| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 301,410 | 0 | C | T | 57.6% | 309.1 / inf | 513 | E130K (GAG→AAG) | yagT | putative xanthine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (109/107); new base T (165/129); total (274/239) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 538 total aligned reads displayed.
CATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCC > NC_000913/301335‑301485 | catcatGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGAtctc < 1:3473876/76‑1 (MQ=255)catcatGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGAtctc < 1:2387376/76‑1 (MQ=255) atcatGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCg < 1:5677509/76‑1 (MQ=255) atcatGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCg < 1:2786103/76‑1 (MQ=255) catGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGc > 2:3096551/1‑76 (MQ=255) tGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGccc > 1:2000709/1‑76 (MQ=255) gCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGcccc > 1:1503747/1‑76 (MQ=255) gCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGcccc > 2:2968595/1‑76 (MQ=255) gCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGCACTTCAATGGTGGTGATCTTGGcccc > 1:2200287/1‑76 (MQ=255) ttGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCCATGGTCGCGATCTCTGcccccc > 2:5699579/1‑75 (MQ=255) ttGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCt < 2:4847563/76‑1 (MQ=255) ttGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCt > 1:3353160/1‑76 (MQ=255) ttGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCt < 1:4256612/76‑1 (MQ=255) gATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGa < 2:3646810/76‑1 (MQ=255) gATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGa > 1:4644003/1‑76 (MQ=255) tAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATg > 2:3808077/1‑76 (MQ=255) aaaGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCACCTGATGc > 1:4493079/1‑76 (MQ=255) aaaGGACGCCTGCATGGGGTGAAGATGAGCTGGCGAACCCACACCTGCAAGGGGGGTGATCTCGGGCCCCCGATga > 1:669541/1‑75 (MQ=255) aGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCAt < 2:2808193/76‑1 (MQ=255) ggCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATg < 1:283368/76‑1 (MQ=255) ccgccTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGAc < 1:5343731/76‑1 (MQ=255) ccgccTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGAc < 1:3317354/76‑1 (MQ=255) cgccTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACt > 1:817790/1‑76 (MQ=255) cgccTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACt < 1:4791286/76‑1 (MQ=255) cgccTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACt > 2:4418275/1‑76 (MQ=255) gccTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTg < 2:345323/76‑1 (MQ=255) ccTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGc > 2:2206609/1‑76 (MQ=255) gCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAg < 2:5434248/76‑1 (MQ=255) cATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGc < 1:4890878/76‑1 (MQ=255) cATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGc < 1:4087314/76‑1 (MQ=255) cATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGc > 1:5845519/1‑76 (MQ=255) aTGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCCTGACTGCAAGCg > 2:5574935/1‑76 (MQ=255) gggTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTca < 2:2477058/76‑1 (MQ=255) gggTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTca < 2:5382457/76‑1 (MQ=255) gggTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTca < 1:5027156/76‑1 (MQ=255) gggTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCAGCCCCCTGATGCATGACTGCAAGCGTca > 1:1910498/1‑76 (MQ=255) ggTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTcag < 1:293602/76‑1 (MQ=255) gTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTcagg < 1:1361704/76‑1 (MQ=255) tGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTcaggc < 1:5749513/76‑1 (MQ=255) gAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGCCTGCAAGCGTcaggca < 2:3337290/76‑1 (MQ=255) gAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTcaggca > 2:1203331/1‑76 (MQ=255) gAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTcaggca < 1:5262780/76‑1 (MQ=255) gATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCaggcaggc > 1:3949270/1‑76 (MQ=255) gATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCaggcaggc < 2:835931/76‑1 (MQ=255) aTTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAggcaggca < 2:328273/76‑1 (MQ=255) ttATCTGGCTAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCAt > 1:1690919/1‑76 (MQ=255) ttATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCAt > 1:1582871/1‑76 (MQ=255) tATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCAtt > 1:1755976/1‑76 (MQ=255) tATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGCCCGGCAtt > 2:393444/1‑76 (MQ=255) tATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCAtt < 1:3676137/76‑1 (MQ=255) aTCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTa > 1:1136024/1‑76 (MQ=255) tCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCAttca < 1:37900/76‑3 (MQ=255) tGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGc > 1:5373855/1‑76 (MQ=255) tGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGc < 2:2239893/76‑1 (MQ=255) gCGAACCCTGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCt < 2:5001407/76‑1 (MQ=255) gCGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGcca < 2:1523323/76‑2 (MQ=255) gCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCt > 2:3544875/1‑76 (MQ=255) cGAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTg > 2:2681487/1‑76 (MQ=255) cGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTg > 1:3021093/1‑76 (MQ=255) cGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTg > 2:5264598/1‑76 (MQ=255) gAACCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCCACCCCCAGCCCGGCATTAAGCCtcc > 2:107569/1‑74 (MQ=255) gAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGTCTGc > 1:4359489/1‑76 (MQ=255) gAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCtcc > 2:5217055/1‑74 (MQ=255) aaCCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCg > 1:2569721/1‑76 (MQ=255) aaCCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCg > 2:4242368/1‑76 (MQ=255) aaCCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCg > 1:3157114/1‑76 (MQ=255) aaCCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCg > 2:706721/1‑76 (MQ=255) aCCCAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGa > 1:74415/1‑76 (MQ=255) cccAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGAc > 1:938999/1‑76 (MQ=255) ccAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGaac > 2:2552854/1‑74 (MQ=255) ccAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGAcc > 1:519083/1‑76 (MQ=255) cAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCa > 1:1231105/1‑76 (MQ=255) cAGGCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCa > 2:1920757/1‑76 (MQ=255) gCCTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTg > 2:4059629/1‑76 (MQ=255) gCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTg > 2:1464059/1‑76 (MQ=255) gCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCACTg > 2:2399775/1‑76 (MQ=255) ccTTCAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGa < 2:4744346/76‑1 (MQ=255) ccTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGa < 1:2657537/76‑1 (MQ=255) tcgaTGGTGGTGATGTTGGCCGCCTGGTGCATGAGTTCAATCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCa < 2:1283947/73‑1 (MQ=255) cAATGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTca < 1:5136734/45‑1 (MQ=255) cAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAACCGTCAGCCAGGCATTAAGCCTCCCACCATCGAcccc > 2:139172/1‑74 (MQ=255) aaTGGTGGTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCCGCGACCCTTGACCCGc > 2:1730078/1‑76 (MQ=255) aaTGGTGGTGATCTTCGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCCGCGACCATTGACCAGc > 2:3731346/1‑76 (MQ=255) aTGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCa > 1:2474464/1‑76 (MQ=255) tggtggTGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCAc < 1:3600203/76‑1 (MQ=255) ggtggtGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGCCCATTGACCAGCACg < 2:2063858/76‑1 (MQ=255) ggtggtGATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACg > 1:2090708/1‑76 (MQ=255) tggtgATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGt < 1:2274146/76‑1 (MQ=255) tggtgATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGt < 1:4550671/76‑1 (MQ=255) ggtgATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTa > 2:1784823/1‑76 (MQ=255) ggtgATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCACCACCGtc > 2:5092402/1‑75 (MQ=255) ggtgATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGCTa > 2:461635/1‑76 (MQ=255) gaggATCTTGGCCCGCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTa < 2:1609202/73‑1 (MQ=255) gtgATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTAc < 2:1986440/76‑1 (MQ=255) tgATCTTGGCCCCCTGATGCATGACTGCAAGCGGCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACa < 2:2934111/76‑1 (MQ=255) gATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAg > 2:2325445/1‑76 (MQ=255) gATCTTGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAg > 1:1020505/1‑76 (MQ=255) gATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGAACGGTACAg > 2:5819157/1‑76 (MQ=255) tcGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCAGTGACCAGCACGGTACAGGCTc > 1:5494417/1‑76 (MQ=255) cGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGCACACGCTcc > 2:3208723/1‑76 (MQ=255) | CATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCC > NC_000913/301335‑301485 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |