BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,224,926	+C	coding (1052/1434 nt)	ygjI →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,224,924	1	.	C	99.3%	372.7 / NA	139	Y350Y (TAT→TAC)	ygjI	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (75/63); minor base A (1/0); total (76/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

TCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCA  >  NC_000913/3224848‑3224994

 ccaaCTCAACGAAAACGGCGGTCCGACGC‑GCGCAGCGCGGATCCAGTTCCTGATCGTCACCCCGCTGACGCTTAT‑c                                                                       >  2:3225786/3‑76 (MQ=255)

  gAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cc                                                                      <  2:5621229/76‑1 (MQ=255)

  gAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cc                                                                      <  1:3065/76‑1 (MQ=255)

  gAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cc                                                                      <  1:1667365/76‑1 (MQ=255)

  gAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cc                                                                      >  2:4496305/1‑76 (MQ=255)

   aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑ccc                                                                     <  1:1902039/76‑2 (MQ=255)

     cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccga                                                                   >  1:3647079/1‑73 (MQ=255)

     cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccga                                                                   <  2:2184929/76‑4 (MQ=255)

        aaCGAAACCGGCGTCCCGGCGC‑GCACAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGc                                                                 <  1:134306/76‑1 (MQ=255)

         gcGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCt                                                                <  2:1971012/75‑1 (MQ=255)

         aCGAAAACGGCTTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCt                                                                <  1:2802110/76‑1 (MQ=255)

          cGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                                               <  1:556122/76‑1 (MQ=255)

          cGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                                               <  2:3234411/76‑1 (MQ=255)

            aaaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATTGTCATCCCGCTGATGATTATCCCGATGCTCgg                                                             >  2:4482110/1‑76 (MQ=255)

             aaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccg                                                                    >  1:2522965/1‑65 (MQ=255)

             aaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccg                                                                    <  2:2522965/67‑3 (MQ=255)

              aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGtt                                                           <  2:1989991/76‑1 (MQ=255)

              aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGATGATGATTATCCCGATGCTCGGtt                                                           >  2:1510953/1‑76 (MQ=255)

              aaCGGCGTGCCGGTGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGtt                                                           <  1:3337836/76‑1 (MQ=255)

               aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTc                                                          >  1:5351817/1‑76 (MQ=255)

               aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTc                                                          >  1:5109949/1‑76 (MQ=255)

                cGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTcc                                                         >  2:3828108/1‑76 (MQ=255)

                 ggCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCa                                                        >  2:2529325/1‑76 (MQ=255)

                 ggCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCa                                                        >  2:3385526/1‑76 (MQ=255)

                   cGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAt                                                      <  1:4609159/76‑1 (MQ=255)

                    ggtCCGGCGA‑GCGCAGCGCGGATCCGGTTCCTGATCGTCTTCCCGCTGATGATTATACCGATGCTGGGATCCGATa                                                     >  1:4783817/3‑76 (MQ=255)

                    gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                                                     >  1:2315928/1‑76 (MQ=255)

                    gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                                                     <  1:3585528/76‑1 (MQ=255)

                    gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                                                     >  2:3471406/1‑76 (MQ=255)

                    gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGACGATTATCCCGATGCTCGGTTCCAATa                                                     >  1:1094085/1‑76 (MQ=255)

                     ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAc                                                    >  1:3173196/1‑76 (MQ=255)

                     ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAc                                                    >  1:5488333/1‑76 (MQ=255)

                     ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAc                                                    <  1:2907902/76‑1 (MQ=255)

                      tCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAcc                                                   >  2:5693238/1‑76 (MQ=255)

                         ggcgc‑gcgcaGCGTGTGTCCTGTTCCTGATAGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTg                                                <  2:1684305/76‑1 (MQ=255)

                         ggcgc‑gcgcaGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTg                                                <  2:5067130/76‑1 (MQ=255)

                         ggcgc‑gcgcaGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTg                                                >  2:1262703/1‑76 (MQ=255)

                         ggcgc‑gcgcaGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTg                                                >  1:3846351/1‑76 (MQ=255)

                         ggcgc‑gcgcaGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTg                                                >  1:1368315/1‑76 (MQ=255)

                         ggcgc‑gcgcaGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTg                                                >  2:3702082/1‑76 (MQ=255)

                            ggcgcgcAGCGTGGATCCAGTTCCTGAGCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCa                                              <  1:5781951/73‑1 (MQ=255)

                            cgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGCTGCTCGGCTCCAATACCGCGCa                                              >  2:2100163/4‑76 (MQ=255)

                            cgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCa                                              <  1:1706677/73‑1 (MQ=255)

                            cgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCa                                              <  2:4950171/73‑1 (MQ=255)

                            cgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCa                                              <  2:2801973/73‑1 (MQ=255)

                              cgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAgg                                            <  1:1415590/75‑1 (MQ=255)

                              cgcgcAGCGTGGATCCAGTTCCGGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAgg                                            <  2:508847/75‑1 (MQ=255)

                               gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGa                                           <  1:454790/76‑1 (MQ=255)

                                cgcAGCGTGGATCCAGTTCCTGATTGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAt                                          <  1:4482111/76‑1 (MQ=255)

                                cgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAt                                          >  2:4973547/1‑76 (MQ=255)

                                  cAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCt                                        >  1:5727569/1‑76 (MQ=255)

                                  cAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCt                                        <  2:1392381/76‑1 (MQ=255)

                                  cAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCt                                        <  1:489579/76‑1 (MQ=255)

                                  cAGCGTGGATCCAGCTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCt                                        <  1:1755949/76‑1 (MQ=255)

                                     cGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgat                                     <  1:1498926/76‑1 (MQ=255)

                                      gaggATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCcgatg                                    >  1:479986/3‑76 (MQ=255)

                                      gTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg                                    >  2:3630446/1‑76 (MQ=255)

                                       tGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatga                                   >  2:4722093/1‑76 (MQ=255)

                                        ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCAAATACCGTGCAGGATCTGATGaa                                  >  1:380172/1‑76 (MQ=255)

                                         taTCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAt                                 <  1:2100163/75‑1 (MQ=255)

                                         gATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAt                                 >  2:3997888/1‑76 (MQ=255)

                                          aTCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATa                                >  2:3008221/1‑76 (MQ=255)

                                           tCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAc                               >  2:5248492/1‑76 (MQ=255)

                                            ccAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAc                               <  2:2772177/75‑1 (MQ=255)

                                             cAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACta                             <  2:1843349/76‑1 (MQ=255)

                                             cAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACta                             <  1:1281137/76‑1 (MQ=255)

                                              aGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtat                            <  2:2102591/76‑1 (MQ=255)

                                              aGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtat                            <  1:2994515/76‑1 (MQ=255)

                                              aGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtat                            <  2:1326876/76‑1 (MQ=255)

                                               gTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatt                           <  1:5180693/76‑1 (MQ=255)

                                               gTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatt                           >  1:5450760/1‑76 (MQ=255)

                                                 tCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtattat                         <  1:1571867/76‑1 (MQ=255)

                                                  ccTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTattatt                        <  1:5712114/76‑1 (MQ=255)

                                                   cTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAttatta                       <  1:5367569/76‑1 (MQ=255)

                                                   cTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAttatta                       <  1:4496306/76‑1 (MQ=255)

                                                    ttaTCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTaa                      >  2:2260654/3‑76 (MQ=255)

                                                    tGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTaa                      >  1:2472215/1‑76 (MQ=255)

                                                     gATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                     >  1:4079073/1‑76 (MQ=255)

                                                     gATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                     >  2:4595984/1‑76 (MQ=255)

                                                      aTCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                    <  2:2572375/76‑1 (MQ=255)

                                                      aTCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                    >  2:1870403/1‑76 (MQ=255)

                                                      aTCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                    >  1:1208574/1‑76 (MQ=255)

                                                        cGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATg                  >  2:5836069/1‑76 (MQ=255)

                                                        cGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATg                  <  2:2558327/76‑1 (MQ=255)

                                                         gTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                 >  2:1417670/1‑76 (MQ=255)

                                                          tCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAc                >  2:1554247/1‑76 (MQ=255)

                                                           cATCCCGCTGATGATTATCCCGATGCCCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAcc               >  2:1435208/1‑76 (MQ=255)

                                                            aTCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccg              <  2:1376079/76‑1 (MQ=255)

                                                            aTCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccg              <  1:116085/76‑1 (MQ=255)

                                                             tCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATCCTATTATTAATATGAccgc             >  2:1694485/1‑76 (MQ=255)

                                                              cccGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgcc            >  2:3674987/1‑76 (MQ=255)

                                                               ccGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgccg           >  1:4346812/1‑76 (MQ=255)

                                                                cGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACcgccgc          >  1:3585062/1‑76 (MQ=255)

                                                                 gCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACcgccgcc         >  2:1783997/1‑75 (MQ=255)

                                                                 gCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCa         >  2:4743681/1‑76 (MQ=255)

                                                                    gatgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGGAGCg      >  1:3780856/1‑76 (MQ=255)

                                                                     atgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGt     >  1:4251656/1‑76 (MQ=255)

                                                                     atgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGt     >  2:5278496/1‑76 (MQ=255)

                                                                       gatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTcc   >  1:5627462/1‑76 (MQ=255)

                                                                        atTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCa  >  2:4327679/1‑76 (MQ=255)

TCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCA  >  NC_000913/3224848‑3224994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`