| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,257,838 | +G | coding (2222/2241 nt) | tdcE ← | putative formate acetyltransferase 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,257,837 | 1 | . | G | 98.7% | 417.2 / ‑1.4 | 154 | coding (2223/2241 nt) | tdcE | putative formate acetyltransferase 3 |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (66/85); total (68/86) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 169 total aligned reads displayed.
GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT > NC_000913/3257762‑3257907 | ggCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc < 2:5101066/76‑1 (MQ=255)ggCGTCAGAGCGCCTGGGTAAAGGGACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc > 2:2906847/1‑76 (MQ=255) gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑g < 1:5733368/76‑1 (MQ=255) gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑g < 2:3902813/76‑1 (MQ=255) cGTCAGAGCGCCTGGGCAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑gg > 2:2141354/1‑75 (MQ=255) gTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑ggc > 1:1203018/1‑74 (MQ=255) tCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑ggcg < 2:2746049/76‑4 (MQ=255) gagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAg < 2:525489/76‑1 (MQ=255) gcgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGcc < 2:4620980/76‑1 (MQ=255) cgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCa < 1:3872663/76‑1 (MQ=255) cgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCa > 2:5019990/1‑76 (MQ=255) cgcCTGGGTAAAGGGCCGTGACATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTGGAAGCTCACGG‑CGTAGCCa > 2:5045466/1‑76 (MQ=255) gcCTGGTTAAAGGAACGTGAAATAACAGCCTGTTGCCCTTCACGGGTCAGTGCGTCGAAGCGCACTG‑CGTCGCCag > 2:5321514/1‑76 (MQ=255) gcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCag > 2:251036/1‑76 (MQ=255) cTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCagag > 2:1374450/1‑76 (MQ=255) cTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCagag < 2:1175675/76‑1 (MQ=255) tGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAgaga > 2:2586356/1‑76 (MQ=255) tGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAgaga < 1:4274468/76‑1 (MQ=255) gggTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGac < 2:1494504/76‑1 (MQ=255) gggaaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAACCGCACGG‑CGTAGCCCGAGaca > 2:1215876/4‑76 (MQ=255) ggTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGaca > 1:2856937/1‑76 (MQ=255) ggTAAAGGTACGTGAAATAAAATCCTGTTGCTGTTCGCGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGaca > 1:5002645/1‑76 (MQ=255) tAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACg < 1:1894702/76‑1 (MQ=255) aaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCCGTGCGTTGAAGCGCACGG‑TGTAGCCAGAGACACgg > 1:5041599/1‑76 (MQ=255) aaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCTCACGG‑CGTAGCCAGAGACACGGa < 1:3348164/76‑1 (MQ=255) aGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGAt < 1:955601/76‑1 (MQ=255) ggTACGTGAAATAGCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGAtt < 1:3157963/76‑1 (MQ=255) ggTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGAtt < 2:4636238/76‑1 (MQ=255) ggTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGAtt > 1:2284567/1‑76 (MQ=255) ggTACGTGAAATAACATCCTGCTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGAtt > 1:3602103/1‑76 (MQ=38) gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTg > 2:5726427/1‑76 (MQ=255) gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCCGTAGCCAGAGCAACGGCTTg > 2:5556286/1‑76 (MQ=255) tACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGt > 2:4435533/1‑76 (MQ=255) aCGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCagag > 1:1526062/1‑64 (MQ=255) aCGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTc < 1:2742098/76‑1 (MQ=255) aCGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTc > 2:5424947/1‑76 (MQ=255) cGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCa < 2:4054527/76‑1 (MQ=255) gTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAg > 1:4358909/1‑76 (MQ=255) gTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAg > 1:5800316/1‑76 (MQ=255) gTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAg > 1:5567321/1‑76 (MQ=255) gTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAg > 1:5683078/1‑76 (MQ=255) tGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAgg > 2:1703753/1‑76 (MQ=255) tGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAgg < 2:4135719/76‑1 (MQ=255) tGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAgg < 1:407756/76‑1 (MQ=255) gAAATAACATCCTGTTGCTGTTCGCGGGTCAGTGCGTTGCAGCGCGC‑GTCGTAGCCAGAGACACGGCTTGTCAGGt < 2:2159189/76‑1 (MQ=255) gAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGt < 1:251036/76‑1 (MQ=255) aaaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGtt > 2:2681813/1‑76 (MQ=255) aaaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGtt > 2:4036785/1‑76 (MQ=255) aagaaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTa < 2:5165666/73‑1 (MQ=255) aTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAg > 1:4379264/1‑76 (MQ=255) aTAACATCCTGTTGCTGTTCACGGGTCAGTGAGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAg > 2:1936631/1‑76 (MQ=255) aCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGat > 1:1972303/1‑76 (MQ=255) cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGata < 1:82699/76‑1 (MQ=255) cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGata < 2:2061842/76‑1 (MQ=255) tCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAtt < 1:1631250/76‑1 (MQ=255) tCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAtt < 2:662089/76‑1 (MQ=255) ccTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttt < 1:5004050/76‑1 (MQ=255) ccTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttt < 1:3623664/76‑1 (MQ=255) tGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttttt < 2:5379791/76‑1 (MQ=255) tGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttttt < 2:1599000/76‑1 (MQ=255) gTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTc < 2:3424670/76‑1 (MQ=255) gTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTc < 1:3375098/76‑1 (MQ=255) ttGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTcc < 2:4403710/76‑1 (MQ=255) tGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCg < 1:3760472/76‑1 (MQ=255) gCTGTTCTCGGGTCAGTGCGTTGAAGCGCACGG‑CGTCGCCCGCGACACGGATTGCAAGGTCAGGACATTTCTTCgg > 1:918957/1‑76 (MQ=255) gCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg > 1:5229957/1‑76 (MQ=255) acTGTTCACGGGTCAGTGCGTTGATGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTcctg < 1:1949039/75‑3 (MQ=255) tGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgggg > 1:2034455/1‑75 (MQ=255) tGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGt > 2:1475770/1‑76 (MQ=255) tGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGt > 2:4508790/1‑76 (MQ=255) tGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGt > 1:2583653/1‑76 (MQ=255) tGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGt < 1:4704284/76‑1 (MQ=255) tGTTCACGGGTCAGAGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCACGTGAGGGTCTTTCTCCcggg > 2:36392/1‑75 (MQ=255) gTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTc < 2:3031640/70‑1 (MQ=255) gTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTc > 1:3031640/1‑70 (MQ=255) gTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTg > 1:3327521/1‑76 (MQ=255) ttCACGGGTCAGTGTGTTTCACCGCACGG‑CGTAGCCATATACACGGATTGTCATTTTAGGATATTTTTCCGGGTGc < 1:794134/76‑1 (MQ=16) ttCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCggggcc > 2:5530531/1‑73 (MQ=37) ttCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGc > 1:2225415/1‑76 (MQ=255) ttCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGc > 1:1035126/1‑76 (MQ=255) tCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCt > 1:2650889/1‑76 (MQ=255) aaCGTGTCAGTGCGTTGAAGCGCGCGG‑CATCGACATAATCACGGTTTGTCACGTTAAGATATCTTTCCTAGTGCTc < 2:2075198/75‑1 (MQ=255) cGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa < 2:4311299/76‑1 (MQ=255) cGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa > 2:2156611/1‑76 (MQ=255) cGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa < 1:1706270/76‑1 (MQ=255) gggTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGGTCGAt > 2:757267/1‑76 (MQ=38) gggTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAt < 1:3103798/76‑1 (MQ=255) ggTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATg > 2:1867740/1‑76 (MQ=255) ggTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATg < 1:1288513/76‑1 (MQ=255) gcggTGCGTGGAGGGGCACGG‑GGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGc < 2:228877/73‑1 (MQ=25) cAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGCTAGGATATTTTTCCGGGTGCTCGATGGCa > 1:4109406/1‑76 (MQ=38) aGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAt < 2:1712936/76‑1 (MQ=255) gTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATc > 1:4135516/1‑76 (MQ=255) gCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCca < 1:4036786/76‑1 (MQ=255) ttGCAGCGCCCGG‑CGTAGCCCGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGTGTCcagcc < 1:5432848/76‑2 (MQ=25) ttGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagca < 1:1585944/76‑1 (MQ=255) aaGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAgcagca < 1:3988033/76‑1 (MQ=255) aaGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAgcagca < 2:4324103/76‑1 (MQ=255) aGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAt < 2:3687598/76‑1 (MQ=255) cgcACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAttt < 1:1734585/76‑1 (MQ=255) | GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT > NC_000913/3257762‑3257907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |