| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 366,797 | 0 | G | A | 66.7% | 663.8 / inf | 589 | intergenic (‑54/+14) | lacI/mhpR | LacI/transcriptional regulator for mhp operon |
| Reads supporting (aligned to +/- strand): ref base G (95/99); new base A (198/191); total (293/296) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 607 total aligned reads displayed.
TACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTT > NC_000913/366723‑366872 | tACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGACAGGTTTTgcgc > 2:4939813/1‑76 (MQ=255)tACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTgcgc < 2:4238435/76‑1 (MQ=255)tACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTgcgc > 1:2524448/1‑76 (MQ=255)tACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTgcgc < 1:2379445/76‑1 (MQ=255) tGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCAt < 1:5814232/76‑1 (MQ=255) tGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAt > 1:455671/1‑76 (MQ=255) gTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTc > 1:1547203/1‑76 (MQ=255) tttCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCg < 1:634325/76‑1 (MQ=255) ttCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGa > 1:5659606/1‑76 (MQ=255) ttCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGa < 2:4121820/76‑1 (MQ=255) tCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGAt < 1:1378261/76‑1 (MQ=255) tCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGAt > 2:3606175/1‑76 (MQ=255) acaTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATgg > 2:5220775/1‑76 (MQ=255) caTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgt < 2:5102491/76‑1 (MQ=255) aTTCACCACCCTGAACTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtg > 2:3091150/1‑76 (MQ=255) ttCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGgtgt < 1:2635157/76‑1 (MQ=255) tCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTc < 1:1249404/76‑1 (MQ=255) tCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTc > 2:4718190/1‑76 (MQ=255) caccacCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCa > 2:3409631/1‑76 (MQ=255) caccacCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCa < 2:1954968/76‑1 (MQ=255) caccacCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCa > 1:4974444/1‑76 (MQ=255) caccacCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCa > 2:2580523/1‑76 (MQ=255) acctccCTGAATTTACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCaa < 1:274699/76‑1 (MQ=255) caccCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACg < 1:3939991/76‑1 (MQ=255) accCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGt > 2:5649117/1‑76 (MQ=255) cccTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTa < 1:3513395/76‑1 (MQ=255) tGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAat < 2:2681335/76‑1 (MQ=255) gAATTGCCTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAatg < 2:5371534/76‑1 (MQ=255) aaTTGACTCTCTGCCGGGCGCTATCATGCCATACCGCGAGAGGTTTTGCACCATTCGATGGTGTCAACGTAAatgc < 2:1223429/76‑1 (MQ=255) ttGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAatgcat < 2:1895969/76‑1 (MQ=255) tGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAatgcatg > 2:4321903/1‑76 (MQ=255) tGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAatgcatg > 2:1509700/1‑76 (MQ=255) tGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAatgcatg < 2:1434795/76‑1 (MQ=255) aCTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGcc > 2:183072/1‑76 (MQ=255) aCTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGcc > 1:78334/1‑76 (MQ=255) aCTCTCTGCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGcc < 2:841798/76‑1 (MQ=255) ctctctTCCGTGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCg < 1:3309020/76‑1 (MQ=255) ctctctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCg > 2:5498819/1‑76 (MQ=255) ctctctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCg < 2:3676009/76‑1 (MQ=255) tctctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGc > 1:3058062/1‑76 (MQ=255) tctctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGCAAATGCATGCCGc > 1:4099878/1‑76 (MQ=255) ctctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCt > 2:2262684/1‑76 (MQ=255) ctctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTATATGCATGCCGCt > 2:2047810/1‑76 (MQ=255) ctctCCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCt < 2:5774456/76‑1 (MQ=255) ctTCCGGGCGCTGGGATGCCATACCGCGATAGGTTTTGCCTCATTCGCTGGTTTCTGCGTAAATTCATGCCGCttt < 2:1386363/76‑2 (MQ=255) ctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTc < 1:1080544/76‑1 (MQ=255) ttCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCg > 1:3107610/1‑76 (MQ=255) ttCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCg < 1:2006696/76‑1 (MQ=255) tCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTCCGc > 2:463880/1‑76 (MQ=255) tCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGc < 1:1915196/76‑1 (MQ=255) tCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGc > 1:4498222/1‑76 (MQ=255) tCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGc > 2:1622992/1‑76 (MQ=255) ctggCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCt > 2:2906831/3‑76 (MQ=255) cGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCt > 1:169473/1‑76 (MQ=255) cGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCt > 2:2375691/1‑76 (MQ=255) gggCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCtt > 2:1314079/1‑76 (MQ=255) gggCGCTATCAGGCCATCCCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCtt < 2:478789/76‑1 (MQ=255) gcgcTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTcc < 2:3002275/76‑1 (MQ=255) gcgcTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTcc > 2:650451/1‑76 (MQ=255) gcgcTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTcc > 1:1462846/1‑76 (MQ=255) gcgcTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTcc < 2:3177745/76‑1 (MQ=255) cgcTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGTCGCTTCGCCTTCCg > 2:2742144/1‑76 (MQ=255) cgcTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCg > 2:5417332/1‑76 (MQ=255) cgcTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCg > 2:2495734/1‑76 (MQ=255) gcTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCgg > 2:4977423/1‑76 (MQ=255) gcTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCgg > 2:3953452/1‑76 (MQ=255) cTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGTTTCGCCTTCCGGc < 1:4823486/76‑1 (MQ=255) cTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGc < 1:5111611/76‑1 (MQ=255) tATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGcc > 1:3671028/1‑76 (MQ=255) tATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGcc > 2:1756618/1‑76 (MQ=255) tCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGccac < 1:183072/76‑1 (MQ=255) tCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGccac < 1:2259322/76‑1 (MQ=255) cATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGccacc > 2:3910462/1‑76 (MQ=255) aTGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGccacca < 1:5372329/76‑1 (MQ=255) aTGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGccacca < 1:2824417/76‑1 (MQ=255) gCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGa < 1:4914812/76‑1 (MQ=255) cATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAAt > 1:4626529/1‑76 (MQ=255) cATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGACACCAGAAt > 1:3364966/1‑76 (MQ=255) tACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATac > 2:4531067/1‑75 (MQ=255) gaCCGCGAATGGTTTTGCGCCATTCGATGGTGTCACCGTAAATGCACGCCGCTTCGGCTTTCGGCCCCCAGAATAg < 2:2342655/75‑1 (MQ=255) aCCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGc > 1:2148295/1‑76 (MQ=255) aCCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGc > 1:2995100/1‑76 (MQ=255) aCCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGc > 1:4710323/1‑76 (MQ=255) ccGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTc > 2:3541560/1‑53 (MQ=255) ccGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGcc < 2:3768372/76‑1 (MQ=255) cgcgAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCt < 1:5596446/76‑1 (MQ=255) cgcgAAAGGGTGTGCGCCATTCGATGGTGTCAACGTACATGCATGCCGCTTCGCCTTCCGGCGACCAGAACAGcgt > 1:3829035/1‑74 (MQ=255) gcgAAAGTGTTTGCACAATTCGATGGGGTCAACGTACATGACTGCCGCTTCGCCTTCCGGCCACCAGAATAGCCtt < 1:5505255/76‑2 (MQ=255) cgAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGc > 1:993795/1‑76 (MQ=255) gAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCg > 1:3716976/1‑76 (MQ=255) gAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCg < 2:5318686/76‑1 (MQ=255) aaaGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGa < 1:5220776/76‑1 (MQ=255) aGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAACAGCCTGCgcat > 2:730582/1‑73 (MQ=255) ggTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTc < 1:1005811/76‑1 (MQ=255) ggTTTTGCACCATTCGATGGTGTCAACGTAAATGCCTGCCGCTTCTCCTTGCGGCCACCAGAATAGCCTGCGATTc < 2:5157325/76‑1 (MQ=255) tttGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAg < 1:4287842/63‑1 (MQ=255) tttGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAAc > 2:3247943/1‑76 (MQ=255) ttGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAAcc < 1:3256809/76‑1 (MQ=255) tggcgcCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGGCTGCGATTCAAcc < 2:1117891/74‑1 (MQ=255) tGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAAccc < 2:4459603/76‑1 (MQ=255) | TACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTT > NC_000913/366723‑366872 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |