| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,510,121 | +G | intergenic (‑358/+151) | yhfZ ← / ← trpS | hypothetical protein/tryptophan tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,510,120 | 1 | . | G | 96.4% | 211.6 / 2.7 | 84 | intergenic (‑357/+152) | yhfZ/trpS | hypothetical protein/tryptophan tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base G (51/29); total (51/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAAT > NC_000913/3510046‑3510190 | gagaTGTGGAAACGGCGAGGCACTTCACAGTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGCTTC‑CCC‑g > 1:1665620/1‑76 (MQ=255) agaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑gg > 2:2570013/1‑75 (MQ=255) agaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑gg > 1:3053577/1‑75 (MQ=255) agaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑gg < 2:4034266/76‑2 (MQ=255) aTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGGGGATAAAAAAACCGGGTTTC‑CCC‑ggtt < 2:3623376/76‑4 (MQ=255) aTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑ggtt > 1:5200779/1‑73 (MQ=255) aTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑ggtt < 1:2747591/76‑4 (MQ=255) aTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑ggtt > 1:5174696/1‑73 (MQ=255) ggAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCag > 1:4600316/1‑76 (MQ=255) gAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCaga > 2:1540152/1‑76 (MQ=255) aaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGt < 1:3045396/76‑1 (MQ=255) aaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGt < 1:495169/76‑1 (MQ=255) aCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGtg < 1:1051224/76‑1 (MQ=255) cGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGtga > 1:1232292/1‑76 (MQ=255) ggCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGtgat < 2:1094379/76‑1 (MQ=255) gCGAGGCAGTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGtgatc > 2:2049893/1‑75 (MQ=255) gCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGtgatg > 2:3948940/1‑76 (MQ=255) aGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATa > 2:5264717/1‑76 (MQ=255) gCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATaaa < 2:460859/76‑1 (MQ=255) cACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATaaaa > 1:4921836/1‑76 (MQ=255) cACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATaaaa > 2:1930310/1‑76 (MQ=255) aCTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAg > 1:2224093/1‑76 (MQ=255) cTTCACACTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGc < 1:3956157/76‑1 (MQ=255) ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCa > 1:3636900/1‑76 (MQ=255) ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCa < 1:5771408/76‑1 (MQ=255) tCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCaa < 2:2265150/76‑1 (MQ=255) tCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCaa > 1:1099175/1‑76 (MQ=255) acaTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCaaaa > 2:2633182/1‑76 (MQ=255) acaTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCaaaa < 1:267050/76‑1 (MQ=255) caTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAAt > 1:3473021/1‑76 (MQ=255) aTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAAtt < 1:3837559/76‑1 (MQ=255) ttttttCGGATTATTGCAAATGAGGAT‑AAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGc > 2:1643095/1‑76 (MQ=255) tttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGc > 1:4790522/1‑76 (MQ=255) ttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGcc < 1:1189200/76‑1 (MQ=255) ttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGGTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGcc > 1:5777848/1‑76 (MQ=255) ttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTg < 1:343873/76‑1 (MQ=255) tCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGa > 2:2265941/1‑76 (MQ=255) tCGGACTATTGAAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCACAGTGATGATAAAAGCAAAATTGCCTGa > 2:4011378/1‑76 (MQ=255) cGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAt > 1:3419179/1‑76 (MQ=255) cGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAt < 2:4243257/76‑1 (MQ=255) cGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAt > 2:4736048/1‑76 (MQ=255) gATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgc > 1:3906639/1‑76 (MQ=255) attattGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcg > 2:286072/1‑76 (MQ=255) ttattGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcgc > 1:4614971/1‑76 (MQ=255) attGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTa > 2:3258614/1‑76 (MQ=255) ttGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAc > 2:2672518/1‑76 (MQ=255) ttGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAc > 2:639628/1‑76 (MQ=255) ttGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAc > 2:5175001/1‑76 (MQ=255) ttGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAc < 2:1218413/76‑1 (MQ=255) tGCAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACg > 1:5713366/1‑76 (MQ=255) cAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCt < 1:3920675/76‑1 (MQ=255) cAAATGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCt < 1:5417816/76‑1 (MQ=255) aaaTGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCtt > 2:2931291/1‑76 (MQ=255) aaTGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTa > 2:1360427/1‑76 (MQ=255) aaTGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTa < 1:2530116/76‑1 (MQ=255) aTGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAt > 1:556783/1‑76 (MQ=255) tGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATc < 2:1738320/76‑1 (MQ=255) tGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATc > 2:5190899/1‑76 (MQ=255) tGAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATc > 2:801782/1‑76 (MQ=255) gAGGATAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCa < 1:3290226/76‑1 (MQ=255) gATAAAAAAACCGGGTCTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAGGCGCTACGCTTATCAGGc > 2:2833366/1‑76 (MQ=25) aTAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGcc < 2:1665620/76‑1 (MQ=255) aTAAAAAAACCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGcc > 1:4958650/1‑76 (MQ=255) aaaaaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTAc > 2:1779282/1‑76 (MQ=255) aaaaaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTAc > 1:4542799/1‑76 (MQ=255) aaaaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACa > 2:941255/1‑76 (MQ=255) aaaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAt < 2:5098440/76‑1 (MQ=255) aaaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAt > 1:2792490/1‑76 (MQ=255) aagccGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt < 1:1841111/73‑1 (MQ=21) aaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt > 1:1264330/1‑76 (MQ=255) aaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt < 1:1813668/76‑1 (MQ=255) aaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt < 2:1374431/76‑1 (MQ=255) aaaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt > 2:3164417/1‑76 (MQ=255) aaaCCGGGTGTCACCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt < 1:1841244/76‑1 (MQ=18) aaaCCGGGTGTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt < 1:1841214/76‑1 (MQ=21) aaaCCGGGTGTA‑CCAGGTGTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt < 1:1841221/76‑1 (MQ=17) aaCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAttt > 2:5292414/1‑76 (MQ=255) aCCTGGTTTC‑CTCTGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTCTCAGGCCTCCATTTc < 1:599592/76‑1 (MQ=18) aCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTc > 2:163676/1‑76 (MQ=255) aCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTc > 1:2698028/1‑76 (MQ=255) aCCGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTc < 2:3359827/76‑1 (MQ=255) ccGGGTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTcc > 1:3759670/1‑76 (MQ=255) gggTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCtt > 1:3427141/1‑76 (MQ=255) gggTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCtt < 1:5190900/76‑1 (MQ=255) ggTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTg > 2:1716162/1‑76 (MQ=255) ggTTCC‑CCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGTCCTTGc < 2:5200778/76‑1 (MQ=18) gTTTC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGc < 2:5174695/76‑1 (MQ=255) gTTCC‑CCG‑GTTTCAGAGTGATTATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGTTTCCTTGCa < 2:3532045/76‑1 (MQ=18) tttC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCa > 1:3058859/1‑76 (MQ=255) tttC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCa > 2:1448702/1‑76 (MQ=255) ttC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCaa > 1:676559/1‑76 (MQ=255) tC‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAat > 2:3720303/1‑76 (MQ=255) | GAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTC‑CCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAAT > NC_000913/3510046‑3510190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |