| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 177,105 | 0 | T | G | 42.0% | 80.7 / 54.8 | 112 | T174P (ACA→CCA) | yadS | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (10/55); new base G (47/0); total (57/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-22 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 115 total aligned reads displayed.
CGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGAT > NC_000913/177031‑177180 | cGGTAGCTTAAGAGCCAAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGtt < 1:2934288/76‑1 (MQ=255) gTAGCTTAAGATCCCAACAAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTc < 1:5628087/76‑1 (MQ=255) tAGCTTAAGATGCCACCGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTcc < 1:487835/76‑1 (MQ=255) tAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCGATAGCGTCACGACCATGCCCATCATACTGGCTGTTTcc < 2:1861531/76‑1 (MQ=255) aGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTTCa > 1:3105187/1‑76 (MQ=255) gCTTAAGATGCAAGCGAAGACCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑g < 2:3692147/76‑1 (MQ=255) cTTAGGAGGCCAACGAATGGCGGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑Gt < 2:523065/76‑1 (MQ=255) ttAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTTCC‑GTg > 1:148531/1‑76 (MQ=255) tAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCC‑GTgg > 1:4444754/1‑76 (MQ=255) aaGATGCCACCGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGt < 2:2751809/76‑1 (MQ=255) aGATCCCAACAAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTa < 1:5778192/76‑1 (MQ=255) acgCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACg < 1:3591608/74‑1 (MQ=255) aTGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTTCA‑GTGGTACg > 1:3975556/1‑76 (MQ=255) aTGCAAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTAca < 2:3600128/76‑2 (MQ=255) gcaaaCGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGa < 2:1788651/73‑1 (MQ=255) gCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGGTTCA‑GTGGGACGGa > 1:1133345/1‑76 (MQ=255) cAACGAATAGCCGCCAGCCGAATCAATAGCGTCGCGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGaaa < 2:4780046/76‑1 (MQ=255) acaaaTAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAAt < 1:2155014/73‑1 (MQ=255) aCGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAAt < 1:792196/76‑1 (MQ=255) aCGAATACCCCCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAAt < 1:2196043/76‑1 (MQ=255) gAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTTCA‑GGGGTACGGGAAAtgg > 2:62461/1‑75 (MQ=255) aaTAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAAtgtg < 1:3885579/76‑1 (MQ=255) aaTAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTATTTCCA‑GTGGTACGGAAAAtgtg < 1:2363383/76‑1 (MQ=255) aTAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTTCA‑GTGGGACGGGAAATgtgt > 1:3504986/1‑76 (MQ=255) tAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑CTGGTACGGAAAATGTGta > 2:2133698/1‑76 (MQ=255) tAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGGTCCA‑GTGATACGGGAAAAGTGta > 1:2352189/1‑76 (MQ=255) aGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGtaa < 1:4962260/76‑1 (MQ=255) aGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTTCC‑GTGGGACGGAAAAAGTGtaa > 1:2643122/1‑76 (MQ=255) aGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTCCC‑GTGGTACGGGAAATGTGgaa > 2:4507642/1‑76 (MQ=255) gccgccAGCCGAGTCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGtaat < 2:3995590/76‑1 (MQ=255) ccgccAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTCCA‑GTGGGACGGAAAAAGTGtaata > 2:4381907/1‑76 (MQ=255) cgccGGCCGAATCAATAGCGTCCCGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGtaataa < 2:106900/76‑1 (MQ=255) cgccAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGtaataa < 2:1580674/76‑1 (MQ=255) caccAGCCGAATCAATAGAGGCACGACCATGCCCATCACACTGGCTGTTTCCA‑GTGGTACGGAAACTGTGtaataa < 2:2232068/74‑1 (MQ=255) gccAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTTCC‑GTGGGACGGGAAATGTGgtataaa > 1:657297/1‑75 (MQ=255) gccAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTACA‑GTGGGACGGAAAATATGtaataaa > 1:4880110/1‑75 (MQ=255) gccAGCCGAATCAATAGCGTCACGACCATGACCATCATACTGGCTGTTTCCA‑GGGGGACGGGAAATGTGGAATAAg > 1:260278/1‑76 (MQ=255) ccAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGc < 1:3542611/76‑1 (MQ=255) ccAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGc < 1:5341342/76‑1 (MQ=255) ccAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGc < 2:1027144/76‑1 (MQ=255) acAGCCGAATCAAGAGCGGCACGACAAGGCCCATCAGACTGGCTGTTGCCA‑GGGGTACGGAAAAGGTGTAATAAGc < 2:1063261/75‑1 (MQ=255) cAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTCCA‑GTGGTACGGGAAATGTGtaataaaac > 1:5209281/1‑73 (MQ=255) aGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTTCA‑GTGGGACGGGAAATGTGTAATAAGACg > 1:2042706/1‑76 (MQ=255) gCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGt < 2:3687608/76‑1 (MQ=255) gCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGt < 2:896758/76‑1 (MQ=255) gCCGAATCAATAGCGTCACGACCATGCCCATCATACGGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGt < 2:3714604/76‑1 (MQ=255) ccGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTa < 1:2295062/76‑1 (MQ=255) cGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAg < 2:5056428/76‑1 (MQ=255) gAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGc < 2:4645545/76‑1 (MQ=255) gAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGc < 2:761114/76‑1 (MQ=255) gAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTTCA‑GTGGGACGGGAAATGTGTAATAAAACGTAGc > 1:1080321/1‑76 (MQ=255) aaTCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTCCGGACAATGTGTAATAAGCCGTAGCg < 1:1472854/76‑1 (MQ=255) aaTCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTTCA‑GTGGGAAGGAAAAAGGGGAATAAGACGGAGCg > 1:5745845/1‑76 (MQ=255) aaTCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTCCC‑CGGGGGCGGGAAAAGTGTAATAAGCCGCAGCg > 2:4937459/1‑76 (MQ=255) aaTCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTCCA‑GTGGTACGGGAAATGTGGAATAAGCCGTAGCg > 1:4135780/1‑76 (MQ=255) aaTCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTCCA‑GTGGGACGGGAAATGTGTAATAAGCCGCAGCg > 2:4767598/1‑76 (MQ=255) aTCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGt < 1:5812872/76‑1 (MQ=255) aTCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTCCC‑GTGGGGCGGGAAATGTGTAATAAGCCGGAgggg > 2:2439270/1‑73 (MQ=255) aTCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTCCC‑CTGGGACGGAAAATGTGTAATAAGCCCTAGCgg > 2:4635126/1‑75 (MQ=255) tCAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTTCA‑GTGGTACGGGAAATGTGTAATAAACCGTAGCGTg > 1:3809528/1‑76 (MQ=255) cAATAGCGTCACGACCATGCCCATCATACTGGCTGGTTCCC‑GTGGTACGGGAAATGTGTAATAAACCGGAGCgggg > 2:3857663/1‑76 (MQ=255) aaTAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGa < 2:5290382/76‑1 (MQ=255) aaTAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGa < 1:394099/76‑1 (MQ=255) aaTAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGGACGGAAAATGTGTAATAAACCGTAGCGTggg > 2:2349802/1‑75 (MQ=255) aaTAGCGTCACGACCATGCCCATCATACTGGCTGGTTCCA‑GTGGTACGGAAAATGTGTAATAAAACGTAGCGTGGa > 1:3252928/1‑76 (MQ=255) aaTAGCGTCACGACCATGCCCATCATACTGGCTGGTTCCA‑GTGGTACGGAAAATGTGTAATAAAACGTAGCGTGGa > 1:3195964/1‑76 (MQ=255) aTAGCGTCACGACCATGCCCATCATACTGGCTGGTTCCA‑GTGGTACGGAAAAAGTGTAATAAGCCGCAGCGCGGAc > 2:701172/1‑76 (MQ=255) aGCGTCACGACCATGCCCATCATACTGGCTGGTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGGGGGCaa > 1:3557048/1‑76 (MQ=255) aGCGGCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACaa < 2:4888422/76‑1 (MQ=255) gTCACGACCATGCCCATCATACTGGCTGGTTCCA‑GTGGGAAGGAAAATGTGTAATAAGGCGGAGCGTGGGCAAAAc > 1:4065227/1‑76 (MQ=255) gTCACGACCATGCCCATCATACTGGCTGGTTCCA‑GGGGTACGGGAAATGTGTAATAAACCGTAGCGGGGGAAATAc > 1:1190888/1‑76 (MQ=255) tCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATAcc < 2:3134472/76‑1 (MQ=255) tCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATAcc < 1:4007385/76‑1 (MQ=255) tCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATAcc < 1:2449605/76‑1 (MQ=255) cACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGGAAATGTGTAATAAGACGTAGGGTGGACCATAccc > 2:526718/1‑75 (MQ=255) cGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATAccgcc < 1:3089568/76‑1 (MQ=255) cGACCATGCCCATCATACTGGCTGGTTCCA‑GTGGGACGGGAAATGTGTAATAAGCCGTAGCGGGGGCAATAccgcc > 1:1293247/1‑76 (MQ=255) cGACCATGCCCATCATACTGGCTGGTTCCA‑GTGGGACGGAAAATGTGTAATAAACCGTAGCGTGGACAATAacgcc > 1:2545219/1‑76 (MQ=255) cGACCAGCCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATAccgcc < 2:2792473/76‑1 (MQ=255) gACCATGCCCATCATCCGGGCTTTTTCCA‑GGGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATAccgccg < 2:3654937/76‑1 (MQ=255) gACCATGCCCATCATACTGGCTGGTTTCA‑GTGGGACGGAAAATGTGTAATAAGCCGTAGCGGGGGGAATAccgccg > 1:1065240/1‑76 (MQ=255) gACCATGCCCATCATACTGGCTGGTTCCC‑GTGGTACGGAAAATGTGTAATAAGCCGTCGCGTGGACAAAAacgccg > 2:1813775/1‑76 (MQ=255) gACCATCCCCTCCATGCGGGCTGTTTCCA‑GTGGTACAGAAAAGTTGTAAAAAGCCATAGCGTGGACAATAccgccg < 2:5604530/76‑1 (MQ=255) cccATGCCCATCATGCGGGTTTTTTCCA‑GTGGTACGAAAATTGTGAAATAAGCCGTAGCGTGGACAACACCGCCGa < 1:2471552/75‑1 (MQ=255) aCCATGCCCATCATACTGGCTGGTTTCC‑GTGGTACGGGAAAAGTGTAAAAAACCCTAGCGGGGGCAAAAccgccca > 1:266936/1‑74 (MQ=255) cATGCCCATCATACTGGCTGGTTCCC‑GTGGGACGGGAAATGTGTAATAAACCGTAGCGGGGGCAAAAACGCCGaca > 1:2135129/1‑74 (MQ=255) cATGCCCATCATACTGGCTGGTTCCA‑GTGGGACGGGAAATGTGTAATAAACCGTAGCGTGGACAATACCGCCGata > 1:3531572/1‑76 (MQ=255) aTGCCCATCATACTGGCTGGTTCCA‑GTGGTACGGGAAATGTGTAATAAGCCGTAGCGTGGGCAATACCGCCGataa > 1:4652616/1‑76 (MQ=255) catcatACTGGCTGGTTTCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATAACCCCGctaataacg > 2:2839963/1‑73 (MQ=255) catcatACTGGCTGGTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACCATACCGCCGATAATAccg > 2:3081385/1‑74 (MQ=255) tcatACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGGGGACAATCACCCCGCAAATACACGc > 2:603773/1‑76 (MQ=255) cgtACGGGCTGTTTCGA‑GGGGTAAGAAAAATGTGAAATAAGCGGGAGGGTGGAAAATCCCGCCGATAATACAGGCa < 2:4452529/74‑1 (MQ=255) atACTGGCTGGTTCCC‑GTGGGACGGGAAATGTGTAATAAGCCGTAGGGTGGGCAATACCCCCGATAATACAGGGAg > 1:5531091/1‑76 (MQ=255) atACTGG‑TGTTTTCAGGGGGTAGGAAAAATGGAAAAAAAGCCGGAGGGGGGACAATACCGCCAATAATACAGGCAg < 2:1097352/76‑1 (MQ=255) aCTGGCTGGTTCCC‑GTGGTACGGGAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAggt > 1:4130580/1‑74 (MQ=255) cTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGGTGCgca > 1:343107/1‑74 (MQ=255) tGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATAccg > 1:2999112/1‑52 (MQ=255) tGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATAccg < 2:2999112/52‑1 (MQ=255) gTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGGTGCGCAGa > 2:4250134/1‑76 (MQ=255) tttCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGAt > 1:5078770/1‑76 (MQ=255) | CGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCA‑GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGAT > NC_000913/177031‑177180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 4 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |