| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 244,133 | 0 | G | A | 54.9% | 158.3 / inf | 395 | intergenic (+12/‑194) | gmhA/yafJ | phosphoheptose isomerase/putative amidotransferase |
| Reads supporting (aligned to +/- strand): ref base G (96/81); new base A (113/102); total (211/184) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.47e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 403 total aligned reads displayed.
GGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGC > NC_000913/244058‑244208 | agaTTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGcc > 1:1025473/1‑76 (MQ=255) agaTTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCT‑GCGGAGGCCg > 2:781661/1‑76 (MQ=255) aTTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCgg < 2:5114014/76‑1 (MQ=255) ttCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGa < 1:2941851/76‑1 (MQ=255) tCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGAt < 1:5587893/76‑1 (MQ=255) acaTTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATaa < 1:5018855/76‑1 (MQ=255) tAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACg > 1:1333406/1‑76 (MQ=255) taGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGCCCGGATAAGACGtt > 2:282415/2‑76 (MQ=255) aGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGctt > 2:1115755/1‑76 (MQ=255) gTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTa > 1:4998410/1‑76 (MQ=255) gTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGAAGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTa < 2:3257220/76‑1 (MQ=255) tGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTAc < 2:1529706/76‑1 (MQ=255) aTCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTAcgc < 2:4125839/76‑1 (MQ=255) tCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTAcgcc > 1:4221615/1‑76 (MQ=255) cATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTAcgccgc < 2:2966121/76‑1 (MQ=255) atatCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCa < 1:4850500/76‑1 (MQ=255) atCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATc < 1:4369358/76‑1 (MQ=255) cTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCgg < 1:231681/76‑1 (MQ=255) gATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCa > 2:4404245/1‑76 (MQ=255) aTCCAGTTGATTGAAAAAGAGATGGTTAAGCAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAt > 1:1216377/1‑76 (MQ=255) tatgcgcgtcttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCAtt > 1:5420391‑M1/40‑76 (MQ=255) tatgcgcgtcttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCAtt < 2:1867994‑M1/37‑1 (MQ=255) aGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCCGCATTtgg > 2:1777678/1‑75 (MQ=255) gTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTtgtg > 2:3178780/1‑76 (MQ=255) gTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTtgtg > 2:3684467/1‑76 (MQ=255) gcgtcttgtcaggtctacggtttatgggcgaagtGTAGGCCGGATAGGCCGTTTACGCCGCATCGGGCATTTGTgc > 1:2587534‑M1/35‑76 (MQ=255) gcgtcttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgc < 1:1785966‑M1/42‑1 (MQ=255) cgtcttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcg < 1:3943617‑M1/43‑1 (MQ=255) cgtcttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcg > 1:2134025‑M1/34‑76 (MQ=255) gtcttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcgc < 1:3717828‑M1/44‑1 (MQ=255) gtcttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcgc < 2:3577314‑M1/44‑1 (MQ=255) gtcttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcgc < 2:5496261‑M1/44‑1 (MQ=255) gtcttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcgc > 1:5077695‑M1/33‑76 (MQ=255) tcttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCCGCATTTGtgcgcg < 2:1313756‑M1/45‑2 (MQ=255) gtgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctg < 2:4489849/75‑1 (MQ=255) cttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctg > 1:1413066‑M1/31‑76 (MQ=255) cttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctg > 2:3030060‑M1/31‑76 (MQ=255) ttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctga > 2:1650640‑M1/30‑76 (MQ=255) ttatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctga < 1:569386‑M1/47‑1 (MQ=255) tatcaggcctgcggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgat > 2:5347369‑M1/29‑76 (MQ=255) tatcaggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgat > 2:4290449‑M1/29‑76 (MQ=255) gaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgat > 2:736674/1‑76 (MQ=255) gaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgat < 2:2020854/76‑1 (MQ=255) aaaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatg < 1:3880464/76‑1 (MQ=255) aggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct > 1:5234861‑M1/25‑76 (MQ=255) aggcctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct > 1:5180846‑M1/25‑76 (MQ=255) aggcatacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgacgcct < 2:5788765‑M1/52‑1 (MQ=255) agagATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctg > 2:639436/1‑76 (MQ=255) gaTGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatg > 1:3379482/1‑76 (MQ=255) gaTGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatg < 1:3308005/76‑1 (MQ=255) ctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatg < 2:3315800‑M1/56‑1 (MQ=255) ctacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatg < 2:4829662‑M1/56‑1 (MQ=255) tgcggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatgc > 2:889972‑M1/20‑76 (MQ=255) tacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatgc < 1:985542‑M1/57‑1 (MQ=255) tacggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatgc < 1:1158480‑M1/57‑1 (MQ=255) aTGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatgc < 1:3162068/76‑1 (MQ=255) tGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCg > 1:4798218/1‑76 (MQ=255) acggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCg > 1:4512151‑M1/19‑76 (MQ=255) ggTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGCTGCCTGATGCGa > 2:4643746/1‑76 (MQ=255) cggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGa > 1:1103972‑M1/18‑76 (MQ=255) cggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGa > 1:3462139‑M1/18‑76 (MQ=255) cggtatatggggtgagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGa > 2:2269752‑M1/18‑76 (MQ=255) ggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGAc < 1:5338090‑M1/60‑1 (MQ=255) ggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGAc > 1:4120589‑M1/17‑76 (MQ=255) ggtttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGAc < 1:713787‑M1/60‑1 (MQ=255) gTTAAGTAAGTCTGGCGTAGGCCGGATGAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGAc > 1:3660096/1‑76 (MQ=255) gTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGAc < 2:4922183/76‑1 (MQ=255) gTTAAGTAAGTCTCGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGAc < 2:2216867/76‑1 (MQ=255) tttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGc < 1:1572878‑M1/62‑1 (MQ=255) taagtaagTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGc > 1:5828093/1‑76 (MQ=255) ttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCt > 2:5562188‑M1/14‑76 (MQ=255) ttatgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCt > 1:1623506‑M1/14‑76 (MQ=255) aagtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCt < 2:5009359/76‑1 (MQ=255) agtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTg < 1:3065784/76‑1 (MQ=255) agtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTg < 2:3795701/76‑1 (MQ=255) atgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGa < 1:1522548‑M1/65‑1 (MQ=255) atgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCCGATGCGACGCtgg < 2:2675567‑M1/65‑2 (MQ=255) tgggcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAc < 1:3237579‑M1/66‑1 (MQ=255) aagtCTGGCGTAGGCCGGATAAGACGTTTCCGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGCCGCTGAcg > 2:5825054/1‑76 (MQ=255) ggcggagtGTGGGCCGGATGAGGCGTTTAGGCCGCATCCGGCATTGGTGCGCTGATGCCTGATGCGACGCTGAcgc > 2:471736‑M1/9‑76 (MQ=255) ggcgaggtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcgc > 2:3512872‑M1/9‑76 (MQ=255) gcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcgcg < 2:2449415‑M1/69‑1 (MQ=255) gcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcgcg < 1:844123‑M1/69‑1 (MQ=255) gcgaagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcgcg > 1:5497956‑M1/8‑76 (MQ=255) tCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGt > 1:802119/1‑76 (MQ=255) tCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGt < 1:1932850/76‑1 (MQ=255) gcTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGt < 2:3093288/75‑1 (MQ=255) tGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCt > 2:254248/1‑76 (MQ=255) aagtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCt < 2:2134466‑M1/72‑1 (MQ=255) agtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCtt < 2:3437568‑M1/73‑1 (MQ=255) gtGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTa < 1:916156‑M1/74‑1 (MQ=255) gCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTa > 1:4605857/1‑76 (MQ=255) gTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATc > 2:4350837/1‑76 (MQ=255) gTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATc > 2:3847924/1‑76 (MQ=255) gTAGACCGGAGAAGACGTTTACGCCGCATCCGGCATTTGGGCGCTGATGCCTGATCCGACGCGGACGCGTCGTATc > 2:2165758/1‑76 (MQ=18) tAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCa > 2:1376583/1‑76 (MQ=255) tAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCa < 1:2764261/76‑1 (MQ=255) aGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCAt < 1:2505638/76‑1 (MQ=255) aGGCCAGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTCTCac > 2:2387578/1‑75 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGc > 2:5145772/1‑76 (MQ=255) | GGAGATTCACATTAAAGTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGC > NC_000913/244058‑244208 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |