCAtc < 1:5741049/76‑1 (MQ=255) cgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCAtc > 1:5616235/1‑76 (MQ=255) gcAGGGGTCGAGCGGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGGCACCAtct < 2:1178292/76‑1 (MQ=255) caggggTGGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCAtctc < 1:5061755/73‑1 (MQ=255) cAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCCtctc > 2:1523967/1‑76 (MQ=255) aaGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTcc > 2:1862968/1‑76 (MQ=255) aGGGTGGAGTTGCTGTTTAGGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCg < 1:4572845/76‑1 (MQ=255) aGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCAATA‑G‑CGGGGC‑AAAGCCACCATCTCCg > 1:1933455/1‑76 (MQ=255) gggTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCgg < 1:5095661/76‑1 (MQ=255) tCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑ACAGCCACCATCTCCCGccc > 2:705052/1‑74 (MQ=255) tCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGccc > 2:5807498/1‑74 (MQ=255) cGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑CAAGCCACCATCTCCGGCACt > 1:2961876/1‑76 (MQ=255) aGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTaa > 1:5190075/1‑76 (MQ=255) gctgctGTTTAGGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTaaa < 1:629937/76‑1 (MQ=255) gtgctgTTTAAGGGGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAg < 2:5274966/75‑1 (MQ=255) ctgctgTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAg > 2:4535848/1‑76 (MQ=255) ctgTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCgg > 1:1766034/1‑76 (MQ=255) ctgTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑AGGGGC‑AAAGCCAACATCACCGGCACTAAAGCgg > 2:5359145/1‑76 (MQ=255) tgTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCggg < 1:2189306/76‑1 (MQ=255) tgTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCggg > 2:1104788/1‑76 (MQ=255) tttAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTc > 2:4160969/1‑76 (MQ=255) tttAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTc > 1:2289356/1‑76 (MQ=255) ttAAGCTGGGGGTGATAGCGGAATAAG‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCg < 2:3922379/76‑1 (MQ=255) ttAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCg > 2:3550675/1‑76 (MQ=255) ttAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCg < 1:3129612/76‑1 (MQ=255) ttAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCAGGTCg > 1:3291355/1‑76 (MQ=255) ttAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACCAAAGCGGGTCg > 2:3557185/1‑76 (MQ=255) tAAGGTGCGGGTGAGGGGGGAATAAA‑GGCGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGc < 2:2842789/76‑1 (MQ=255) tAAGCTGCTGGTGATGGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGc < 1:300553/76‑1 (MQ=255) tAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGc < 1:649881/76‑1 (MQ=255) aGCTGGTGGTGAGAGCGGAATAAG‑GGGGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCCCTAAAGCGGGTCGCAt < 2:1037715/76‑1 (MQ=255) aGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCAt < 1:1644509/76‑1 (MQ=255) aGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCAt < 2:4325332/76‑1 (MQ=255) aGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCAt > 2:1796429/1‑76 (MQ=255) gcggctgGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCCCCGGCACTAAAGCGGGTCGCATg < 2:3362059/73‑1 (MQ=255) ctgctgGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGa > 1:5443335/1‑76 (MQ=255) tgctgGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAc > 1:4044151/1‑76 (MQ=255) gctgGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAcc < 1:4480112/76‑1 (MQ=255) gctgGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAcc < 1:3741345/76‑1 (MQ=255) gtgGTGATAGCGAAATAAA‑GGATCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCg < 1:409992/75‑1 (MQ=255) ctgGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCg > 1:2898372/1‑76 (MQ=255) ctgGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCg > 2:5218209/1‑76 (MQ=255) tgGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGc < 1:4026817/76‑1 (MQ=255) tgGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGc > 2:3745178/1‑76 (MQ=255) ggTAATAGCGGAAGAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATCACCGcc < 1:375082/76‑1 (MQ=255) gTGATAGGGAAATAAA‑GGAGCCGATG‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCa < 1:5669496/76‑1 (MQ=255) tGATAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAg > 1:2314780/1‑76 (MQ=255) tGATAGCGGAATAAA‑GGAGCCAAAA‑C‑C‑GGGCAAAACCCACCATCCCCGGCACTAAACCGGGCCCCATGCCCGcaac > 2:1400067/1‑73 (MQ=255) tGATAGCGAAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAg < 1:4283838/76‑1 (MQ=255) tAGCGGAATAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACCAAAGCGGGCCGCATGACCGCCACCGt > 2:258430/1‑76 (MQ=255) ggggAAAAAG‑GGGGCGGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGGGGGTCGCATGACCGCCAGCGTTg < 2:3094627/74‑1 (MQ=255) ggggAAAAAAGGGAGCAGATG‑G‑CGGGGA‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGtt < 1:438927/74‑1 (MQ=255) agggAAAAAAGGGAGCCGATAGG‑G‑GGGA‑AAAGCCCCCATCCCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGtt < 1:2993424/74‑1 (MQ=255) cgcaaTAAA‑GGAGCCGATA‑GCC‑GGGA‑AAAGCCACCATCTCCGGCGCTAAAGGGCGTGGCATCACAGCCAGCGCTGc > 2:4390881/4‑76 (MQ=255) aaTAAA‑GGAGCCGATA‑GAG‑GGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGAGTTGTggg > 1:4556240/1‑76 (MQ=255) aaTAAA‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCggg > 1:1687711/1‑76 (MQ=255) aaa‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcga > 1:2042237/1‑76 (MQ=255) aa‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgat > 1:2586683/1‑76 (MQ=255) aa‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgag > 2:3672543/1‑75 (MQ=255) aa‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgag > 1:986464/1‑75 (MQ=255) a‑GGAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatg > 2:3048783/1‑76 (MQ=255) gAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcg > 1:1156027/1‑76 (MQ=255) gAGCCGATA‑G‑CGGGGC‑AAAGCCACCATCGCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGCTGCGGgcgatgcg > 2:4328/1‑76 (MQ=255) gCCGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcgat > 1:5007983/1‑76 (MQ=255) cGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTaaa < 2:1230618/36‑1 (MQ=37) cGATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTaaa > 1:1230618/1‑36 (MQ=37) cGAGA‑G‑C‑GGGAAAAACCCACCATCCCCGGCACAAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATgg < 1:5612288/76‑1 (MQ=255) gATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGa > 1:2753602/1‑76 (MQ=255) gATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGCTGCGGGCGCGGCGACggg > 2:2076298/1‑75 (MQ=255) gATA‑G‑CGGGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGCCCCCTGACCGCCCGCGCTCCGGGCGATGCGCTGGa > 2:5704895/1‑76 (MQ=255) | ACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAA‑GGAGCCGATA‑G‑C‑GGGC‑AAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGA > NC_000913/3123507‑3123651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 4 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |